IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ song-2019-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Song Z., Yang Y., Wang L., Wang K., Ran L., Xie Y., Huang L., Yang Z., Yuan P., Yu Q. Virus - Publications including interactions involving viral proteins UniprotAc for RNA polymerase II transcription subunit XP_005653991.1 94 Centromere-related proteins E XP_013844806.1 could not be retrieved as they are obsolete entries. Not curated. szh7678@126.com Research in veterinary science 2019 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus song-2019-3 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Song Z., Yang Y., Wang L., Wang K., Ran L., Xie Y., Huang L., Yang Z., Yuan P., Yu Q. Virus - Publications including interactions involving viral proteins szh7678@126.com Research in veterinary science 2019 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus song-2019-4 pig-iec Porcine intestinal epithelial crypt cells intestine-epithelial_crypt intestinal epithelial crypt cells (iec) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Song Z., Yang Y., Wang L., Wang K., Ran L., Xie Y., Huang L., Yang Z., Yuan P., Yu Q. Virus - Publications including interactions involving viral proteins pIECs infected with TGEV szh7678@126.com Research in veterinary science 2019 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus song-2019-5 pig-iec Porcine intestinal epithelial crypt cells intestine-epithelial_crypt intestinal epithelial crypt cells (iec) confocal microscopy confocal microscopy immunostaining immunostaining Song Z., Yang Y., Wang L., Wang K., Ran L., Xie Y., Huang L., Yang Z., Yuan P., Yu Q. Virus - Publications including interactions involving viral proteins pIECs infected with TGEV szh7678@126.com Research in veterinary science 2019 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus vme1_cvppu Membrane protein M E1 glycoprotein Matrix glycoprotein Membrane glycoprotein 5 protein protein TGEV Porcine transmissible gastroenteritis coronavirus (strain Purdue) MKILLILACVIACACGERYCAMKSDTDLSCRNSTASDCESCFNGGDLIWHLANWNFSWSIILIVFITVLQYGRPQFSWFVYGIKMLIMWLLWPVVLALTIFNAYSEYQVSRYVMFGFSIAGAIVTFVLWIMYFVRSIQLYRRTKSWWSFNPETKAILCVSALGRSYVLPLEGVPTGVTLTLLSGNLYAEGFKIAGGMNIDNLPKYVMVALPSRTIVYTLVGKKLKASSATGWAYYVKSKAGDYSTEARTDNLSEQEKLLHMV a0a4x1trk4_pig AIR carboxylase Multifunctional protein ADE2 Phosphoribosylaminoimidazole carboxylase Phosphoribosylaminoimidazole-succinocarboxamide synthase SAICAR synthetase protein protein pig Sus scrofa (Pig) Pig MLSPGRHYLSPPSCAVAAAAPSPLRTMATAEVLNIGKKLYEGKTKEVYELLDSPGKVLLQSKDQITAGNAARKNHLEGKAAISNTITSCVFQLLQEAGIKTAFTRKCGETAFIAPKCEMIPIEWVCRRIATGSFLKRNPGVKEGYKFYPPKVEMFFKDDANNDPQWSEEQLIAANFCFAGLVIGQTEVDVMSHATQAIFEILEKSWLPQNCTLVDMKIEFGVDVTTKEIVLADVIDNDSWRLWPSGDRSQQKDKQSYRDLKEVTPEGLQMVKKNFEWVAERVELLLKTESQCRVVVLMGSTSDLSHCEKIKKACGNFGIPCELRVTSAHKGPDETLRIKAEYEGDGIPTVFVAVAGRSNGLGPVMSGNTAFPVISCPPLSPDWGAQDVWSSLRLPSGLGCSTILSPEGSAQFAAQIFGLNNHLVWAKLRANVLNTWISLKQADKKIREGNL a0a4x1v3c3_pig Small nuclear ribonucleoprotein Sm D3 SNRPD3 snRNP core protein D3 protein protein pig Sus scrofa (Pig) Pig MSIGVPIKVLHEAEGHIVTCETNTGEVYRGKLIEAEDNMNCQMSNITVTYRDGRVAQLEQVYIRGSKIRFLILPDMLKNAPMLKSMKNKNQGSGAGRGKAAILKAQVAARGRGRGMGRGNIFQKRR f1s215_pig Regulator of G-protein signaling 4 RGS4 protein protein pig Sus scrofa (Pig) Pig MCKGLAGLPASCLRSAKDMKHRLGFLLQKSDSCEHSSSHSKKDKVVICQRVSQEEVKKWAESLENLISHECGLAAFKAFLKSEYSEENIDFWISCEEYKKIKSPSKLSPRAKKIYNEFISVQATKEVNLDSCTREETSRNMLEPTITCFDEAQKKIFNLMEKDSYRRFLKSRFYLDLANLSSCGSEKQKGAKSSTDCPSLVPQCA f1rnn5_pig P2X purinoceptor P2RX7 protein protein pig Sus scrofa (Pig) Pig MPAWCSWNDVFQYETNKVTRIQSMNYGTIKWFFHVLVFSYVSFALVSDKRYQRKESVISSVHTKVKGIAEVTQEIVENGLKKVVPSVLDTADYTFPLQGNSFFVMTNFLKTEGQEQGLCPEYPTRRTLCSSNQGCKKGWMDPQSKGIQTGRCVEYKGKQKTCEVSAWCPIEAVEEAPQPALLRSAENFTVLIKNNIDFPGHNYTTRNILPGLNITCTFHKTQNPLCPIFRLGDIFRETGDNFSDVAIQGGIMGIEIYWDCNLDRWFHHCRPKYSFRRLDDKTAKESSYPGYNFRYAKYYKENNVEKRTLIKVFGIRFDILVFGTGGKFDIIQLIVYIGSTLSYFGLATVFIDFLINTYSNNCCRSHVYPCCKCCEPCAVNEYYYRKKCESIMEPKPTLKYVSFVDEPHIRMVDQRLLGRSLQDVKGEEVPRPPMDFTDLSRLPLSLHDPPFIPGQPEAIQLLSEEAAPKSSDCPDWCQCGNCLPSQLPDSHKCLEELCCRKKLGACITTSELFQKLVLSRGVLQFLLHYQEPLLVLDADSANSQLRHCAYRCYTIWRFGSQDLADFAILPSCCRWKIRREFPKSEGQYSGFQSPY a6m930_pig RNA helicase EIF4A2 protein protein pig Sus scrofa (Pig) Pig MSGGSADYNREHGGPEGMDPDGVIESNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKEELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTVSALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI m-ade2-1 3710193 N/A 3710197 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 3710198 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false Fig. 1 and Table 2 m-a0a4x1trk4_pig-1 3710193 N/A 3710197 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 3710199 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false Fig. 1 and Table 2 m-snrpd3-1 3710193 N/A 3710197 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 3710200 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false Fig. 1 and Table 2 m-rgs4-1 3710193 N/A 3710197 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 3710201 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false Fig. 1 and Table 2 m-p2rx7-1 3710193 N/A 3710197 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 3710202 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false Fig. 1 and Table 2 m-eif4a2-1 3710194 N/A 3710197 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3710202 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Fig. 3A-B m-eif4a2-2 3710195 N/A 3710197 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level infection infection N/A 3710202 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig. 3C eif4a2-m-1 3710196 N/A 3710197 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level infection infection N/A 3710202 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization false false false Fig. 4 The green fluorescence of M protein and the red fluorescence of EIF4A2 were observed to be partially overlapping in the cytoplasm