IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hedrich-2013-1 cAMP-responsive element modulator α (CREMα) trans-represses the transmembrane glycoprotein CD8 and contributes to the generation of CD3+CD4-CD8- T cells in health and disease. human-t-cell_cd8+ Homo sapiens CD8+ T lymphocytes t-cell_cd8+ CD8+ T lymphocytes ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Hedrich C.M., Rauen T., Crispin J.C., Koga T., Ioannidis C., Zajdel M., Kyttaris V.C., Tsokos G.C. IBD - Inflammatory bowel disease Binding of phospho-CREB to the CD8B promoter was also measured in this chromatin immunoprecipitation experiment; however, the identity of the CREB protein could not be established, hence this interaction is not captured here. chedrich@bidmc.harvard.edu,gtsokos@bidmc.harvard.edu J. Biol. Chem. (0021-9258) 2013 Only protein-protein interactions imex curation crem_human cAMP-responsive element modulator CREM Inducible cAMP early repressor protein protein human Homo sapiens Human MTMETVESQHDGSITASLTESKSAHVQTQTGQNSIPALAQVSVAGSGTRRGSPAVTLVQLPSGQTIHVQGVIQTPQPWVIQSSEIHTVQVAAIAETDESAESEGVIDSHKRREILSRRPSYRKILNELSSDVPGVPKIEEERSEEEGTPPSIATMAVPTSIYQTSTGQYIAIAQGGTIQISNPGSDGVQGLQALTMTNSGAPPPGATIVQYAAQSADGTQQFFVPGSQVVVQDEETELAPSHMAAATGDMPTYQIRAPTAALPQGVVMAASPGSLHSPQQLAEEATRKRELRLMKNREAAKECRRRKKEYVKCLESRVAVLEVQNKKLIEELETLKDICSPKTDY cd8b_human_gene T-cell surface glycoprotein CD8 beta chain (CD8B) human gene gene gene human Homo sapiens Human crem-cd8b-1 1767877 n/a 1767878 unspecified role unspecified role bait bait n/a 1767879 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 5c, 5d Q03060 decreases expression at RNA level of ENSG00000172116 anti-CD3 and anti-CD28 antibodies Stimulation of CD8+ T cells from healthy donors with anti-CD3 and anti-CD28 antibodies resulted in increased binding of CREM and decreased binding of phospho-CREB to the CD8B gene promoter. Stimulated CD8+ T cells from SLE patients showed increased binding of CREM and decreased binding of phospho-CREB to the CD8B gene promoter compared to stimulated CD8+ T cells from healthy donors.