IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ivleva-2005-1 LdpA: a component of the circadian clock senses redox state of the cell. syne7 Synechococcus elongatus (strain PCC 7942) Anacystis nidulans R2 pull down pull down western blot western blot Immuno blot Immuno blot Ivleva NB., Bramlett MR., Lindahl PA., Golden SS. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species strain AMC1239 overexpressing His-tagged LdpA were used fig 3, fig S1, fig S2 sgolden@tamu.edu EMBO J. (0261-4189) 2005 kaia_syne7 Circadian clock protein KaiA see0009 kaiA Synpcc7942_1218 protein protein syne7 Synechococcus elongatus (strain PCC 7942) Anacystis nidulans R2 MLSQIAICIWVESTAILQDCQRALSADRYQLQVCESGEMLLEYAQTHRDQIDCLILVAANPSFRAVVQQLCFEGVVVPAIVVGDRDSEDPDEPAKEQLYHSAELHLGIHQLEQLPYQVDAALAEFLRLAPVETMADHIMLMGANHDPELSSQQRDLAQRLQERLGYLGVYYKRDPDRFLRNLPAYESQKLHQAMQTSYREIVLSYFSPNSNLNQSIDNFVNMAFFADVPVTKVVEIHMELMDEFAKKLRVEGRSEDILLDYRLTLIDVIAHLCEMYRRSIPRET sasa_syne7 Adaptive-response sensory-kinase SasA Synechococcus adaptive sensor protein A sasA sarS Synpcc7942_2114 protein protein syne7 Synechococcus elongatus (strain PCC 7942) Anacystis nidulans R2 MGESLSPQALAQPLLLQLFVDTRPLSQHIVQRVKNILAAVEATVPISLQVINVADQPQLVEYYRLVVTPALVKIGPGSRQVLSGIDLTDQLANQLPQWLVQQEAFFADREPPEVNIPFTELGQPETPALQQADAFFQLQQQYADLSERTKFLEQVIALVAHDLRNPLTAALLAVDTIQIRSQSFSVATAKEMQGLCSLFDQARSQLREIERMIAEILEATRHSGESLRINPREVVFEPLLQQVLEQLHERWRSKQQQLITDVPGDLPTLYADPDRLRQVLVNLLDNAIKYTPPGGTITIAALHRTSQKVQISISDTGSGIPRDQLSVIFKNLVRLSRDSSQEGYGIGLSVCQRIVQAHFGRIWVASELGQGSTFHFTMPVYRYTMPC q9khi5_syne7 cikA Synpcc7942_0644 protein protein syne7 Synechococcus elongatus (strain PCC 7942) Anacystis nidulans R2 MLAPSSNCSLASQRLTPEGFAQLQSALQDFVATLPQAFYWDSRSLHTHLRTQTGDCAIAIAAGFQLLLLGRTAAEYCQPHPLSEPHHVSVQFGADSIQRYCQATNLPVEYQPALAQLGDLSLNPDLISQFSNLLIAAIAADRAPLAAQYPAVSVCQPLEQALHWQEEQDRLISQVSAQIRLSLDLSEILTTTIREIRQLLNADRAIIYQFKPQCLDAGLDQRWPLYIPSQSYITYEDRRNEALLSVIDPLVQPGLLITTEEWQRFQQGETLLIDSVGFYKERLPEQYSFYERVQVRSVCKIPILVQGRIWGLLVAHQCQQDHRWQPRERDILQHLAEHLSIAIYQAQLYGQLQDQTQTLENRVLERTQELIDALALAQAANAAKGEFLATMSHELRTPLTCVIGMSSTLLRWAFGPLTERQREYIKAIHDSGEHLLELINDILDLSQIEAGKAALQVRPFSLSRLATQTLNTLQEKARLGEIQLMLDLQLNNRVDVFRADPKRLRQILINLLSNAVKFTEPQGTVFLRVWREGDRAIFQVSDTGIGIPESEQAQLFQKFQQLDTSIRRQYGGTGLGLALTKQLVELHGGHIQIESTVGQGSTFTVWIPEQTLIEPVEPRPSIDNLPAGHILLLEEEDEAATVVCEMLTAAGFKVIWLVDGSTALDQLDLLQPIVILMAWPPPDQSCLLLLQHLREHQADPHPPLVLFLGEPPVDPLLTAQASAILSKPLDPQLLLTTLQGLCPPNLSEGDRPSS q8gli4_syne7 ldpA Synpcc7942_0624 protein protein syne7 Synechococcus elongatus (strain PCC 7942) Anacystis nidulans R2 MSASFPLEALQAGHWFKLICGASYQHLPVIRNLALTYALAGADCVDVAAEPAVVRSALAGLAAYERLTGRDRPWLMVSLNDGEDLHFRRAWFDPDRCPTDCPRPCERVCPTDAITSTGVQRDRCYGCGRCLPICPLGLIEAQAWQVDAAALLPELLDLGINAIEIHTQVGHQKEFQQLWQQLQPWLPQLQAIAISCPAHPEAIAYLWDLQELVGNTVETVIWQTDGRPMSGDIGAGTTHAAVRFAQAMLTEGPPGHVQLAGGTNAHTVAKLQELKLLAPQVSRFVAGIACGGAARTPLAELLEPLAEQQRSLEAHPEVLQAAVTTAIALVGPLKQAVGGATRSIPAFLLRTP ldpa-cika-1 in vivo pull down: cikA, SasA and kai A interacts with ldpA 205396 N/A 205397 unspecified role unspecified role prey prey N/A 205398 unspecified role unspecified role prey prey N/A 205399 unspecified role unspecified role prey prey N/A 205400 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false LdpA interacts with CikA independently of clock proteins as shown in kaiA, kaiB or kaiC mutant strains. LdpA interacts with KaiA independently of other clock proteins as shown in kaiB or kaiC mutant strains. ldpa-kaia-1 in vivo pull down: interaction between ldpA and KaiA 205396 N/A 205400 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 205397 unspecified role unspecified role prey prey physical association physical association false false false LdpA copurifies with circadian clock protein kaiA in a circadian fashion. KaiA is detected in pull-down 2 hours after the cyanbacteria are realeased in continuous light. LdpA interacts with KaiA independently of other clock proteins as shown in kaiB or kaiC mutant strains.