IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ xu-2003c-5 STAT-1 and c-Fos interaction in nitric oxide synthase-2 gene activation. in vitro In vitro emsa supershift electrophoretic mobility supershift assay emsa supershift autoradiography autoradiography Xu W., Comhair S.A., Zheng S., Chu S.C., Marks-Konczalik J., Moss J., Haque S.J., Erzurum S.C. Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease The HA-tagged human STAT1 expression construct is described in PMID:8940092. erzurus@ccf.org Am. J. Physiol. Lung Cell Mol. Physiol. (1040-0605) 2003 Only protein-protein interactions imex curation IBD - Inflammatory bowel disease xu-2003c-4 STAT-1 and c-Fos interaction in nitric oxide synthase-2 gene activation. human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xu W., Comhair S.A., Zheng S., Chu S.C., Marks-Konczalik J., Moss J., Haque S.J., Erzurum S.C. Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease Cells were stimulated with a cytokine mixture containing human interferon gamma (IFNgamma) (P01579), interleukin-1 beta (IL-1beta) (P01584) and tumor necrosis factor alpha (TNF-alpha) (P01375) prior to the binding assay. The HA-tagged human STAT1 expression construct is described in PMID:8940092. erzurus@ccf.org Am. J. Physiol. Lung Cell Mol. Physiol. (1040-0605) 2003 Only protein-protein interactions imex curation IBD - Inflammatory bowel disease xu-2003c-1 STAT-1 and c-Fos interaction in nitric oxide synthase-2 gene activation. human-bronchial_epithelial Homo sapiens bronchial_epithelium bronchial epithelium anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xu W., Comhair S.A., Zheng S., Chu S.C., Marks-Konczalik J., Moss J., Haque S.J., Erzurum S.C. Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease The HA-tagged human STAT1 expression construct is described in PMID:8940092. erzurus@ccf.org Am. J. Physiol. Lung Cell Mol. Physiol. (1040-0605) 2003 Only protein-protein interactions imex curation IBD - Inflammatory bowel disease xu-2003c-2 STAT-1 and c-Fos interaction in nitric oxide synthase-2 gene activation. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Xu W., Comhair S.A., Zheng S., Chu S.C., Marks-Konczalik J., Moss J., Haque S.J., Erzurum S.C. Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease Cells were stimulated with a cytokine mixture containing human interferon gamma (IFNgamma) (P01579), interleukin-1 beta (IL-1beta) (P01584) and tumor necrosis factor alpha (TNF-alpha) (P01375) prior to the binding assay. The HA-tagged human STAT1 expression construct is described in PMID:8940092. erzurus@ccf.org Am. J. Physiol. Lung Cell Mol. Physiol. (1040-0605) 2003 Only protein-protein interactions imex curation IBD - Inflammatory bowel disease xu-2003c-3 STAT-1 and c-Fos interaction in nitric oxide synthase-2 gene activation. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xu W., Comhair S.A., Zheng S., Chu S.C., Marks-Konczalik J., Moss J., Haque S.J., Erzurum S.C. Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease Cells were stimulated with a cytokine mixture containing human interferon gamma (IFNgamma) (P01579), interleukin-1 beta (IL-1beta) (P01584) and tumor necrosis factor alpha (TNF-alpha) (P01375) prior to the binding assay. The HA-tagged human STAT1 expression construct is described in PMID:8940092. erzurus@ccf.org Am. J. Physiol. Lung Cell Mol. Physiol. (1040-0605) 2003 Only protein-protein interactions imex curation IBD - Inflammatory bowel disease nos2_human_ap1u/gas-site_dna-probe Human NOS2 gene promoter activator protein 1 upstream and gamma-activated site DNA probe dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid human Homo sapiens Human GCCAGCTTGAGTCACACTCCAGACCACCGGGCGTTTCCAGTAAAAATC fos_human Proto-oncogene c-Fos Cellular oncogene fos G0/G1 switch regulatory protein 7 FOS G0S7 protein protein human Homo sapiens Human MMFSGFNADYEASSSRCSSASPAGDSLSYYHSPADSFSSMGSPVNAQDFCTDLAVSSANFIPTVTAISTSPDLQWLVQPALVSSVAPSQTRAPHPFGVPAPSAGAYSRAGVVKTMTGGRAQSIGRRGKVEQLSPEEEEKRRIRRERNKMAAAKCRNRRRELTDTLQAETDQLEDEKSALQTEIANLLKEKEKLEFILAAHRPACKIPDDLGFPEEMSVASLDLTGGLPEVATPESEEAFTLPLLNDPEPKPSVEPVKSISSMELKTEPFDDFLFPASSRPSGSETARSVPDMDLSGSFYAADWEPLHSGSLGMGPMATELEPLCTPVVTCTPSCTAYTSSFVFTYPEADSFPSCAAAHRKGSSSNEPSSDSLSSPTLLAL jun_human Transcription factor AP-1 JUN Activator protein 1 Proto-oncogene c-Jun V-jun avian sarcoma virus 17 oncogene homolog p39 protein protein human Homo sapiens Human MTAKMETTFYDDALNASFLPSESGPYGYSNPKILKQSMTLNLADPVGSLKPHLRAKNSDLLTSPDVGLLKLASPELERLIIQSSNGHITTTPTPTQFLCPKNVTDEQEGFAEGFVRALAELHSQNTLPSVTSAAQPVNGAGMVAPAVASVAGGSGSGGFSASLHSEPPVYANLSNFNPGALSSGGGAPSYGAAGLAFPAQPQQQQQPPHHLPQQMPVQHPRLQALKEEPQTVPEMPGETPPLSPIDMESQERIKAERKRMRNRIAASKCRKRKLERIARLEEKVKTLKAQNSELASTANMLREQVAQLKQKVMNHVNSGCQLMLTQQLQTF stat1_human Signal transducer and activator of transcription 1-alpha/beta STAT1 Transcription factor ISGF-3 components p91/p84 protein protein human Homo sapiens Human MSQWYELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAANDVSFATIRFHDLLSQLDDQYSRFSLENNFLLQHNIRKSKRNLQDNFQEDPIQMSMIIYSCLKEERKILENAQRFNQAQSGNIQSTVMLDKQKELDSKVRNVKDKVMCIEHEIKSLEDLQDEYDFKCKTLQNREHETNGVAKSDQKQEQLLLKKMYLMLDNKRKEVVHKIIELLNVTELTQNALINDELVEWKRRQQSACIGGPPNACLDQLQNWFTIVAESLQQVRQQLKKLEELEQKYTYEHDPITKNKQVLWDRTFSLFQQLIQSSFVVERQPCMPTHPQRPLVLKTGVQFTVKLRLLVKLQELNYNLKVKVLFDKDVNERNTVKGFRKFNILGTHTKVMNMEESTNGSLAAEFRHLQLKEQKNAGTRTNEGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVAEPRNLSFFLTPPCARWAQLSEVLSWQFSSVTKRGLNVDQLNMLGEKLLGPNASPDGLIPWTRFCKENINDKNFPFWLWIESILELIKKHLLPLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPDFHAVEPYTKKELSAVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDGPKGTGYIKTELISVSEVHPSRLQTTDNLLPMSPEEFDEVSRIVGSVEFDSMMNTV nos2_human_gas_dna-probe Human NOS2 gene promoter gamma-activated site DNA probe dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid human Homo sapiens Human CGGGCGTTTCCAGTAAAAATC nos2_human_ap1u-site_dna-probe Human NOS2 gene promoter activator protein 1 upstream site DNA probe dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid human Homo sapiens Human GCCAGCTTGAGTCACACTCCA fosl2_human Fos-related antigen 2 FOSL2 FRA2 protein protein human Homo sapiens Human MYQDYPGNFDTSSRGSSGSPAHAESYSSGGGGQQKFRVDMPGSGSAFIPTINAITTSQDLQWMVQPTVITSMSNPYPRSHPYSPLPGLASVPGHMALPRPGVIKTIGTTVGRRRRDEQLSPEEEEKRRIRRERNKLAAAKCRNRRRELTEKLQAETEELEEEKSGLQKEIAELQKEKEKLEFMLVAHGPVCKISPEERRSPPAPGLQPMRSGGGSVGAVVVKQEPLEEDSPSSSSAGLDKAQRSVIKPISIAGGFYGEEPLHTPIVVTSTPAVTPGTSNLVFTYPSVLEQESPASPSESCSKAHRRSSSSGDQSSDSLNSPTLLAL jund_human Transcription factor jun-D JUND protein protein human Homo sapiens Human METPFYGDEALSGLGGGASGSGGSFASPGRLFPGAPPTAAAGSMMKKDALTLSLSEQVAAALKPAAAPPPTPLRADGAPSAAPPDGLLASPDLGLLKLASPELERLIIQSNGLVTTTPTSSQFLYPKVAASEEQEFAEGFVKALEDLHKQNQLGAGAAAAAAAAAAGGPSGTATGSAPPGELAPAAAAPEAPVYANLSSYAGGAGGAGGAATVAFAAEPVPFPPPPPPGALGPPRLAALKDEPQTVPDVPSFGESPPLSPIDMDTQERIKAERKRLRNRIAASKCRKRKLERISRLEEKVKTLKSQNTELASTASLLREQVAQLKQKVLSHVNSGCQLLPQHQVPAY nos2_human_ap1d-site_dna-probe Human NOS2 gene promoter activator protein 1 downstream site DNA probe dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid human Homo sapiens Human ATTTGTGTGACTCACGCCCTC nos2-fos 1775449 n/a 1775454 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1775455 unspecified role unspecified role prey prey human-bronchial_epithelial Homo sapiens bronchial_epithelium bronchial epithelium physical association physical association false false false Fig. 5 human interferon gamma (IFNgamma) (P01579) P01579 indirectly increases expression at RNA level of ENSG00000007171 P01579 indirectly increases expression at protein level of ENSG00000007171 nos2-jun 1775449 n/a 1775454 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1775456 unspecified role unspecified role prey prey human-bronchial_epithelial Homo sapiens bronchial_epithelium bronchial epithelium physical association physical association false false false Fig. 5 human interferon gamma (IFNgamma) (P01579) nos2-stat1 1775449 n/a 1775454 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1775457 unspecified role unspecified role prey prey human-bronchial_epithelial Homo sapiens bronchial_epithelium bronchial epithelium physical association physical association false false false Fig. 5 human interferon gamma (IFNgamma) (P01579) nos2-stat1-1 1775449 n/a 1775457 unspecified role unspecified role prey prey human-bronchial_epithelial Homo sapiens bronchial_epithelium bronchial epithelium n/a 1775458 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false Fig. 5, 6a, 6b human interferon gamma (IFNgamma) (P01579) Binding was also detected using cell extracts from cells stimulated with a cytokine mixture containing human interferon gamma (IFNgamma) (P01579), interleukin-1 beta (IL-1beta) (P01584) and tumor necrosis factor alpha (TNF-alpha) (P01375). nos2-fos-1 1775449 n/a 1775458 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1775455 unspecified role unspecified role prey prey human-bronchial_epithelial Homo sapiens bronchial_epithelium bronchial epithelium physical association physical association false false false Fig. 5, 6a, 6b human interferon gamma (IFNgamma) (P01579) Binding was also detected using cell extracts from cells stimulated with a cytokine mixture containing human interferon gamma (IFNgamma) (P01579), interleukin-1 beta (IL-1beta) (P01584) and tumor necrosis factor alpha (TNF-alpha) (P01375). nos2-fos-2 1775449 n/a 1775459 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1775455 unspecified role unspecified role prey prey human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma physical association physical association false false false Fig. 7a, 7c cytokine mixture containing human interferon gamma (IFNgamma) (P01579), interleukin-1 beta (IL-1beta) (P01584) and tumor necrosis factor alpha (TNF-alpha) (P01375) P01100 increases expression at RNA level of ENSG00000007171 P01579 and P01584 and P01375 indirectly increases expression at RNA level of ENSG00000007171 P01579 and P01584 and P01375 indirectly increases expression at protein level of ENSG00000007171 nos2-fosl2 1775449 n/a 1775459 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1775460 unspecified role unspecified role prey prey human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma physical association physical association false false false Fig. 7a cytokine mixture containing human interferon gamma (IFNgamma) (P01579), interleukin-1 beta (IL-1beta) (P01584) and tumor necrosis factor alpha (TNF-alpha) (P01375) nos2-jun-1 1775449 n/a 1775459 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1775456 unspecified role unspecified role prey prey human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma physical association physical association false false false Fig. 7a cytokine mixture containing human interferon gamma (IFNgamma) (P01579), interleukin-1 beta (IL-1beta) (P01584) and tumor necrosis factor alpha (TNF-alpha) (P01375) nos2-jund 1775449 n/a 1775461 unspecified role unspecified role prey prey human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma n/a 1775459 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false Fig. 7a cytokine mixture containing human interferon gamma (IFNgamma) (P01579), interleukin-1 beta (IL-1beta) (P01584) and tumor necrosis factor alpha (TNF-alpha) (P01375) nos2-fos-1 1775449 n/a 1775455 unspecified role unspecified role prey prey human-bronchial_epithelial Homo sapiens bronchial_epithelium bronchial epithelium n/a 1775459 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false Fig. 7b nos2-jun-1 1775449 n/a 1775459 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1775457 unspecified role unspecified role prey prey human-bronchial_epithelial Homo sapiens bronchial_epithelium bronchial epithelium physical association physical association false false false Fig. 7b nos2-fos-1 1775449 n/a 1775455 unspecified role unspecified role prey prey human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma n/a 1775462 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association false false false Fig. 7c cytokine mixture containing human interferon gamma (IFNgamma) (P01579), interleukin-1 beta (IL-1beta) (P01584) and tumor necrosis factor alpha (TNF-alpha) (P01375) The interaction of c-Fos with the oligonucleotide probe containing the downstream AP-1 binding site of the human NOS2 gene promoter was much weaker compared to the interaction with the probe containing the upstream AP-1 binding site. fos-stat1-4 1775450 n/a 1775455 unspecified role unspecified role bait bait n/a 1775457 unspecified role unspecified role prey prey physical association physical association false false false Fig. 9d stat1-fos 1775450 n/a 1775457 unspecified role unspecified role bait bait n/a 1775455 unspecified role unspecified role prey prey physical association physical association false false false Fig. 9d fos-stat1-1 1775451 n/a 1775455 unspecified role unspecified role bait bait n/a 1775457 unspecified role unspecified role prey prey physical association physical association false false false Fig. 9a, 9b human interferon gamma (IFNgamma) (P01579) cytokine mixture containing human interferon gamma (IFNgamma) (P01579), interleukin-1 beta (IL-1beta) (P01584) and tumor necrosis factor alpha (TNF-alpha) (P01375) An IFN-gamma dose of 100 U/ml was necessary to detect STAT1 coimmunoprecipitation with c-Fos. fos-stat1-2 1775452 n/a 1775457 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1775455 unspecified role unspecified role bait bait naturally occurring naturally occurring endogenous endogenous physical association physical association false false false Fig. 9c fos-stat1-3 1775453 n/a 1775457 unspecified role unspecified role prey prey n/a 1775455 unspecified role unspecified role bait bait physical association physical association false false false Fig. 9c