IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ leonoudakis-2004-5 Protein trafficking and anchoring complexes revealed by proteomic analysis of inward rectifier potassium channel (Kir2.x)-associated proteins. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Leonoudakis D., Conti LR., Anderson S., Radeke CM., McGuire LM., Adams ME., Froehner SC., Yates JR., Vandenberg CA. Synapse - Interactions of proteins with an established role in the presynapse. Detergent solubilized whole rat brain extract was used for affinity chromatography using a GST tagged bait (kir2.2). The interactors were eluted using a competitor bait peptide (ERPYRRESEI for kir2.2). Eluates were dried and analyzed by mass spectrometry after protein digestion. A control experiment using a Kir2.2 fragment with a 3 amino-acids deletion at the c-terminus was used instead. Only the interactors which were not found in the control experiment are reported here. table 1 vandenbe@lifesci.ucsb.edu J. Biol. Chem. (0021-9258) 2004 leonoudakis-2004-1 Protein trafficking and anchoring complexes revealed by proteomic analysis of inward rectifier potassium channel (Kir2.x)-associated proteins. in vitro In vitro pull down pull down fingerprinting peptide massfingerprinting fingerprinting Leonoudakis D., Conti LR., Anderson S., Radeke CM., McGuire LM., Adams ME., Froehner SC., Yates JR., Vandenberg CA. Synapse - Interactions of proteins with an established role in the presynapse. Detergent solubilized whole rat brain extract was used for affinity chromatography using a GST tagged bait (kir2.2). The interactors were eluted using a competitor bait peptide (ERPYRRESEI for kir2.2). Eluates were dried and analyzed by mass spectrometry after protein digestion. A control experiment using a Kir2.2 fragment with a 3 amino-acids deletion at the c-terminus was used instead. Only the interactors which were not found in the control experiment are reported here. table 1 vandenbe@lifesci.ucsb.edu J. Biol. Chem. (0021-9258) 2004 leonoudakis-2004-2 Protein trafficking and anchoring complexes revealed by proteomic analysis of inward rectifier potassium channel (Kir2.x)-associated proteins. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Leonoudakis D., Conti LR., Anderson S., Radeke CM., McGuire LM., Adams ME., Froehner SC., Yates JR., Vandenberg CA. Synapse - Interactions of proteins with an established role in the presynapse. Detergent solubilized whole rat brain extract was used for affinity chromatography using a GST tagged bait (kir2.2). The interactors were eluted using a competitor bait peptide (ERPYRRESEI for kir2.2). Eluates were dried and analyzed by mass spectrometry after protein digestion. A control experiment using a Kir2.2 fragment with a 3 amino-acids deletion at the c-terminus was used instead. Only the interactors which were not found in the control experiment are reported here. table 1 vandenbe@lifesci.ucsb.edu J. Biol. Chem. (0021-9258) 2004 leonoudakis-2004-3 Protein trafficking and anchoring complexes revealed by proteomic analysis of inward rectifier potassium channel (Kir2.x)-associated proteins. in vitro In vitro pull down pull down fingerprinting peptide massfingerprinting fingerprinting Leonoudakis D., Conti LR., Anderson S., Radeke CM., McGuire LM., Adams ME., Froehner SC., Yates JR., Vandenberg CA. Synapse - Interactions of proteins with an established role in the presynapse. Detergent solubilized whole rat brain extract was used for affinity chromatography using a GST tagged bait (kir2.2). The interactors were eluted using a competitor bait peptide (ERPYRRESEI for kir2.2). Eluates were dried and analyzed by mass spectrometry after protein digestion. A control experiment using a Kir2.2 fragment with a 3 amino-acids deletion at the c-terminus was used instead. Only the interactors which were not found in the control experiment are reported here. table 1 vandenbe@lifesci.ucsb.edu J. Biol. Chem. (0021-9258) 2004 leonoudakis-2004-4 Protein trafficking and anchoring complexes revealed by proteomic analysis of inward rectifier potassium channel (Kir2.x)-associated proteins. in vitro In vitro pull down pull down fingerprinting peptide massfingerprinting fingerprinting Leonoudakis D., Conti LR., Anderson S., Radeke CM., McGuire LM., Adams ME., Froehner SC., Yates JR., Vandenberg CA. Synapse - Interactions of proteins with an established role in the presynapse. Detergent solubilized whole rat brain extract was used for affinity chromatography using a GST tagged bait (kir2.2). The interactors were eluted using a competitor bait peptide (ERPYRRESEI for kir2.2). Eluates were dried and analyzed by mass spectrometry after protein digestion. A control experiment using a Kir2.2 fragment with a 3 amino-acids deletion at the c-terminus was used instead. Only the interactors which were not found in the control experiment are reported here. table 1 vandenbe@lifesci.ucsb.edu J. Biol. Chem. (0021-9258) 2004 cskp_rat Peripheral plasma membrane protein CASK Calcium/calmodulin-dependent serine protein kinase Cask protein protein rat Rattus norvegicus (Rat) Rat MADDDVLFEDVYELCEVIGKGPFSVVRRCINRETGQQFAVKIVDVAKFTSSPGLSTEDLKREASICHMLKHPHIVELLETYSSDGMLYMVFEFMDGADLCFEIVKRADAGFVYSEAVASHYMRQILEALRYCHDNNIIHRDVKPHCVLLASKENSAPVKLGGFGVAIQLGESGLVAGGRVGTPHFMAPEVVKREPYGKPVDVWGCGVILFILLSGCLPFYGTKERLFEGIIKGKYKMNPRQWSHISESAKDLVRRMLMLDPAERITVYEALNHPWLKERDRYAYKIHLPETVEQLRKFNARRKLKGAVLAAVSSHKFNSFYGDPPEELPDFSEDPTSSGLLAAERAVSQVLDSLEEIHALTDCSEKDLDFLHSVFQDQHLHTLLDLYDKINTKSSPQIRNPPSDAVQRAKEVLEEISCYPENNDAKELKRILTQPHFMALLQTHHVVAHEVYSDEALRVTPPPTSPYLNGDSPESANGDMDMENVTRVRLVQFQKNTDEPMGITLKMNELNHCIVARIMHGGMIHRQGTLHVGDEIREINGISVANQTVEQLQKMLREMRGSITFKIVPSYRTQSSSCERDSPSTSRQSPANGHSSTNNSVSIYVRAQFEYDPAKDDLIPCKEAGIRFRVGDIIQIISKDDHNWWQGKLENSKNGTAGLIPSPELQEWRVACIAMEKTKQEQQASCTWFGKKKKQYKDKYLAKHNADLVTYEEVVKLPAFKRKTLVLLGAHGVGRRHIKNTLITKHPERFAYPIPHTTRPPKKDEENGKNYYFVSHDQMMQDISNNEYLEYGSHEDAMYGTKLETIRKIHEQGLIAILDVEPQALKVLRTAEFPPFVVFIAAPTITPGLNEDESLQRLQKESDVLQRTYAHYFDLTIINNEIDETIRHLEEAVELVCTAPQWVPVSWVY lin7c_rat Protein lin-7 homolog C Mammalian lin-seven protein 3 Vertebrate lin-7 homolog 3 Lin7c Mals3 Veli3 protein protein rat Rattus norvegicus (Rat) Rat MAALGEPVRLERDICRAIELLEKLQRSGEVPPQKLQALQRVLQSEFCNAVREVYEHVYETVDISSSPEVRANATAKATVAAFAASEGHSHPRVVELPKTEEGLGFNIMGGKEQNSPIYISRIIPGGIADRHGGLKRGDQLLSVNGVSVEGEHHEKAVELLKAAQGKVKLVVRYTPKVLEEMESRFEKMRSAKRRQQT dlg1_rat Disks large homolog 1 Dlg1 Synapse-associated protein 97 Dlgh1 protein protein rat Rattus norvegicus (Rat) Rat MPVRKQDTQRALHLLEEYRSKLSQTEDRQLRSSIERVISIFQSNLFQALIDIQEFYEVTLLDNPKCVDHSKQCEPVQPGNPWESGSLSSAAVTSESLPGGLSPPVEKYRYQDEEVLPSERISPQVPNEVLGPELVHVSEKSLSEIENVHGFVSHSHISPIKPTEAVPPSSPIVPVTPALPVPAESPVVLPSTPQANPPPVLVNTDSLETPTYVNGTDADYEYEEITLERGNSGLGFSIAGGTDNPHIGDDSSIFITKIITGGAAAQDGRLRVNDCILRVNEADVRDVTHSKAVEALKEAGSIVRLYVKRRKAFRKNHEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGKLQIGDKLLAVNSVCLEEVTHEEAVTALKNTSDFVYLKAAKPTSMYINDGYAPPDITNSSSQSVDNHVSPSSYLGQTPASPARYSPISKAVLGDDEITREPRKVVLHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDRIISVNSVDLRAASHEQAAAALKNAGQAVTIVAQYRPEEYSRFEAKIHDLRETMMNSSVSSGSGSLRTSQKRSLYVRALFDYDKTKDSGLPSQGLNFKFGDILHVINASDDEWWQARQVTPDGESDEVGVIPSKRRVEKKERARLKTVKFNSKTRGDKGEIPDDMGSKGLKHVTSNASDSESSYHEYGCSKGGQEEYVLSYEPVNQQEVNYTRPVIILGPMKDRVNDDLISEFPDKFGSCVPHTTRPKRDYEVDGRDYHFVTSREQMEKDIQEHKFIEAGQYNNHLYGTSVQSVRAVAEKGKHCILDVSGNAIKRLQIAQLYPISIFIKPKSMENIMEMNKRLTDEQARKTFERAVRLEQEFTEHFTAIVQGDTLEDIYNQVKQIIEEQSGPYIWVPAKEKL kcj12_rat ATP-sensitive inward rectifier potassium channel 12 Potassium channel, inwardly rectifying subfamily J member 12 Inward rectifier K(+) channel Kir2.2 Kcnj12 Irk2 protein protein rat Rattus norvegicus (Rat) Rat MTAASRANPYSIVSSEEDGLHLVTMSGANGFGNGKVHTRRRCRNRFVKKNGQCNIEFANMDEKSQRYLADMFTTCVDIRWRYMLLIFSLAFLASWLLFGIIFWVIAVAHGDLEPAEGRGRTPCVLQVHGFMAAFLFSIETQTTIGYGLRCVTEECPVAVFMVVAQSIVGCIIDSFMIGAIMAKMGRPKKRAQTLLFSHNAVVALRDGKLCLMWRVGNLRKSHIVEAHVRAQLIKPRVTEEGEYIPLDQIDIDVGFDKGLDRIFLVSPITILHEIDEASPLFGISRQDLETDDFEIVVILEGMVEATAMTTQARSSYLANEILWGHRFEPVLFEEKNQYKIDYSHFHKTYEVPSTPRCSAKDLVENKFLLPSANSFCYENELAFLSRDEEDEVATDRDGRSPQPEHDFDRLQASSGALERPYRRESEI kcnj2_mouse Inward rectifier potassium channel 2 Potassium channel, inwardly rectifying subfamily J member 2 Inward rectifier K(+) channel Kir2.1 Kcnj2 Irk1 protein protein mouse Mus musculus Mouse MGSVRTNRYSIVSSEEDGMKLATMAVANGFGNGKSKVHTRQQCRSRFVKKDGHCNVQFINVGEKGQRYLADIFTTCVDIRWRWMLVIFCLAFVLSWLFFGCVFWLIALLHGDLDTSKVSKACVSEVNSFTAAFLFSIETQTTIGYGFRCVTDECPIAVFMVVFQSIVGCIIDAFIIGAVMAKMAKPKKRNETLVFSHNAVIAMRDGKLCLMWRVGNLRKSHLVEAHVRAQLLKSRITSEGEYIPLDQIDINVGFDSGIDRIFLVSPITIVHEIDEDSPLYDLSKQDIDNADFEIVVILEGMVEATAMTTQCRSSYLANEILWGHRYEPVLFEEKHYYKVDYSRFHKTYEVPNTPLCSARDLAEKKYILSNANSFCYENEVALTSKEEEEDSENGVPESTSTDSPPGIDLHNQASVPLEPRPLRRESEI kcnj4_human Inward rectifier potassium channel 4 Potassium channel, inwardly rectifying subfamily J member 4 Inward rectifier K(+) channel Kir2.3 Hippocampal inward rectifier HIRK2 HRK1 IRK3 KCNJ4 protein protein human Homo sapiens Human MHGHSRNGQAHVPRRKRRNRFVKKNGQCNVYFANLSNKSQRYMADIFTTCVDTRWRYMLMIFSAAFLVSWLFFGLLFWCIAFFHGDLEASPGVPAAGGPAAGGGGAAPVAPKPCIMHVNGFLGAFLFSVETQTTIGYGFRCVTEECPLAVIAVVVQSIVGCVIDSFMIGTIMAKMARPKKRAQTLLFSHHAVISVRDGKLCLMWRVGNLRKSHIVEAHVRAQLIKPYMTQEGEYLPLDQRDLNVGYDIGLDRIFLVSPIIIVHEIDEDSPLYGMGKEELESEDFEIVVILEGMVEATAMTTQARSSYLASEILWGHRFEPVVFEEKSHYKVDYSRFHKTYEVAGTPCCSARELQESKITVLPAPPPPPSAFCYENELALMSQEEEEMEEEAAAAAAVAAGLGLEAGSKEEAGIIRMLEFGSHLDLERMQASLPLDNISYRRESAI kcj10_rat ATP-sensitive inward rectifier potassium channel 10 Potassium channel, inwardly rectifying subfamily J member 10 ATP-sensitive inward rectifier potassium channel KAB-2 Inward rectifier K(+) channel Kir4.1 BIR10 Brain-specific inwardly rectifying K(+) channel 1 Kcnj10 Kab-2 protein protein rat Rattus norvegicus (Rat) Rat MTSVAKVYYSQTTQTESRPLVAPGIRRRRVLTKDGRSNVRMEHIADKRFLYLKDLWTTFIDMQWRYKLLLFSATFAGTWFLFGVVWYLVAVAHGDLLELGPPANHTPCVVQVHTLTGAFLFSLESQTTIGYGFRYISEECPLAIVLLIAQLVLTTILEIFITGTFLAKIARPKKRAETIRFSQHAVVAYHNGKLCLMIRVANMRKSLLIGCQVTGKLLQTHQTKEGENIRLNQVNVTFQVDTASDSPFLILPLTFYHVVDETSPLKDLPLRSGEGDFELVLILSGTVESTSATCQVRTSYLPEEILWGYEFTPAISLSASGKYVADFSLFDQVVKVASPGGLRDSTVRYGDPEKLKLEESLREQAEKEGSALSVRISNV dmd_rat Dystrophin Dmd protein protein rat Rattus norvegicus (Rat) Rat MLWWEEVEDCYEREDVQKKTFTKWINAQFSKFGKQHIDNLFSDLQDGKRLLDLLEGLTGQKLPKEKGSTRVHALNNVNKALQVLQKNNVDLVNIGSTDIVDGNHKLTLGLIWNIILHWQVKNVMKTIMAGLQQTNSEKILLSWVRESTRNYPQVNVLNFTSSWSDGLALNALIHSHRPDLFDWNSVVSQQSATQRLEHAFNIAKCQLGIEKLLDPEDVATTYPDKKSILMYITSLFQVLPQQVSIEAIREVEMLPRPSKVTREEHFQLHHQMHYSQQITVSLAQGYEQTSSSPKPRFKSYAFTQAAYVATSDSSQSPYPSQHLEAPGSKSFGSSLIETEVNLDSYQTALEEVLSWLLSAEDTLRAQGEISKDVEEVKEQFHAHEGFMMDLTSHQGLVGNVLQLGSRLVGKGKLTEDEETEVQEQMNLLNSRWECLRVASMEKQSNLHKVLMDLQNQKLKELDDWLTKTEERTKKMEEEPLGPDLEDLKCQVQQHKVLQEDLEQEQVRVNSLTHMVVVVDESSGDHATAALEEQLKVLGDRWANICKWTEDRWILLQDILLKWQRFTEEQCLFSKWLSEKEDAMKNIQTSGFEDQNEMVSSLQNISALKIDLEKKKQSMEKLSSLNQDLLSALKNKSVTQKMEMWMENFAQRWDNLTQKLEKSSAQISQAVTTTQPSLTQTTVMETVTMVTTREQIMVKHAQEELPPPPPQKKRQITVDSEIRKRLDVDITELHSWITRSEAVLQSSEFAVYRKEGNISDLKEKVNAIAREKAEKFRKLQDASRSAQALVEQMVNEGVNAESIRQASEQLNSRWTEFCQLLSERVNWLEYQNNIITFYNQLQQLEQMTTTAENLLKTQPTTLSEPTAIKSQLKICKDEVNRLSALQPQIERLKIQSLTLKEKGQGPMFLDADFVAFTNHFNYVFDGVRAREKELQTIFDTLPPMRYQETMSSIRTWIQQSENKLSIPHLSVTEYEIMEERLGKLQALQSSLKEQQNGFNYLNATVKEIAKKAPSEISQKYQSEFEEVEGRWKKLSTQLVEHCQKLEEHMNKLRKFQNHKKTLQKWMAEVDVFLKEEWPALGDAEILKKQLKQCRLLVGDIQTIQPSLNSVNEGGQKIKSEAEFEFASRLEKELKELNTQWDHICRQVYTRKEALKAGLDKTVSLQKDLSEMHEWMTQAEEEYLERDFEYKTPDELQTAVEEMKRAKEEALQKEAKVKLLTETVNSVISQAPPAAQEALKKELETLTTNYQWLCTRLNGKCKTLEEVWACWHELLSYLEKANKWLNEVELKLKATENVPAGAEEITEVLESLENLMHHSEENPNQIRLLAQTLTDGGVMDELINEELETFNSRWRELHEEAVRKQKLLEQSIQSAQEIEKSLHLIQESLEFIDKQLAAYIADKVDAAQMPQEAQKIQSDLTSHEISLEEMKKHNQGKDANQRVLSQIDVAQKKLQDVSIKFRLFQKPANFEQRLEESKMILDEVKMHLPALETKSVEQEVVQSQLSHCVNLYKSLSEVKSEVEMVIKTGRQIVQKKQTENPKELDERVTALKLHYNELGAKVTERKQQLEKCLKLSRKMRKEMNVLTEWLAATDTELTKRSAVEGMPSNLDSEVAWGKATQKEIEKQKAHLKSVTELGDSLKTVLGKKETLVEDKLTLLNSNWIAVTSRVEEWLNLLLEYQKHMESFDQNVEHITKWIIHTDELLDESEKRKPQQKEDILKRLKAEMNDIRPKVDATRDQAAKLMANRGDYCRKIVEPQISELNRRFAAISHRIKTGKASIPLKELEQFNSDIQKLLEPLEAEIQQGVNLKEEDFNKDMSEDNEGTVNELLQRGDNLQQRITDERKREEIKLKQQLLQTKHNALKDLRSQRRKKALEISHQWYQYKSQADDLLKCLDEIEKKLASLPEPRDERKIKEIDRELQKKKEELNAVRRQAESLSENGAAMAVEPTQIQLSKRWREIESNFAQFRRLNFAQIHTLHEETMVVTTEDMPLDVSYVPSTYLTEISHILQALSEVEQLLNAPELNAKDFEDLFKQEESLKNIKENLQQISGRIDVIHKKKTAALQSATPMERVKLQEAVSQMDFHWEKLNRMYKERQGRFDRSVEKWRHFHYDMKVFNQWLNDVEQFFKKTQNPENWEHAKYKWYLKELQDGIGQRQAVVRTLNATGEEIIQQSSKTDANILQEKLGSLSLRWHEVCKELAERRKRVEEQKNVFSEFQRDLNEFVSWLEEADNIATTPPGDEEQLKEKLEQVKLLTEELPLRQGILKQLNETGGAVLVSAPIRPEEQDKLEKKLKQTNLQWIKVSRALPEKQGELEVHIKDFRQFEEQLDHLLLWLSPIRNQLEIYNQPSQPGPFDLKETEVTVQAKQPDVERLLSKGQHLYKEKPSTQPVKRKLEDLRSEWEAVNHLLWELRTKQPDRAPGLSTTGASASQTVTVVTQPVDTKETVISKLEMPSSLLLEVPALADFNRAWTELTDWLSLLDRVIKSQRVMVGDLEDINEMIIKQKATLQDLEQRRPQLEELITAAQNLKNKTSNQEARTIITDRIERIQIQWDEVQEQLQNRRQQLNEMLKDSTQWLEAKEEAEQVIGQARGKLDSWKEGPHTMDAIQKKITETKQLAKDLRQRQINVDVANDLALKLLRDYSADDTRKVHMITENINTSWGNILKRVSEREAALEETQRLLQQFPLDLEKFLAWITEAETTANVLQDASRKEKLLEDSRGVRELMKPWQDLQGEIEAHTDIYHNLDENGQKILRSLEGSDEAPLLQRRLDNMNFKWSELRKKSLNIRSHLEVSSDQWKRLHLSLQELLVWLQLKDDELSRQAPIGGDFPAVQKQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELPPEERAQNVTRLLRKQAEEVNTEWDKLNLHSADWQRKIDEALERLQELQEAADELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPLKENVNHVNDLAHHLTTLGIQLSPYNLSILEDLNTRWRLLQVAVEDRVRQLHEAHRDFGPASQHFLSTSVQGPWERAISPNKVPYYINHETQTTCWDHPKMTELYQSLADLNNVRFSAYRTAMKLRRLQKALCLDLLSLSAACDALDQHNLKQNDQPMDILQIINCLTTIYDRLEQEHNNLVNVPLCVDMCLNWLLNVYDTGRTGRIRVLSFKTGIISLCKAHLEDKYRYLFKQVASSTGFCDQRRLGLLLHDSIQIPRQLGEVASFGGSNIEPSVRSCFQFANNKPEIEAALFLDWMRLEPQSMVWLPVLHRVAAAETAKHQAKCNICKECPIIGFRYRSLKHFNYDICQSCFFSGRVAKGHKMHYPMVEYCTPTTSGEDVRDFAKVLKNKFRTKRYFAKHPRMGYLPVQTVLEGDNMETPVTLINFWPVDSAPASSPQLSHDDTHSRIEHYASRLAEMENSNGSYLNDSISPNESIDDEHLLIQHYCQSLNQDSPLSQPRSPAQILISLESEERGELERILADLEEENRNLQAEYDRLKQQHEHKGLSPLPSPPEMMPTSPQSPRDAELIAEAKLLRQHKGRLEARMQILEDHNKQLESQLHRLRQLLEQPQAEAKVNGTTVSSPSTSLQRSDSSQPMLLRVVGSQTSESMGEEDLLSPPQDTSTGLEEVMEQLNNSFPSSRGRNAPGKPMREDTM lin7a_rat Protein lin-7 homolog A Mammalian lin-seven protein 1 Vertebrate lin-7 homolog 1 Lin7a Mals1 Veli1 protein protein rat Rattus norvegicus (Rat) Rat MLKPSVTSAPTADMATLTVVQPLTLDRDVARAIELLEKLQESGEVPVHKLQSLKKVLQSEFCTAIREVYQYMHETITVNGCPEFRARATAKATVAAFAASEGHSHPRVVELPKTDEGLGFNVMGGKEQNSPIYISRIIPGGVAERHGGLKRGDQLLSVNGVSVEGEHHEKAVELLKAAKDSVKLVVRYTPKVLEEMEARFEKLRTARRRQQQQLLIQQQQQQQQQPQQNHMS dlg2_rat Disks large homolog 2 Dlg2 Postsynaptic density protein PSD-93 Channel-associated protein of synapse-110 Dlgh2 protein protein rat Rattus norvegicus (Rat) Rat MFFACYCALRTNVKKYRYQDEDGPHDHSLPRLTHEVRGPELVHVSEKNLSQIENVHGYVLQSHISPLKASPAPIIVNTDTLDTIPYVNGTEIEYEFEEITLERGNSGLGFSIAGGTDNPHIGDDPGIFITKIIPGGAAAEDGRLRVNDCILRVNEVDVSEVSHSKAVEALKEAGSIVRLYVRRRRPILETVVEIKLFKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIDGGAAQKDGRLQVGDRLLMVNNYSLEEVTHEEAVAILKNTSDVVYLKVGKPTTIYMTDPYGPPDITHSYSPPMENHLLSGNNGTLEYKTSLPPISPGRYSPIPKHMLVEDDYTRPPEPVYSTVNKLCDKPASPRHYSPVECDKSFLLSTPYPHYHLGLLPDSDMTSHSQHSTATRQPSVTLQRAISLEGEPRKVVLHKGSTGLGFNIVGGEDGEGIFVSFILAGGPADLSGELQRGDQILSVNGIDLRGASHEQAAAALKGAGQTVTIIAQYQPEDYARFEAKIHDLREQMMNHSMSSGSGSLRTNQKRSLYVRAMFDYDKSKDSGLPSQGLSFKYGDILHVINASDDEWWQARRVILDGDSEEMGVIPSKRRVERKERARLKTVKFNAKPGVIDSKGDIPGLGDDGYGTKTLRGQEDLILSYEPVTRQEINYTRPVIILGPMKDRINDDLISEFPDKFGSCVPHTTRPKRDYEVDGRDYHFVISREQMEKDIQEHKFIEAGQYNDNLYGTSVQSVRFVAERGKHCILDVSGNAIKRLQVAQLYPIAIFIKPKSLEPLMEMNKRLTEEQAKKTYDRAIKLEQEFGEYFTAIVQGDTLEDIYNQCKLVIEEQSGPFIWIPSKEKL dlg3_rat Disks large homolog 3 Dlg3 Dlgh3 Synapse-associated protein 102 PSD-95/SAP90-related protein 1 protein protein rat Rattus norvegicus (Rat) Rat MHKHQHCCKCPECYEVTRLAALRRLEPPGYGDWQVPDPYGPSGGNGASSGYGGYSSQTLPSQAGATPTPRTKAKLIPTGRDVGPVPPKPVPGKNTPKLNGSGPSWWPECTCTNRDWYEQASPAPLLVNPEALEPSLSVNGSDGMFKYEEIVLERGNSGLGFSIAGGIDNPHVPDDPGIFITKIIPGGAAAMDGRLGVNDCVLRVNEVDVSEVVHSRAVEALKEAGPVVRLVVRRRQPPPETIMEVNLLKGPKGLGFSIAGGIGNQHIPGDNSIYITKIIEGGAAQKDGRLQIGDRLLAVNNTNLQDVRHEEAVASLKNTSDMVYLKVAKPGSLHLNDMYAPPDYASTFTALADNHISHNSSLGYLGAVESKVTYPAPPQVPPTRYSPIPRHMLAEEDFTREPRKIILHKGSTGLGFNIVGGEDGEGIFVSFILAGGPADLSGELRRGDRILSVNGVNLRNATHEQAAAALKRAGQSVTIVAQYRPEEYSRFESKIHDLREQMMNSSMSSGSGSLRTSEKRSLYVRALFDYDRTRDSCLPSQGLSFSYGDILHVINASDDEWWQARLVTPHGESEQIGVIPSKKRVEKKERARLKTVKFHARTGMIESNRDFPGLSDDYYGAKNLKGVTSNTSDSESSSKGQEDAILSYEPVTRQEIHYARPVIILGPMKDRVNDDLISEFPHKFGSCVPHTTRPRRDNEVDGQDYHFVVSREQMEKDIQDNKFIEAGQFNDNLYGTSIQSVRAVAERGKHCILDVSGNAIKRLQQAQLYPIAIFIKPKSIEALMEMNRRQTYEQANKIFDKAMKLEQEFGEYFTAIVQGDSLEEIYNKIKQIIEDQSGHYIWVPSPEKL dlg4_rat Disks large homolog 4 Dlg4 Dlgh4 Postsynaptic density protein 95 Synapse-associated protein 90 Psd95 protein protein rat Rattus norvegicus (Rat) Rat MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKVMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVIDAGDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL apba1_rat Amyloid-beta A4 precursor protein-binding family A member 1 Neuron-specific X11 protein Neuronal Munc18-1-interacting protein 1 Adapter protein X11alpha Apba1 Mint1 X11 protein protein rat Rattus norvegicus (Rat) Rat MNHLEGSAEVEVADEAPGGEVNESVEADLEHPEVEEEQQPSPPPPAGHAPEDHRAHPAPPPPPPPQEEEEERGECLARSASTESGFHNHTDTAEGDVLAAARDGYEAERAQDADDESAYAVQYRPEAEEYTEQAEAEHAEAAQRRALPNHLHFHSLEHEEAMNAAYSGYVYTHRLFHRAEDEPYAEPYADYGGLQEHVYEEIGDAPELEARDGLRLYERERDEAAAYRQEALGARLHHYDERSDGESDSPEKEAEFAPYPRMDSYEQEEDIDQIVAEVKQSMSSQSLDKAAEDMPEAEQDLERAPTPGGGHPDSPGLPAPAGQQQRVVGTPGGSEVGQRYSKEKRDAISLAIKDIKEAIEEVKTRTIRSPYTPDEPKEPIWVMRQDISPTRDCDDQRPVDGDSPSPGSSSPLGAESSITPLHPGDPTEASTNKESRKSLASFPTYVEVPGPCDPEDLIDGIIFAANYLGSTQLLSDKTPSKNVRMMQAQEAVSRIKTAQKLAKSRKKAPEGESQPMTEVDLFISTQRIKVLNADTQEPMMDHPLRTISYIADIGNIVVLMARRRMPRSNSQENVEASHPSQDAKRQYKMICHVFESEDAQLIAQSIGQAFSVAYQEFLRANGINPEDLSQKEYSDLLNTQDMYNDDLIHFSKSENCKDVFIEKQKGEILGVVIVESGWGSILPTVIIANMMHGGPAEKSGKLNIGDQIMSINGTSLVGLPLSTCQSIIKGLKNQSRVKLNIVRCPPVTTVLIRRPDLRYQLGFSVQNGIICSLMRGGIAERGGVRVGHRIIEINGQSVVATPHEKIVHILSNAVGEIHMKTMPAAMYRLLTAQEQPVYI kcnj12-dlg1-5 pull-down: bait= rat kir2.2 234108 N/A 234113 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] N/A 234114 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] N/A 234115 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] N/A 234116 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false pdz binding motif required to bind necessary binding region required to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) association association false false false rat dystrophins dp71, alpha-dystrobrevin-2 and synthrophins proteins were also identified kcnj2-dlg1-6 pull-down: bait= mouse kir2.1 234108 N/A 234117 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 234113 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] N/A 234114 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] N/A 234115 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] association association false false false kcnj4-dlg1-7 pull-down: bait= human kir2.3 234108 N/A 234113 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] N/A 234118 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminus sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 234115 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] N/A 234114 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] association association false false false kcnj4-dlg1-8 pull-down: bait= rat kir4.1 234108 N/A 234119 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 234120 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] physical association physical association false false false Rat dystrophin dp71, alpha-dystrobrevin-2 and synthrophins proteins were also identified kcnj12-dlg1-1 pull-down: bait= rat kir2.2 234109 N/A 234113 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 234114 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 234121 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 234122 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 234123 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 234124 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 234115 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 234116 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminus sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false pdz binding motif required to bind necessary binding region required to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) association association false false false rat Pals2 (Vam-1) and actin-binding LIM protein-1 (ab-LIM) were also identified kcnj12-dlg1-2 pull-down: bait= rat kir2.2 234110 N/A 234125 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 234123 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 234113 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 234114 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 234121 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 234122 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 234124 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 234115 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 234116 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false pdz binding motif required to bind necessary binding region required to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) association association false false false rat Pals2 (Vam-1), actin-binding LIM protein-1 (ab-LIM), alpha dystrobrevin-1, alpha-dystrobrevin-2 and dystrophins proteins were also identified kcnj12-dlg1-3 pull-down using rat heart extract : bait= rat kir2.2 234111 N/A 234125 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] N/A 234113 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] N/A 234114 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] N/A 234121 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] N/A 234115 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] N/A 234116 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false pdz binding motif required to bind necessary binding region required to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) association association false false false rat alpha1-syntrophin and beta1-syntrophin were also identified kcnj12-dlg1-4 pull-down using rat heart extract eluted with SDS: bait= rat kir2.2 234112 N/A 234115 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] N/A 234113 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] N/A 234114 unspecified role unspecified role prey prey rat-heart Rattus norvegicus heart heart Heart [Cardiac; Cardiac tissue] N/A 234116 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false pdz binding motif required to bind necessary binding region required to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) association association false false false rat dystrobrevin alpha2, dystrophin Dp427 and beta-syntrophin were also identified