IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ jane-2020-2 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Jane Doe Virus - Publications including interactions involving viral proteins This entry has been curated from a pre-print and has not been peer-reviewed. If needed, the data in this entry will be amended once the manuscript has gone through peer-review. Cells were cross-linked using DSP (dithiobis (succinimidyl propionate)) followed by Anti-HA IP. zhangh92@mail.sysu.edu.cn,zhangyw57@mail.sysu.edu.cn 2020 imex curation Zhang Y, Zhang J, Chen Y, Luo B, Yuan Y, Huang F, Yang T, Yu F, Liu J, Liu B, Song Z, Chen J, Pan T, Zhang X, Li Y, Li R, Huang W, Xiao F, Zhang H Coronavirus - Interactions investigated in the context of Coronavirus Only protein-protein interactions imex curation jane-2020-3 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Jane Doe Virus - Publications including interactions involving viral proteins This entry has been curated from a pre-print and has not been peer-reviewed. If needed, the data in this entry will be amended once the manuscript has gone through peer-review. zhangh92@mail.sysu.edu.cn,zhangyw57@mail.sysu.edu.cn 2020 imex curation Zhang Y, Zhang J, Chen Y, Luo B, Yuan Y, Huang F, Yang T, Yu F, Liu J, Liu B, Song Z, Chen J, Pan T, Zhang X, Li Y, Li R, Huang W, Xiao F, Zhang H Coronavirus - Interactions investigated in the context of Coronavirus Only protein-protein interactions imex curation jane-2020-4 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Jane Doe Virus - Publications including interactions involving viral proteins This entry has been curated from a pre-print and has not been peer-reviewed. If needed, the data in this entry will be amended once the manuscript has gone through peer-review. zhangh92@mail.sysu.edu.cn,zhangyw57@mail.sysu.edu.cn 2020 imex curation Zhang Y, Zhang J, Chen Y, Luo B, Yuan Y, Huang F, Yang T, Yu F, Liu J, Liu B, Song Z, Chen J, Pan T, Zhang X, Li Y, Li R, Huang W, Xiao F, Zhang H Coronavirus - Interactions investigated in the context of Coronavirus Only protein-protein interactions imex curation hlaa_human HLA class I histocompatibility antigen, A alpha chain HLA-A HLAA Human leukocyte antigen A protein protein human Homo sapiens Human MAVMAPRTLLLLLSGALALTQTWAGSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQRMEPRAPWIEQEGPEYWDQETRNVKAQSQTDRVDLGTLRGYYNQSEAGSHTIQIMYGCDVGSDGRFLRGYRQDAYDGKDYIALNEDLRSWTAADMAAQITKRKWEAAHEAEQLRAYLDGTCVEWLRRYLENGKETLQRTDPPKTHMTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWELSSQPTIPIVGIIAGLVLLGAVITGAVVAAVMWRRKSSDRKGGSYTQAASSDSAQGSDVSLTACKV ns8_sars2 ORF8 protein Non-structural protein 8 8 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MKFLVFLGIITTVAAFHQECSLQSCTQHQPYVVDDPCPIHFYSKWYIRVGARKSAPLIELCVDEAGSKSPIQYIDIGNYTVSCLPFTINCQEPKLGSLVVRCSFYEDFLEYHDVRVVLDFI rep-hla_a-1 3714092 N/A 3714095 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level p.Pro208Ala variant variant certain certain sequence position certain certain certain sequence position certain false p.Asp98His variant variant certain certain sequence position certain certain certain sequence position certain false p.Ile121Arg variant variant certain certain sequence position certain certain certain sequence position certain false p.Gly131Trp variant variant certain certain sequence position certain certain certain sequence position certain false p.Ile166Thr variant variant certain certain sequence position certain certain certain sequence position certain false p.Gly231Ser variant variant certain certain sequence position certain certain certain sequence position certain false p.Glu277Gln variant variant certain certain sequence position certain certain certain sequence position certain false p.Leu300Pro variant variant certain certain sequence position certain certain certain sequence position certain false p.Arg169His variant variant certain certain sequence position certain certain certain sequence position certain false partial_ecd sufficient to bind sufficient binding region sufficient to bind range range range range false p.Pro217_Ile218delinsAlaVal variant variant certain certain sequence position certain certain certain sequence position certain false p.Gln86Glu variant variant certain certain sequence position certain certain certain sequence position certain false p.Asn90Lys variant variant certain certain sequence position certain certain certain sequence position certain false p.Gln94His variant variant certain certain sequence position certain certain certain sequence position certain false p.Ile119Val variant variant certain certain sequence position certain certain certain sequence position certain false p.Arg138His variant variant certain certain sequence position certain certain certain sequence position certain false p.Asp140Tyr variant variant certain certain sequence position certain certain certain sequence position certain false p.Asn151Lys variant variant certain certain sequence position certain certain certain sequence position certain false p.Glu176Val variant variant certain certain sequence position certain certain certain sequence position certain false p.Asp185Glu variant variant certain certain sequence position certain certain certain sequence position certain false N/A 3714096 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2F Experiment was carried out in the presence of Bafilomycin A1 (Baf A1, an autophagy inhibitor). HLA-A201 sequence rep-hla_a-2 3714093 N/A 3714095 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed p.Pro208Ala variant variant certain certain sequence position certain certain certain sequence position certain false p.Asp98His variant variant certain certain sequence position certain certain certain sequence position certain false p.Ile121Arg variant variant certain certain sequence position certain certain certain sequence position certain false p.Gly131Trp variant variant certain certain sequence position certain certain certain sequence position certain false p.Ile166Thr variant variant certain certain sequence position certain certain certain sequence position certain false p.Gly231Ser variant variant certain certain sequence position certain certain certain sequence position certain false p.Glu277Gln variant variant certain certain sequence position certain certain certain sequence position certain false p.Leu300Pro variant variant certain certain sequence position certain certain certain sequence position certain false p.Arg169His variant variant certain certain sequence position certain certain certain sequence position certain false partial_ecd sufficient to bind sufficient binding region sufficient to bind range range range range false p.Pro217_Ile218delinsAlaVal variant variant certain certain sequence position certain certain certain sequence position certain false p.Gln86Glu variant variant certain certain sequence position certain certain certain sequence position certain false p.Asn90Lys variant variant certain certain sequence position certain certain certain sequence position certain false p.Gln94His variant variant certain certain sequence position certain certain certain sequence position certain false p.Ile119Val variant variant certain certain sequence position certain certain certain sequence position certain false p.Arg138His variant variant certain certain sequence position certain certain certain sequence position certain false p.Asp140Tyr variant variant certain certain sequence position certain certain certain sequence position certain false p.Asn151Lys variant variant certain certain sequence position certain certain certain sequence position certain false p.Glu176Val variant variant certain certain sequence position certain certain certain sequence position certain false p.Asp185Glu variant variant certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3714096 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2G Experiment was carried out in the presence of Bafilomycin A1 (Baf A1, an autophagy inhibitor). HLA-A201 sequence rep-hla_a-3 3714094 N/A 3714095 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level p.Pro208Ala variant variant certain certain sequence position certain certain certain sequence position certain false p.Asp98His variant variant certain certain sequence position certain certain certain sequence position certain false p.Ile121Arg variant variant certain certain sequence position certain certain certain sequence position certain false p.Gly131Trp variant variant certain certain sequence position certain certain certain sequence position certain false p.Ile166Thr variant variant certain certain sequence position certain certain certain sequence position certain false p.Gly231Ser variant variant certain certain sequence position certain certain certain sequence position certain false p.Glu277Gln variant variant certain certain sequence position certain certain certain sequence position certain false p.Leu300Pro variant variant certain certain sequence position certain certain certain sequence position certain false p.Arg169His variant variant certain certain sequence position certain certain certain sequence position certain false partial_ecd sufficient to bind sufficient binding region sufficient to bind range range range range false p.Pro217_Ile218delinsAlaVal variant variant certain certain sequence position certain certain certain sequence position certain false p.Gln86Glu variant variant certain certain sequence position certain certain certain sequence position certain false p.Asn90Lys variant variant certain certain sequence position certain certain certain sequence position certain false p.Gln94His variant variant certain certain sequence position certain certain certain sequence position certain false p.Ile119Val variant variant certain certain sequence position certain certain certain sequence position certain false p.Arg138His variant variant certain certain sequence position certain certain certain sequence position certain false p.Asp140Tyr variant variant certain certain sequence position certain certain certain sequence position certain false p.Asn151Lys variant variant certain certain sequence position certain certain certain sequence position certain false p.Glu176Val variant variant certain certain sequence position certain certain certain sequence position certain false p.Asp185Glu variant variant certain certain sequence position certain certain certain sequence position certain false N/A 3714096 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 2E HLA-A201 sequence