UniProt UniProt http://www.uniprot.org takeshita-2011-1 Interaction of ataxin-3 with huntingtin-associated protein 1 through Josephin domain. mouse-neuro_2_a Mus musculus albino neuroblastoma cell line neuro 2 a (N2a) neuro_2_a Mouse Albino neuroblastoma neuro 2 a cell line fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Takeshita Y., Fujinaga R., Kokubu K., Islam M.N., Jahan M.R., Yanai A., Kakizuka A., Shinoda K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. shinoda@yamaguchi-u.ac.jp Neuroreport (0959-4965) 2011 imex curation Huntington's - Publications describing interactions involved in Huntington's disease Only protein-protein interactions takeshita-2011-2 Interaction of ataxin-3 with huntingtin-associated protein 1 through Josephin domain. mouse-neuro_2_a Mus musculus albino neuroblastoma cell line neuro 2 a (N2a) neuro_2_a Mouse Albino neuroblastoma neuro 2 a cell line anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Takeshita Y., Fujinaga R., Kokubu K., Islam M.N., Jahan M.R., Yanai A., Kakizuka A., Shinoda K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. shinoda@yamaguchi-u.ac.jp Neuroreport (0959-4965) 2011 imex curation Huntington's - Publications describing interactions involved in Huntington's disease Only protein-protein interactions hap1_human Huntingtin-associated protein 1 Neuroan 1 HAP1 HLP1 HAP2 protein protein human Homo sapiens Human MRPKRLGRCCAGSRLGPGDPAALTCAPSPSASPAPEPSAQPQARGTGQRVGSRATSGSQFLSEARTGARPASEAGAKAGARRPSAFSAIQGDVRSMPDNSDAPWTRFVFQGPFGSRATGRGTGKAAGIWKTPAAYVGRRPGVSGPERAAFIRELEEALCPNLPPPVKKITQEDVKVMLYLLEELLPPVWESVTYGMVLQRERDLNTAARIGQSLVKQNSVLMEENSKLEALLGSAKEEILYLRHQVNLRDELLQLYSDSDEEDEDEEEEEEEKEAEEEQEEEEAEEDLQCAHPCDAPKLISQEALLHQHHCPQLEALQEKLRLLEEENHQLREEASQLDTLEDEEQMLILECVEQFSEASQQMAELSEVLVLRLENYERQQQEVARLQAQVLKLQQRCRMYGAETEKLQKQLASEKEIQMQLQEESVWVGSQLQDLREKYMDCGGMLIEMQEEVKTLRQQPPVSTGSATHYPYSVPLETLPGFQETLAEELRTSLRRMISDPVYFMERNYEMPRGDTSSLRYDFRYSEDREQVRGFEAEEGLMLAADIMRGEDFTPAEEFVPQEELGAAKKVPAEEGVMEEAELVSEETEGWEEVELELDEATRMNVVTSALEASGLGPSHLDMNYVLQQLANWQDAHYRRQLRWKMLQKGECPHGALPAASRTSCRSSCR p54252-1 Ataxin-3 ATXN3 ATX3 MJD MJD1 SCA3 Machado-Joseph disease protein 1 Spinocerebellar ataxia type 3 protein MJD1a protein protein human Homo sapiens Human MESIFHEKQEGSLCAQHCLNNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNMDDSGFFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEHWFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDLPDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDGSGMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMTQTSGTNLTSEELRKRREAYFEKQQQKQQQQQQQQQQGDLSGQSSHPCERPATSSGALGSDLGKACSPFIMFATFTLYLTYELHVIFALHYSSFPL Variant in position: 349:YELHVIFALHYSSFPL->Missing (in short isoform 1, due to a stop-gain single nucleotide polymorphism, has reduced half-life due to increased proteasomal degradation, has reduced solubility and increased tendency to form aggregates, increased localization to the nucleus). atxn3-hap1 1559424 n/a 1559426 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n/a 1559427 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P54252-1:p.Gln296[24] q27 poly-glutamine extension mutation mutation range range range range false colocalization colocalization false false false 1i atxn3-hap1-3 1559424 n/a 1559426 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n/a 1559427 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection josephin sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false 2n atxn3-hap1-4 1559424 n/a 1559426 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n/a 1559427 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P54252-1:p.Gln296[76] q79 poly-glutamine extension mutation mutation range range range range false colocalization colocalization false false false 3f atxn3-hap1-2 1559425 n/a 1559427 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection josephin sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range false n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1559426 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection physical association physical association false false false 2o atxn3-hap1-5 1559425 n/a 1559427 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P54252-1:p.Gln296[76] q79 poly-glutamine extension mutation mutation range range range range false n/a 1559426 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection physical association physical association false false false 3h atxn3-hap1-6 1559425 n/a 1559426 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection n/a 1559427 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P54252-1:p.Gln296[24] q27 poly-glutamine extension mutation mutation range range range range false physical association physical association false false false 2o and 3h