UniProt UniProt http://www.uniprot.org fujino-2003-1 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Fujino T., Sato Y., Une M., Kanayasu-Toyoda T., Yamaguchi T., Shudo K., Inoue K., Nishimaki-Mogami T., Fujino T., Sato Y., Une M., Kanayasu-Toyoda T., Yamaguchi T., Shudo K., Inoue K., Nishimaki-Mogami T. IBD - Inflammatory bowel disease FXR ligand binding domain has been preincubated with or without different ligands (CDCA, TDCA, GDCA, CDC-sul, UDCA CA and LCA) mogami@nihs.go.jp The Journal of steroid biochemistry and molecular biology 2003 Only protein-protein interactions imex curation imex curation q96ri1-2 Bile acid receptor NR1H4 BAR FXR Farnesoid X-activated receptor Farnesol receptor HRR-1 HRR1 Nuclear receptor subfamily 1 group H member 4 RIP14 Retinoid X receptor-interacting protein 14 FXRalpha1(-) FXRalpha2 protein protein human Homo sapiens Human MGSKMNLIEHSHLPTTDEFSFSENLFGVLTEQVAGPLGQNLEVEPYSQYSNVQFPQVQPQISSSSYYSNLGFYPQQPEEWYSPGIYELRRMPAETLYQGETEVAEMPVTKKPRMGASAGRIKGDELCVVCGDRASGYHYNALTCEGCKGFFRRSITKNAVYKCKNGGNCVMDMYMRRKCQECRLRKCKEMGMLAECLLTEIQCKSKRLRKNVKQHADQTVNEDSEGRDLRQVTSTTKSCREKTELTPDQQTLLHFIMDSYNKQRMPQEITNKILKEEFSAEENFLILTEMATNHVQVLVEFTKKLPGFQTLDHEDQIALLKGSAVEAMFLRSAEIFNKKLPSGHSDLLEERIRNSGISDEYITPMFSFYKSIGELKMTQEEYALLTAIVILSPDRQYIKDREAVEKLQEPLLDVLQKLCKIHQPENPQHFACLLGRLTELRTFNHHHAEMLMSWRVNDHKFTPLLCEIWDVQ Produced by alternative splicing of isoform 3. chenodeoxycholic acid chenodeoxycholic acid 3alpha,7alpha-Dihydroxy-5beta-cholanic acid 7alpha-hydroxylithocholic acid anthropodeoxycholic acid anthropodesoxycholic acid CDCA chenic acid Chenix Chenodeoxycholic acid Chenodiol gallodesoxycholic acid 3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ncoa1_human Nuclear receptor coactivator 1 Steroid receptor coactivator 1 RIP160 Protein Hin-2 Renal carcinoma antigen NY-REN-52 NCOA1 SRC1 BHLHE74 Class E basic helix-loop-helix protein 74 protein protein human Homo sapiens Human MSGLGDSSSDPANPDSHKRKGSPCDTLASSTEKRRREQENKYLEELAELLSANISDIDSLSVKPDKCKILKKTVDQIQLMKRMEQEKSTTDDDVQKSDISSSSQGVIEKESLGPLLLEALDGFFFVVNCEGRIVFVSENVTSYLGYNQEELMNTSVYSILHVGDHAEFVKNLLPKSLVNGVPWPQEATRRNSHTFNCRMLIHPPDEPGTENQEACQRYEVMQCFTVSQPKSIQEDGEDFQSCLICIARRLPRPPAITGVESFMTKQDTTGKIISIDTSSLRAAGRTGWEDLVRKCIYAFFQPQGREPSYARQLFQEVMTRGTASSPSYRFILNDGTMLSAHTKCKLCYPQSPDMQPFIMGIHIIDREHSGLSPQDDTNSGMSIPRVNPSVNPSISPAHGVARSSTLPPSNSNMVSTRINRQQSSDLHSSSHSNSSNSQGSFGCSPGSQIVANVALNQGQASSQSSNPSLNLNNSPMEGTGISLAQFMSPRRQVTSGLATRPRMPNNSFPPNISTLSSPVGMTSSACNNNNRSYSNIPVTSLQGMNEGPNNSVGFSASSPVLRQMSSQNSPSRLNIQPAKAESKDNKEIASILNEMIQSDNSSSDGKPLDSGLLHNNDRLSDGDSKYSQTSHKLVQLLTTTAEQQLRHADIDTSCKDVLSCTGTSNSASANSSGGSCPSSHSSLTERHKILHRLLQEGSPSDITTLSVEPDKKDSASTSVSVTGQVQGNSSIKLELDASKKKESKDHQLLRYLLDKDEKDLRSTPNLSLDDVKVKVEKKEQMDPCNTNPTPMTKPTPEEIKLEAQSQFTADLDQFDQLLPTLEKAAQLPGLCETDRMDGAVTSVTIKSEILPASLQSATARPTSRLNRLPELELEAIDNQFGQPGTGDQIPWTNNTVTAINQSKSEDQCISSQLDELLCPPTTVEGRNDEKALLEQLVSFLSGKDETELAELDRALGIDKLVQGGGLDVLSERFPPQQATPPLIMEERPNLYSQPYSSPSPTANLPSPFQGMVRQKPSLGTMPVQVTPPRGAFSPGMGMQPRQTLNRPPAAPNQLRLQLQQRLQGQQQLIHQNRQAILNQFAATAPVGINMRSGMQQQITPQPPLNAQMLAQRQRELYSQQHRQRQLIQQQRAMLMRQQSFGNNLPPSSGLPVQMGNPRLPQGAPQQFPYPPNYGTNPGTPPASTSPFSQLAANPEASLANRNSMVSRGMTGNIGGQFGTGINPQMQQNVFQYPGAGMVPQGEANFAPSLSPGSSMVPMPIPPPQSSLLQQTPPASGYQSPDMKAWQQGAIGNNNVFSQAVQNQPTPAQPGVYNNMSITVSMAGGNTNVQNMNPMMAQMQMSSLQMPGMNTVCPEQINDPALRHTGLYCNQLSSTDLLKTEADGTQQVQQVQVFADVQCTVNLVGGDPYLNQPGPLGTQKPTSGPQTPQAQQKSLLQQLLTE glycochenodeoxycholic acid glycochenodeoxycholic acid chenodeoxycholylglycine Chenodeoxyglycocholate chenodeoxyglycocholic acid GCDCA glycine chenodeoxycholate Glycochenodeoxycholate GLYCOCHENODEOXYCHOLIC ACID Glycochenodeoxycholic acid [(3alpha,7alpha-dihydroxy-24-oxo-5beta-cholan-24-yl)amino]acetic acid N-(3alpha,7alpha-dihydroxy-5beta-cholan-24-oyl)glycine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) taurochenodeoxycholic acid taurochenodeoxycholic acid Chenodeoxycholoyltaurine taurine chenodeoxycholate taurochenodeoxycholate taurochenodeoxycholic acid Taurochenodeoxycholic acid 2-[(3alpha,7alpha-dihydroxy-24-oxo-5beta-cholan-24-yl)amino]ethanesulfonic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) sodium 3alpha,7alpha-dihydroxy-5beta-cholane-24-sulfonate sodium 3alpha,7alpha-dihydroxy-5beta-cholane-24-sulfonate CDC-SUL sodium 3alpha,7alpha-dihydroxy-5beta-cholane-24-sulfonate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ncoa1-nr1h4-2 1894569 N/A 1894570 unspecified role unspecified role prey prey n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1894571 stimulator stimulator unspecified role unspecified role N/A 1894572 unspecified role unspecified role bait bait undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q15788:p.[Leu690Ala;Leu693Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false c-terminus sern L-serine amide (2S)-2-amino-3-hydroxypropanamide L-serine amide MOD_RES Serine amide amidated L-serine serinamide SerN c-term range C-terminal range c-term range c-term range C-terminal range c-term range false direct interaction direct interaction false false false 1894569 1, 2, Table 1 kd without CDCA 36.2 x 10-6 molar CHEBI:16755 is required for the binding of Q15788 to Q96RI1-2 CHEBI:16755 - chenodeoxycholic acid ncoa1-nr1h4-3 1894569 N/A 1894570 unspecified role unspecified role prey prey n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1894573 stimulator stimulator unspecified role unspecified role N/A 1894572 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus sern L-serine amide (2S)-2-amino-3-hydroxypropanamide L-serine amide MOD_RES Serine amide amidated L-serine serinamide SerN c-term range C-terminal range c-term range c-term range C-terminal range c-term range false undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1894569 Table 1, fig 3 CHEBI:36274 is required for the binding of Q15788 to Q96RI1-2 CHEBI:36274 -glycochenodeoxycholic acid ncoa1-nr1h4-1 1894569 N/A 1894570 unspecified role unspecified role prey prey n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1894574 stimulator stimulator unspecified role unspecified role N/A 1894572 unspecified role unspecified role bait bait undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain false region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus sern L-serine amide (2S)-2-amino-3-hydroxypropanamide L-serine amide MOD_RES Serine amide amidated L-serine serinamide SerN c-term range C-terminal range c-term range c-term range C-terminal range c-term range false direct interaction direct interaction false false false 1894569 Table 1, fig 3 taurochenodeoxycholic acid CHEBI:16525 CHEBI:16525 is required for the binding of Q15788 to Q96RI1-2 ncoa1-nr1h4-11 1894569 N/A 1894570 unspecified role unspecified role prey prey n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false nucl_hrmn_rcpt_lig-bd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1894575 stimulator stimulator unspecified role unspecified role N/A 1894572 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminus sern L-serine amide (2S)-2-amino-3-hydroxypropanamide L-serine amide MOD_RES Serine amide amidated L-serine serinamide SerN c-term range C-terminal range c-term range c-term range C-terminal range c-term range false undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1894569 Table 1, fig 3 CHEBI:90999 CHEBI:90999 is required for the binding of Q15788 to Q96RI1-2