IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ walther-2004-1 Functional dissection of the interactions of stonin 2 with the adaptor complex AP-2 and synaptotagmin. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Walther K., Diril MK., Jung N., Haucke V. Synapse - Interactions of proteins with an established role in the presynapse. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2004 walther-2004-2 Functional dissection of the interactions of stonin 2 with the adaptor complex AP-2 and synaptotagmin. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Walther K., Diril MK., Jung N., Haucke V. Synapse - Interactions of proteins with an established role in the presynapse. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2004 walther-2004-3 Functional dissection of the interactions of stonin 2 with the adaptor complex AP-2 and synaptotagmin. mouse-n1e-115 Mus musculus neuroblastoma cells n1e-115 Mouse neuroblastoma imaging technique imaging technique microscopy immunostaining immunostaining Walther K., Diril MK., Jung N., Haucke V. Synapse - Interactions of proteins with an established role in the presynapse. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2004 ap2m1_rat AP-2 complex subunit mu Adaptor protein complex AP-2 subunit mu AP-2 mu chain Ap2m1 Mu2-adaptin Plasma membrane adaptor AP-2 50 kDa protein Clathrin coat assembly protein AP50 Clathrin coat-associated protein AP50 Clathrin assembly protein complex 2 mu medium chain Adaptor-related protein complex 2 subunit mu protein protein rat Rattus norvegicus (Rat) Rat MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC peptide-wvtf tetrapeptide with the sequence WVTF protein protein in vitro In vitro WVTF AP2 mu2 binding site found in : stonin 1 (mouse, human), stonin 2 (mouse, human), stonin (fugu, zebrafish, xenopus tropicalus), synaptojanin 1 (human)... The more general motif 'WVxF' is also found in : intersectin 2, pacsin/syndapin 1, AAK1, NECAP1 human proteins ap2a2_rat AP-2 complex subunit alpha-2 Adaptor protein complex AP-2 subunit alpha-2 Alpha2-adaptin Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit Alpha-adaptin C Clathrin assembly protein complex 2 alpha-C large chain 100 kDa coated vesicle protein C Ap2a2 Adtab Adaptor-related protein complex 2 subunit alpha-2 protein protein rat Rattus norvegicus (Rat) Rat MPAVSKGEGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMGLALHCIANVGSREMAEAFAGEIPKILVAGDTMDSVKQSAALCLLRLYRTSPDLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQKNPEEFKTSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPDPAVRGRLTECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAQQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFNLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKATIQDVLRSDSQLKNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEETKRERSIDVNGGPEPVPASTSAASTPSPSADLLGLGAVPPAPTGPPPSSGGGLLVDVFSDSASAVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICADDLQTNLNLQTKPVDPTVDGGAQVQQVINIECISDFTEAPVLNIQFRYGGTFQNVSVKLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEITKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKDTVSQRLCELLSEQF ston2_mouse Stonin-2 Stoned B Ston2 Stnb Stn2 protein protein mouse Mus musculus Mouse MTTLDHVIATHQSEWVSFSEEPLFPTPLEGGTEEHFPGLSSSSERSESSSGENHVVDEGSQDLSHSEQDDSSEKMGLISEAASPPGSPVQPTPDLASAISNWVQFEDDTPWSSTSPPHKETALTLTMPCWTCPSFDSLRRCPLTSESSWTTHSEDTSSPSVAPSYTDLQLINTEEQASGRASGTDSTDNSSSLQEDEEVEMEAISWWAGSPAMNGHPAAPPVTTARFPSWVTFEDNEVGCPSPPVPSPKKPNTPSAATAAPDVPFNSTGSFKRDRPKSTLMNLPKVQKLDISSLNRPPSVIEAPPWRATNPFLNETLQDVQPSPINPFSAFFEEQERRSQNSSVSSTTGKSQRDSLIVVYQDAISFDDSGKSQPHPDAIEKLKQLQIDDPDPVGNTALPDDDPTASVELDAPSPASALSQPRDGWPMMLRIPEKKNIMSSRHWGPIYIKLTASGYLQLYYEQGLEKPFREFKLEICHEVSEPRLQNYDENGRIHSLRIDRVTYKEKKKYQPKPAVAHAAEREQVIKLGTTNYDDFRSFIHAVQDRLMDLPVLSMDLSTVGLNYLEEEITVDVRDEFSGTVGKGDNQILQHHVLTRIHILSFLSGLAECRLGLNDILIKGNEIVSRQDIMPTTTTKWIKLHECRFHGCVDEDVFNSSRVILFNPLDACRFELMRFRTVFAEKTLPFTLRTAASINGAEVEVQSWLRMSPGFSSNRDPLTQVPCENVMVRYPVPSEWVKNFRRDSVLGEKSLKAKVNRGASFGSAGASGSEPVMRVTLGTAKYEHAFNSIVWRINRLPDKNSASGHPHCFFCHLELGSDREVPSRFANYVNVEFSMPTTSASKAAVRSVSVEDKPDVRKWVNYSAHYSYKVEIEQKKSLKPDFEGEDLENPKECGVQ syt1_rat Synaptotagmin-1 Syt1 Synaptotagmin I p65 protein protein rat Rattus norvegicus (Rat) Rat MVSASHPEALAAPVTTVATLVPHNATEPASPGEGKEDAFSKLKQKFMNELHKIPLPPWALIAIAIVAVLLVVTCCFCVCKKCLFKKKNKKKGKEKGGKNAINMKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAVKK ston1_mouse Stonin-1 Stoned B-like factor Ston1 Sblf Stn1 Salf protein protein mouse Mus musculus Mouse MYSTNPGSWVTFDDDPAFQSSQKRKDFSLETQGVCRPNGLKLTLPTLRDPPSTPSSASSTPLSSPMVDFYFSPGPPSNSPLSTPTKDFPGFPGIPKAGTHVLYPIPECSSSSAPTTAGGVGPPLLLTKPDCSPHVSLPSSHSHTQPTPTLGFTEDAGPQRVQSEARQFEYFQDHCAFSNPFWKDEGSASPFPLDSLASRKPFSPKDKEVPIGHKSLTQCSLDYICEKLEHLHSAETQDPLGDLSMQDPYAGDTVSFVPHSLFRSQPRAGWSFMLRIPEKKNMMSSRQWGPIFLKVLPGGILQMYYEKGLEKPFKEFQLDPHCRLSEPKLENFSMAGKIHTVKVEHVSYSEKRKYHAKTEVVHEPEVEQMLKLGSTEHRDFLEFLTTVEEELIKLPATAKPKNKSYEEQEICLDIQDSLWGKVTKEGQLVESAVVTQICCLCFLNGPAECFLALNDRELQKRDECYFEKEPEKKGIAILDYHFHTCVKAEEFEQSRIIKFVPLDACRFELMRFKTSYEAGELPFAVKSVVTVQGAYVELQAFVNMTPAAQGSPHAGALRSCNNIMIHFPVPAQWIKALWTRNLQRQKSLKAKMNRRACLGSLQEPESEPVIQVTVGSAKYESAYRAVVWKIDRLPDKNSSPDQPHCLSYKLELGSDQEVPSDWYPFATVQFSMLEACASRTEVRSLGVESDAQPQKHVCQRACYNIQVEIEKKWIQVDGEDADKTGGCVTQ syt1_human Synaptotagmin-1 Synaptotagmin I p65 SYT1 SYT SVP65 protein protein human Homo sapiens Human MVSESHHEALAAPPVTTVATVLPSNATEPASPGEGKEDAFSKLKEKFMNELHKIPLPPWALIAIAIVAVLLVLTCCFCICKKCLFKKKNKKKGKEKGGKNAINMKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAVKK ston2_human Stonin-2 Stoned B STON2 STNB STN2 protein protein human Homo sapiens Human MTTLDHVIATHQSEWVSFNEEPPFPAHSQGGTEEHLPGLSSSPDQSESSSGENHVVDGGSQDHSHSEQDDSSEKMGLISEAASPPGSPEQPPPDLASAISNWVQFEDDTPWASTSPPHQETAETALPLTMPCWTCPSFDSLGRCPLTSESSWTTHSEDTSSPSFGCSYTDLQLINAEEQTSGQASGADSTDNSSSLQEDEEVEMEAISWQASSPAMNGHPAPPVTSARFPSWVTFDDNEVSCPLPPVTSPLKPNTPPSASVIPDVPYNSMGSFKKRDRPKSTLMNFSKVQKLDISSLNRTPSVTEASPWRATNPFLNETLQDVQPSPINPFSAFFEEQERRSQNSSISSTTGKSQRDSLIVIYQDAISFDDSSKTQSHSDAVEKLKQLQIDDPDHFGSATLPDDDPVAWIELDAHPPGSARSQPRDGWPMMLRIPEKKNIMSSRHWGPIFVKLTDTGYLQLYYEQGLEKPFREFKLEICHEISEPRLQNYDENGRIHSLRIDRVTYKEKKKYQPKPAVAHTAEREQVIKLGTTNYDDFLSFIHAVQDRLMDLPVLSMDLSTVGLNYLEEEITVDVRDEFSGIVSKGDNQILQHHVLTRIHILSFLSGLAECRLGLNDILVKGNEIVLRQDIMPTTTTKWIKLHECRFHGCVDEDVFHNSRVILFNPLDACRFELMRFRTVFAEKTLPFTLRTATSVNGAEVEVQSWLRMSTGFSANRDPLTQVPCENVMIRYPVPSEWVKNFRRESVLGEKSLKAKVNRGASFGSTSVSGSEPVMRVTLGTAKYEHAFNSIVWRINRLPDKNSASGHPHCFFCHLELGSDREVPSRFANHVNVEFSMPTTSASKASVRSISVEDKTDVRKWVNYSAHYSYQVALGSIWLMLPTPFVHPTTLPLLFLLAMLTMFAW ap2m1-wvtf-1 interaction between AP-2 mu2 adaptin and the WVTF tetrapeptide (found in stonin 2) 181286 N/A 181289 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false P84092:p.Trp421Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 181290 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false trp1ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false phe4ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false the 158-435 fragment of human, mouse and rat mu2 adaptin have the same protein sequence. ap2m1-wvtf-2 interaction between AP-2 mu2 adaptin and the WVTF tetrapeptide (found in stonin 2) 181286 N/A 181290 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false trp1ala,phe4ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 181289 unspecified role unspecified role prey prey in vitro In vitro physical association physical association false false false ap2a2-wvtf-1 interaction between AP-2 alpha c adaptin and the WVTF tetrapeptide (found in stonin 2) 181286 N/A 181291 unspecified role unspecified role prey prey in vitro In vitro N/A 181290 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false trp1ala phe4ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false ap2m1-stn2-2 interaction between synaptotatgmin and stonin 2 181286 N/A 181292 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 181293 unspecified role unspecified role prey prey rat-brain Rattus norvegicus brain brain Brain [cerebrum] physical association physical association false false false ap2m1-stn2-1 interaction between AP-2 mu2 adaptin and stonin 2 181287 N/A 181289 unspecified role unspecified role prey prey 35s radiolabel radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 181292 unspecified role unspecified role bait bait n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Stonin 2 WVTF motif is sufficient for AP2 mu2 binding ap2a2-stn2-1 interaction between AP-2 alpha c adaptin and stonin 2 181287 N/A 181292 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 181291 unspecified role unspecified role prey prey 35s radiolabel radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false stonin 2 WVTF motif is sufficient for AP2 alpha c binding ap2m1-salf-1 interaction between AP-2 mu2 adaptin and stonin 1 181287 N/A 181294 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 181289 unspecified role unspecified role prey prey 35s radiolabel binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rat-brain Rattus norvegicus brain brain Brain [cerebrum] physical association physical association false false false stn2-syt1-1 Synaptotagmin I recruits stonin 2 at the plasma membrane 181288 N/A 181295 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 181296 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false