IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ cubelos-2005-1 The scaffolding protein PSD-95 interacts with the glycine transporter GLYT1 and impairs its internalization. rat-brain Rattus norvegicus brain brain Brain [cerebrum] far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Cubelos B., Gonzalez-Gonzalez IM., Gimenez C., Zafra F. Synapse - Interactions of proteins with an established role in the presynapse. Rat brain synaptosomal extracts were incubated an antibody array membrane wich contains 400 different antibodies. The proteins retained were then incubated with biotinylated anti-GLYT1 antibody and then with spreptavidin peroxidase. The interaction was detected by ECL. fig 1A fzafra@cbm.uam.es J. Neurochem. (0022-3042) 2005 Only protein-protein interactions imex curation cubelos-2005-2 The scaffolding protein PSD-95 interacts with the glycine transporter GLYT1 and impairs its internalization. rat-brain Rattus norvegicus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cubelos B., Gonzalez-Gonzalez IM., Gimenez C., Zafra F. Synapse - Interactions of proteins with an established role in the presynapse. Rat brain synaptosomal extracts were incubated an antibody array membrane wich contains 400 different antibodies. The proteins retained were then incubated with biotinylated anti-GLYT1 antibody and then with spreptavidin peroxidase. The interaction was detected by ECL. fig 1A fzafra@cbm.uam.es J. Neurochem. (0022-3042) 2005 Only protein-protein interactions imex curation cubelos-2005-3 The scaffolding protein PSD-95 interacts with the glycine transporter GLYT1 and impairs its internalization. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cubelos B., Gonzalez-Gonzalez IM., Gimenez C., Zafra F. Synapse - Interactions of proteins with an established role in the presynapse. Rat brain synaptosomal extracts were incubated an antibody array membrane wich contains 400 different antibodies. The proteins retained were then incubated with biotinylated anti-GLYT1 antibody and then with spreptavidin peroxidase. The interaction was detected by ECL. fig 1A fzafra@cbm.uam.es J. Neurochem. (0022-3042) 2005 Only protein-protein interactions imex curation cubelos-2005-4 The scaffolding protein PSD-95 interacts with the glycine transporter GLYT1 and impairs its internalization. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Cubelos B., Gonzalez-Gonzalez IM., Gimenez C., Zafra F. Synapse - Interactions of proteins with an established role in the presynapse. Rat brain synaptosomal extracts were incubated an antibody array membrane wich contains 400 different antibodies. The proteins retained were then incubated with biotinylated anti-GLYT1 antibody and then with spreptavidin peroxidase. The interaction was detected by ECL. fig 1A fzafra@cbm.uam.es J. Neurochem. (0022-3042) 2005 Only protein-protein interactions imex curation cubelos-2005-5 The scaffolding protein PSD-95 interacts with the glycine transporter GLYT1 and impairs its internalization. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Cubelos B., Gonzalez-Gonzalez IM., Gimenez C., Zafra F. Synapse - Interactions of proteins with an established role in the presynapse. Rat brain synaptosomal extracts were incubated an antibody array membrane wich contains 400 different antibodies. The proteins retained were then incubated with biotinylated anti-GLYT1 antibody and then with spreptavidin peroxidase. The interaction was detected by ECL. fig 1A fzafra@cbm.uam.es J. Neurochem. (0022-3042) 2005 Only protein-protein interactions imex curation cubelos-2005-6 The scaffolding protein PSD-95 interacts with the glycine transporter GLYT1 and impairs its internalization. rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Cubelos B., Gonzalez-Gonzalez IM., Gimenez C., Zafra F. Synapse - Interactions of proteins with an established role in the presynapse. Rat brain synaptosomal extracts were incubated an antibody array membrane wich contains 400 different antibodies. The proteins retained were then incubated with biotinylated anti-GLYT1 antibody and then with spreptavidin peroxidase. The interaction was detected by ECL. fig 1A fzafra@cbm.uam.es J. Neurochem. (0022-3042) 2005 Only protein-protein interactions imex curation dlg4_rat Disks large homolog 4 Dlg4 Dlgh4 Postsynaptic density protein 95 Synapse-associated protein 90 Psd95 protein protein rat Rattus norvegicus (Rat) Rat MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKVMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVIDAGDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL sc6a9_rat Sodium- and chloride-dependent glycine transporter 1 Solute carrier family 6 member 9 Slc6a9 protein protein rat Rattus norvegicus (Rat) Rat MAVAHGPVATSSPEQNGAVPSEATKKDQNLTRGNWGNQIEFVLTSVGYAVGLGNVWRFPYLCYRNGGGAFMFPYFIMLVFCGIPLFFMELSFGQFASQGCLGVWRISPMFKGVGYGMMVVSTYIGIYYNVVICIAFYYFFSSMTHVLPWAYCNNPWNTPDCAGVLDASNLTNGSRPTALSGNLSHLFNYTLQRTSPSEEYWRLYVLKLSDDIGDFGEVRLPLLGCLGVSWVVVFLCLIRGVKSSGKVVYFTATFPYVVLTILFVRGVTLEGAFTGIMYYLTPKWDKILEAKVWGDAASQIFYSLGCAWGGLITMASYNKFHNNCYRDSVIISITNCATSVYAGFVIFSILGFMANHLGVDVSRVADHGPGLAFVAYPEALTLLPISPLWSLLFFFMLILLGLGTQFCLLETLVTAIVDEVGNEWILQKKTYVTLGVAVAGFLLGIPLTSQAGIYWLLLMDNYAASFSLVVISCIMCVSIMYIYGHRNYFQDIQMMLGFPPPLFFQICWRFVSPTIIFFILIFTVIQYRPITYNHYQYPGWAVAIGFLMALSSVICIPLYALFQLCRTDGDTLLQRLKNATKPSRDWGPALLEHRTGRYAPTTTPSPEDGFEVQPLHPDKAQIPIVGSNGSSRLQDSRI ita1_rat Integrin alpha-1 Laminin and collagen receptor VLA-1 CD49 antigen-like family member A Itga1 protein protein rat Rattus norvegicus (Rat) Rat MVPRRPASLEVTVACIWLLTVILGFCVSFNVDVKNSMSFSGPVEDMFGYTVQQYENEEGKWVLIGSPLVGQPKARTGDVYKCPVGRERAMPCVKLDLPVNTSIPNVTEIKENMTFGSTLVTNPNGGFLACGPLYAYRCGHLHYTTGICSDVSPTFQVVNSFAPVQECSTQLDIVIVLDGSNSIYPWESVIAFLNDLLKRMDIGPKQTQVGIVQYGENVTHEFNLNKYSSTEEVLVAANKIGRQGGLQTMTALGIDTARKEAFTEARGARRGVKKVMVIVTDGESHDNYRLKQVIQDCEDENIQRFSIAILGHYNRGNLSTEKFVEEIKSIASEPTEKHFFNVSDELALVTIVKALGERIFALEATADQSAASFEMEMSQTGFSAHYSQDWVMLGAVGAYDWNGTVVMQKANQMVIPHNTTFQTEPAKMNEPLASYLGYTVNSATIPGDVLYIAGQPRYNHTGQVVIYKMEDGNINILQTLGGEQIGSYFGSVLTTIDIDKDSYTDLLLVGAPMYMGTEKEEQGKVYVYAVNQTRFEYQMSLEPIRQTCCSSLKDNSCTKENKNEPCGARFGTAIAAVKDLNVDGFNDVVIGAPLEDDHAGAVYIYHGSGKTIREAYAQRIPSGGDGKTLKFFGQSIHGEMDLNGDGLTDVTIGGLGGAALFWARDVAVVKVTMNFEPNKVNIQKKNCRVEGKETVCINATMCFHVKLKSKEDSIYEADLQYRVTLDSLRQISRSFFSGTQERKIQRNITVRESECIRHSFYMLDKHDFQDSVRVTLDFNLTDPENGPVLDDALPNSVHEHIPFAKDCGNKERCISDLTLNVSTTEKSLLIVKSQHDKFNVSLTVKNKGDSAYNTRTVVQHSPNLIFSGIEEIQKDSCESNQNITCRVGYPFLRAGETVTFKIIFQFNTSHLSENAIIHLSATSDSEEPLESLNDNEVNISIPVKYEVGLQFYSSASEHHISVAANETIPEFINSTEDIGNEINVFYTIRKRGHFPMPELQLSISFPNLTADGYPVLYPIGWSSSDNVNCRPRSLEDPFGINSGKKMTISKSEVLKRGTIQDCSSTCGVATITCSLLPSDLSQVNVSLLLWKPTFIRAHFSSLNLTLRGELKSENSSLTLSSSNRKRELAIQISKDGLPGRVPLWVILLSAFAGLLLLMLLILALWKIGFFKRPLKKKMEK p28572-2 Sodium- and chloride-dependent glycine transporter 1 GlyT-1B GlyT-1 Slc6a9 Solute carrier family 6 member 9 protein protein rat Rattus norvegicus (Rat) Rat MAVAHGPVATSSPEQNGAVPSEATKKDQNLTRGNWGNQIEFVLTSVGYAVGLGNVWRFPYLCYRNGGGAFMFPYFIMLVFCGIPLFFMELSFGQFASQGCLGVWRISPMFKGVGYGMMVVSTYIGIYYNVVICIAFYYFFSSMTHVLPWAYCNNPWNTPDCAGVLDASNLTNGSRPTALSGNLSHLFNYTLQRTSPSEEYWRLYVLKLSDDIGDFGEVRLPLLGCLGVSWVVVFLCLIRGVKSSGKVVYFTATFPYVVLTILFVRGVTLEGAFTGIMYYLTPKWDKILEAKVWGDAASQIFYSLGCAWGGLITMASYNKFHNNCYRDSVIISITNCATSVYAGFVIFSILGFMANHLGVDVSRVADHGPGLAFVAYPEALTLLPISPLWSLLFFFMLILLGLGTQFCLLETLVTAIVDEVGNEWILQKKTYVTLGVAVAGFLLGIPLTSQAGIYWLLLMDNYAASFSLVVISCIMCVSIMYIYGHRNYFQDIQMMLGFPPPLFFQICWRFVSPTIIFFILIFTVIQYRPITYNHYQYPGWAVAIGFLMALSSVICIPLYALFQLCRTDGDTLLQRLKNATKPSRDWGPALLEHRTGRYAPTTTPSPEDGFEVQPLHPDKAQIPIVGSNGSSRLQDSRI glyt1-dlg4-1 Interaction between Glyt1 and PSD95 detected by antibody array 328347 N/A 328353 unspecified role unspecified role prey prey N/A 328354 unspecified role unspecified role bait bait physical association physical association false false false glyt1-itga1-1 Interaction between Glyt1 and integrin alpha 1 detected by antibody array 328347 N/A 328355 unspecified role unspecified role prey prey N/A 328354 unspecified role unspecified role bait bait physical association physical association false false false glyt1-dlg4-2 Interaction between Glyt1 and PSD95 detected by co-immunoprecipitation 328348 N/A 328354 unspecified role unspecified role bait bait N/A 328353 unspecified role unspecified role prey prey physical association physical association false false false glyt1-dlg4-3 Interaction between Glyt1 and PSD95 detected by co-immunoprecipitation 328349 N/A 328356 unspecified role unspecified role bait bait gly-arg-ile sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 328353 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false P28572-2; P31016 overexpressed Deletion of the last 3 amino-acids or addition of an Alanine at the c-terminus of Glyt1 disrupt the interaction with PSD95. glyt1-dlg4-4 Interaction between Glyt1 and PSD95 detected by pull-down 328350 N/A 328356 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false gly-arg-ile sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 328353 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association false false false glyt1-dlg4-5 Interaction between Glyt1 and PSD95 detected yeast two-hybrid 328351 N/A 328356 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false undefined mutation mutation disrupting mutation disrupting interaction mutation disrupting deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 328353 unspecified role unspecified role prey prey pdz1 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false pdz2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false pdz3 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false While the strongest interaction was observed with the second PDZ domain, glyt1 also interacts with the first PDZ domain and weakly with the third one. glyt1-dlg4-6 Glyt1 recruits PSD-95 to the plasma membrane 328352 N/A 328353 unspecified role unspecified role neutral component neutral component cfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 328356 unspecified role unspecified role neutral component neutral component undefined mutation mutation disrupting mutation disrupting interaction mutation disrupting deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false P28572-2; P31016 over-expressed