IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ wang-2006-4 A novel family of adhesion-like molecules that interacts with the NMDA receptor. rat-brain Rattus norvegicus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang CY., Chang K., Petralia RS., Wang YX., Seabold GK., Wenthold RJ. Synapse - Interactions of proteins with an established role in the presynapse. Detergent extracts of crude synaptosomal fractions from 2 week old rat brains were used in this study. Figure 2 (B). Figure 7 (A). wenthold@nidcd.nih.gov Mouse brain cDNA library Antibodies for N-terminal SALM1 (amino acids 384-397) (mouse) and C-terminal (amino acids 684-699) (mouse) were used in this study. J. Neurosci. (0270-6474) 2006 Only protein-protein interactions imex curation wang-2006-3 A novel family of adhesion-like molecules that interacts with the NMDA receptor. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang CY., Chang K., Petralia RS., Wang YX., Seabold GK., Wenthold RJ. Synapse - Interactions of proteins with an established role in the presynapse. Epitope tagged and none epitope tagged expression constructs were co-transfected into HEK293 cell lines in this study. Figure 2 (A). Figure 7 (B)(E) and (F). wenthold@nidcd.nih.gov Mouse brain cDNA library J. Neurosci. (0270-6474) 2006 Only protein-protein interactions imex curation wang-2006-2 A novel family of adhesion-like molecules that interacts with the NMDA receptor. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wang CY., Chang K., Petralia RS., Wang YX., Seabold GK., Wenthold RJ. Synapse - Interactions of proteins with an established role in the presynapse. Epitope tagged and none epitope tagged expression constructs were co-transfected into HEK293 cell lines in this study. Figure 2 (A). Figure 7 (E) and (F). wenthold@nidcd.nih.gov Mouse brain cDNA library J. Neurosci. (0270-6474) 2006 Only protein-protein interactions imex curation wang-2006-6 A novel family of adhesion-like molecules that interacts with the NMDA receptor. rat-hippocampus Rattus norvegicus hippocampal neurons hippocampus Hippocampus [Brain hippocampus] fluorescence imaging fluorescence microscopy fluorescence imaging antibody detection identification by antibody antibody detection Wang CY., Chang K., Petralia RS., Wang YX., Seabold GK., Wenthold RJ. Synapse - Interactions of proteins with an established role in the presynapse. NR2A staining intensity, number of NR2A puncta and percentage area for SALM1 and NR2A colocalisation was measured using MetaMorph software. In some instances 30 micro meter dendritic segments were analysed and data obtained from dendrites of 10 neurons. Figure 4 (B). Figure 5. Figure 6. wenthold@nidcd.nih.gov Mouse brain cDNA library Antibody for SALM1 was raised against mouse SALM1 protein. J. Neurosci. (0270-6474) 2006 Only protein-protein interactions imex curation wang-2006-1 A novel family of adhesion-like molecules that interacts with the NMDA receptor. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Wang CY., Chang K., Petralia RS., Wang YX., Seabold GK., Wenthold RJ. Synapse - Interactions of proteins with an established role in the presynapse. wenthold@nidcd.nih.gov Mouse brain cDNA library J. Neurosci. (0270-6474) 2006 Only protein-protein interactions imex curation wang-2006-5 A novel family of adhesion-like molecules that interacts with the NMDA receptor. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Wang CY., Chang K., Petralia RS., Wang YX., Seabold GK., Wenthold RJ. Synapse - Interactions of proteins with an established role in the presynapse. Figure 2 (C). wenthold@nidcd.nih.gov Mouse brain cDNA library Antibodies used in this study were raised against SALM1 (mouse). J. Neurosci. (0270-6474) 2006 Only protein-protein interactions imex curation dlg4_rat Disks large homolog 4 Dlg4 Dlgh4 Postsynaptic density protein 95 Synapse-associated protein 90 Psd95 protein protein rat Rattus norvegicus (Rat) Rat MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKVMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVIDAGDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL lrfn2_rat Leucine-rich repeat and fibronectin type-III domain-containing protein 2 Lrfn2 Salm1 protein protein rat Rattus norvegicus (Rat) Rat METLLGGLLAFGMAFAVVDACPKYCVCQNLSESLGTLCPSKGLLFVPPDIDRRTVELRLGGNFIIHIGRQDFANMTGLVDLTLSRNTISHIQPFSFLDLESLRSLHLDSNRLPSLGEDTLRGLVNLQHLIVNNNQLGGIADDAFEDFLLTLEDLDLSYNNLHGLPWDSVRRMVNLHQLSLDHNLLDHIAEGTFADLQKLARLDLTSNRLQKLPPDPIFARSQASLLTATPFAPPLSFSFGGNPLHCNCELLWLRRLERDDDLKTCGSPGGLKGRYFWHIREEEFVCEPPLITQHTHKLLVLEGQAATLKCKAIGDPSPLIHWVAPDDRLVGNSSRTAVYDNGTLDILITTSQDSGPFTCIAANAAGEATATVEVSIVQLPHLSNSTSRMAPPKSRLSDITGSSKTSRGGGGSGAGEPPKSTPERAVLVSDVTTTSALVKWSVSKSAPRVKMYQLQYNCSDDEVLIYRMIPASNKAFVVNNLVSGTGYDLCVLAMWDDTATTLTATNIVGCAQFFTKADYPQCQSMHSQILGGTMILVIGGIIVATLLVFIVILMVRYKVCNHDAPGKMAAATVSNVYSQTNGAQPPPLGGMPVGQLPQAPPKVVVRNELMDFSTSLARACDSSSSSSLGSGEAAGLSRGPWRLPPPAPRPKPSLDRLMGAFASLDLKSQRKEELLDSRTPAGRGAGTSARGHHSDREPLLGPPATRARSLLPLPLEGKAKRSHSFDMGDFAAAAAAAPGGYSPPRRVSNIWTKRSLSVNGMLLPFEENDLVGARGTFGSSEWVMESTV dlg1_rat Disks large homolog 1 Dlg1 Synapse-associated protein 97 Dlgh1 protein protein rat Rattus norvegicus (Rat) Rat MPVRKQDTQRALHLLEEYRSKLSQTEDRQLRSSIERVISIFQSNLFQALIDIQEFYEVTLLDNPKCVDHSKQCEPVQPGNPWESGSLSSAAVTSESLPGGLSPPVEKYRYQDEEVLPSERISPQVPNEVLGPELVHVSEKSLSEIENVHGFVSHSHISPIKPTEAVPPSSPIVPVTPALPVPAESPVVLPSTPQANPPPVLVNTDSLETPTYVNGTDADYEYEEITLERGNSGLGFSIAGGTDNPHIGDDSSIFITKIITGGAAAQDGRLRVNDCILRVNEADVRDVTHSKAVEALKEAGSIVRLYVKRRKAFRKNHEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGKLQIGDKLLAVNSVCLEEVTHEEAVTALKNTSDFVYLKAAKPTSMYINDGYAPPDITNSSSQSVDNHVSPSSYLGQTPASPARYSPISKAVLGDDEITREPRKVVLHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDRIISVNSVDLRAASHEQAAAALKNAGQAVTIVAQYRPEEYSRFEAKIHDLRETMMNSSVSSGSGSLRTSQKRSLYVRALFDYDKTKDSGLPSQGLNFKFGDILHVINASDDEWWQARQVTPDGESDEVGVIPSKRRVEKKERARLKTVKFNSKTRGDKGEIPDDMGSKGLKHVTSNASDSESSYHEYGCSKGGQEEYVLSYEPVNQQEVNYTRPVIILGPMKDRVNDDLISEFPDKFGSCVPHTTRPKRDYEVDGRDYHFVTSREQMEKDIQEHKFIEAGQYNNHLYGTSVQSVRAVAEKGKHCILDVSGNAIKRLQIAQLYPISIFIKPKSMENIMEMNKRLTDEQARKTFERAVRLEQEFTEHFTAIVQGDTLEDIYNQVKQIIEEQSGPYIWVPAKEKL dlg3_rat Disks large homolog 3 Dlg3 Dlgh3 Synapse-associated protein 102 PSD-95/SAP90-related protein 1 protein protein rat Rattus norvegicus (Rat) Rat MHKHQHCCKCPECYEVTRLAALRRLEPPGYGDWQVPDPYGPSGGNGASSGYGGYSSQTLPSQAGATPTPRTKAKLIPTGRDVGPVPPKPVPGKNTPKLNGSGPSWWPECTCTNRDWYEQASPAPLLVNPEALEPSLSVNGSDGMFKYEEIVLERGNSGLGFSIAGGIDNPHVPDDPGIFITKIIPGGAAAMDGRLGVNDCVLRVNEVDVSEVVHSRAVEALKEAGPVVRLVVRRRQPPPETIMEVNLLKGPKGLGFSIAGGIGNQHIPGDNSIYITKIIEGGAAQKDGRLQIGDRLLAVNNTNLQDVRHEEAVASLKNTSDMVYLKVAKPGSLHLNDMYAPPDYASTFTALADNHISHNSSLGYLGAVESKVTYPAPPQVPPTRYSPIPRHMLAEEDFTREPRKIILHKGSTGLGFNIVGGEDGEGIFVSFILAGGPADLSGELRRGDRILSVNGVNLRNATHEQAAAALKRAGQSVTIVAQYRPEEYSRFESKIHDLREQMMNSSMSSGSGSLRTSEKRSLYVRALFDYDRTRDSCLPSQGLSFSYGDILHVINASDDEWWQARLVTPHGESEQIGVIPSKKRVEKKERARLKTVKFHARTGMIESNRDFPGLSDDYYGAKNLKGVTSNTSDSESSSKGQEDAILSYEPVTRQEIHYARPVIILGPMKDRVNDDLISEFPHKFGSCVPHTTRPRRDNEVDGQDYHFVVSREQMEKDIQDNKFIEAGQFNDNLYGTSIQSVRAVAERGKHCILDVSGNAIKRLQQAQLYPIAIFIKPKSIEALMEMNRRQTYEQANKIFDKAMKLEQEFGEYFTAIVQGDSLEEIYNKIKQIIEDQSGHYIWVPSPEKL nmdz1_rat Glutamate receptor ionotropic, NMDA 1 N-methyl-D-aspartate receptor subunit NR1 Grin1 Nmdar1 Glutamate [NMDA] receptor subunit zeta-1 protein protein rat Rattus norvegicus (Rat) Rat MSTMHLLTFALLFSCSFARAACDPKIVNIGAVLSTRKHEQMFREAVNQANKRHGSWKIQLNATSVTHKPNAIQMALSVCEDLISSQVYAILVSHPPTPNDHFTPTPVSYTAGFYRIPVLGLTTRMSIYSDKSIHLSFLRTVPPYSHQSSVWFEMMRVYNWNHIILLVSDDHEGRAAQKRLETLLEERESKAEKVLQFDPGTKNVTALLMEARELEARVIILSASEDDAATVYRAAAMLNMTGSGYVWLVGEREISGNALRYAPDGIIGLQLINGKNESAHISDAVGVVAQAVHELLEKENITDPPRGCVGNTNIWKTGPLFKRVLMSSKYADGVTGRVEFNEDGDRKFANYSIMNLQNRKLVQVGIYNGTHVIPNDRKIIWPGGETEKPRGYQMSTRLKIVTIHQEPFVYVKPTMSDGTCKEEFTVNGDPVKKVICTGPNDTSPGSPRHTVPQCCYGFCIDLLIKLARTMNFTYEVHLVADGKFGTQERVNNSNKKEWNGMMGELLSGQADMIVAPLTINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQSTLWLLVGLSVHVVAVMLYLLDRFSPFGRFKVNSEEEEEDALTLSSAMWFSWGVLLNSGIGEGAPRSFSARILGMVWAGFAMIIVASYTANLAAFLVLDRPEERITGINDPRLRNPSDKFIYATVKQSSVDIYFRRQVELSTMYRHMEKHNYESAAEAIQAVRDNKLHAFIWDSAVLEFEASQKCDLVTTGELFFRSGFGIGMRKDSPWKQNVSLSILKSHENGFMEDLDKTWVRYQECDSRSNAPATLTFENMAGVFMLVAGGIVAGIFLIFIEIAYKRHKDARRKQMQLAFAAVNVWRKNLQDRKSGRAEPDPKKKATFRAITSTLASSFKRRRSSKDTSTGGGRGALQNQKDTVLPRRAIEREEGQLQLCSRHRES nmde2_rat Glutamate receptor ionotropic, NMDA 2B Grin2b Glutamate [NMDA] receptor subunit epsilon-2 N-methyl D-aspartate receptor subtype 2B protein protein rat Rattus norvegicus (Rat) Rat MKPSAECCSPKFWLVLAVLAVSGSKARSQKSPPSIGIAVILVGTSDEVAIKDAHEKDDFHHLSVVPRVELVAMNETDPKSIITRICDLMSDRKIQGVVFADDTDQEAIAQILDFISAQTLTPILGIHGGSSMIMADKDESSMFFQFGPSIEQQASVMLNIMEEYDWYIFSIVTTYFPGYQDFVNKIRSTIENSFVGWELEEVLLLDMSLDDGDSKIQNQLKKLQSPIILLYCTKEEATYIFEVANSVGLTGYGYTWIVPSLVAGDTDTVPSEFPTGLISVSYDEWDYGLPARVRDGIAIITTAASDMLSEHSFIPEPKSSCYNTHEKRIYQSNMLNRYLINVTFEGRNLSFSEDGYQMHPKLVIILLNKERKWERVGKWKDKSLQMKYYVWPRMCPETEEQEDDHLSIVTLEEAPFVIVESVDPLSGTCMRNTVPCQKRIISENKTDEEPGYIKKCCKGFCIDILKKISKSVKFTYDLYLVTNGKHGKKINGTWNGMIGEVVMKRAYMAVGSLTINEERSEVVDFSVPFIETGISVMVSRSNGTVSPSAFLEPFSADVWVMMFVMLLIVSAVAVFVFEYFSPVGYNRCLADGREPGGPSFTIGKAIWLLWGLVFNNSVPVQNPKGTTSKIMVSVWAFFAVIFLASYTANLAAFMIQEEYVDQVSGLSDKKFQRPNDFSPPFRFGTVPNGSTERNIRNNYAEMHAYMGKFNQRGVDDALLSLKTGKLDAFIYDAAVLNYMAGRDEGCKLVTIGSGKVFASTGYGIAIQKDSGWKRQVDLAILQLFGDGEMEELEALWLTGICHNEKNEVMSSQLDIDNMAGVFYMLGAAMALSLITFICEHLFYWQFRHCFMGVCSGKPGMVFSISRGIYSCIHGVAIEERQSVMNSPTATMNNTHSNILRLLRTAKNMANLSGVNGSPQSALDFIRRESSVYDISEHRRSFTHSDCKSYNNPPCEENLFSDYISEVERTFGNLQLKDSNVYQDHYHHHHRPHSIGSTSSIDGLYDCDNPPFTTQPRSISKKPLDIGLPSSKHSQLSDLYGKFSFKSDRYSGHDDLIRSDVSDISTHTVTYGNIEGNAAKRRKQQYKDSLKKRPASAKSRREFDEIELAYRRRPPRSPDHKRYFRDKEGLRDFYLDQFRTKENSPHWEHVDLTDIYKERSDDFKRDSVSGGGPCTNRSHLKHGTGEKHGVVGGVPAPWEKNLTNVDWEDRSGGNFCRSCPSKLHNYSSTVAGQNSGRQACIRCEACKKAGNLYDISEDNSLQELDQPAAPVAVTSNASSTKYPQSPTNSKAQKKNRNKLRRQHSYDTFVDLQKEEAALAPRSVSLKDKGRFMDGSPYAHMFEMPAGESSFANKSSVPTAGHHHNNPGSGYMLSKSLYPDRVTQNPFIPTFGDDQCLLHGSKSYFFRQPTVAGASKTRPDFRALVTNKPVVVTLHGAVPGRFQKDICIGNQSNPCVPNNKNPRAFNGSSNGHVYEKLSSIESDV q8cgm3_rat GRIN2A protein protein rat Rattus norvegicus (Rat) Rat MGRLGYWTLLVLPALLVWRDPAQNAAAEKGPPALNIAVLLGHSHDVTERELRNLWGPEQATGLPLDVNVVALL dlg1_mouse Disks large homolog 1 Synapse-associated protein 97 Embryo-dlg/synapse-associated protein 97 Dlg1 Dlgh1 protein protein mouse Mus musculus Mouse MPVRKQDTQRALHLLEEYRSKLSQTEDRQLRSSIERVINIFQSNLFQALIDIQEFYEVTLLDNPKCVDHSKQCEPVQPVTTWEIASLPSTAVTSETLPGSLSPPVEKYRYQDEEVLPPEHISPQVTNEVLGPELVHVSEKNLSEIENVHGFVSHSHISPIKPTEAVPPSSPIVPVTPALPVPAESTVVLPSAPQANPPPVLVNTDSLETPTYVNGTDADYEYEEITLERGNSGLGFSIAGGTDNPHIGDDSSIFITKIITGGAAAQDGRLRVNDCILRVNEADVRDVTHSKAVEALKEAGSIVRLYVKRRKPASEKIMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGKLQIGDKLLAVNSVCLEEVTHEEAVTALKNTSDFVYLKVAKPTSMYINDGYAPPDITNSSSQSVDNHVSPSSCLGQTPTSPARYSPISKAVLGDDEITREPRKVVLHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDRIISVNSVDLRAASHEQAAAALKNAGQAVTIVAQYRPEEYSRFEAKIHDLREQMMNSSVSSGSGSLRTSQKRSLYVRALFDYDKTKDSGLPSQGLNFRFGDILHVINASDDEWWQARQVTPDGESDEVGVIPSKRRVEKKERARLKTVKFNSKTRGDKGEIPDDMGSKGLKHVTSNASDSESSYRGQEEYVLSYEPVNQQEVNYTRPVIILGPMKDRVNDDLISEFPDKFGSCVPHTTRPKRDYEVDGRDYHFVTSREQMEKDIQEHKFIEAGQYNNHLYGTSVQSVRAVAEKGKHCILDVSGNAIKRLQIAQLYPISIFIKPKSMENIMEMNKRLTEEQARKTFERAMKLEQEFTEHFTAIVQGDTLEDIYNQVKQIIEEQSGPYIWVPAKEKL lrfn2_mouse Leucine-rich repeat and fibronectin type-III domain-containing protein 2 Lrfn2 Kiaa1246 Salm1 protein protein mouse Mus musculus Mouse METLLGGLLAFGMAFAVVDACPKYCVCQNLSESLGTLCPSKGLLFVPPDIDRRTVELRLGGNFIIHIGRQDFANMTGLVDLTLSRNTISHIQPFSFLDLESLRSLHLDSNRLPSLGEDTLRGLVNLQHLIVNNNQLGGIADDAFEDFLLTLEDLDLSYNNLHGLPWDSVRRMVNLHQLSLDHNLLDHIAEGTFADLQKLARLDLTSNRLQKLPPDPIFARSQASLLTATPFAPPLSFSFGGNPLHCNCELLWLRRLERDDDLETCGSPGSLKGRYFWHIREEEFVCEPPLITQHTHKLLVLEGQAATLKCKAIGDPSPLIHWVAPDDRLVGNSSRTAVYDNGTLDILITTSQDSGPFTCIAANAAGEATATVEVSIVQLPHLSNSTSRMAPPKSRLSDITGSSKTSRGGGGSGAGEPPKSTPERAVLVSDVTTTSALVKWSVSKSAPRVKMYQLQYNCSDDEVLIYRMIPASNKAFVVNNLVSGTGYDLCVLAMWDDTATTLTATNIVGCAQFFTKADYPQCQSMHSQILGGTMILVIGGIIVATLLVFIVILMVRYKVCNHDTPGKMAAATVSNVYSQTNGSQPPPLGGIPVGQLPQAPPKVVVRNELMDFSTSLARACDSSSSSSLGSGEAAGLGRGPWRLPPPAPRPKPSLDRLMGAFASLDLKSQRKEELLDSRTPAGRGAGTSSRGHHSDREPLLGPPATRARSLLPLPLEGKAKRSHSFDMGDFAAAAAAVPGGYSPPRRVSNIWTKRSLSVNGMLLPFEESDLVGARGTFGSSEWVMESTV dlg4_mouse Disks large homolog 4 Dlg4 Dlgh4 Psd95 Postsynaptic density protein 95 Synapse-associated protein 90 protein protein mouse Mus musculus Mouse MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKIIEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFHFGDVLHVIDASDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL dlg3_mouse Disks large homolog 3 Dlg3 Dlgh3 Synapse-associated protein 102 protein protein mouse Mus musculus Mouse MHKHQHCCKCPECYEVTRLAALRRLEPPGYGDWQVPDPYGPSGGNGASSGYGGYSSQTLPSQAGATPTPRTKAKLIPTGRDVGPVPPKPVPGKSTPKLNGSGPGWWPECTCTNRDWYEQASPAPLLVNPEALEPSLSVNGSDGMFKYEEIVLERGNSGLGFSIAGGIDNPHVPDDPGIFITKIIPGGAAAMDGRLGVNDCVLRVNEVDVSEVVHSRAVEALKEAGPVVRLVVRRRQPPPETIMEVNLLKGPKGLGFSIAGGIGNQHIPGDNSIYITKIIEGGAAQKDGRLQIGDRLLAVNNTNLQDVRHEEAVASLKNTSDMVYLKVAKPGSIHLNDMYAPPDYASTFTALADNHISHNSSLGYLGAVESKVTYPAPPQVPPTRYSPIPRHMLAEEDFTREPRKIILHKGSTGLGFNIVGGEDGEGIFVSFILAGGPADLSGELRRGDRILSVNGVNLRNATHEQAAAALKRAGQSVTIVAQYRPEEYSRFESKIHDLREQMMNSSMSSGSGSLRTSEKRSLYVRALFDYDRTRDSCLPSQGLSFSYGDILHVINASDDEWWQARLVTPHGESEQIGVIPSKKRVEKKERARLKTVKFHARTGMIESNRDFPGLSDDYYGAKNLKGVTSNTSDSESSSKGQEDAILSYEPVTRQEIHYARPVIILGPMKDRVNDDLISEFPHKFGSCVPHTTRPRRDNEVDGQDYHFVVSREQMEKDIQDNKFIEAGQFNDNLYGTSIQSVRAVAERGKHCILDVSGNAIKRLQQAQLYPIAIFIKPKSIEALMEMNRRQTYEQANKIFDKAMKLEQEFGEYFTAIVQGDSLEEIYNKIKQIIEDQSGHYIWVPSPEKL p35439-1 Glutamate receptor ionotropic, NMDA 1 Grin1 Nmdar1 N-methyl-D-aspartate receptor subunit NR1 Glutamate [NMDA] receptor subunit zeta-1 protein protein rat Rattus norvegicus (Rat) Rat MSTMHLLTFALLFSCSFARAACDPKIVNIGAVLSTRKHEQMFREAVNQANKRHGSWKIQLNATSVTHKPNAIQMALSVCEDLISSQVYAILVSHPPTPNDHFTPTPVSYTAGFYRIPVLGLTTRMSIYSDKSIHLSFLRTVPPYSHQSSVWFEMMRVYNWNHIILLVSDDHEGRAAQKRLETLLEERESKAEKVLQFDPGTKNVTALLMEARELEARVIILSASEDDAATVYRAAAMLNMTGSGYVWLVGEREISGNALRYAPDGIIGLQLINGKNESAHISDAVGVVAQAVHELLEKENITDPPRGCVGNTNIWKTGPLFKRVLMSSKYADGVTGRVEFNEDGDRKFANYSIMNLQNRKLVQVGIYNGTHVIPNDRKIIWPGGETEKPRGYQMSTRLKIVTIHQEPFVYVKPTMSDGTCKEEFTVNGDPVKKVICTGPNDTSPGSPRHTVPQCCYGFCIDLLIKLARTMNFTYEVHLVADGKFGTQERVNNSNKKEWNGMMGELLSGQADMIVAPLTINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQSTLWLLVGLSVHVVAVMLYLLDRFSPFGRFKVNSEEEEEDALTLSSAMWFSWGVLLNSGIGEGAPRSFSARILGMVWAGFAMIIVASYTANLAAFLVLDRPEERITGINDPRLRNPSDKFIYATVKQSSVDIYFRRQVELSTMYRHMEKHNYESAAEAIQAVRDNKLHAFIWDSAVLEFEASQKCDLVTTGELFFRSGFGIGMRKDSPWKQNVSLSILKSHENGFMEDLDKTWVRYQECDSRSNAPATLTFENMAGVFMLVAGGIVAGIFLIFIEIAYKRHKDARRKQMQLAFAAVNVWRKNLQDRKSGRAEPDPKKKATFRAITSTLASSFKRRRSSKDTSTGGGRGALQNQKDTVLPRRAIEREEGQLQLCSRHRES p35439-4 Glutamate receptor ionotropic, NMDA 1 Grin1 Nmdar1 N-methyl-D-aspartate receptor subunit NR1 Glutamate [NMDA] receptor subunit zeta-1 protein protein rat Rattus norvegicus (Rat) Rat MSTMHLLTFALLFSCSFARAACDPKIVNIGAVLSTRKHEQMFREAVNQANKRHGSWKIQLNATSVTHKPNAIQMALSVCEDLISSQVYAILVSHPPTPNDHFTPTPVSYTAGFYRIPVLGLTTRMSIYSDKSIHLSFLRTVPPYSHQSSVWFEMMRVYNWNHIILLVSDDHEGRAAQKRLETLLEERESKAEKVLQFDPGTKNVTALLMEARELEARVIILSASEDDAATVYRAAAMLNMTGSGYVWLVGEREISGNALRYAPDGIIGLQLINGKNESAHISDAVGVVAQAVHELLEKENITDPPRGCVGNTNIWKTGPLFKRVLMSSKYADGVTGRVEFNEDGDRKFANYSIMNLQNRKLVQVGIYNGTHVIPNDRKIIWPGGETEKPRGYQMSTRLKIVTIHQEPFVYVKPTMSDGTCKEEFTVNGDPVKKVICTGPNDTSPGSPRHTVPQCCYGFCIDLLIKLARTMNFTYEVHLVADGKFGTQERVNNSNKKEWNGMMGELLSGQADMIVAPLTINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQSTLWLLVGLSVHVVAVMLYLLDRFSPFGRFKVNSEEEEEDALTLSSAMWFSWGVLLNSGIGEGAPRSFSARILGMVWAGFAMIIVASYTANLAAFLVLDRPEERITGINDPRLRNPSDKFIYATVKQSSVDIYFRRQVELSTMYRHMEKHNYESAAEAIQAVRDNKLHAFIWDSAVLEFEASQKCDLVTTGELFFRSGFGIGMRKDSPWKQNVSLSILKSHENGFMEDLDKTWVRYQECDSRSNAPATLTFENMAGVFMLVAGGIVAGIFLIFIEIAYKRHKDARRKQMQLAFAAVNVWRKNLQDRKSGRAEPDPKKKATFRAITSTLASSFKRRRSSKDTQYHPTDITGPLNLSDPSVSTVV lrfn1_mouse Leucine-rich repeat and fibronectin type III domain-containing protein 1 Salm2 Semo1 Synaptic adhesion-like molecule 2 Synaptic differentiation-enhancing molecule 1 Lrfn1 protein protein mouse Mus musculus Mouse MAPGPFSSGLFSPPPAALPFLLLLWAGASRGQPCPGRCICQNVAPTLTMLCAKTGLLFVPPAIDRRVVELRLTDNFIAAVRRRDFANMTSLVHLTLSRNTIGQVAAGAFADLRALRALHLDSNRLAEVRGDQLRGLGNLRHLILGNNQIRKVESAAFDAFLSTVEDLDLSYNNLEALPWEAVGQMVNLNTLTLDHNLIDHIAEGTFVQLHKLVRLDMTSNRLHKLPPDGLFLRSQGGGPKPPTPLTVSFGGNPLHCNCELLWLRRLTREDDLETCATPEHLTDRYFWSIPEEEFLCEPPLITRQAGGRALVVEGQAVSLRCRAVGDPEPVVHWVAPDGRLLGNSSRTRVRGDGTLDVTITTLRDSGTFTCIASNAAGEATAPVEVCVVPLPLMAPPPAAPPPLTEPGSSDIATPGRPGANDSTSERRLVAAELTSSSVLIRWPAQRPVPGIRMYQVQYNSSADDSLVYRMIPSTSQTFLVNDLAAGRAYDLCVLAVYDDGATALPATRVVGCVQFTTAGDPAPCRPLRAHFLGGTMIIAIGGVIVASVLVFIVLLMIRYKVYGDGDSRRIKGTSRTPPRVSHVCSQTNGAGAQQASAPPAPDRYEALREVAVPAAIEAKAMEAEATSTELEVVLGRSLGGSATSLCLLPSEETSGEESRAMTGPRRSRSGALGPPTSAPPTLALVPGGAPARPRPQQRYSFDGDYGALFQSHSYPRRARRTKRHRSTPHLDGAGGGAAGEDGDLGLGSARARLAFTSTEWMLESTV salm1-dlg4-1 Co-immunoprecipitation analysis reveals interaction between the SALM1 protein and Dlg4 protein 338444 N/A 338450 unspecified role unspecified role prey prey N/A 338451 unspecified role unspecified role bait bait physical association physical association false false false salm1-dlg1-1 Co-immunoprecipitation analysis reveals interaction between the SALM1 protein and Dlg1 protein 338444 N/A 338451 unspecified role unspecified role bait bait N/A 338452 unspecified role unspecified role prey prey physical association physical association false false false salm1-dlg3 Co-immunoprecipitation analysis reveals interaction between the SALM1 protein and Dlg3 protein 338444 N/A 338453 unspecified role unspecified role prey prey N/A 338451 unspecified role unspecified role bait bait physical association physical association false false false dlg3-salm1 Co-immunoprecipitation analysis reveals interaction between the Dlg3 protein and SALM1 protein 338444 N/A 338451 unspecified role unspecified role prey prey N/A 338453 unspecified role unspecified role bait bait physical association physical association false false false dlg4-salm1-1 Co-immunoprecipitation analysis reveals interaction between the Dlg4 protein and SALM1 protein 338444 N/A 338450 unspecified role unspecified role bait bait N/A 338451 unspecified role unspecified role prey prey physical association physical association false false false dlg1-salm1-2 Co-immunoprecipitation analysis reveals interaction between the Dlg1 protein and SALM1 protein 338444 N/A 338451 unspecified role unspecified role prey prey N/A 338452 unspecified role unspecified role bait bait physical association physical association false false false salm1-nr1 Co-immunoprecipitation analysis reveals interaction between the SALM1 protein and NR1 protein 338444 N/A 338454 unspecified role unspecified role prey prey N/A 338451 unspecified role unspecified role bait bait physical association physical association false false false salm1-nr2b Co-immunoprecipitation analysis reveals interaction between the SALM1 protein and NR2B protein 338444 N/A 338455 unspecified role unspecified role prey prey N/A 338451 unspecified role unspecified role bait bait physical association physical association false false false salm1-nr2a-2 Co-immunoprecipitation analysis reveals interaction between the SALM1 protein and NR2A protein 338444 N/A 338451 unspecified role unspecified role bait bait N/A 338456 unspecified role unspecified role prey prey physical association physical association false false false nr2a-salm1 Co-immunoprecipitation analysis reveals interaction between the NR2A protein and SALM1 protein 338444 N/A 338451 unspecified role unspecified role prey prey N/A 338456 unspecified role unspecified role bait bait physical association physical association false false false nr2b-salm1 Co-immunoprecipitation analysis reveals interaction between the NR2B protein and SALM1 protein 338444 N/A 338455 unspecified role unspecified role bait bait N/A 338451 unspecified role unspecified role prey prey physical association physical association false false false nr1-salm1 Co-immunoprecipitation analysis reveals interaction between the NR1 protein and SALM1 protein 338444 N/A 338451 unspecified role unspecified role prey prey N/A 338454 unspecified role unspecified role bait bait physical association physical association false false false dlg1-salm1-1 Co-immunoprecipitation analysis reveals interaction between the Dlg1 protein and SALM1 protein 338445 N/A 338457 unspecified role unspecified role bait bait N/A 338458 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false dlg4-salm1 Co-immunoprecipitation analysis reveals interaction between the Dlg4 protein and SALM1 protein 338445 N/A 338458 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 338459 unspecified role unspecified role bait bait physical association physical association false false false dlg3-salm1-1 Co-immunoprecipitation analysis reveals interaction between the Dlg3 protein and SALM1 protein 338445 N/A 338460 unspecified role unspecified role bait bait N/A 338458 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false nr1_1-salm1 Co-immunoprecipitation analysis reveals interaction between the NR1-1 protein and SALM1 protein 338445 N/A 338458 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 338461 unspecified role unspecified role bait bait physical association physical association false false false salm1-nr1_1 Co-immunoprecipitation analysis reveals interaction between the SALM1 protein and NR1-1 protein 338445 N/A 338458 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 338461 unspecified role unspecified role prey prey physical association physical association false false false nr1_4-salm1 Co-immunoprecipitation analysis reveals interaction between the NR1-4 protein and SALM1 protein 338445 N/A 338458 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 338462 unspecified role unspecified role bait bait physical association physical association false false false salm1-nr1_4 Co-immunoprecipitation analysis reveals interaction between the SALM1 protein and NR1-4 protein 338445 N/A 338458 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 338462 unspecified role unspecified role prey prey physical association physical association false false false salm1-nr1-1 Co-immunoprecipitation analysis reveals interaction between the SALM1 protein and NR1 protein 338445 N/A 338458 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 338454 unspecified role unspecified role prey prey region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false salm1-dlg4 Co-immunoprecipitation analysis reveals interaction between the SALM1 protein and Dlg4 protein 338446 N/A 338459 unspecified role unspecified role prey prey N/A 338458 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false salm1-dlg1 Co-immunoprecipitation analysis reveals interaction between the SALM1 protein and Dlg1 protein 338446 N/A 338457 unspecified role unspecified role prey prey N/A 338458 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false nr1-salm1-1 Co-immunoprecipitation analysis reveals interaction between the NR1 protein and SALM1 protein 338446 N/A 338458 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 338454 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis range range range range false region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false salm1-dlg4-3 Colocalisation analysis reveals interaction between the SALM1 protein and Dlg4 protein 338447 N/A 338458 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 338450 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Removal of amino acid residues 784 to 788 of SALM1 reduced colocalisation of the proteins. The authors state that 35% of SALM1 puncta observed in rat neurons transfected with mosue SALM1-myc colocalised with the synaptic marker synapsin (data not shown in paper). salm1-nr2a Colocalisation analysis reveals interaction between the SALM1 protein and NR2A protein 338447 N/A 338456 unspecified role unspecified role neutral component neutral component N/A 338458 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Removal of amino acid residues 784 to 788 of SALM1 reduced colocalisation of the proteins. It is assumed, although not stated in the text, that NR2A is a protein of rat origin. salm1-nr2a-1 Colocalisation analysis reveals interaction between the SALM1 protein and NR2A protein 338447 N/A 338456 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 338458 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. Area of co-loaclisation of full length SALM1 and NR2A-GFP was 48.4 +/- 9.6% and NR2A-GFP total 56.5 +/- 10.4%. Colocalisation of SALM1-myc (1-784)and NR2A-GFP was 18.0 +/- 6.0% and total NR2A-GFP 29.9 +/- 10.4%. Removal of amino acid residues 784 to 788 of SALM1 reduced colocalisation of the proteins. Both the number of NR2A surface puncta and the intensity of NR2A staining was significantly higher for neurons co-transfected with SALM1-myc than SALM1-myc trunacted by the last 4 amino acids (784-788) or vector alone. Area of co-loaclisation of full length SALM1 and NR2A-GFP was 48.4 +/- 9.6% and NR2A-GFP total 56.5 +/- 10.4%. Colocalisation of SALM1-myc (1-784)and NR2A-GFP was 18.0 +/- 6.0% and total NR2A-GFP 29.9 +/- 10.4%. dlg1-salm1 Yeast 2 hybrid analysis reveals interaction between the Dgl1 protein and SALM1 protein 338448 N/A 338458 unspecified role unspecified role prey prey N/A 338457 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false dlg1-salm2 Yeast 2 hybrid analysis reveals interaction between the Dgl1 protein and SALM2 protein 338448 N/A 338463 unspecified role unspecified role prey prey N/A 338457 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false salm1-dlg4-2 Colocalisation analysis reveals interaction between the SALM1 protein and Dlg4 protein 338449 N/A 338450 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 338451 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false