IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ahuja-2007-3 Cordon-Bleu Is an Actin Nucleation Factor and Controls Neuronal Morphology. in vitro In vitro pull down pull down tag fluorescence tag visualisation by fluorescence tag fluorescence Ahuja R., Pinyol R., Reichenbach N., Custer L., Klingensmith J., Kessels MM., Qualmann B. Synapse - Interactions of proteins with an established role in the presynapse. Interactions with actin identified only by antibody staining could not be added due to non-specificity of Abs for different actin gene products Beads coated with recombinant proteins were incubated with rat brain extracts supplemented with an energy regenerating mix and fluorescently labeled rabbit skeletal G-actin. Accumulation of labelled actim polmerizing on the bead surface was taken as a measure of interaction with the bait protein Source of Syndactin i (Pacsin1) taken form PMID: 9950691 qualmann@ifn-magdeburg.de Mouse brain cDNA Cell (0092-8674) 2007 Only protein-protein interactions 6 imex curation ahuja-2007-2 Cordon-Bleu Is an Actin Nucleation Factor and Controls Neuronal Morphology. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Ahuja R., Pinyol R., Reichenbach N., Custer L., Klingensmith J., Kessels MM., Qualmann B. Synapse - Interactions of proteins with an established role in the presynapse. Source of Syndactin i (Pacsin1) taken form PMID: 9950691 qualmann@ifn-magdeburg.de Mouse brain cDNA Cell (0092-8674) 2007 Only protein-protein interactions 6 imex curation ahuja-2007-1 Cordon-Bleu Is an Actin Nucleation Factor and Controls Neuronal Morphology. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Ahuja R., Pinyol R., Reichenbach N., Custer L., Klingensmith J., Kessels MM., Qualmann B. Synapse - Interactions of proteins with an established role in the presynapse. Source of Syndactin i (Pacsin1) taken form PMID: 9950691 qualmann@ifn-magdeburg.de Mouse brain cDNA Cell (0092-8674) 2007 Only protein-protein interactions 6 imex curation acts_rabit Actin, alpha skeletal muscle ACTA1 ACTA Alpha-actin-1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF cobl_mouse Protein cordon-bleu Cobl Kiaa0633 protein protein mouse Mus musculus Mouse MDAPRALAAKPPTGRKMKARAPPPPGKPAAQNVHSEQKLPHDATLGSQQSLVYMKEALQNSTLDITVVLPSGLEKQSVVSGSHAMMDLLVELCLQNHLNPSHHVLEIWSSETQQPLSFKPNTLIGSLNVHTVLLKEKVPEERVKPGLTKAPEKSVRLVVNYLRTQKAVVRVSPEVPLQNILPVICAKCEVNPEHVILLRDNVAGEELELSKSLNELGIKELYAWDNRREMFRKSSLGNDETDKEKKKFLGFFKANKRSNSKAEHLGLSGADSDEDPAKSASGGDLNGCVTTPNSPSLHSRSLTLGPSLSLGNISGVSMKSDMKKRRAPPPPSPKLLGQDKVSEKASLSSQADLQKKKRRAPAPPPPQQPPPSPVVPNRKEDKEENRKSTVGVGRQVPQKPPRGTARGPPQLVLPPPPPYPPPDTDVTEPVTFPGEGAGSETSELRPKLSLPLGPGSHCSMGGVSQVPAESEETASEDTTEDSGVMSSPSDAISLDSQQDSMRSKDKWSTDQEDGSDQDLAGTPELGPQKSPSWGKSGSGSSILRTEKATMPTNDDEDLFITGHLHQTLAELDEDLEGMEENYETDTSSLTNSVNGVSNHSLQEAIIPDSGVDDIPVTFIGEVSDEPFDSGLFSSRCNNATTFNTGSIASQRSHLSPSQTEHSQPFVRTSRKEPDPSPPSQDNRKRNQPTLANTSENENPVETDPTVTSLVSKLLIDDPKAKDKGKVHGSSHSEKTQAGHGINSLRVNPRDGKDESSNSAPPPWSHHGQALGGSYGLKYGLTTYKIVPPKSEMRCYDRDVSLSTGAIKIDELGNLVSPHMNGSRTISPPSAVVETDTPPIGKVKEFWRRNSMEKYLNGPAECTIKRAPSTTITATPEKPQQDNGMKAAFTVTTPQQQPASQEYGAHLEEERSRPQSAVSCSVKVPASNPTDITFLKPQRRTSSQYVASAIAKKMGPPKVHADVVRPHKATTEQCHEEAKLARSPPTRKDDAAPNLHSEARQHEHGTNQSSVCLPSNPGVQLPAGGHPKVEVNSTYGKSSTQDYPAAVHRNSYFLPGRSSHRDRVSVGQSCGFNEKQTTSNQKANSTSNFSQALDKAHPPPLLLAEARDSGRILMNGSARTPGNCEPPHSPKESTLTSYIILQTEEKPSSLSTDGQDADDTLPSSIFGPKKKFKPVIQRPLPKDVSLHSALMEAIHSSGGREKLRKTAEQTSEGRPKKPSYVEAESERSALLAAIRGHSGTLSLRKVSSLASEELQSFRNAALGAPGLDKPQQEDLGLPPPPALPPPPAPAPQAPSASVTVSRFSTGTPSNSVNARQALMDAIRSGTGAARLRKVPLLV dbnl_mouse Drebrin-like protein Dbnl Abp1 Sh3p7 SH3 domain-containing protein 7 Actin-binding protein 1 protein protein mouse Mus musculus Mouse MAVNLSRNGPALQEAYVRVVTEKSPTDWALFTYEGNSNDIRVAGTGEGGLEELVEELNSGKVMYAFCRVKDPNSGLPKFVLINWTGEGVNDVRKGACANHVSTMANFLKGAHVTINARAEEDVEPECIMEKVAKASGANYSFHKESTSFQDVGPQAPVGSVYQKTNAISEIKRVGKDNFWAKAEKEEENRRLEEKRRAEEERQRLEEERRERELQEAARREQRYQEQHRSAGAPSPSSRTGEPEQEAVSRTRQEWESAGQQAPHPREIFKQKERAMSTTSVTSSQPGKLRSPFLQKQLTQPETSYGREPTAPVSRPAAGVCEEPAPSTLSSAQTEEEPTYEVPPEQDTLYEEPPLVQQQGAGSEHIDNYMQSQGFSGQGLCARALYDYQAADDTEISFDPENLITGIEVIDEGWWRGYGPDGHFGMFPANYVELIE pacn1_rat Protein kinase C and casein kinase substrate in neurons protein 1 Pacsin1 Synaptic, dynamin-associated protein I Syndapin-I Dynamin proline-rich domain-interacting protein Syndapin-1 protein protein rat Rattus norvegicus (Rat) Rat MSGPYDEASEEITDSFWEVGNYKRTVKRIDDGHRLCNDLMSCVQERAKIEKAYAQQLTDWAKRWRQLIEKGPQYGSLERAWGAMMTEADKVSELHQEVKNSLLNEDLEKVKNWQKDAYHKQIMGGFKETKEAEDGFRKAQKPWAKKMKELEAAKKAYHLACKEEKLAMTREMNSKTEQSVTPEQQKKLVDKVDKCRQDVQKTQEKYEKVLEDVGKTTPQYMEGMEQVFEQCQQFEEKRLVFLKEVLLDIKRHLNLAENSSYIHVYRELEQAIRGADAQEDLRWFRSTSGPGMPMNWPQFEEWNPDLPHTAAKKEKQPKKAEGAALSNATGAVESTSQAGDRGSVSSYDRGQAYATEWSDDESGNPFGGNEANGGANPFEDDAKGVRVRALYDYDGQEQDELSFKAGDELTKLGEEDEQGWCRGRLDSGQLGLYPANYVEAI cobl-acta1-1 Interaction between murine Cobl and rabit skeletal actin identified by pulldown 677092 N/A 677095 unspecified role unspecified role prey prey region fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 677096 unspecified role unspecified role bait bait wh2-1 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false wh2-2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false wh2-3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figures 2D-2G, 2K-2M Determination of the G-actin dissociation constants for the three WH2 domains by measuring the fluorescence of NBD-labeled actin incubated with different concentrations of the GST-WH2 domain fusion proteins: WH21-1 = 39 nM, WH2-2 = 40 nM , WH2-3 = 432 nM dbnl-acta1-1 Interaction between murine Dbnl and rabit skeletal actin identified by pulldown 677092 N/A 677097 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 677095 unspecified role unspecified role prey prey tag fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figures 1B-1D, Supp. Figs 1A-1F pacsin1-acta1-1 Interaction between rat Pacsin1 and rabbit skeletal actin identified by pulldown 677092 N/A 677095 unspecified role unspecified role prey prey region fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 677098 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Figures 1B-1D, Supp. Figs 1A-1F cobl-pacsin1-2 Interaction between murine Cobl and rat Pacsin1 identified by Y2H 677093 N/A 677096 unspecified role unspecified role prey prey N/A 677098 unspecified role unspecified role bait bait physical association physical association false false false Figure 1E, Supp Fig S1G cobl-dbnl-1 Interaction between Cobl and Dbnl identified by Y2H 677093 N/A 677097 unspecified role unspecified role prey prey N/A 677096 unspecified role unspecified role bait bait physical association physical association false false false Figure 1F, Supp Fig S1H cobl-pacsin1-1 Interaction between murine Cobl and rat Pacsin1 identified by Y2H 677094 N/A 677096 unspecified role unspecified role prey prey N/A 677098 unspecified role unspecified role bait bait physical association physical association false false false