IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ lal-2009-1 mouse-t-cell Mouse T cell t-cell Peripheral blood T-lymphocytes ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Lal G., Zhang N., van der Touw W., Ding Y., Ju W., Bottinger EP., Reid SP., Levy DE., Bromberg JS. IBD - Inflammatory bowel disease Results from experiments using anti-smads (2/3) and anti-AcH3 antibodies have not been included due to lack of specificity. CD4+CD25−Foxp3gfp− (naive) or CD4+CD25+Foxp3gfp+ (nTreg) cells were purified from Foxp3gfp reporter male mice. The naive cells were cultured with irradiated, syngenic, T cell-depleted splenocytes, and with various combinations of anti-CD3e, IL2, TGF-beta and the DNMT inhibitor 5-aza-2′-deoxycytidine (Aza). Jon.Bromberg@mountsinai.org Journal of immunology (Baltimore, Md. : 1950) 2009 Only protein-protein interactions imex curation imex curation dnm3b_mouse DNA (cytosine-5)-methyltransferase 3B Dnmt3b DNA methyltransferase MmuIIIB protein protein mouse Mus musculus Mouse MKGDSRHLNEEEGASGYEECIIVNGNFSDQSSDTKDAPSPPVLEAICTEPVCTPETRGRRSSSRLSKREVSSLLNYTQDMTGDGDRDDEVDDGNGSDILMPKLTRETKDTRTRSESPAVRTRHSNGTSSLERQRASPRITRGRQGRHHVQEYPVEFPATRSRRRRASSSASTPWSSPASVDFMEEVTPKSVSTPSVDLSQDGDQEGMDTTQVDAESRDGDSTEYQDDKEFGIGDLVWGKIKGFSWWPAMVVSWKATSKRQAMPGMRWVQWFGDGKFSEISADKLVALGLFSQHFNLATFNKLVSYRKAMYHTLEKARVRAGKTFSSSPGESLEDQLKPMLEWAHGGFKPTGIEGLKPNKKQPVVNKSKVRRSDSRNLEPRRRENKSRRRTTNDSAASESPPPKRLKTNSYGGKDRGEDEESRERMASEVTNNKGNLEDRCLSCGKKNPVSFHPLFEGGLCQSCRDRFLELFYMYDEDGYQSYCTVCCEGRELLLCSNTSCCRCFCVECLEVLVGAGTAEDAKLQEPWSCYMCLPQRCHGVLRRRKDWNMRLQDFFTTDPDLEEFEPPKLYPAIPAAKRRPIRVLSLFDGIATGYLVLKELGIKVEKYIASEVCAESIAVGTVKHEGQIKYVNDVRKITKKNIEEWGPFDLVIGGSPCNDLSNVNPARKGLYEGTGRLFFEFYHLLNYTRPKEGDNRPFFWMFENVVAMKVNDKKDISRFLACNPVMIDAIKVSAAHRARYFWGNLPGMNRPVMASKNDKLELQDCLEFSRTAKLKKVQTITTKSNSIRQGKNQLFPVVMNGKDDVLWCTELERIFGFPAHYTDVSNMGRGARQKLLGRSWSVPVIRHLFAPLKDYFACE mint Required for genome wide de novo methylation and is essential for development. DNA methylation is coordinated with methylation of histones foxp3_mouse_gene Forkhead box protein P3 Foxp3 gene gene mouse Mus musculus Mouse dnmt1_mouse DNA (cytosine-5)-methyltransferase 1 Dnmt1 Dnmt Met1 Uim MCMT DNA methyltransferase MmuI protein protein mouse Mus musculus Mouse MPARTAPARVPALASPAGSLPDHVRRRLKDLERDGLTEKECVREKLNLLHEFLQTEIKSQLCDLETKLHKEELSEEGYLAKVKSLLNKDLSLENGTHTLTQKANGCPANGSRPTWRAEMADSNRSPRSRPKPRGPRRSKSDSDTLSVETSPSSVATRRTTRQTTITAHFTKGPTKRKPKEESEEGNSAESAAEERDQDKKRRVVDTESGAAAAVEKLEEVTAGTQLGPEEPCEQEDDNRSLRRHTRELSLRRKSKEDPDREARPETHLDEDEDGKKDKRSSRPRSQPRDPAAKRRPKEAEPEQVAPETPEDRDEDEREEKRRKTTRKKLESHTVPVQSRSERKAAQSKSVIPKINSPKCPECGQHLDDPNLKYQQHPEDAVDEPQMLTSEKLSIYDSTSTWFDTYEDSPMHRFTSFSVYCSRGHLCPVDTGLIEKNVELYFSGCAKAIHDENPSMEGGINGKNLGPINQWWLSGFDGGEKVLIGFSTAFAEYILMEPSKEYEPIFGLMQEKIYISKIVVEFLQNNPDAVYEDLINKIETTVPPSTINVNRFTEDSLLRHAQFVVSQVESYDEAKDDDETPIFLSPCMRALIHLAGVSLGQRRATRRVMGATKEKDKAPTKATTTKLVYQIFDTFFSEQIEKYDKEDKENAMKRRRCGVCEVCQQPECGKCKACKDMVKFGGTGRSKQACLKRRCPNLAVKEADDDEEADDDVSEMPSPKKLHQGKKKKQNKDRISWLGQPMKIEENRTYYQKVSIDEEMLEVGDCVSVIPDDSSKPLYLARVTALWEDKNGQMMFHAHWFCAGTDTVLGATSDPLELFLVGECENMQLSYIHSKVKVIYKAPSENWAMEGGTDPETTLPGAEDGKTYFFQLWYNQEYARFESPPKTQPTEDNKHKFCLSCIRLAELRQKEMPKVLEQIEEVDGRVYCSSITKNGVVYRLGDSVYLPPEAFTFNIKVASPVKRPKKDPVNETLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIHCGKKKGKVNEADIKLRLYKFYRPENTHRSYNGSYHTDINMLYWSDEEAVVNFSDVQGRCTVEYGEDLLESIQDYSQGGPDRFYFLEAYNSKTKNFEDPPNHARSPGNKGKGKGKGKGKGKHQVSEPKEPEAAIKLPKLRTLDVFSGCGGLSEGFHQAGISETLWAIEMWDPAAQAFRLNNPGTTVFTEDCNVLLKLVMAGEVTNSLGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSYRRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEIQNGASNSEIPYNGEPLSWFQRQLRGSHYQPILRDHICKDMSPLVAARMRHIPLFPGSDWRDLPNIQVRLGDGVIAHKLQYTFHDVKNGYSSTGALRGVCSCAEGKACDPESRQFSTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDSYRFFGNILDRHRQVGNAVPPPLAKAIGLEIKLCLLSSARESASAAVKAKEEAATKD mbd2_mouse Methyl-CpG-binding domain protein 2 Mbd2 Methyl-CpG-binding protein MBD2 protein protein mouse Mus musculus Mouse MRAHPGGGRCCPEQEEGESAAGGSGAGGDSAIEQGGQGSALAPSPVSGVRREGARGGGRGRGRWKQAARGGGVCGRGRGRGRGRGRGRGRGRGRGRPQSGGSGLGGDGGGGAGGCGGGSGGGVAPRRDPVPFPSGSSGPGPRGPRATESGKRMDCPALPPGWKKEEVIRKSGLSAGKSDVYYFSPSGKKFRSKPQLARYLGNAVDLSSFDFRTGKMMPSKLQKNKQRLRNDPLNQNKGKPDLNTTLPIRQTASIFKQPVTKFTNHPSNKVKSDPQRMNEQPRQLFWEKRLQGLSASDVTEQIIKTMELPKGLQGVGPGSNDETLLSAVASALHTSSAPITGQVSAAVEKNPAVWLNTSQPLCKAFIVTDEDIRKQEERVQQVRKKLEEALMADILSRAADTEEVDIDMDSGDEA mecp2_mouse Methyl-CpG-binding protein 2 Mecp2 protein protein mouse Mus musculus Mouse MVAGMLGLREEKSEDQDLQGLRDKPLKFKKAKKDKKEDKEGKHEPLQPSAHHSAEPAEAGKAETSESSGSAPAVPEASASPKQRRSIIRDRGPMYDDPTLPEGWTRKLKQRKSGRSAGKYDVYLINPQGKAFRSKVELIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTGRPKAAASEGVQVKRVLEKSPGKLVVKMPFQASPGGKGEGGGATTSAQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVHETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESTKAPMPLLPSPPPPEPESSEDPISPPEPQDLSSSICKEEKMPRGGSLESDGCPKEPAKTQPMVATTTTVAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS sp1_mouse Transcription factor Sp1 Sp1 Specificity protein 1 protein protein mouse Mus musculus Mouse MSDQDHSMDEVTAVVKIEKDVGGNNGGSGNGGGAAFSQTRSSSTGSSSSSGGGGGQESQPSPLALLAATCSRIESPNENSNNSQGPSQSGGTGELDLTATQLSQGANGWQIISSSSGATPTSKEQSGNSTNGSNGSESSKNRTVSGGQYVVAATPNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQDGSGQIQIIPGANQQIIPNRGSGGNIIAAMPNLLQQAVPLQGLANNVLSGQTQYVTNVPVALNGNITLLPVNSVSAATLTPSSQAGTISSSGSQESSSQPVTSGTAISSASLVSSQASSSSFFTNANSYSTTTTTSNMGIMNFTSSGSSGTSSQGQTPQRVGGLQGSDSLNIQQNQTSGGSLQGSQQKEGEQSQQTQQQQILIQPQLVQGGQALQALQAAPLSGQTFTTQAISQETLQNLQLQAVQNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQTSSSNTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHQLPGLPLAIANTPGDHGTQLGLHGSGGDGIHDETAGGEGENSSDLQPQAGRRTRREACTCPYCKDSEGRASGDPGKKKQHICHIQGCGKVYGKTSHLRAHLRWHTGERPFMCNWSYCGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLSKHIKTHQNKKGGPGVALSVGTLPLDSGAGSEGTATPSALITTNMVAMEAICPEGIARLANSGINVMQVTELQSINISGNGF klf10_mouse Krueppel-like factor 10 Gdnfif Tieg Tieg1 Klf10 GDNF-inducible factor Transcription factor GIF Transforming growth factor-beta-inducible early growth response protein 1 protein protein mouse Mus musculus Mouse MLNFGASLQQASEGKMELISEKPREGMHPWDKAEQSDFEAVEALMSMSCDWKSHFKKYLENRPVTPVSDTSEDDSLLPGTPDLQTVPAFCLTPPYSPSDFEPSQGSNLTASAPSTGHFKSFSDAAKPPGATPFKEEEKNPLAAPPLPKAQATSVIRHTADAQLCNHQSCPVKAASILNYQDNSFRRRTHGNVEATRKNIPCAAVSPNRSKPEPSTVSDGDEKAGAALYDFAVPSSETVICRSQPAPSSPVQKSVLVSSPTVSTGGVPPLPVICQMVPLPANNSLVSTVVPSTPPSQPPAVCSPVLFMGTQVPEGTVVFVVPQPVVQSPRPPVVSPSGTRLSPIAPAPGFSPSAARVTPQIDSSRVRSHICSHPGCGKTYFKSSHLKAHVRTHTGEKPFSCSWKGCERRFARSDELSRHRRTHTGEKKFACPMCDRRFMRSDHLTKHARRHLSAKKLPNWQMEVSKLNDIALPPTPASAQ dnm3a_mouse DNA (cytosine-5)-methyltransferase 3A DNA methyltransferase MmuIIIA Cysteine methyltransferase DNMT3A Dnmt3a protein protein mouse Mus musculus Mouse MPSSGPGDTSSSSLEREDDRKEGEEQEENRGKEERQEPSATARKVGRPGRKRKHPPVESSDTPKDPAVTTKSQPMAQDSGPSDLLPNGDLEKRSEPQPEEGSPAAGQKGGAPAEGEGTETPPEASRAVENGCCVTKEGRGASAGEGKEQKQTNIESMKMEGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIAVMNAVEENQASGESQKVEEASPPAVQQPTDPASPTVATTPEPVGGDAGDKNATKAADDEPEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVCVEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPACHDSDESDSGKAVEVQNKQMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPRKSTTEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFIGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAIKEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV ap2a_mouse Transcription factor AP-2-alpha AP-2 transcription factor Activating enhancer-binding protein 2-alpha Activator protein 2 Tfap2a Ap2tf Tcfap2a protein protein mouse Mus musculus Mouse MLWKLTDNIKYEDCEDRHDGTSNGTARLPQLGTVGQSPYTSAPPLSHTPNADFQPPYFPPPYQPIYPQSQDPYSHVNDPYSLNPLHAQPQPQHPGWPGQRQSQESGLLHTHRGLPHQLSGLDPRRDYRRHEDLLHGPHALGSGLGDLPIHSLPHAIEDVPHVEDPGINIPDQTVIKKGPVSLSKSNSNAVSAIPINKDNLFGGVVNPNEVFCSVPGRLSLLSSTSKYKVTVAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYVCETEFPAKAVAEFLNRQHSDPNEQVARKNMLLATKQICKEFTDLLAQDRSPLGNSRPNPILEPGIQSCLTHFNLISHGFGSPAVCAAVTALQNYLTEALKAMDKMYLSNNPNSHTDNSAKSSDKEEKHRK egr1_mouse Early growth response protein 1 Egr1 Egr-1 Krox-24 Nerve growth factor-induced protein A Transcription factor Zif268 Zinc finger protein Krox-24 protein protein mouse Mus musculus Mouse MAAAKAEMQLMSPLQISDPFGSFPHSPTMDNYPKLEEMMLLSNGAPQFLGAAGTPEGSGGNSSSSTSSGGGGGGGSNSGSSAFNPQGEPSEQPYEHLTTESFSDIALNNEKAMVETSYPSQTTRLPPITYTGRFSLEPAPNSGNTLWPEPLFSLVSGLVSMTNPPTSSSSAPSPAASSSSSASQSPPLSCAVPSNDSSPIYSAAPTFPTPNTDIFPEPQSQAFPGSAGTALQYPPPAYPATKGGFQVPMIPDYLFPQQQGDLSLGTPDQKPFQGLENRTQQPSLTPLSTIKAFATQSGSQDLKALNTTYQSQLIKPSRMRKYPNRPSKTPPHERPYACPVESCDRRFSRSDELTRHIRIHTGQKPFQCRICMRNFSRSDHLTTHIRTHTGEKPFACDICGRKFARSDERKRHTKIHLRQKDKKADKSVVASPAASSLSSYPSPVATSYPSPATTSFPSPVPTSYSSPGSSTYPSPAHSGFPSPSVATTFASVPPAFPTQVSSFPSAGVSSSFSTSTGLSDMTATFSPRTIEIC dnmt3b-foxp3-1 1859481 N/A 1859482 unspecified role unspecified role bait bait N/A 1859483 unspecified role unspecified role prey prey upstream_ Foxp3_ enhancer_ CpG_ island_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 3Ai CHEBI:50131;5-aza-2′-deoxycytidine (Aza); a DNA methyltransferase inhibitor, causing DNA demethylation. DNMT1 and DNM3B bound much more to the upstream Foxp3 enhancer CpG island of naive cells rather than N-TReg cells. TGFbeta-induced Treg cells had much greater binding of DNMT1 and DNMT3b compared with N-Treg, or Aza plus TGFbeta-induced Treg cells. CHEBI:50131 decreases the binding of O88509 to ENSMUSG00000039521. dnmt1-foxp3-3 1859481 N/A 1859484 unspecified role unspecified role bait bait N/A 1859483 unspecified role unspecified role prey prey upstream_ Foxp3_ enhancer_ CpG_ island_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 3A, 5D CHEBI:50131;5-aza-2′-deoxycytidine (Aza); a DNA methyltransferase inhibitor, causing DNA demethylation. CHEBI:50131 decreases the binding of P13864 to ENSMUSG00000039521 DNMT1 and DNM3B bound much more to the upstream Foxp3 enhancer CpG island of naive cells rather than N-TReg cells. TGFbeta-induced Treg cells had much greater binding of DNMT1 and DNMT3b compared with N-Treg, or Aza plus TGFbeta-induced Treg cells. P01137 -TFG-beta1 P01137 increased the binding of P13864 to ENSMUSG00000039521 P13864 decreases expression at RNA level of ENSMUSG00000039521 P13864 increases methylation of ENSMUSG00000039521 P08505 decreases expression at RNA level of ENSMUSG00000039521 P42227 is required for the P08505 induced decrease in expression at RNA level of ENSMUSG00000039521 CHEBI:50131 increases expression at RNA level of ENSMUSG00000039521 CHEBI:50131 increases expression at protein level of ENSMUSG00000039521. P01137 and CHEBI:50131 increases expression at protein level of ENSMUSG00000039521. P01137 increases expression at protein level of ENSMUSG00000039521. P01137 and CHEBI:50131 indirectly demethylates ENSMUSG00000039521. CHEBI:50131 indirectly demethylates ENSMUSG00000039521. dnmt1-foxp3-4 1859481 N/A 1859485 unspecified role unspecified role bait bait N/A 1859483 unspecified role unspecified role prey prey upstream_ Foxp3_ enhancer_ CpG_ island_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 3B CHEBI:50131;5-aza-2′-deoxycytidine (Aza); a DNA methyltransferase inhibitor, causing DNA demethylation. P01137 -TFG-beta1 P01137 increased the binding of Q9Z2E1 to ENSMUSG00000039521 CHEBI:50131 decreases the binding of Q9Z2E1 to ENSMUSG00000039521 MBD2 and MeCP2 bound much more to the upstream Foxp3 enhancer CpG island of naive cells rather than N-TReg cells. TGFbeta-induced Treg cells had much greater binding of MBD2 and MeCP2 compared with N-Treg, or Aza plus TGFbeta-induced Treg cells. mbd2-foxp3-1 1859481 N/A 1859486 unspecified role unspecified role bait bait N/A 1859483 unspecified role unspecified role prey prey upstream_ Foxp3_ enhancer_ CpG_ island_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 3C CHEBI:50131;5-aza-2′-deoxycytidine (Aza); a DNA methyltransferase inhibitor, causing DNA demethylation. MBD2 and MeCP2 bound much more to the upstream Foxp3 enhancer CpG island of naive cells rather than N-TReg cells. TGFbeta-induced Treg cells had much greater binding of MBD2 and MeCP2 compared with N-Treg, or Aza plus TGFbeta-induced Treg cells. CHEBI:50131 decreases the binding of Q9Z2D6 to ENSMUSG00000039521 foxp3-mecp2-1 1859481 N/A 1859487 unspecified role unspecified role bait bait N/A 1859483 unspecified role unspecified role prey prey upstream_ Foxp3_ enhancer_ CpG_ island_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 4A Sp1 binding was seen using nTreg and Aza plus TGF-beta Treg cells, but not in naive CD4+CD25− T cells, activated CD4+ T cells, or TGF-beta induced Treg cells . CHEBI:50131;5-aza-2′-deoxycytidine (Aza); a DNA methyltransferase inhibitor, causing DNA demethylation. CHEBI:50131 increases the binding of O89090 to ENSMUSG00000039521 sp1-foxp3-1 1859481 N/A 1859488 unspecified role unspecified role bait bait N/A 1859483 unspecified role unspecified role prey prey upstream_ Foxp3_ enhancer_ CpG_ island_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 4D CHEBI:50131;5-aza-2′-deoxycytidine (Aza); a DNA methyltransferase inhibitor, causing DNA demethylation. CHEBI:50131 increases the binding of O89091 to ENSMUSG00000039521 O89091 Tieg1 binding was seen using nTreg and Aza plus TGF-beta Treg cells, but not in naive CD4+CD25− T cells, activated CD4+ T cells, or TGF-beta induced Treg cells . dnmt3a-foxp3-1 1859481 N/A 1859489 unspecified role unspecified role bait bait N/A 1859483 unspecified role unspecified role prey prey upstream_ Foxp3_ enhancer_ CpG_ island_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Supp 3A There was no significant difference in DNMT3a binding between nTReg and naive cells. tfap2a-foxp3-1 1859481 N/A 1859490 unspecified role unspecified role bait bait N/A 1859483 unspecified role unspecified role prey prey upstream_ Foxp3_ enhancer_ CpG_ island_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Supp 3B There was no significant difference in binding between nTReg and naive cells. tfap2a-foxp3-2 1859481 N/A 1859491 unspecified role unspecified role bait bait N/A 1859483 unspecified role unspecified role prey prey upstream_ Foxp3_ enhancer_ CpG_ island_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Supp 3C The text states that:- "were not differentially bound in these subsets" - referring to nTReg and naive cells.