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    <entry>
        <source releaseDate="2005-06-01+01:00">
            <names>
                <shortLabel>IntAct</shortLabel>
                <fullName>European Bioinformatics Institute</fullName>
                <alias typeAc="MI:1041" type="synonym">IntAct</alias>
                <alias typeAc="MI:1041" type="synonym">intact</alias>
            </names>
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            <attributeList>
                <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/</attribute>
                <attribute name="search-url" nameAc="MI:0615">http://www.ebi.ac.uk/intact/query/${ac}</attribute>
                <attribute name="id-validation-regexp" nameAc="MI:0628">EBI-[0-9]+|IA:[0-9]+</attribute>
                <attribute name="definition">INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions.</attribute>
                <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact</attribute>
                <attribute name="postaladdress">European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom</attribute>
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                <attribute name="url">http://www.ebi.ac.uk/</attribute>
            </attributeList>
        </source>
        <experimentList>
            <experimentDescription id="181378">
                <names>
                    <shortLabel>beites-2005-1</shortLabel>
                    <fullName>The septin Sept5/CDCrel-1 competes with alpha-SNAP for binding to the SNARE complex.</fullName>
                </names>
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                <hostOrganismList>
                    <hostOrganism ncbiTaxId="-1">
                        <names>
                            <shortLabel>in vitro</shortLabel>
                            <fullName>In vitro</fullName>
                        </names>
                    </hostOrganism>
                </hostOrganismList>
                <interactionDetectionMethod>
                    <names>
                        <shortLabel>coip</shortLabel>
                        <fullName>coimmunoprecipitation</fullName>
                        <alias typeAc="MI:0303" type="go synonym">co-immunoprecipitation</alias>
                        <alias typeAc="MI:0303" type="go synonym">CoIp</alias>
                        <alias typeAc="MI:0303" type="go synonym">immunoprecipitation</alias>
                        <alias typeAc="MI:0303" type="go synonym">Co-IP</alias>
                        <alias typeAc="MI:1041" type="synonym">co-immunoprecipitation</alias>
                        <alias typeAc="MI:1041" type="synonym">CoIp</alias>
                        <alias typeAc="MI:1041" type="synonym">immunoprecipitation</alias>
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                        <alias typeAc="MI:1041" type="synonym">coip</alias>
                    </names>
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                    </xref>
                </interactionDetectionMethod>
                <participantIdentificationMethod>
                    <names>
                        <shortLabel>polyclonal western</shortLabel>
                        <fullName>polyclonal antibody western blot</fullName>
                        <alias typeAc="MI:1041" type="synonym">polyclonal western</alias>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0086" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-964" refType="identity" refTypeAc="MI:0356"/>
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                </participantIdentificationMethod>
                <attributeList>
                    <attribute name="author-list" nameAc="MI:0636">Beites CL., Campbell KA., Trimble WS.</attribute>
                    <attribute name="dataset" nameAc="MI:0875">Synapse - Interactions of proteins with an established role in the presynapse.</attribute>
                    <attribute name="journal" nameAc="MI:0885">Biochem. J. (0264-6021)</attribute>
                    <attribute name="publication year" nameAc="MI:0886">2005</attribute>
                </attributeList>
            </experimentDescription>
        </experimentList>
        <interactorList>
            <interactor id="181379">
                <names>
                    <shortLabel>sept5_rat</shortLabel>
                    <fullName>Septin-5</fullName>
                    <alias typeAc="MI:0301" type="gene name">Septin5</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">Cell division control-related protein 1</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">Peanut-like protein 1</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">Pnutl1</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">Sept5</alias>
                </names>
                <xref>
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                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0044305" secondary="calyx of Held" version="SP_120"/>
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                <interactorType>
                    <names>
                        <shortLabel>protein</shortLabel>
                        <fullName>protein</fullName>
                    </names>
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                    </xref>
                </interactorType>
                <organism ncbiTaxId="10116">
                    <names>
                        <shortLabel>rat</shortLabel>
                        <fullName>Rattus norvegicus (Rat)</fullName>
                        <alias typeAc="MI:1041" type="synonym">Rat</alias>
                    </names>
                </organism>
                <sequence>MSTGLRYKSKLATPEDKQDIDKQYVGFATLPNQVHRKSVKKGFDFTLMVAGESGLGKSTLVHSLFLTDLYKDRKLLSAEERINQTVEILKHTVDIEEKGVKLKLTIVDTPGFGDAVNNFECWKPITDYVDQQFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPVDVGFMKALHEKVNIVPLIAKADCLVPSEIRKLKDRIREEIDKFGIHVYQFPECDSDEDEDFKQQDRELKESAPFAVIGSNTVVEAKGQRVRGRLYPWGIVEVENQAHCDFVKLRNMLIRTHMHDLKDVTCDVHYENYRAHCIQQMTSKLTQDSRMESPIPILPLPTPDSETEKLIRMKDEELRRMQEMLQKMKQRMQDQ</sequence>
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            <interactor id="181380">
                <names>
                    <shortLabel>stx1a_rat</shortLabel>
                    <fullName>Syntaxin-1A</fullName>
                    <alias typeAc="MI:0302" type="gene name synonym">Synaptotagmin-associated 35 kDa protein</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">Neuron-specific antigen HPC-1</alias>
                    <alias typeAc="MI:0301" type="gene name">Stx1a</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">Sap</alias>
                </names>
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                    <secondaryRef db="reactome" dbAc="MI:0467" id="R-RNO-5682910" version="SP_209"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0019900" secondary="kinase binding" version="SP_209"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0098815" secondary="modulation of excitatory postsynaptic potential" version="SP_209"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0016021" secondary="integral component of membrane" version="SP_209"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="5W5D" version="SP_209"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0006886" secondary="intracellular protein transport" version="SP_209"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0006906" secondary="vesicle fusion" version="SP_209"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0032991" secondary="protein-containing complex" version="SP_209"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0045956" secondary="positive regulation of calcium ion-dependent exocytosis" version="SP_209"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0048488" secondary="synaptic vesicle endocytosis" version="SP_209"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3J96" version="SP_209"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0016925" secondary="protein sumoylation" version="SP_209"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3RK2" version="SP_209"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0033605" secondary="positive regulation of catecholamine secretion" version="SP_209"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0042734" secondary="presynaptic membrane" version="SP_209"/>
                    <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSRNOT00000049601" version="SP_209" refType="transcript"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0042641" secondary="actomyosin" version="SP_209"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0032028" secondary="myosin head/neck binding" version="SP_209"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0070032" secondary="synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex" version="SP_209"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0070044" secondary="synaptobrevin 2-SNAP-25-syntaxin-1a complex" version="SP_209"/>
                </xref>
                <interactorType>
                    <names>
                        <shortLabel>protein</shortLabel>
                        <fullName>protein</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactorType>
                <organism ncbiTaxId="10116">
                    <names>
                        <shortLabel>rat</shortLabel>
                        <fullName>Rattus norvegicus (Rat)</fullName>
                        <alias typeAc="MI:1041" type="synonym">Rat</alias>
                    </names>
                </organism>
                <sequence>MKDRTQELRTAKDSDDDDDVTVTVDRDRFMDEFFEQVEEIRGFIDKIAENVEEVKRKHSAILASPNPDEKTKEELEELMSDIKKTANKVRSKLKSIEQSIEQEEGLNRSSADLRIRKTQHSTLSRKFVEVMSEYNATQSDYRERCKGRIQRQLEITGRTTTSEELEDMLESGNPAIFASGIIMDSSISKQALSEIETRHSEIIKLENSIRELHDMFMDMAMLVESQGEMIDRIEYNVEHAVDYVERAVSDTKKAVKYQSKARRKKIMIIICCVILGIIIASTIGGIFG</sequence>
            </interactor>
        </interactorList>
        <interactionList>
            <interaction id="181381">
                <names>
                    <shortLabel>gp1bb-stx1a-1</shortLabel>
                    <fullName>Interaction of Sept5 with Syntaxin detected by co-immunoprecipitation</fullName>
                </names>
                <xref>
                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-539733" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <experimentList>
                    <experimentRef>181378</experimentRef>
                </experimentList>
                <participantList>
                    <participant id="181382">
                        <names>
                            <shortLabel>N/A</shortLabel>
                        </names>
                        <xref>
                            <primaryRef db="intact" dbAc="MI:0469" id="EBI-539734" refType="identity" refTypeAc="MI:0356"/>
                        </xref>
                        <interactorRef>181379</interactorRef>
                        <biologicalRole>
                            <names>
                                <shortLabel>unspecified role</shortLabel>
                                <fullName>unspecified role</fullName>
                            </names>
                            <xref>
                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                            </xref>
                        </biologicalRole>
                        <experimentalRoleList>
                            <experimentalRole>
                                <names>
                                    <shortLabel>bait</shortLabel>
                                    <fullName>bait</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                </xref>
                            </experimentalRole>
                        </experimentalRoleList>
                        <featureList>
                            <feature id="181383">
                                <names>
                                    <shortLabel>region</shortLabel>
                                </names>
                                <xref>
                                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-539736" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                                <featureType>
                                    <names>
                                        <shortLabel>gst tag</shortLabel>
                                        <fullName>glutathione s tranferase tag</fullName>
                                        <alias typeAc="MI:0303" type="go synonym">glutathione S-tranferase tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">glutathione S-tranferase tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">gst tag</alias>
                                    </names>
                                    <xref>
                                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0519" refType="identity" refTypeAc="MI:0356"/>
                                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456510" refType="identity" refTypeAc="MI:0356"/>
                                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                    </xref>
                                </featureType>
                                <featureRangeList>
                                    <featureRange>
                                        <startStatus>
                                            <names>
                                                <shortLabel>undetermined</shortLabel>
                                                <fullName>undetermined sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">undetermined</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                                            </xref>
                                        </startStatus>
                                        <endStatus>
                                            <names>
                                                <shortLabel>undetermined</shortLabel>
                                                <fullName>undetermined sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">undetermined</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                                            </xref>
                                        </endStatus>
                                        <isLink>false</isLink>
                                    </featureRange>
                                </featureRangeList>
                            </feature>
                            <feature id="181384">
                                <names>
                                    <shortLabel>region</shortLabel>
                                </names>
                                <xref>
                                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-539738" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                                <featureType>
                                    <names>
                                        <shortLabel>binding region</shortLabel>
                                        <fullName>binding-associated region</fullName>
                                        <alias typeAc="MI:1041" type="synonym">binding region</alias>
                                    </names>
                                    <xref>
                                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0117" refType="identity" refTypeAc="MI:0356"/>
                                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456493" refType="identity" refTypeAc="MI:0356"/>
                                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                    </xref>
                                </featureType>
                                <featureRangeList>
                                    <featureRange>
                                        <startStatus>
                                            <names>
                                                <shortLabel>certain</shortLabel>
                                                <fullName>certain sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">certain</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                            </xref>
                                        </startStatus>
                                        <begin position="1"/>
                                        <endStatus>
                                            <names>
                                                <shortLabel>certain</shortLabel>
                                                <fullName>certain sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">certain</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                            </xref>
                                        </endStatus>
                                        <end position="213"/>
                                        <isLink>false</isLink>
                                    </featureRange>
                                </featureRangeList>
                            </feature>
                        </featureList>
                        <hostOrganismList>
                            <hostOrganism ncbiTaxId="83333">
                                <names>
                                    <shortLabel>ecoli</shortLabel>
                                    <fullName>Escherichia coli (strain K12)</fullName>
                                </names>
                            </hostOrganism>
                        </hostOrganismList>
                    </participant>
                    <participant id="181385">
                        <names>
                            <shortLabel>N/A</shortLabel>
                        </names>
                        <xref>
                            <primaryRef db="intact" dbAc="MI:0469" id="EBI-539735" refType="identity" refTypeAc="MI:0356"/>
                        </xref>
                        <interactorRef>181380</interactorRef>
                        <biologicalRole>
                            <names>
                                <shortLabel>unspecified role</shortLabel>
                                <fullName>unspecified role</fullName>
                            </names>
                            <xref>
                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                            </xref>
                        </biologicalRole>
                        <experimentalRoleList>
                            <experimentalRole>
                                <names>
                                    <shortLabel>prey</shortLabel>
                                    <fullName>prey</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                </xref>
                            </experimentalRole>
                        </experimentalRoleList>
                    </participant>
                </participantList>
                <interactionType>
                    <names>
                        <shortLabel>physical association</shortLabel>
                        <fullName>physical association</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactionType>
                <modelled>false</modelled>
                <intraMolecular>false</intraMolecular>
                <negative>false</negative>
            </interaction>
        </interactionList>
    </entry>
</entrySet>
