IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ poteryaev-2010-2 Identification of the switch in early-to-late endosome transition. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Poteryaev D., Datta S., Ackema K., Zerial M., Spang A. Synapse - Interactions of proteins with an established role in the presynapse. anne.spang@unibas.ch Cell (0092-8674) 2010 Only protein-protein interactions imex curation poteryaev-2010-3 Identification of the switch in early-to-late endosome transition. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Poteryaev D., Datta S., Ackema K., Zerial M., Spang A. Synapse - Interactions of proteins with an established role in the presynapse. anne.spang@unibas.ch Cell (0092-8674) 2010 Only protein-protein interactions imex curation poteryaev-2010-1 Identification of the switch in early-to-late endosome transition. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Poteryaev D., Datta S., Ackema K., Zerial M., Spang A. Synapse - Interactions of proteins with an established role in the presynapse. anne.spang@unibas.ch Cell (0092-8674) 2010 Only protein-protein interactions imex curation poteryaev-2010-5 Identification of the switch in early-to-late endosome transition. in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay western blot western blot Immuno blot Immuno blot Poteryaev D., Datta S., Ackema K., Zerial M., Spang A. Synapse - Interactions of proteins with an established role in the presynapse. anne.spang@unibas.ch Cell (0092-8674) 2010 Only protein-protein interactions imex curation poteryaev-2010-4 Identification of the switch in early-to-late endosome transition. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging anti tag immunost anti tag immunostaining anti tag immunost Poteryaev D., Datta S., Ackema K., Zerial M., Spang A. Synapse - Interactions of proteins with an established role in the presynapse. Fluorescently labelled proteins visualised by tag fluorescence. anne.spang@unibas.ch Cell (0092-8674) 2010 Only protein-protein interactions imex curation poteryaev-2010-6 Identification of the switch in early-to-late endosome transition. caeel-coelomocyte Caenorhabditis elegans coelomyocyte coelomocyte Coelomocyte Large cells full of vesicles that are situated in the pseudocoelomic space of the worms. Scavenger cells that continuously and nonspecifically endocytose fluid from the pseudocoelom (body cavity). fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Poteryaev D., Datta S., Ackema K., Zerial M., Spang A. Synapse - Interactions of proteins with an established role in the presynapse. anne.spang@unibas.ch Cell (0092-8674) 2010 Only protein-protein interactions imex curation q9bi89_caeel Vacuolar fusion protein MON1 homolog sand-1 F41H10.11 CELE_F41H10.11 protein protein caeel Caenorhabditis elegans MEVEVPGGSEAADTGSEASYKVSQVEIEDVEENLKNADVIFEHLEQLPFQVFILSEFGKPIFVNNDRNEGEIVSLVALICAFVSRCQSWGDSLMTMTSQDNHIQFLHKSPLIFCVVSKYPEQLDQQLEVLFEQICSILSKSQLENVYKKKGDNYDLRKLLRGTDRLIDSSISSWRASPINLVDSSISAIPMNPSDREFLSTTMASCLGAAKLDGALFGIMIARRQIAAMVRFKKYMIHPRDLNIVINLVSDNTLQTDSQNWVPICLPRFNDTGFFYAYISYPWCNKEQDIPVCIVLLSVKRDHFDGLKEVRQQIVTKLENNQKFFVNFAQAMKTPNLYQISQIGSNSESLWSFLYLNHSSKQVCMSASKIPLITRDERWIARSEMRRSSSLSSLHPHLRTLFVRGTKHCLFVWVTDLFSLYCIFGPFVTATIAFQVVEKLLKSLKSHEQRYFIINSTSF q8i100_caeel rabx-5 CELE_Y39A1A.5 Y39A1A.5 protein protein caeel Caenorhabditis elegans MSDKHGEEDKKAKMHLKANDLLCVNGCGFYGTPQWENRCSKCWRAHQNEMKKCQDFAKNRSLLSFDQFQERRKSTTESKSRGIKNLFKTSPIPEGGTSSPTSTSPASTPTRRRELSPDSLEARQQFTDFLVANLSTGMAQEIARSVKNAVNKISEMRMSSDDMSELVMSYYQYLGERIGGHSLFDSPDCKVKVEDVMDQVEKYISTFCYSIFFCANHEEEVADMSLQDRIRSLHWVTAGFLETKMVFKKQTVRDKIDEAISELIEINAKRSAFEKLDCLTKSCKAIFEALKESEASTSADEFLPTLIYVLFRGNPPLIQSNVKFISRFAIPARLMSGEAAYFFTNLSCALEFARNMNHESLQMEKSEFEAYTSGHLAPPLSVINSACNQAIYVLEGTIETITNVAKKAGSLAKNLTNMHGKSDDDLEKMLAVIKETVDFYPTDEYMNMKQSIFAEEKETADILVSLSRQSSESGRGTLTDTQPSTPQPDTSLESPLIAEIQAINLTSSGNQEHVEEA vps16_human Vacuolar protein sorting-associated protein 16 homolog VPS16 protein protein human Homo sapiens Human MDCYTANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWRKEKAASVRPVLDIYSASGMPLASLLWKSGPVVSLGWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLQSAPSCWTVLCQDRVAHILLAVGPDLYLLDHAACSAVTPPGLAPGVSSFLQMAVSFTYRHLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGDAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPAASEEIFKIASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLTQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFPPDSFVHMCQDLRVLNAVRDYHIGIPLTYSQYKQLTIQVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWACYKVQQKDVSDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELETLKDLYNQDDNHQELGSFHIRASYAAEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRLQRRLEDELGGQFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLTALADLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKYEAKKYASRVGPEQKVKALLLVGDVAQAADVAIEHRNEAELSLVLSHCTGATDGATADKIQRARAQAQKK mon1b_human Vacuolar fusion protein MON1 homolog B MON1B HSRG1 KIAA0872 SAND2 HSV-I stimulating-related protein HSV-1 stimulation-related gene 1 protein protein protein human Homo sapiens Human MEVGGDTAAPAPGGAEDLEDTQFPSEEAREGGGVHAVPPDPEDEGLEETGSKDKDQPPSPSPPPQSEALSSTSRLWSPAAPENSPTCSPESSSGGQGGDPSDEEWRSQRKHVFVLSEAGKPIYSRYGSVEALSATMGVMTALVSFVQSAGDAIRAIYAEDHKLVFLQQGPLLLVAMSRTSQSAAQLRGELLAVHAQIVSTLTRASVARIFAHKQNYDLRRLLAGSERTLDRLLDSMEQDPGALLLGAVRCVPLARPLRDALGALLRRCTAPGLALSVLAVGGRLITAAQERNVLAECRLDPADLQLLLDWVGAPAFAAGEAWAPVCLPRFNPDGFFYAYVARLDAMPVCLLLLGTQREAFHAMAACRRLVEDGMHALGAMRALGEAASFSNASSASAPAYSVQAVGAPGLRHFLYKPLDIPDHHRQLPQFTSPELEAPYSREEERQRLSDLYHRLHARLHSTSRPLRLIYHVAEKETLLAWVTSKFELYTCLSPLVTKAGAILVVTKLLRWVKKEEDRLFIRYPPKYSTPPATSTDQAAHNGLFTGL vps18_human Vacuolar protein sorting-associated protein 18 homolog VPS18 KIAA1475 protein protein human Homo sapiens Human MASILDEYENSLSRSAVLQPGCPSVGIPHSGYVNAQLEKEVPIFTKQRIDFTPSERITSLVVSSNQLCMSLGKDTLLRIDLGKANEPNHVELGRKDDAKVHKMFLDHTGSHLLIALSSTEVLYVNRNGQKVRPLARWKGQLVESVGWNKALGTESSTGPILVGTAQGHIFEAELSASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDGRSFVIATTRQRLFQFIGRAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVLRDHFLEKFGPLKHMVKDSSTGQLWAYTERAVFRYHVQREARDVWRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYALTQSYFEEIALKFLEARQEEALAEFLQRKLASLKPAERTQATLLTTWLTELYLSRLGALQGDPEALTLYRETKECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGEVQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGRPDSLLAYLEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASCPLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCATCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAKGSARAKEAEGGAATAGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQRYEEEQLSWL vp33a_human Vacuolar protein sorting-associated protein 33A VPS33A protein protein human Homo sapiens Human MAAHLSYGRVNLNVLREAVRRELREFLDKCAGSKAIVWDEYLTGPFGLIAQYSLLKEHEVEKMFTLKGNRLPAADVKNIIFFVRPRLELMDIIAENVLSEDRRGPTRDFHILFVPRRSLLCEQRLKDLGVLGSFIHREEYSLDLIPFDGDLLSMESEGAFKECYLEGDQTSLYHAAKGLMTLQALYGTIPQIFGKGECARQVANMMIRMKREFTGSQNSIFPVFDNLLLLDRNVDLLTPLATQLTYEGLIDEIYGIQNSYVKLPPEKFAPKKQGDGGKDLPTEAKKLQLNSAEELYAEIRDKNFNAVGSVLSKKAKIISAAFEERHNAKTVGEIKQFVSQLPHMQAARGSLANHTSIAELIKDVTTSEDFFDKLTVEQEFMSGIDTDKVNNYIEDCIAQKHSLIKVLRLVCLQSVCNSGLKQKVLDYYKREILQTYGYEHILTLHNLEKAGLLKPQTGGRNNYPTIRKTLRLWMDDVNEQNPTDISYVYSGYAPLSVRLAQLLSRPGWRSIEEVLRILPGPHFEERQPLPTGLQKKRQPGENRVTLIFFLGGVTFAEIAALRFLSQLEDGGTEYVIATTKLMNGTSWIEALMEKPF vps41_human Vacuolar protein sorting-associated protein 41 homolog VPS41 S53 protein protein human Homo sapiens Human MAEAEEQETGSLEESTDESEEEESEEEPKLKYERLSNGVTEILQKDAASCMTVHDKFLALGTHYGKVYLLDVQGNITQKFDVSPVKINQISLDESGEHMGVCSEDGKVQVFGLYSGEEFHETFDCPIKIIAVHPHFVRSSCKQFVTGGKKLLLFERSWMNRWKSAVLHEGEGNIRSVKWRGHLIAWANNMGVKIFDIISKQRITNVPRDDISLRPDMYPCSLCWKDNVTLIIGWGTSVKVCSVKERHASEMRDLPSRYVEIVSQFETEFYISGLAPLCDQLVVLSYVKEISEKTEREYCARPRLDIIQPLSETCEEISSDALTVRGFQENECRDYHLEYSEGESLFYIVSPRDVVVAKERDQDDHIDWLLEKKKYEEALMAAEISQKNIKRHKILDIGLAYINHLVERGDYDIAARKCQKILGKNAALWEYEVYKFKEIGQLKAISPYLPRGDPVLKPLIYEMILHEFLESDYEGFATLIREWPGDLYNNSVIVQAVRDHLKKDSQNKTLLKTLAELYTYDKNYGNALEIYLTLRHKDVFQLIHKHNLFSSIKDKIVLLMDFDSEKAVDMLLDNEDKISIKKVVEELEDRPELQHVYLHKLFKRDHHKGQRYHEKQISLYAEYDRPNLLPFLRDSTHCPLEKALEICQQRNFVEETVYLLSRMGNSRSALKMIMEELHDVDKAIEFAKEQDDGELWEDLILYSIDKPPFITGLLNNIGTHVDPILLIHRIKEGMEIPNLRDSLVKILQDYNLQILLREGCKKILVADSLSLLKKMHRTQMKGVLVDEENICESCLSPILPSDAAKPFSVVVFHCRHMFHKECLPMPSMNSAAQFCNICSAKNRGPGSAILEMKK mon1a_human Vacuolar fusion protein MON1 homolog A MON1A SAND1 protein protein human Homo sapiens Human MHPGGGPSRAERLELGLGRERPAKAIFLHRRPGEGGGRERCLRCGHVCVRRGPGPREAVPSGRPRPDTLTPPWVRQRAVTGTFCASWTPLRNRRAQRMATDMQRKRSSECLDGTLTPSDGQSMERAESPTPGMAQGMEPGAGQEGAMFVHARSYEDLTESEDGAASGDSHKEGTRGPPPLPTDMRQISQDFSELSTQLTGVARDLQEEMLPGSSEDWLEPPGAVGRPATEPPREGTTEGDEEDATEAWRLHQKHVFVLSEAGKPVYSRYGSEEALSSTMGVMVALVSFLEADKNAIRSIHADGYKVVFVRRSPLVLVAVARTRQSAQELAQELLYIYYQILSLLTGAQLSHIFQQKQNYDLRRLLSGSERITDNLLQLMARDPSFLMGAARCLPLAAAVRDTVSASLQQARARSLVFSILLARNQLVALVRRKDQFLHPIDLHLLFNLISSSSSFREGEAWTPVCLPKFNAAGFFHAHISYLEPDTDLCLLLVSTDREDFFAVSDCRRRFQERLRKRGAHLALREALRTPYYSVAQVGIPDLRHFLYKSKSSGLFTSPEIEAPYTSEEEQERLLGLYQYLHSRAHNASRPLKTIYYTGPNENLLAWVTGAFELYMCYSPLGTKASAVSAIHKLMRWIRKEEDRLFILTPLTY q9xvl8_caeel ccz-1 F58G11.6 CELE_F58G11.6 protein protein caeel Caenorhabditis elegans MESIANPLYFFSNLSLNPPSCSNVVRFIDVLEFFFVAHPLSGRKEGEEHNRVMYFHPKGEQLERQTEITGFAEAVVNFTENFLSTSRREISERISENEDGFDFRTVTTQRTEHVYIRTEDDQFILGVSISKQLSLVSDYPLFQPAIRSILSDAYKMFRMFFGTFSSFIKNVPDDIPKFKERLDFFFSKYIPLLKVHKMPLLDHLGGVEFLRMSGPLYLNVVSLLSELREEFQVIEKIMFLYQDKLLYYQLSKRDLPSLFHYLTHNLLPTTLAPELEHSGRNASKGRYLRGPTDLTTDAPLLGDESLSVVHLHSDQGTEFQEELIGYQMIVYRCLNATVCMFVRNCPANGGLEIRRDSESSQSSRATPSQNGVVSRRLLRNIDQFLETELSQVASKIGDEIGDEKFPEAPDFHYIYFNPSSLSMTSSLSTSPKLPVAASGSNSTLKVPLPPVDVNRLVCDTMSNFVSETEEFGECFVKSSSDWWIVIKKVNSRLLVLIIPPSNYTSSLADVQSKTATIVRSHFEAIFFS vps11_human Vacuolar protein sorting-associated protein 11 homolog VPS11 PP3476 RING finger protein 108 RNF108 protein protein human Homo sapiens Human MAAYLQWRRFVFFDKELVKEPLSNDGAAPGATPASGSAASKFLCLPPGITVCDSGRGSLVFGDMEGQIWFLPRSLQLTGFQAYKLRVTHLYQLKQHNILASVGEDEEGINPLVKIWNLEKRDGGNPLCTRIFPAIPGTEPTVVSCLTVHENLNFMAIGFTDGSVTLNKGDITRDRHSKTQILHKGNYPVTGLAFRQAGKTTHLFVVTTENVQSYIVSGKDYPRVELDTHGCGLRCSALSDPSQDLQFIVAGDECVYLYQPDERGPCFAFEGHKLIAHWFRGYLIIVSRDRKVSPKSEFTSRDSQSSDKQILNIYDLCNKFIAYSTVFEDVVDVLAEWGSLYVLTRDGRVHALQEKDTQTKLEMLFKKNLFEMAINLAKSQHLDSDGLAQIFMQYGDHLYSKGNHDGAVQQYIRTIGKLEPSYVIRKFLDAQRIHNLTAYLQTLHRQSLANADHTTLLLNCYTKLKDSSKLEEFIKKKSESEVHFDVETAIKVLRQAGYYSHALYLAENHAHHEWYLKIQLEDIKNYQEALRYIGKLPFEQAESNMKRYGKILMHHIPEQTTQLLKGLCTDYRPSLEGRSDREAPGCRANSEEFIPIFANNPRELKAFLEHMSEVQPDSPQGIYDTLLELRLQNWAHEKDPQVKEKLHAEAISLLKSGRFCDVFDKALVLCQMHDFQDGVLYLYEQGKLFQQIMHYHMQHEQYRQVISVCERHGEQDPSLWEQALSYFARKEEDCKEYVAAVLKHIENKNLMPPLLVVQTLAHNSTATLSVIRDYLVQKLQKQSQQIAQDELRVRRYREETTRIRQEIQELKASPKIFQKTKCSICNSALELPSVHFLCGHSFHQHCFESYSESDADCPTCLPENRKVMDMIRAQEQKRDLHDQFQHQLRCSNDSFSVIADYFGRGVFNKLTLLTDPPTARLTSSLEAGLQRDLLMHSRRGT p91857_caeel rab-5 CELE_F26H9.6 F26H9.6 protein protein caeel Caenorhabditis elegans MAARNAGTARPGGPNRTCQFKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQTVCLDDATIKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNQESFQKAKNWVKELQRQASPNIVMALAGNKADVANKRTVEYEEANAYAEDNALLFMETSAKTSMNVNDIFMAIAKKLPIGPAQGEPTGTVDMNQPQQQQKGSCCK pi3p 1-phosphatidyl-1D-myo-inositol 3-phosphates small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) pi4p 1-phosphatidyl-1D-myo-inositol 4-phosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) pi5p 1-phosphatidyl-1D-myo-inositol 5-phosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) q23146_caeel rab-7 CELE_W03C9.3 W03C9.3 protein protein caeel Caenorhabditis elegans MSGTRKKALLKVIILGDSGVGKTSLMNQYVNRRFSNQYKATIGADFLTRDVNIDDRTVTLQIWDTAGQERFQSLGVAFYRGADCCVLAFDVTNAASFKSLDSWRDEFLIQASPRDPDHFPFVLLGNKVDLESQRAVSSKRAQSWCQTKGNIPYYEVSAKEALNVEAAFLAIARDALARESQETNDFPEFPDQIRLNPNQQNQQNSGCNC rabx5-sand1 Interaction between C.elegans rabx5 and sand1 909702 n/a 909708 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false in vitro In vitro n/a 909709 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Supp. Fig. 2B Figure legend appears to be incorrect. mon1b-vps16-1 Interaction between MON1B and VPS16A 909702 n/a 909710 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 909711 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 7F mon1b-vps18-2 Interaction between MON1B and VPS18 909702 n/a 909711 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 909712 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig. 7F mon1b-vps33a-1 Interaction between MON1B and VPS33A 909702 n/a 909711 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 909713 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig. 7F mon1b-vps41-1 Interaction between MON1B and VPS33 909702 n/a 909714 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 909711 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 7F mon1a-vps33a-1 Interaction between MON1A and VPS33A 909702 n/a 909715 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 909713 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig. 7F rabx5-sand1-1 Interaction between C.elegans rabx5 and sand1 909703 n/a 909708 unspecified role unspecified role bait bait n/a 909709 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 2A, Supp. Fig. 2C mon1b-ccz1 Interaction between mon1B and ccz1 909703 n/a 909716 unspecified role unspecified role prey prey n/a 909711 unspecified role unspecified role bait bait physical association physical association false false false Fig. 7D mon1b-vps11-1 Interaction between mon1B and vps11 909703 n/a 909717 unspecified role unspecified role prey prey n/a 909711 unspecified role unspecified role bait bait physical association physical association false false false Fig. 7D mon1b-vps18-1 Interaction between mon1B and vps18 909703 n/a 909711 unspecified role unspecified role bait bait n/a 909712 unspecified role unspecified role prey prey physical association physical association false false false Fig. 7D sand1-ccz1-1 Interaction between sand1 and ccz1 909703 n/a 909716 unspecified role unspecified role prey prey n/a 909708 unspecified role unspecified role bait bait physical association physical association false false false Supp. Fig. 2C rabx5-mon1b-1 Interaction between C.elegans rabx5 and human mon1b 909704 n/a 909711 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 909709 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 2B, Supp. Fig. 2E rabx5-rab5 Interaction between C.elegans rabx5 and rab5 909704 n/a 909718 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 909709 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 2B rabx5-mon1a-1 Interaction between C.elegans rabx5 and human mon1a 909704 n/a 909715 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 909709 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Supp. Fig. 2E pi3p-sand1-1 Binding of sand-1 to PI3P 909705 n/a 909708 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 909719 unspecified role unspecified role bait bait direct interaction direct interaction false false false Fig. 6C, Supp. Fig. 5 pi4p-sand1-2 Binding of sand-1 to PI4P 909705 n/a 909708 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 909720 unspecified role unspecified role bait bait direct interaction direct interaction false false false Fig. 6C, Supp. Fig. 5 pi5p-sand1-1 Binding of sand-1 to PI5P 909705 n/a 909721 unspecified role unspecified role bait bait n/a 909708 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 6C, Supp. Fig. 5 rabx5-mon1b-2 Interaction between C.elegans rabx5 and human mon1b 909706 n/a 909709 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 909715 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 909711 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. 4E sand1-rab7 Colocalisation of sand-1 and rab-7 909707 n/a 909722 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 909708 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Figs. 6B, 7A Wortmannin