MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint morishita-2008-2 human-22rv1 Homo sapiens 22rv1 Human prostate adenocarcinoma cell line Also known as CWR22Rv1 or Rv1. Cells dsplay a NE (neuroendocrine phenotype). anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Morishita D., Katayama R., Sekimizu K., Tsuruo T., Fujita N. IBD - Inflammatory bowel disease Cancer - Interactions investigated in the context of cancer In figure 2B the short isoform of PIM1 is stated to have been detected, but as no M.Wt info is visible and the antibody used detects both isoforms, no isoform information has been entered here. naoya.fujita@jfcr.or.jp Cancer Res. (0008-5472) 2008 IMEx Morishita D., Katayama R., Sekimizu K., Tsuruo T., Fujita N., Morishita D., Katayama R., Sekimizu K., Tsuruo T., Fujita N. Interactions including HA-p27Kip1 and recombinant p27Kip1 have not been included as the origin of the construct/protein cannot be traced. Only protein-protein interactions partial coverage imex curation rapid curation morishita-2008-3 in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Morishita D., Katayama R., Sekimizu K., Tsuruo T., Fujita N., Morishita D., Katayama R., Sekimizu K., Tsuruo T., Fujita N. IBD - Inflammatory bowel disease Cancer - Interactions investigated in the context of cancer Interactions including HA-p27Kip1 and recombinant p27Kip1 have not been included as the origin of the construct/protein cannot be traced. naoya.fujita@jfcr.or.jp Cancer Res. (0008-5472) 2008 imex curation Only protein-protein interactions partial coverage rapid curation morishita-2008-1 Pim kinases promote cell cycle progression by phosphorylating and down-regulating p27Kip1 at the transcriptional and posttranscriptional levels. human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Morishita D., Katayama R., Sekimizu K., Tsuruo T., Fujita N. IBD - Inflammatory bowel disease Cancer - Interactions investigated in the context of cancer In figure 2B the short isoform of PIM1 is stated to have been detected, but as no M.Wt info is visible and the antibody used detects both isoforms, no isoform information has been entered here. naoya.fujita@jfcr.or.jp Cancer Res. (0008-5472) 2008 IMEx Morishita D., Katayama R., Sekimizu K., Tsuruo T., Fujita N., Morishita D., Katayama R., Sekimizu K., Tsuruo T., Fujita N. Interactions including HA-p27Kip1 and recombinant p27Kip1 have not been included as the origin of the construct/protein cannot be traced. Only protein-protein interactions partial coverage imex curation rapid curation cdn1b_human Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1 CDKN1B KIP1 protein protein human Homo sapiens Human MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT p11309-1 Serine/threonine-protein kinase pim-1 Pim-1 PIM1 protein protein human Homo sapiens Human MLLSKINSLAHLRAAPCNDLHATKLAPGKEKEPLESQYQVGPLLGSGGFGSVYSGIRVSDNLPVAIKHVEKDRISDWGELPNGTRVPMEVVLLKKVSSGFSGVIRLLDWFERPDSFVLILERPEPVQDLFDFITERGALQEELARSFFWQVLEAVRHCHNCGVLHRDIKDENILIDLNRGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSAAVWSLGILLYDMVCGDIPFEHDEEIIRGQVFFRQRVSSECQHLIRWCLALRPSDRPTFEEIQNHPWMQDVLLPQETAEIHLHSLSPGPSK Initiates from CTG codon. foxo3_human Forkhead box protein O3 FOXO3 FKHRL1 FOXO3A Forkhead in rhabdomyosarcoma-like 1 AF6q21 protein protein protein human Homo sapiens Human MAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPESADDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSPIMASTELDEVQDDDAPLSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPSQPSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSLSHSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLSESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG E5B4E830665A9982 ly294002 LY294002 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one 2-(4-Morpholinyl)-8-phenyl-4H-1-benzopyran-4-one LY 294002 2-(morpholin-4-yl)-8-phenyl-4H-chromen-4-one small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) pim1-cdkn1b-2 985280 N/A 985283 unspecified role unspecified role bait bait N/A 985284 unspecified role unspecified role prey prey physical association physical association false false false 2B foxo3-pim1-1 985280 N/A 985285 unspecified role unspecified role bait bait N/A 985284 unspecified role unspecified role prey prey physical association physical association false false false 2Bi pim1-1 985281 N/A 985284 putative self putative self putative self putative self n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false true false Supp 1 ly294002-pim1-1 985281 N/A 985286 inhibitor inhibitor neutral component neutral component N/A 985284 putative self putative self putative self putative self n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false Supp 1i cdkn1b-pim1-1 985282 N/A 985283 unspecified role unspecified role bait bait N/A 985284 unspecified role unspecified role prey prey physical association physical association false false false 2B foxo3-pim1-2 985282 N/A 985285 unspecified role unspecified role bait bait N/A 985284 unspecified role unspecified role prey prey physical association physical association false false false 2Bi