IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ yuan-2020-3 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yuan S., Chu H., Huang J., Zhao X., Ye ZW., Lai PM., Wen L., Cai JP., Mo Y., Cao J., Liang R., Poon VK., Sze KH., Zhou J., To KK., Chen Z., Chen H., Jin DY., Chan JF., Yuen KY. Coronavirus - Interactions investigated in the context of Coronavirus The authors have stated that HA/HIS-AP2M1 is of human origin. kyyuen@hku.hk, jfwchan@hku.hk Science advances 2020 Only protein-protein interactions imex curation imex curation Virus - Publications including interactions involving viral proteins yuan-2020-2 in vitro In vitro solid phase assay solid phase assay tag visualisation tag visualisation Yuan S., Chu H., Huang J., Zhao X., Ye ZW., Lai PM., Wen L., Cai JP., Mo Y., Cao J., Liang R., Poon VK., Sze KH., Zhou J., To KK., Chen Z., Chen H., Jin DY., Chan JF., Yuen KY. Coronavirus - Interactions investigated in the context of Coronavirus The authors have stated that HA/HIS-AP2M1 is of human origin. kyyuen@hku.hk, jfwchan@hku.hk Science advances 2020 Only protein-protein interactions imex curation imex curation Virus - Publications including interactions involving viral proteins yuan-2020-1 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel sequence tag sequence tag identification sequence tag Yuan S., Chu H., Huang J., Zhao X., Ye ZW., Lai PM., Wen L., Cai JP., Mo Y., Cao J., Liang R., Poon VK., Sze KH., Zhou J., To KK., Chen Z., Chen H., Jin DY., Chan JF., Yuen KY. Coronavirus - Interactions investigated in the context of Coronavirus A fraction from H1N1 virus-infected MDCK cells was incubated with azido-ACA, and further incubated with DyLight 488-Phosphine to allow linkage of fluorescent dye with the azido group. The authors have stated that HA/HIS-AP2M1 is of human origin. kyyuen@hku.hk, jfwchan@hku.hk Science advances 2020 Only protein-protein interactions imex curation imex curation Virus - Publications including interactions involving viral proteins ncap_i34a1 Nucleoprotein NP Nucleocapsid protein protein protein i34a1 Influenza A virus (strain A/Puerto Rico/8/1934 H1N1) MASQGTKRSYEQMETDGERQNATEIRASVGKMIGGIGRFYIQMCTELKLSDYEGRLIQNSLTIERMVLSAFDERRNKYLEEHPSAGKDPKKTGGPIYRRVNGKWMRELILYDKEEIRRIWRQANNGDDATAGLTHMMIWHSNLNDATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTMVMELVRMIKRGINDRNFWRGENGRKTRIAYERMCNILKGKFQTAAQKAMMDQVRESRNPGNAEFEDLTFLARSALILRGSVAHKSCLPACVYGPAVASGYDFEREGYSLVGIDPFRLLQNSQVYSLIRPNENPAHKSQLVWMACHSAAFEDLRVLSFIKGTKVLPRGKLSTRGVQIASNENMETMESSTLELRSRYWAIRTRSGGNTNQQRASAGQISIQPTFSVQRNLPFDRTTIMAAFNGNTEGRTSDMRTEIIRMMESARPEDVSFQGRGVFELSDEKAASPIVPSFDMSNEGSYFFGDNAEEYDN ap2m1_human AP-2 complex subunit mu Adaptor protein complex AP-2 subunit mu AP-2 mu chain AP2M1 CLAPM1 KIAA0109 Adaptin-mu2 Plasma membrane adaptor AP-2 50 kDa protein HA2 50 kDa subunit Clathrin coat assembly protein AP50 Clathrin coat-associated protein AP50 Clathrin assembly protein complex 2 mu medium chain Adaptor-related protein complex 2 subunit mu protein protein human Homo sapiens Human MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC q32ze1-pro_0000435838 Serine protease NS3 protein protein zikv Zika virus (strain Mr 766) ZIKV SGALWDVPAPKEVKKGETTDGVYRVMTRRLLGSTQVGVGVMQEGVFHTMWHVTKGAALRSGEGRLDPYWGDVKQDLVSYCGPWKLDAAWDGLSEVQLLAVPPGERARNIQTLPGIFKTKDGDIGAVALDYPAGTSGSPILDKCGRVIGLYGNGVVIKNGSYVSAITQGKREEETPVECFEPSMLKKKQLTVLDLHPGAGKTRRVLPEIVREAIKKRLRTVILAPTRVVAAEMEEALRGLPVRYMTTAVNVTHSGTEIVDLMCHATFTSRLLQPIRVPNYNLNIMDEAHFTDPSSIAARGYISTRVEMGEAAAIFMTATPPGTRDAFPDSNSPIMDTEVEVPERAWSSGFDWVTDHSGKTVWFVPSVRNGNEIAACLTKAGKRVIQLSRKTFETEFQKTKNQEWDFVITTDISEMGANFKADRVIDSRRCLKPVILDGERVILAGPMPVTHASAAQRRGRIGRNPNKPGDEYMYGGGCAETDEGHAHWLEARMLLDNIYLQDGLIASLYRPEADKVAAIEGEFKLRTEQRKTFVELMKRGDLPVWLAYQVASAGITYTDRRWCFDGTTNNTIMEDSVPAEVWTKYGEKRVLKPRWMDARVCSDHAALKSFKEFAAGKR 1499 2115 ncap_mers1 Nucleoprotein N Nucleocapsid protein 6 protein protein cvemc Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (Betacoronavirus England 1) Betacoronavirus England 1 Human coronavirus EMC MASPAAPRAVSFADNNDITNTNLSRGRGRNPKPRAAPNNTVSWYTGLTQHGKVPLTFPPGQGVPLNANSTPAQNAGYWRRQDRKINTGNGIKQLAPRWYFYYTGTGPEAALPFRAVKDGIVWVHEDGATDAPSTFGTRNPNNDSAIVTQFAPGTKLPKNFHIEGTGGNSQSSSRASSVSRNSSRSSSQGSRSGNSTRGTSPGPSGIGAVGGDLLYLDLLNRLQALESGKVKQSQPKVITKKDAAAAKNKMRHKRTSTKSFNMVQAFGLRGPGDLQGNFGDLQLNKLGTEDPRWPQIAELAPTASAFMGMSQFKLTHQNNDDHGNPVYFLRYSGAIKLDPKNPNYNKWLELLEQNIDAYKTFPKKEKKQKAPKEESTDQMSEPPKEHRVQGTQRTRTRPSVQPGPMIDVNTD b9vuu3-pro_0000449080 Protein 2C protein protein EV71 Human enterovirus 71 EV-71 SASWLKKFNDMANAAKGLEWVSNKISKFIDWLKEKIVPAAKEKVEFLNNLKQLPLLENQISNLEQSAASQEDLEVMFGNVSYLAHFCRKFQPLYATEAKRVYALEKRMNNYMQFKSKHRIEPVCLIIRGSPGTGKSLATGIIARAIADKYHSSVYSLPPDPDHFDGYKQQVVTVMDDLCQNPDGKDMSLFCQMVSTVDFIPPMASLEEKGVSFTSKFVIASTNATNIIVPTVSDSDAIRRRFYMDCDIEVTDSYKTDLGRLDAGRAAKLCSENNTANFKRCSPLVCGKAIQLRDRKSKVRYSVDTVVSELIREYSNRSAIGNTIEALFQ 1112 1440 yxxø DYQRLN peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) DYQRLN n-(p-amylcinnamoyl)anthranilic acid N-(p-amylcinnamoyl)anthranilic acid 4-amylcinnamoylanthranilic acid ACA p-amylcinnamoylanthranilic acid 2-{[(2E)-3-(4-pentylphenyl)prop-2-enoyl]amino}benzoic acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) a0a455r0b8_canlf TMPRSS2 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MPLNSGSPPGVGPYYENHGYQPESLYPPQPATAPRAYPVYPAPYYPPAVPQYTPRVLTHTSTPAIHTQPKSPLGTGCTAKTKKALCITVVLGTILAGAAVAAVLLWKFMEDKCSVSGIECGSSGTCISPSQWCDGVLHCPSGEDENRCVRLYGPNFILQVYSSQRKSWHPVCQDNWSDSYGRAACQDMGYRNSFYSSQGIADDSGATSFMKLNISAGHMDLYKKLYHSDVCSSKTVVSLRCIECGVSAKGSRQSRIVGGTSASLGDWPWQVSLHVQGTHVCGGSIISPEWIVTAAHCVEEPLNNPRYWTAFAGILRQSFMFYGHGHRVGKVISHPNYDSKTKNNDIALMKLQTPLTFNDRVKPVCLPNPGMMLEPEQSCWISGWGATYEKGKTSDELNAVMVPLIEPWRCNSKYVYNNLVTPAMICAGFLRGGVDSCQGDSGGPLVTLKSRVWWLIGDTSWGSGCAKANRPGVYGNVTVFTDWIYRQMRANS f1ppt2_canlf KIF21A protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MFFITQHVTSCMWIITTELLSRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIIEEEQGIISRAVKHLFKSIEEKKHTAIKNGHPPPDFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTESEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHLCQTRMCPQTDAENATDNKVISESSQLNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESINDMFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSDQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRATARSPYFSGSSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKRLQKLEESNREERSVASKEDNTDTDQEKKEEKGITERENNELEVEESQEVSDHEEEEEEEEEEEDDIEGGESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVRSEYEKKLQAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDRVAGKVTRKLSSSDTPVQDMGSTAAAVEADVSRAGAQQKMRIPVARVQALPTPTTNGTRKKYQRKGLTGRVFTSKTARMKWQLLERRVTDIIMQKMTISNMEADMNRLLKQREELTKRREKLSKRREKIVKESEEGDKNVININEEMESLTANIDYINDSISDCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDSVPLENVEDSTDEEAPLNSPGSEGSTLSSDLMKLCGEVKPKSKARRRTTTQMELLYADSSELASDTSTGDASLPGPLTPVAEGQEIGMNTETSGTSAREKELPPPSGFPSKIGSISRQSSLSEKKMPEPSPITRRKAYEKAEKSKAKEQKHSDSGTSEASLSPPSSPPSRPRNELNVFNRLTVSQGNTSVQQDKSDESDSSLSEVHSRSSRRGIINPFPASKGIRTSPLQCIHIAEGHTKAVLCVDSTDDLLFTGSKDRTCKVWNLVTGQEIMSLGGHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRDSAKCIRTLTSSGQVTLGDACSASTSRTVAIPSGENQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQDLIVTGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALTIQGDNLFSGSRDNGIKKWDLAQKDLLQQVPNAHKDWVCALGVVPGHPVLLSGCRGGILKLWNMDTFVPVGEMKGHESPINAICVNSTHIFTAADDRTVRIWKARNLQDGQISDIGDLGEDIASN e2quu5_canlf 60 kDa chaperonin 60 kDa heat shock protein, mitochondrial Chaperonin 60 Heat shock protein 60 HSPD1 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MLRLPAVLRHVRPVSRALAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSVQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLTLNLEDVQPHDLGKVGEVIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDITTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSITPANEDQRIGIEIIKRTLKIPAMTIAKNAGVEGSLIVEKIMQSSSEVGYDAMLGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEVVVTEIPKEEKDPGMGGMGGMGGGMGGGMF f1pk90_canlf RNA helicase DDX46 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MGRESRHYRKRSASRGRSGSRSRSRSPSDKRSKRGDDRRSRSRDRDRRRERSRSRDKRRSRSRDRKRLRRSRSRERDRSRERRRSRSRDRRRSRSRSRGRRSRSSSPGNKSKKAENRSRSKEKTDSGESSKEKKKDKDDKEDEKEKDAGNFDQNKLEEEMRKRKERVEKWREEQRKKAMENIGELKKEIEEMKQGKKWSLEDDDDDEDDPAEAEKEGNEMEGEELDPLDAYMEEVKEEVKKFNMRSVKGGGGNEKKSGPTVTKVVTVVTTKKAVVDSDKKKGELMENDQDAMEYSSEEEEVDLQTALTGYQTKQRKLLEPVDHGKIEYEPFRKNFYVEVPELAKMSQEEVNVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSLKKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHIMDQRSLEEGEGPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNVRPDRQTVMFSATFPRAMEALARRILSKPIEVQVGGRSVVCSDVEQQVIVIEEEKKFLKLLELLGHYQESGSVIIFVDKQEHADGLLKDLMRASYPCMSLHGGIDQYDRDSIINDFKNGTCKLLVATSVAARGLDVKHLILVVNYSCPNHYEDYVHRAGRTGRAGNKGYAYTFITEDQARYAGDIIKALELSGTAVPPDLEKLWSDFKDQQKAEGKIIKKSSGFSGKGFKFDETEQALANERKKLQKAALGLQDSDDEDAAVDIDEQIESMFNSKKRVKDMAAPGTSSVPAPTAGNAEKLEIAKRLALRINAQKNLGIESQVDVMQQATNAILRGGTILAPTVSAKTIAEQLAEKINAKLNYVPLEKQEEERQDGGQNESFKRYEEELEINDFPQTARWKVTSKEALQRISEYSEAAITIRGTYFPPGKEPKEGERKIYLAIESANELAVQKAKAEITRLIKEELIRLQNSYQPTNKGRYKVL e2rcj9_canlf CNTN1 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MKMWLLVSHLVIISFTSCLSEFTWYRRYGHGVSEEDKGFGPIFEEQPINTIYPEESPEGKVSLNCRARASPFPVYKWRMNNGDIDLTSDRYSMVGGNLVINNPDKQKDAGIYYCLASNNYGMVRSTEATLSFGYLDPFPPEERPEVRVKEGKGMVLLCDPPYHFPDDLSYRWLLNEFPVFITMDKRRFVSQTNGNLYIANVEASDKGNYSCFVSSPSITKSVFSKFIPLIPLPERTTKPYPADIVVQFKDIYALMGQNVTLECFALGNPVPDIRWRKVLEPMPSTAEISTSGAVLKIFNIQLEDEGTYECEAENNRGKDKHQARIYVQAFPEWVEHINDTEVDIGSDLYWPCVATGKPIPTIRWLKNGYAYHKGELRLYDVTFENAGMYQCIAENTHGTIYANAELKILALAPTFEMNPMKKKILAAKGGRVIIECKPKAAPKPKFSWSKGTEWLVNSSRILIWEDGSLEINNITRNDGGIYTCFAENNRGRANNTGTLVITDPTRIILAPINADITVGENATMQCAASFDPALDLTFIWSFNGYVIDFNKENIHYQRNFMLDSNGELLIRNAQLKHAGRYTCTAQTIVDNSSASADLVVRGPPGPPGGLRIEDIRATSVALTWSRGSDNHSPISKYTIQTKTILSDDWKDAKTDPPIIEGNMEAARAVDLIPWMEYEFRVVATNTLGIGEPSIPSNKIKTDGAAPNVAPSDVGGGGGSNRELTITWVPLSREYHYGNNFGYIVAFKPFDGEEWKKVTVTNPDTGRYVHKDETMRPSTAFQVKVKAFNNKGDGPYSLTAVINSAQDAPSEAPTEVGVKVLSSSEISVHWEHVVEKIVESYQIRYWAAHDKEAAAHRVQVTSQEYSARLENLLPDTQYFVEVRACNSAGCGPPSDMIETFTKKAPPSQPPRIISSIRSGSRYIITWDHVVALSNESTVTGYKVLYRPDGQHDGKLYSTHKHSIEVPIPRDGEYVVEVRAHSDGGDGVVSQVKISGVSTLSPTLLGLLLPALGIVVYLAI calr_canlf Calreticulin CRP55 Calregulin Endoplasmic reticulum resident protein 60 HACBP CALR protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris EPAIYFKEQFLDGXGFTDXRIKEK e2r8d3_canlf Vasopressin V1a receptor AVPR1A protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MRIPGGPGAPSAGNSSRWWPASGRGANASGDAGALADGGGPPRDARNEELAKLEIAVLAVTFVVAVLGNSSVLVALHRTPRKTSRMHLFIRHLSLADLAVAFFQVLPQMCWDITYRFRGPDGLCRVVKHLQVFGMFVSPYMLVVMTADRYIAVCHPLKTLQQPTRRSRLMIAAAWVLSLVLSTPQYLVFSMVEVNNVTKANDCWATFIQPWGPRAYVTWMTAGIFVAPVVLLATCYGCICSHIWRSVRGRTALRAGAAAGAVPRGVLRAPGASSVKTISRAKMRTVKMTFVIVTVYIVCWAPFFILQMWSVWDDGFVWIESENPAITITALLASLNSCCNPWIYMFFSGHLLQDCVQSFPCCQNMKQTFNKVDSDSVSRRQTSYTNNRSPTNSMGTWKDSPKTSKSVKFIPVST a0a5f4bq86_canlf AP-2 complex subunit mu AP2M1 protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC ap2m1-np-1 3930814 N/A 3930817 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3930818 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Figure 7A ACA -CHEBI:139349 . ap2m1-np-2 3930814 N/A 3930819 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3930818 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Figure 7B ACA -CHEBI:139349 . ap2m1-n-1 3930814 N/A 3930820 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3930818 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Figure 7C ACA -CHEBI:139349 . ap2m1-n-2 3930814 N/A 3930821 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3930818 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Supp Figure 7D ACA -CHEBI:139349 . ap2m1-yxx-1 3930815 N/A 3930822 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3930818 unspecified role unspecified role bait bait cell lysate cell lysate Q96CW1:p.Asp176Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 5B, number 1 ap2m1-yxx-2 3930815 N/A 3930822 competitor competitor competitor prey prey chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3930822 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3930818 unspecified role unspecified role bait bait cell lysate cell lysate region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 5B, number 2 ap2m1-yxx-3 3930815 N/A 3930823 inhibitor inhibitor prey prey chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3930822 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3930818 unspecified role unspecified role bait bait cell lysate cell lysate region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q96CW1:p.Asp176Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 5B, number 3 ap2m1-avpr1a-1 3930816 N/A 3930824 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line N/A 3930825 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line N/A 3930826 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line N/A 3930827 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line N/A 3930828 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line N/A 3930829 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line N/A 3930830 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line N/A 3930831 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level canlf-mdck Canis familiaris (dog) kidney epithelial cells Canis lupus familiaris Dog Canis familiaris mdck Canine Cocker Spaniel kidney epithelial cell line N/A 3930823 unspecified role unspecified role neutral component neutral component azido group tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false DyLight 488 fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) 3930881 3930882 association association false false false 4D Gel plug of the target band was collected for LC-MS/MS, which identified eight candidate proteins that were physically associated with azido-ACA.