IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ phee-2006-1 Identification of phytochrome-interacting protein candidates in Arabidopsis thaliana by co-immunoprecipitation coupled with MALDI-TOF MS. arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Phee BK., Shin DH., Cho JH., Kim SH., Kim JI., Lee YH., Jeon JS., Bhoo SH., Hahn TR. NDPK - Interactions involving proteins containing InterPro domain IPR001564, Nucleoside diphosphate kinase, core. Transgenic PhyA recessive arabidopsis mutants were engineered to express epitope (myc) tagged potato PhyA or potato PhyB. Note details of constructs used to achieve this are not given in the report. Table 1 Uniprot accession numbers were mapped using TAIR indexes of uniprot , ncbi and protein identifier numbers and manually where mappings were not available. shbhoo@khu.ac.kr Proteomics (1615-9853) 2006 1446..1449 1446..1459 2006-07-28: Only interaction involving solely Proteins were exported. Only protein-protein interactions 4 imex curation phee-2006-2 Identification of phytochrome-interacting protein candidates in Arabidopsis thaliana by co-immunoprecipitation coupled with MALDI-TOF MS. arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Phee BK., Shin DH., Cho JH., Kim SH., Kim JI., Lee YH., Jeon JS., Bhoo SH., Hahn TR. NDPK - Interactions involving proteins containing InterPro domain IPR001564, Nucleoside diphosphate kinase, core. Transgenic PhyA recessive arabidopsis mutants were engineered to express epitope (myc) tagged potato PhyA or potato PhyB. Note details of constructs used to achieve this are not given in the report. Figure 3 shbhoo@khu.ac.kr Proteomics (1615-9853) 2006 1446..1449 1446..1459 2006-07-28: Only interaction involving solely Proteins were exported. Only protein-protein interactions 4 imex curation atca4_arath Alpha carbonic anhydrase 4 ACA4 Alpha carbonate dehydratase 4 T13K14.150 At4g20990 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDTNAKTIFFMAMCFIYLSFPNISHAHSEVDDETPFTYEQKTEKGPEGWGKINPHWKVCNTGRYQSPIDLTNERVSLIHDQAWTRQYKPAPAVITNRGHDIMVSWKGDAGKMTIRKTDFNLVQCHWHSPSEHTVNGTRYDLELHMVHTSARGRTAVIGVLYKLGEPNEFLTKLLNGIKAVGNKEINLGMIDPREIRFQTRKFYRYIGSLTVPPCTEGVIWTVVKRVNTISMEQITALRQAVDDGFETNSRPVQDSKGRSVWFYDPNV q8guh3_arath Histone H2A protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSGRGKGGKAKTKGKSRSSRAGLQFPVGRIHRLLRKGNYAERVGAGAPVYLAAVMEYLAAEVLELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLSGVTIAQGGVLPNIQAVLLPKKTSAPVTSSAPKSGKKSSQSQEY rogfd_arath Rop guanine nucleotide exchange factor 13 ROPGEF13 PIRF2 Phytochrome interacting RopGEF 2 Rho of plants guanine nucleotide exchange factor 13 MSL1.17 At3g16130 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVKASEKEHEKYKPKLFDLENVKKKNSSSRHFKRWNSDSALRIEDPDIDDGTVFKKTATSSIQPILPVLAMDEQPQPREATDEESQKDSGKREIISFLGYNEKFTRDQVILRFRGLQAKSCYFAYVTELEQMKDKFAKLLLGEDMSGGSKGVSSALALSNAITNLAASAFGEIRRLEAISEDKKERWRREIGWLLSVTDHIVEFSPTHQTNEDGSSMEVMTTKQRTDLVSNIPSLKKLDEMLLDCLDKFKDQDEFYYVTPGSPESENSNSTRNDDKWWLPIVKVPPKGLSETLKRFLLSQRECVCQVLNSAMAINSQVLTEMEIPESYIDSLPKKGRASLGDMIYRMITLEMFDAEQFLLEMDLSSEHKILDLKNKFEASVVIWQRKIVQIDNKSSSPWSTNLSMDKRQQLEERAATILQLIKQEFPGISQSTLDISKIQFNRDIGLAIVESYSRILESLAHTVMSRIEDVLEADQLTQNPELAMCKIHIVKETESPEKEEEPNFCLLEDRPKKQKPTISLSEVMQWNIETNEPRKEKSDKKLLTRVSSMIMSNNKKTTYLESLGTTRSPTAGRYS thoc6_arath THO complex subunit 6 THO6 DWA1 Protein DWD HYPERSENSITIVE TO ABA 1 THOC6 WD repeat-containing protein DWA1 F27F23.25 At2g19430 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MYGDATNWNEDEYRESILKEREIETRTVFRTAWAPPARISNPDAFVVASSDGTLAFHSLNSLVSQSASFGYSKGQDVMVAEPERVVRAHEGPAYDVKFYGEDEDALLLSCGDDGRVRGWKWREFAESDVSLHLKENHLKPLLELINPQHKGPWGALSPMPEINAMSVDPQSGSVFTAAGDSCAYCWDVESGKIKMTFKGHSDYLHTVVSRSSASQILTGSEDGTARIWDCKTGKCVKVIGSQDKKSRLRVSSMALDGSESWLVCGQGKNLALWNLPASECVQTIPIPAHVQDVMFDEKQILTVGAEPLLRRFDLNGALLSQIHCAPCSVFSISLHPAGVVAVGGYGGIVDVISQFGSHLCTFRSSSL pia1_arath Phytochrome-interacting ankyrin-repeat protein 1 PIA1 F13G24.40 MXM12.8 At5g07840 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLQEQPVALSFRPNSFRRRSMETGVDRDDRGWTQLHIKAREGDLKAVKELLDQGADVNALACGPKSKGMTPLHLAAKGGHIEVMDLLLERGANMEARTSGACGWTPLHAAAKERKREAVKFLVGNGAFLPDDITDSRFNPPVQYCHGLEWAYEERKKLSEDTSLSCGDTSCSSAN p2c09_arath Probable protein phosphatase 2C 9 Phytochrome-associated protein phosphatase 2C T16E15.10 At1g22280 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGKFCCFTSASEVVGGQSSSRSGKGRSDEGMIKYGFSLVKGKANHPMEDYHVANFINIQDHELGLFAIYDGHMGDSVPAYLQKRLFSNILKEGEFWVDPRRSIAKAYEKTDQAILSNSSDLGRGGSTAVTAILINGRKLWIANVGDSRAVLSHGGAITQMSTDHEPRTERSSIEDRGGFVSNLPGDVPRVNGQLAVSRAFGDKGLKTHLSSEPDIKEATVDSQTDVLLLASDGIWKVMTNEEAMEIARRVKDPQKAAKELTAEALRRESKDDISCVVVRFR naca2_arath Nascent polypeptide-associated complex subunit alpha-like protein 2 At3g49470 T9C5.70 Alpha-NAC-like protein 2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSPPPAVVTESADGQPEQPPVTAIAEELEKKLQTDEPIVEDVKDDEDDDDDDEEEEDDDAQGVSGSSKQSRSEKKSRKAMLKLGMKPVTGVSRVTIKRTKNVLFFISKPDVFKSPHSETYVIFGEAKIEDLSSQLQTQAAQQFRMPEIGATSQRAEASTATVEAQVEEDEEEIDETGVEARDIDLVMTQAGVSRSKAVKALKSHDGDIVSAIMELTT phya_soltu Phytochrome A PHYA protein protein soltu Solanum tuberosum (Potato) Potato MSSSRPSQSSTTSSRSKHSARIIAQTSIDAKLHADFEESGDSFDYSSSVRVTNVAEGEQRPKSDKVTTAYLHQIQKGKFIQPFGCLLALDEKTLKVIAFSENAPEMLTMVSHAVPSVGEHPVLGIGIDIRTIFTGPSGAALQKALGFGEVSLLNPVLVHCKNSGKPFYAIVHRVTGSLIIDFEPVKPYEVPMTAAGALQSYKLAAKAITRLQSLPSGSMERLCDTMVQEVFELTGYDRVMGYKFHDDDHGEVVSEITKPGLEPYLGLHYPATDIPQAARFLFMKNKVRMICDCRAKHVKVVQDEKLPFDLTLCGSTLRAPHYCHLQYMENMNSIASLVMAVVVNDGDEEGESSDSSQSQKRKRLWGLVVSHNTTPRFAPFPLRYACEFLAQVFAILVNKELELENQFLEKNILRTQTLLCDMLMRDAPLGIVSQSPNIMDLIKCDGAALLYKNKIHRLGMNPSDFQLHDIVSWLCEYHTDSTGLSTDSLYDAGFPGALALGDAVCGMAAVRISDKDWLFWYRSHTAAEVRWGGAKHEPGEKDDGRKMHPRSSFKGFLEVVKTRSIPWKDYEMDRIHSLQLILRNAFKDADAVNSNTISIHTKLNDLKIDGMQELEAVTAEMVRLIETASVPIFAVDVDGQVNGWNTKVAELTGLPVDEAIGKHLLTLVEDSSVDTVNKMLELALQGQEERNVEFEIKTHGPSRDSSPISLIVNACASKDVRDSVVGVCFIAQDITGQKSIMDKFTRIEGDYRAIIQNPHPLIPPIFGTDQFGWCSEWNSAMTMLTGWRRDDVMDKMLLGEVFGTQAACCRLKNQEAFVNFGVILNNAITGQESEKIPFGFFARYGKYVECLLCVSKRLDKEGAVTGLFCFLQLASHELQQALHVQRLSEQTALKRLKVLAYIRRQIRNPLSGIIFSRKMLEGTSLGEEQKNILHTSAQCQRQLDKILDDTDLDSIIEGYLDLEMLEFKLHEVLVASISQVMMKSNGKNIMISNDMVEDLLNETLYGDSPRLQQVLANFLLVSVNSTPSGGKLSISGKLTKDRIGESVQLALLEFRIRHTGGGVPEELLSQMFGSEADASEEGISLLVSRKLVKLMNGEVQYLREAGRSTFIISVELAVATKSS q8rxr8_arath F22D16.1 F22D16_1 At1g02990 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MFLALTQMLLSLTLMRNSKTSWDIFRKILKVEFLLKIWFGGYGSFLSMYQRSPVCSRPKTSPEVQQNQLGGRSNCSASSLVPQLSISGSASKPPASDVLVKLNKFVKSSHIGTPDSKHMSDAKTSSSAPSNHKTLRFRIKVGSSDLSSLKNVSTFTKEGLNMLPSASRVNCLSEVEQDLLNGICDSPTKILMAMVSFPLHKDQLLSPLSDDLIQLGSKEKILKDAGYGSTNKTDAKSTPDGLVVSDSQKRAGKFPIGKKEKLRDRVKYRPPSNKLDRNHTVSNTEKEADKESCEELVSKTMKLPLLSCLSPSYIHPAKEIDNVSDSNVESILRGTNKDAALMGSKPELEDNVVAFSDRSVKETESINVRKDVYLIKGEPLNSLESNPKREKAPSIEHVDYSSVVKGTQSETRNEEQILKSKLPKVQRSQKGSSSIVTMNSQRGKDAAVNIIKKNVPDKLQEDIEESEHMCKGFFGDSKESKEEKQISPVLKAEKEKLSEENALGESFNSVKNDEEACDHLNLVCEPDLKHLIKPSDLNEDRHTTKQSVRREVKNKHSLEGGMENMGMESERELSGVSKKPKTGKSRFSAVDQPGSNKSNQILEVLDTNKTMITQALAENVKDFAKASSHGERDDRKRKLKENEESGDCMRLREAAVMESSGENVRKRKRLKGSSCDEKELPFSSESCDKERSVSQENGRDSASHLPSTLSSPSLCKDLGSEIIKNNVRESKGSLVESVAPLALRVLDSGELKSGRISERDEYHDTDYNAGETLKRCRDGEAYSTIDRPGTTKKAAEDSKDRERAYGEDCSIENLKPKKSGRYPGENCIEGDSKQKSREEESSAPSKDNNWGLVNNVQDLGTAVKVKTKESRSKKRPARKVSMECNKEDSREYQDPNTKLDRSGSHFSSRQKPDTANTSRGKSNPLEVTTEQLKNKSASPAQVEVLGHDTEISNTKKQRLRNDNHSVTHDEGSRNQKQNGSRHKDHVGLSPFKKESTSQTASNSIKEATDLKHMADRLKNAVSNHESTGVYFQAALKFLHGASLLESSGTTIARSKDIYGSTAKLCDQRN gun12_arath Endoglucanase 12 At2g44540 Endo-1,4-beta glucanase 12 F4I1.52 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSQSKNGSSQCLWTSICIVVLVMSMARGAVSTNYAEALQKSLLYFEAQRSGKLPPNQKVTWRGDSALKDGSDAHIDLTGGYYDAGDNMKFGFPLAFTTTMLAWSNIEMGSELRAHHEQGNSLRALKWATDYLIKAHPQPNVLYGQVGEGNSDHKCWMRPEDMTTPRTSYRIDAQHPGSDLAGETAAAMAAASIAFAPFDKAYANILIGHAKDLFAFAKAHPGLYQNSITNAGGFYASSGYEDELLWAAAWLHRATNDQIYLNYLTQASGTGGPRSVFAWDDKFVGAQVLMAKLALERKVGSNGKIAEYKSMAEQFICNCAQKGSNNVKKTPGGLLYFLPWNNLQYTTTASFVLSAYSKYLTKAKASIQCPKGALQASDLLQIARSQVDYILGSNPQKMSYMVGVGTNYPKKPHHRAASIVSIRKDKTPVTCSGGYDKWYNNPAPNPNVLMGALVGGPNENDVYGDERSNFQQAEPATVTVAPFVGVLAAVF rlk6_arath Receptor-like protein kinase At3g21340 Leucine-rich repeat receptor-like protein kinase At3g21340 MHC9.2 At3g21340 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEYHPQAIRLCALIFISFYALLHLVEAQDQKGFISLDCGSLPNEPPYNDPSTGLTYSTDDGFVQSGKTGRIQKAFESIFSKPSLKLRYFPDGFRNCYTLNVTQDTNYLIKAVFVYGNYDGLNNPPSFDLYLGPNLWVTVDMNGRTNGTIQEIIHKTISKSLQVCLVKTGTSSPMINTLELRPLKNNTYNTQSGSLKYFFRYYFSGSGQNIRYPDDVNDRKWYPFFDAKEWTELTTNLNINSSNGYAPPEVVMASASTPISTFGTWNFSWLLPSSTTQFYVYMHFAEIQTLRSLDTREFKVTLNGKLAYERYSPKTLATETIFYSTPQQCEDGTCLLELTKTPKSTLPPLMNALEVFTVIDFPQMETNPDDVAAIKSIQSTYGLSKISWQGDPCVPKQFLWEGLNCNNLDNSTPPIVTSLNLSSSHLTGIIAQGIQNLTHLQELDLSNNNLTGGIPEFLADIKSLLVINLSGNNFNGSIPQILLQKKGLKLILEGNANLICPDGLCVNKAGNGGAKKMNVVIPIVASVAFVVVLGSALAFFFIFKKKKTSNSQDLGPSSYTQVSEVRTIRSSESAIMTKNRRFTYSEVVTMTNNFERVLGKGGFGMVYHGTVNNTEQVAVKMLSHSSSQGYKEFKAEVELLLRVHHKNLVGLVGYCDEGENLALIYEYMANGDLREHMSGKRGGSILNWETRLKIVVESAQGLEYLHNGCKPPMVHRDVKTTNILLNEHLHAKLADFGLSRSFPIEGETHVSTVVAGTPGYLDPEYYRTNWLNEKSDVYSFGIVLLEIITNQLVINQSREKPHIAEWVGLMLTKGDIQNIMDPKLYGDYDSGSVWRAVELAMSCLNPSSARRPTMSQVVIELNECLSYENARGGTSQNMNSESSIEVSMNFDIGATPDAR phyb_soltu Phytochrome B PHYB protein protein soltu Solanum tuberosum (Potato) Potato MASGSRTKHSHHSSSQAQSSGTSNVNYKDSISKAIAQYTADARLHAVFEQSGESGKFFDYSQSVKTTTQSVPERQITAYLTKIQRGGHIQPFGCMIAVDEASFRVIAYSENACEMLSLTPQSVPSLEKCEILTIGTDVRTLFTPSSSVLLERAFGAREITLLNPIWIHSKNSGKPFYAILHRVDVGIVIDLEPARTEDPALSIAGAVQSQKLAVRAISHLQSLPGGDIKLLCDTVVESVRELTGYDRVMVYKFHEDEHGEVVAESKRSDLEPYIGLHYPATDIPQASRFLFKQNRVRMIVDCHATPVRVTQDESLMQPLCLVGSTLRAPHGCHAQYMANMGSIASLTLAVIINGNDEEAVGGGRNSMRLWGLVVGHHTSVRSIPFPLRYACEFLMQAFGLQLNMELQLASQLSEKHVLRTQTLLCDMLLRDSPPGIVTQSPSIMDLVKCDGAALYYQGKYYPLGVTPTEAQIKDIVEWLLAYHGDSTGLSTDSLPDAGYPGAASLGDAVCGMAVAYITSKDFLFWFRSHTAKEIKWGGAKHHPEDKDDGQRMHPRSSFKAFLEVVKSRSSPWENAEMDAIHSLQLILRDSFKDAEASNSKAIVHAHLGEMELQGIDELSSVAREMVRLIETATAPIFAVDVEGRINGWNAKVAELTGVSVEEAMGKSLVHDLVYKESQETAEKLLYNALRGEEDKNVEIKLRTFGAEQLEKAVFVVVNACASKDYTNNIVGVCFVGQDVTGEKVVMDKFINIQGDYKAIVHSPNPLIPPIFASDENTCCSEWNTAMEKLTGWSRGEIVGKMLVGEIFGSCCRLKGPDAMTKFMIVLHNAIGGQDTDKFPFSFFDRNGKYVQALLTANKRVNMEGDTIGAFCFIQIASPELQQALRVQRQQEKKCYSQMKELAYICQEIKSPLNGIRFTNSLLEATNLTENQKQYLETSAACERQMSKIIRDIDLENIEDGSLTLEKEDFFLGSVIDAVVSQVMLLLREKGVQLIRDIPEEIKTLTVHGDQVRIQQVLADFLLNMVRYAPSPDGWVEIQLRPSMMPISDGVTVVHIELRIICPGEGLPPELVQDMFHSSRWVTQEGLGLSMCRKMLKLMNGEIQYIRESERCYFLIILDLPMTRKGPKSVG ndk2_arath Nucleoside diphosphate kinase II, chloroplastic NDPK2 At5g63310 MDC12.28 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVGATVVSKWTPLCVASPPERNSASLNPHCSPARVNFRTALAAFRPQFRLFSRNSASRRRLRASSSAESGIFLPHLVASMEDVEETYIMVKPDGIQRGLVGEIISRFEKKGFKLIGLKMFQCPKELAEEHYKDLSAKSFFPNLIEYITSGPVVCMAWEGVGVVASARKLIGKTDPLQAEPGTIRGDLAVQTGRNIVHGSDSPENGKREIGLWFKEGELCKWDSALATWLRE pif3_arath Transcription factor PIF3 Phytochrome-associated protein 3 Basic helix-loop-helix protein 8 PIF3 PAP3 BHLH8 At1g09530 F14J9.19 Transcription factor EN 100 bHLH transcription factor bHLH008 Phytochrome-interacting factor 3 EN100 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MPLFELFRLTKAKLESAQDRNPSPPVDEVVELVWENGQISTQSQSSRSRNIPPPQANSSRAREIGNGSKTTMVDEIPMSVPSLMTGLSQDDDFVPWLNHHPSLDGYCSDFLRDVSSPVTVNEQESDMAVNQTAFPLFQRRKDGNESAPAASSSQYNGFQSHSLYGSDRARDLPSQQTNPDRFTQTQEPLITSNKPSLVNFSHFLRPATFAKTTNNNLHDTKEKSPQSPPNVFQTRVLGAKDSEDKVLNESVASATPKDNQKACLISEDSCRKDQESEKAVVCSSVGSGNSLDGPSESPSLSLKRKHSNIQDIDCHSEDVEEESGDGRKEAGPSRTGLGSKRSRSAEVHNLSERRRRDRINEKMRALQELIPNCNKVDKASMLDEAIEYLKSLQLQVQIMSMASGYYLPPAVMFPPGMGHYPAAAAAMAMGMGMPYAMGLPDLSRGGSSVNHGPQFQVSGMQQQPVAMGIPRVSGGGIFAGSSTIGNGSTRDLSGSKDQTTTNNNSNLKPIKRKQGSSDQFCGSS phya-naca2 PhyA interacting proteins 377313 N/A 377315 unspecified role unspecified role prey prey N/A 377316 unspecified role unspecified role prey prey N/A 377317 unspecified role unspecified role prey prey N/A 377318 unspecified role unspecified role prey prey N/A 377319 unspecified role unspecified role prey prey N/A 377320 unspecified role unspecified role prey prey N/A 377321 unspecified role unspecified role prey prey N/A 377322 unspecified role unspecified role bait bait n terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false phyb-naca2 PhyB interacting proteins 377313 N/A 377319 unspecified role unspecified role prey prey N/A 377323 unspecified role unspecified role prey prey N/A 377324 unspecified role unspecified role prey prey N/A 377320 unspecified role unspecified role prey prey N/A 377318 unspecified role unspecified role prey prey N/A 377315 unspecified role unspecified role prey prey N/A 377317 unspecified role unspecified role prey prey N/A 377321 unspecified role unspecified role prey prey N/A 377325 unspecified role unspecified role prey prey N/A 377326 unspecified role unspecified role bait bait n terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false phya-pif3 PHYA interacting proteins 377314 N/A 377327 unspecified role unspecified role prey prey N/A 377328 unspecified role unspecified role prey prey N/A 377322 unspecified role unspecified role bait bait n terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false phyb-pif3-4 PHYB interacting proteins 377314 N/A 377327 unspecified role unspecified role prey prey N/A 377326 unspecified role unspecified role bait bait n terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 377328 unspecified role unspecified role prey prey association association false false false