IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ yun-2013a-1 LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25. in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Yun H.J., Park J., Ho D.H., Kim H., Kim C.H., Oh H., Ga I., Seo H., Chang S., Son I., Seol W. Synapse - Interactions of proteins with an established role in the presynapse. GST-SNAP-25:- a gift from Sheng Z.H. This construct had been entered as Rattine in origin - see Yokoyama CT et al., 1997 PMID: 9278528. The Interactions shown in figure 5 (syntaxin IP) cannot be included due to the lack of specificity of the anti-syntaxin antibody used. Results from the in vitro Kinase assay (Figure 1A) have not been included as the purification (IP only) may not have been sufficient. sonih@wku.ac.kr,wseol@wmcsb.co.kr Exp. Mol. Med. (1226-3613) 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease yun-2013a-2 LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Yun H.J., Park J., Ho D.H., Kim H., Kim C.H., Oh H., Ga I., Seo H., Chang S., Son I., Seol W. Synapse - Interactions of proteins with an established role in the presynapse. GST-SNAP-25:- a gift from Sheng Z.H. This construct had been entered as Rattine in origin - see Yokoyama CT et al., 1997 PMID: 9278528. The Interactions shown in figure 5 (syntaxin IP) cannot be included due to the lack of specificity of the anti-syntaxin antibody used. Results from the in vitro Kinase assay (Figure 1A) have not been included as the purification (IP only) may not have been sufficient. sonih@wku.ac.kr,wseol@wmcsb.co.kr Exp. Mol. Med. (1226-3613) 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease yun-2013a-3 LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yun H.J., Park J., Ho D.H., Kim H., Kim C.H., Oh H., Ga I., Seo H., Chang S., Son I., Seol W. Synapse - Interactions of proteins with an established role in the presynapse. GST-SNAP-25:- a gift from Sheng Z.H. This construct had been entered as Rattine in origin - see Yokoyama CT et al., 1997 PMID: 9278528. The Interactions shown in figure 5 (syntaxin IP) cannot be included due to the lack of specificity of the anti-syntaxin antibody used. Results from the in vitro Kinase assay (Figure 1A) have not been included as the purification (IP only) may not have been sufficient. sonih@wku.ac.kr,wseol@wmcsb.co.kr Exp. Mol. Med. (1226-3613) 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease yun-2013a-4 LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25. human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yun H.J., Park J., Ho D.H., Kim H., Kim C.H., Oh H., Ga I., Seo H., Chang S., Son I., Seol W. Synapse - Interactions of proteins with an established role in the presynapse. GST-SNAP-25:- a gift from Sheng Z.H. This construct had been entered as Rattine in origin - see Yokoyama CT et al., 1997 PMID: 9278528. The Interactions shown in figure 5 (syntaxin IP) cannot be included due to the lack of specificity of the anti-syntaxin antibody used. Results from the in vitro Kinase assay (Figure 1A) have not been included as the purification (IP only) may not have been sufficient. sonih@wku.ac.kr,wseol@wmcsb.co.kr Exp. Mol. Med. (1226-3613) 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease yun-2013a-5 LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yun H.J., Park J., Ho D.H., Kim H., Kim C.H., Oh H., Ga I., Seo H., Chang S., Son I., Seol W. Synapse - Interactions of proteins with an established role in the presynapse. GST-SNAP-25:- a gift from Sheng Z.H. This construct had been entered as Rattine in origin - see Yokoyama CT et al., 1997 PMID: 9278528. The Interactions shown in figure 5 (syntaxin IP) cannot be included due to the lack of specificity of the anti-syntaxin antibody used. Results from the in vitro Kinase assay (Figure 1A) have not been included as the purification (IP only) may not have been sufficient. sonih@wku.ac.kr,wseol@wmcsb.co.kr Exp. Mol. Med. (1226-3613) 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease yun-2013a-6 LRRK2 phosphorylates Snapin and inhibits interaction of Snapin with SNAP-25. mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yun H.J., Park J., Ho D.H., Kim H., Kim C.H., Oh H., Ga I., Seo H., Chang S., Son I., Seol W. Synapse - Interactions of proteins with an established role in the presynapse. GST-SNAP-25:- a gift from Sheng Z.H. This construct had been entered as Rattine in origin - see Yokoyama CT et al., 1997 PMID: 9278528. The Interactions shown in figure 5 (syntaxin IP) cannot be included due to the lack of specificity of the anti-syntaxin antibody used. Results from the in vitro Kinase assay (Figure 1A) have not been included as the purification (IP only) may not have been sufficient. sonih@wku.ac.kr,wseol@wmcsb.co.kr Exp. Mol. Med. (1226-3613) 2013 Only protein-protein interactions imex curation Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease snapn_human SNARE-associated protein Snapin SNAPIN SNAP25BP SNAPAP Synaptosomal-associated protein 25-binding protein BLOC1S7 Biogenesis of lysosome-related organelles complex 1 subunit 7 protein protein human Homo sapiens Human MAGAGSAAVSGAGTPVAGPTGRDLFAEGLLEFLRPAVQQLDSHVHAVRESQVELREQIDNLATELCRINEDQKVALDLDPYVKKLLNARRRVVLVNNILQNAQERLRRLNHSVAKETARRRAMLDSGIYPPGSPGK lrrk2_human Leucine-rich repeat serine/threonine-protein kinase 2 LRRK2 PARK8 Dardarin protein protein human Homo sapiens Human MASGSCQGCEEDEETLKKLIVRLNNVQEGKQIETLVQILEDLLVFTYSERASKLFQGKNIHVPLLIVLDSYMRVASVQQVGWSLLCKLIEVCPGTMQSLMGPQDVGNDWEVLGVHQLILKMLTVHNASVNLSVIGLKTLDLLLTSGKITLLILDEESDIFMLIFDAMHSFPANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYMILLSALTNFKDEEEIVLHVLHCLHSLAIPCNNVEVLMSGNVRCYNIVVEAMKAFPMSERIQEVSCCLLHRLTLGNFFNILVLNEVHEFVVKAVQQYPENAALQISALSCLALLTETIFLNQDLEEKNENQENDDEGEEDKLFWLEACYKALTWHRKNKHVQEAACWALNNLLMYQNSLHEKIGDEDGHFPAHREVMLSMLMHSSSKEVFQASANALSTLLEQNVNFRKILLSKGIHLNVLELMQKHIHSPEVAESGCKMLNHLFEGSNTSLDIMAAVVPKILTVMKRHETSLPVQLEALRAILHFIVPGMPEESREDTEFHHKLNMVKKQCFKNDIHKLVLAALNRFIGNPGIQKCGLKVISSIVHFPDALEMLSLEGAMDSVLHTLQMYPDDQEIQCLGLSLIGYLITKKNVFIGTGHLLAKILVSSLYRFKDVAEIQTKGFQTILAILKLSASFSKLLVHHSFDLVIFHQMSSNIMEQKDQQFLNLCCKCFAKVAMDDYLKNVMLERACDQNNSIMVECLLLLGADANQAKEGSSLICQVCEKESSPKLVELLLNSGSREQDVRKALTISIGKGDSQIISLLLRRLALDVANNSICLGGFCIGKVEPSWLGPLFPDKTSNLRKQTNIASTLARMVIRYQMKSAVEEGTASGSDGNFSEDVLSKFDEWTFIPDSSMDSVFAQSDDLDSEGSEGSFLVKKKSNSISVGEFYRDAVLQRCSPNLQRHSNSLGPIFDHEDLLKRKRKILSSDDSLRSSKLQSHMRHSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVHLEHLEKLELHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDVSRNDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGNKISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAAMPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQYLPGPAHWKSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFKHIGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQSATVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMSKITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQLVVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRENQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQILTVKVEGCPKHPKGIISRRDVEKFLSKKRKFPKNYMSQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPYMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITVPSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILTTGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKENPQERPTSAQVFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASIWLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALVHLPVEKESWIVSGTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLVGTADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWGGCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAKQNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKELAEKMRRTSVE snp25_rat Synaptosomal-associated protein 25 Snap25 Snap Synaptosomal-associated 25 kDa protein Super protein protein protein rat Rattus norvegicus (Rat) Rat MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG snp25_mouse Synaptosomal-associated protein 25 Synaptosomal-associated 25 kDa protein Super protein Snap25 Snap protein protein mouse Mus musculus Mouse MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG snapn_mouse SNARE-associated protein Snapin Snapin Snap25bp Snapap Synaptosomal-associated protein 25-binding protein Biogenesis of lysosome-related organelles complex 1 subunit 7 Bloc1s7 protein protein mouse Mus musculus Mouse MAAAGSAAVSGAGTPVAGPTGRDLFAEGLLEFLRPAVQQLDSHVHAVRESQVELREQIDNLATELCRINEDQKVALDLDPYVKKLLNARRRVVLVNNILQNAQERLRRLNHSVAKETARRRAMLDSGVYPPGSPSK lrrk2-snapin 1436607 n/a 1436613 enzyme target enzyme target substrate substrate unspecified role unspecified role O95295:p.Thr117Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1436614 enzyme enzyme unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false phosphorylation phosphorylation reaction phosphorylation false false false 1B, 1D lrrk2-1 1436607 n/a 1436614 putative self putative self putative self putative self region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false phosphorylation phosphorylation reaction phosphorylation false true false 1B snapin-lrrk2 1436608 n/a 1436614 unspecified role unspecified role prey prey n_terminal_d_apai_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false roc_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. n/a 1436613 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 2A/B, 3B All tested LRRK2 domain constructs except the Kinase domain construct interacted with Snapin. snap25-snapin 1436608 n/a 1436615 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 1436613 unspecified role unspecified role prey prey O95295:p.Thr117Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 4 A/B Snapin, under conditions which might have resulted in its phosphorylation by LRRK2, showed a considerably decreased interaction with SNAP-25. lrrk2-snapin-1 1436609 n/a 1436613 unspecified role unspecified role prey prey c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1436614 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2C lrrk2-snapin-2 1436610 n/a 1436613 unspecified role unspecified role prey prey n/a 1436614 unspecified role unspecified role bait bait physical association physical association false false false 2D lrrk2-snapin-3 1436611 n/a 1436614 unspecified role unspecified role bait bait n/a 1436613 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false no figure snap25-snapin-1 1436612 n/a 1436616 unspecified role unspecified role bait bait n/a 1436617 unspecified role unspecified role prey prey physical association physical association false false false 4C The i nteraction of SNAP25 with Snapin was weaker when G2019S LRRK2 was present rather than non-mutated LRRK2.