MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint anonymous-2014u-2 PSTPIP1 inhibition of TCR signalling depends on its SH3 domain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Rare diseases - Interactions investigated in the context of Rare genetic disease IBD - Inflammatory bowel disease andres@ibgm.uva.es 2014 imex curation Marcos T., Ruiz-Martín V., de la Puerta M.L., Trinidad A.G., Rodríguez M.d.e.l. .C., de la Fuente M.A., Sánchez Crespo M., Alonso A., Bayón Y. FEBS J. (1742-464X) Only protein-protein interactions anonymous-2014n-8 PSTPIP1 inhibition of TCR signalling depends on its SH3 domain human-jurkat Homo sapiens jurkat Human T cell leukemia anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Rare diseases - Interactions investigated in the context of Rare genetic disease IBD - Inflammatory bowel disease andres@ibgm.uva.es 2014 imex curation Marcos T., Ruiz-Martín V., de la Puerta M.L., Trinidad A.G., Rodríguez M.d.e.l. .C., de la Fuente M.A., Sánchez Crespo M., Alonso A., Bayón Y. FEBS J. (1742-464X) Only protein-protein interactions anonymous-2014u-3 PSTPIP1 inhibition of TCR signalling depends on its SH3 domain in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Rare diseases - Interactions investigated in the context of Rare genetic disease IBD - Inflammatory bowel disease andres@ibgm.uva.es 2014 imex curation Marcos T., Ruiz-Martín V., de la Puerta M.L., Trinidad A.G., Rodríguez M.d.e.l. .C., de la Fuente M.A., Sánchez Crespo M., Alonso A., Bayón Y. FEBS J. (1742-464X) Only protein-protein interactions anonymous-2014u-4 PSTPIP1 inhibition of TCR signalling depends on its SH3 domain human-jurkat Homo sapiens jurkat Human T cell leukemia anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Rare diseases - Interactions investigated in the context of Rare genetic disease IBD - Inflammatory bowel disease andres@ibgm.uva.es 2014 imex curation Marcos T., Ruiz-Martín V., de la Puerta M.L., Trinidad A.G., Rodríguez M.d.e.l. .C., de la Fuente M.A., Sánchez Crespo M., Alonso A., Bayón Y. FEBS J. (1742-464X) Only protein-protein interactions ppip1_human Proline-serine-threonine phosphatase-interacting protein 1 CD2-binding protein 1 H-PIP PSTPIP1 CD2BP1 protein protein human Homo sapiens Human MMPQLQFKDAFWCRDFTAHTGYEVLLQRLLDGRKMCKDMEELLRQRAQAEERYGKELVQIARKAGGQTEINSLRASFDSLKQQMENVGSSHIQLALTLREELRSLEEFRERQKEQRKKYEAVMDRVQKSKLSLYKKAMESKKTYEQKCRDADDAEQAFERISANGHQKQVEKSQNKARQCKDSATEAERVYRQSIAQLEKVRAEWEQEHRTTCEAFQLQEFDRLTILRNALWVHSNQLSMQCVKDDELYEEVRLTLEGCSIDADIDSFIQAKSTGTEPPAPVPYQNYYDREVTPLTSSPGIQPSCGMIKRFSGLLHGSPKTTSLAASAASTETLTPTPERNEGVYTAIAVQEIQGNPASPAQEYRALYDYTAQNPDELDLSAGDILEVILEGEDGWWTVERNGQRGFVPGSYLEKL ptn22_human Tyrosine-protein phosphatase non-receptor type 22 PTPN22 PTPN8 Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP Lymphoid phosphatase PEST-domain phosphatase protein protein human Homo sapiens Human MDQREILQKFLDEAQSKKITKEEFANEFLKLKRQSTKYKADKTYPTTVAEKPKNIKKNRYKDILPYDYSRVELSLITSDEDSSYINANFIKGVYGPKAYIATQGPLSTTLLDFWRMIWEYSVLIIVMACMEYEMGKKKCERYWAEPGEMQLEFGPFSVSCEAEKRKSDYIIRTLKVKFNSETRTIYQFHYKNWPDHDVPSSIDPILELIWDVRCYQEDDSVPICIHCSAGCGRTGVICAIDYTWMLLKDGIIPENFSVFSLIREMRTQRPSLVQTQEQYELVYNAVLELFKRQMDVIRDKHSGTESQAKHCIPEKNHTLQADSYSPNLPKSTTKAAKMMNQQRTKMEIKESSSFDFRTSEISAKEELVLHPAKSSTSFDFLELNYSFDKNADTTMKWQTKAFPIVGEPLQKHQSLDLGSLLFEGCSNSKPVNAAGRYFNSKVPITRTKSTPFELIQQRETKEVDSKENFSYLESQPHDSCFVEMQAQKVMHVSSAELNYSLPYDSKHQIRNASNVKHHDSSALGVYSYIPLVENPYFSSWPPSGTSSKMSLDLPEKQDGTVFPSSLLPTSSTSLFSYYNSHDSLSLNSPTNISSLLNQESAVLATAPRIDDEIPPPLPVRTPESFIVVEEAGEFSPNVPKSLSSAVKVKIGTSLEWGGTSEPKKFDDSVILRPSKSVKLRSPKSELHQDRSSPPPPLPERTLESFFLADEDCMQAQSIETYSTSYPDTMENSTSSKQTLKTPGKSFTRSKSLKILRNMKKSICNSCPPNKPAESVQSNNSSSFLNFGFANRFSKPKGPRNPPPTWNI pstpip1-ptpn22-2 1554360 n/a PSTPIP1 1554364 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tag egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a LYP 1554365 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false f3c pstpip1-ptpn22-4 1554360 n/a PSTPIP1 1554364 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell O43586:p.Trp232Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a LYP 1554365 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false f3d ptpn22-pstpip1 1554360 n/a PSTPIP1 1554364 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a LYP 1554365 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false f3e we deleted the CTH motif from LYP and observed that this deletion abrogated LYP/PSTPIP1 interaction (Figure 3E). Additionally, in this experiment we tested whether the two variants of LYP, LYP-R and LYP-W, which is associated to several autoimmune diseases [20], bind to PSTPIP1 and no difference was observed (Figure 3E). pstpip1-ptpn22-1 1554361 n/a LYP 1554365 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level n/a PSTPIP1 1554364 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false f3b we examined whether PSTPIP1 could bind to LYP by co-immunoprecipitation. PSTPIP1 was co-precipitated with LYP in PBLs and Jurkat cells (Figure 3B), ptpn22-pstpip1-1 1554362 n/a LYP 1554365 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait purified purified range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a PSTPIP1 1554364 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false f3f pstpip1-ptpn22-5 1554363 n/a PSTPIP1 1554364 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false O43586:p.Glu250Gln mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false n/a LYP 1554365 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false f5b