IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ penengo-2006-1 Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Penengo L., Mapelli M., Murachelli AG., Confalonieri S., Magri L., Musacchio A., Di Fiore PP., Polo S., Schneider TR. Synapse - Interactions of proteins with an established role in the presynapse. 1, S1, 6A, 6B, 6E pierpaolo.difiore@ifom-ieo-campus.it,simona.polo@ifom-ieo-campus.it,thomas.schneider@ifom-ieo-campus.it Cell (0092-8674) 2006 Only protein-protein interactions 14 imex curation penengo-2006-5 Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Penengo L., Mapelli M., Murachelli AG., Confalonieri S., Magri L., Musacchio A., Di Fiore PP., Polo S., Schneider TR. Synapse - Interactions of proteins with an established role in the presynapse. 2 pierpaolo.difiore@ifom-ieo-campus.it,simona.polo@ifom-ieo-campus.it,thomas.schneider@ifom-ieo-campus.it Cell (0092-8674) 2006 Only protein-protein interactions 14 imex curation penengo-2006-2 Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin. in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Penengo L., Mapelli M., Murachelli AG., Confalonieri S., Magri L., Musacchio A., Di Fiore PP., Polo S., Schneider TR. Synapse - Interactions of proteins with an established role in the presynapse. 6C, S6 pierpaolo.difiore@ifom-ieo-campus.it,simona.polo@ifom-ieo-campus.it,thomas.schneider@ifom-ieo-campus.it Cell (0092-8674) 2006 Only protein-protein interactions 14 imex curation penengo-2006-3 Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 pull down pull down western blot western blot Immuno blot Immuno blot Penengo L., Mapelli M., Murachelli AG., Confalonieri S., Magri L., Musacchio A., Di Fiore PP., Polo S., Schneider TR. Synapse - Interactions of proteins with an established role in the presynapse. 1E pierpaolo.difiore@ifom-ieo-campus.it,simona.polo@ifom-ieo-campus.it,thomas.schneider@ifom-ieo-campus.it Cell (0092-8674) 2006 Only protein-protein interactions 14 imex curation penengo-2006-4 Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Penengo L., Mapelli M., Murachelli AG., Confalonieri S., Magri L., Musacchio A., Di Fiore PP., Polo S., Schneider TR. Synapse - Interactions of proteins with an established role in the presynapse. 3, 4 pierpaolo.difiore@ifom-ieo-campus.it,simona.polo@ifom-ieo-campus.it,thomas.schneider@ifom-ieo-campus.it Cell (0092-8674) 2006 Only protein-protein interactions 14 imex curation penengo-2006-6 Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Penengo L., Mapelli M., Murachelli AG., Confalonieri S., Magri L., Musacchio A., Di Fiore PP., Polo S., Schneider TR. Synapse - Interactions of proteins with an established role in the presynapse. 2D, 2E, 2F pierpaolo.difiore@ifom-ieo-campus.it,simona.polo@ifom-ieo-campus.it,thomas.schneider@ifom-ieo-campus.it Cell (0092-8674) 2006 Only protein-protein interactions 14 imex curation penengo-2006-7 Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Penengo L., Mapelli M., Murachelli AG., Confalonieri S., Magri L., Musacchio A., Di Fiore PP., Polo S., Schneider TR. Synapse - Interactions of proteins with an established role in the presynapse. HeLa cells, transfected with GFP-Rabex-5, were incubated (1 hr, on ice) with the 13A9 anti-EGFR antibody. After wash and addition of EGF (100 ng/ml), cells were shifted to 37�C after 0, 2, 8 and 15 hours. 2C, S4 pierpaolo.difiore@ifom-ieo-campus.it,simona.polo@ifom-ieo-campus.it,thomas.schneider@ifom-ieo-campus.it Cell (0092-8674) 2006 Only protein-protein interactions 14 imex curation penengo-2006-8 Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Penengo L., Mapelli M., Murachelli AG., Confalonieri S., Magri L., Musacchio A., Di Fiore PP., Polo S., Schneider TR. Synapse - Interactions of proteins with an established role in the presynapse. 6D pierpaolo.difiore@ifom-ieo-campus.it,simona.polo@ifom-ieo-campus.it,thomas.schneider@ifom-ieo-campus.it Cell (0092-8674) 2006 Only protein-protein interactions 14 imex curation rabx5_human Rab5 GDP/GTP exchange factor Rabex-5 Rabaptin-5-associated exchange factor for Rab5 RAP1 RABGEF1 RABEX5 protein protein human Homo sapiens Human MSLKSERRGIHVDQSDLLCKKGCGYYGNPAWQGFCSKCWREEYHKARQKQIQEDWELAERLQREEEEAFASSQSSQGAQSLTFSKFEEKKTNEKTRKVTTVKKFFSASSRVGSKKEIQEAKAPSPSINRQTSIETDRVSKEFIEFLKTFHKTGQEIYKQTKLFLEGMHYKRDLSIEEQSECAQDFYHNVAERMQTRGKVPPERVEKIMDQIEKYIMTRLYKYVFCPETTDDEKKDLAIQKRIRALRWVTPQMLCVPVNEDIPEVSDMVVKAITDIIEMDSKRVPRDKLACITKCSKHIFNAIKITKNEPASADDFLPTLIYIVLKGNPPRLQSNIQYITRFCNPSRLMTGEDGYYFTNLCCAVAFIEKLDAQSLNLSQEDFDRYMSGQTSPRKQEAESWSPDACLGVKQMYKNLDLLSQLNERQERIMNEAKKLEKDLIDWTDGIAREVQDIVEKYPLEIKPPNQPLAAIDSENVENDKLPPPLQPQVYAG ubb_human Polyubiquitin-B UBB protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGC rn168_human E3 ubiquitin-protein ligase RNF168 RNF168 RING-type E3 ubiquitin transferase RNF168 RING finger protein 168 protein protein human Homo sapiens Human MALPKDAIPSLSECQCGICMEILVEPVTLPCNHTLCKPCFQSTVEKASLCCPFCRRRVSSWTRYHTRRNSLVNVELWTIIQKHYPRECKLRASGQESEEVADDYQPVRLLSKPGELRREYEEEISKVAAERRASEEEENKASEEYIQRLLAEEEEEEKRQAEKRRRAMEEQLKSDEELARKLSIDINNFCEGSISASPLNSRKSDPVTPKSEKKSKNKQRNTGDIQKYLTPKSQFGSASHSEAVQEVRKDSVSKDIDSSDRKSPTGQDTEIEDMPTLSPQISLGVGEQGADSSIESPMPWLCACGAEWYHEGNVKTRPSNHGKELCVLSHERPKTRVPYSKETAVMPCGRTESGCAPTSGVTQTNGNNTGETENEESCLLISKEISKRKNQESSFEAVKDPCFSAKRRKVSPESSPDQEETEINFTQKLIDLEHLLFERHKQEEQDRLLALQLQKEVDKEQMVPNRQKGSPDEYHLRATSSPPDKVLNGQRKNPKDGNFKRQTHTKHPTPERGSRDKNRQVSLKMQLKQSVNRRKMPNSTRDHCKVSKSAHSLQPSISQKSVFQMFQRCTK myo6_human Unconventional myosin-VI MYO6 KIAA0389 Unconventional myosin-6 protein protein human Homo sapiens Human MEDGKPVWAPHPTDGFQMGNIVDIGPDSLTIEPLNQKGKTFLALINQVFPAEEDSKKDVEDNCSLMYLNEATLLHNIKVRYSKDRIYTYVANILIAVNPYFDIPKIYSSEAIKSYQGKSLGTRPPHVFAIADKAFRDMKVLKMSQSIIVSGESGAGKTENTKFVLRYLTESYGTGQDIDDRIVEANPLLEAFGNAKTVRNNNSSRFGKFVEIHFNEKSSVVGGFVSHYLLEKSRICVQGKEERNYHIFYRLCAGASEDIREKLHLSSPDNFRYLNRGCTRYFANKETDKQILQNRKSPEYLKAGSMKDPLLDDHGDFIRMCTAMKKIGLDDEEKLDLFRVVAGVLHLGNIDFEEAGSTSGGCNLKNKSAQSLEYCAELLGLDQDDLRVSLTTRVMLTTAGGTKGTVIKVPLKVEQANNARDALAKTVYSHLFDHVVNRVNQCFPFETSSYFIGVLDIAGFEYFEHNSFEQFCINYCNEKLQQFFNERILKEEQELYQKEGLGVNEVHYVDNQDCIDLIEAKLVGILDILDEENRLPQPSDQHFTSAVHQKHKDHFRLTIPRKSKLAVHRNIRDDEGFIIRHFAGAVCYETTQFVEKNNDALHMSLESLICESRDKFIRELFESSTNNNKDTKQKAGKLSFISVGNKFKTQLNLLLDKLRSTGASFIRCIKPNLKMTSHHFEGAQILSQLQCSGMVSVLDLMQGGYPSRASFHELYNMYKKYMPDKLARLDPRLFCKALFKALGLNENDYKFGLTKVFFRPGKFAEFDQIMKSDPDHLAELVKRVNHWLTCSRWKKVQWCSLSVIKLKNKIKYRAEACIKMQKTIRMWLCKRRHKPRIDGLVKVGTLKKRLDKFNEVVSVLKDGKPEMNKQIKNLEISIDTLMAKIKSTMMTQEQIQKEYDALVKSSEELLSALQKKKQQEEEAERLRRIQEEMEKERKRREEDEKRRRKEEEERRMKLEMEAKRKQEEEERKKREDDEKRIQAEVEAQLARQKEEESQQQAVLEQERRDRELALRIAQSEAELISDEAQADLALRRSLDSYPVSKNDGTRPKMTPEQMAKEMSEFLSRGPAVLATKAAAGTKKYDLSKWKYAELRDTINTSCDIELLAACREEFHRRLKVYHAWKSKNKKRNTETEQRAPKSVTDYDFAPFLNNSPQQNPAAQIPARQREIEMNRQQRFFRIPFIRPADQYKDPQSKKKGWWYAHFDGPWIARQMELHPDKPPILLVAGKDDMEMCELNLEETGLTRKRGAEILPRQFEEIWERCGGIQYLQNAIESRQARPTYATAMLQSLLK eps15_human Epidermal growth factor receptor substrate 15 EPS15 AF1P Protein AF-1p protein protein human Homo sapiens Human MAAAAQLSLTQLSSGNPVYEKYYRQVDTGNTGRVLASDAAAFLKKSGLPDLILGKIWDLADTDGKGILNKQEFFVALRLVACAQNGLEVSLSSLNLAVPPPRFHDTSSPLLISGTSAAELPWAVKPEDKAKYDAIFDSLSPVNGFLSGDKVKPVLLNSKLPVDILGRVWELSDIDHDGMLDRDEFAVAMFLVYCALEKEPVPMSLPPALVPPSKRKTWVVSPAEKAKYDEIFLKTDKDMDGFVSGLEVREIFLKTGLPSTLLAHIWSLCDTKDCGKLSKDQFALAFHLISQKLIKGIDPPHVLTPEMIPPSDRASLQKNIIGSSPVADFSAIKELDTLNNEIVDLQREKNNVEQDLKEKEDTIKQRTSEVQDLQDEVQRENTNLQKLQAQKQQVQELLDELDEQKAQLEEQLKEVRKKCAEEAQLISSLKAELTSQESQISTYEEELAKAREELSRLQQETAELEESVESGKAQLEPLQQHLQDSQQEISSMQMKLMEMKDLENHNSQLNWCSSPHSILVNGATDYCSLSTSSSETANLNEHVEGQSNLESEPIHQESPARSSPELLPSGVTDENEVTTAVTEKVCSELDNNRHSKEEDPFNVDSSSLTGPVADTNLDFFQSDPFVGSDPFKDDPFGKIDPFGGDPFKGSDPFASDCFFRQSTDPFATSSTDPFSAANNSSITSVETLKHNDPFAPGGTVVAASDSATDPFASVFGNESFGGGFADFSTLSKVNNEDPFRSATSSSVSNVVITKNVFEETSVKSEDEPPALPPKIGTPTRPCPLPPGKRSINKLDSPDPFKLNDPFQPFPGNDSPKEKDPEIFCDPFTSATTTTNKEADPSNFANFSAYPSEEDMIEWAKRESEREEEQRLARLNQQEQEDLELAIALSKSEISEA egfr_human Epidermal growth factor receptor ERBB1 EGFR Receptor tyrosine-protein kinase erbB-1 Proto-oncogene c-ErbB-1 ERBB HER1 protein protein human Homo sapiens Human MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA ubiq_bovin Ubiquitin RPS27A UBA80 UBCEP1 UBA52 UBCEP2 UBB UBC protein protein bovin Bos taurus (Bovine) Bovine MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG rabgef1-ub 362108 N/A 362116 unspecified role unspecified role bait bait miu region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9UJ41:p.Ala58Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 362117 unspecified role unspecified role prey prey chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Synthetic PolyUb2-7 linked by K48 was used. The authors have identified Rabex-5 regions AA 2-49 and AA48-74 as two distinct binding sites. A decrease in binding of about 50% was observed in A58G mutant of the complete protein. However, this mutation in the AA 18-74 fragment totally abolished the binding of this region to Ubiquitin. rnf168-ub 362108 N/A 362117 unspecified role unspecified role prey prey chemical synthesis Chemical synthesis (Chemical synthesis) N/A 362118 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false miu1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q8IYW5:p.Ala179Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false miu2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q8IYW5:p.Ala450Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Synthetic PolyUb2-7 linked by K48 and K63 was used. The decrease in binding was more visible with K63. The mutations in the individual MIU domians abolished the binding of the domain completely. myo6-ub 362108 N/A 362119 unspecified role unspecified role bait bait Q9UM54:p.Ala1013Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 362117 unspecified role unspecified role prey prey chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Synthetic PolyUb2-7 linked by K48 and K63 was used. The decrease in binding was more severe with K63 linked Ub. The mutations in the MIU domain abolished the binding of the protein. rabgef1-ub-4 362108 N/A 362116 unspecified role unspecified role bait bait ruz region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9UJ41:p.Tyr25Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 362117 unspecified role unspecified role prey prey P0CG47:p.Asp58Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false polyUB is chemically synthesised along the lines of 'Synthetic PolyUb2-7 linked by K48 was used rabgef1-ub-5 362108 N/A 362117 unspecified role unspecified role prey prey P0CG47:p.Ile44Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 362116 unspecified role unspecified role bait bait miu region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9UJ41:p.Ala58Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false polyUB is chemically synthesised along the lines of 'Synthetic PolyUb2-7 linked by K48 was used rabgef1-ub-6 362108 N/A 362116 unspecified role unspecified role prey prey ruz domain sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 362116 unspecified role unspecified role bait bait miu region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 362117 unspecified role unspecified role prey prey chemical synthesis Chemical synthesis (Chemical synthesis) association association false false false rabgef1-egfr 362109 N/A 362120 unspecified role unspecified role prey prey N/A 362121 unspecified role unspecified role bait bait N/A 362116 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Interaction was observed only on EGF stimutation. rabgef1-egfr-1 362109 N/A 362116 unspecified role unspecified role prey prey N/A 362121 unspecified role unspecified role bait bait physical association physical association false false false Interaction was observed only on EGF stimutation. rabgef1-ub-1 362110 N/A 362116 unspecified role unspecified role neutral component neutral component miu region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ruz region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 362122 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false MIU AA(48-74) Ka 3.5E-6 Kd 28.7E-6, RUZ AA(1-49) Ka 8.3E-6 Kd 12.0E-6, RUZ-MIU AA(1-74) Ka 15.7E-6 Kd 6.4E-6, RUZ-MIUA58G Ka 5.3E-6 Kd 18.8E-6, RUZY25F-MIU AA(1-74) Ka 15.9E-6 Kd 6.3E-6. rabgef1-ub-2 362111 N/A 362117 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 362116 unspecified role unspecified role bait bait miu region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9UJ41:p.Ala58Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Synthetic PolyUb2-7 linked by K48 was used rabgef1-ub-3 362112 N/A 362122 unspecified role unspecified role neutral component neutral component asp58 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false asp58 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tyr26 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false arg54 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false glu51 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ser57 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 362116 unspecified role unspecified role neutral component neutral component miu region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ser-174 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false cys-173 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tyr-163 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false asn-166 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 362186 362187 362193 362185 362194 362188 362195 362189 362196 362190 direct interaction direct interaction false false false The binding between the two molecules is further strengthened by stacking interactions between the side chains of Tyr25Rbx and Arg54Ub. 2.4 and 2.1 A Form I is hexagonal and contains one molecule of RUZ-MIU and one molecule of Ub per asymmetric unit, while form II is triclinic with six copies of each molecule in the unit cell. Within each RUZ-MIU molecule, the RUZ and MIU domains contact two different molecules of Ub conversely, every molecule of Ub contacts two molecules of RUZ-MIU again using two disjoint surfaces. rabgef1-egfr-2 362113 N/A 362121 unspecified role unspecified role prey prey ubiquitin xlnk-n6lys-1gly N6-glycyl-L-lysine (2S)-2-amino-6-[(aminoacetyl)amino]hexanoic acid N6-(glycyl)-L-lysine N6-glycyllysine XLNK-N6Lys-1Gly CROSSLNK Glycyl lysine isopeptide (Gly-Lys) (interchain with K-...) CROSSLNK Glycyl lysine isopeptide (Lys-Gly) (interchain with G-...) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 362116 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9UJ41:p.Ala58Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Interaction was observed only on EGF stimutation. rabgef1-egfr-3 362114 N/A 362121 unspecified role unspecified role neutral component neutral component N/A 362116 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Interaction was observed only on EGF stimutation. rabgef1-ub-7 362115 N/A 362117 unspecified role unspecified role neutral component neutral component P0CG47:p.Asp58Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 N/A 362116 unspecified role unspecified role neutral component neutral component ruz miu region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 362117 unspecified role unspecified role neutral component neutral component P0CG47:p.Ile44Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 2.0 physical association physical association false false false