DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ chang-2006-5 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column experimental particp experimental participant identification experimental particp Chang CK., Sue SC., Yu TH., Hsieh CM., Tsai CK., Chiang YC., Lee SJ., Hsiao HH., Wu WJ., Chang WL., Lin CH., Huang TH. Coronavirus - Interactions investigated in the context of Coronavirus bmthh@gate.sinica.edu.tw Journal of biomedical science 2006 imex curation Only protein-protein interactions imex curation chang-2006-7 in vitro In vitro crosslink cross-linking study crosslink experimental particp experimental participant identification experimental particp Chang CK., Sue SC., Yu TH., Hsieh CM., Tsai CK., Chiang YC., Lee SJ., Hsiao HH., Wu WJ., Chang WL., Lin CH., Huang TH. Coronavirus - Interactions investigated in the context of Coronavirus bmthh@gate.sinica.edu.tw Journal of biomedical science 2006 imex curation Only protein-protein interactions imex curation chang-2006-8 in vitro In vitro cosedimentation cosedimentation experimental particp experimental participant identification experimental particp Chang CK., Sue SC., Yu TH., Hsieh CM., Tsai CK., Chiang YC., Lee SJ., Hsiao HH., Wu WJ., Chang WL., Lin CH., Huang TH. Coronavirus - Interactions investigated in the context of Coronavirus bmthh@gate.sinica.edu.tw Journal of biomedical science 2006 imex curation Only protein-protein interactions imex curation ncap_sars Nucleoprotein N 9a Nucleocapsid protein protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MSDNGPQSNQRSAPRITFGGPTDSTDNNQNGGRNGARPKQRRPQGLPNNTASWFTALTQHGKEELRFPRGQGVPINTNSGPDDQIGYYRRATRRVRGGDGKMKELSPRWYFYYLGTGPEASLPYGANKEGIVWVATEGALNTPKDHIGTRNPNNNAATVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRGNSRNSTPGSSRGNSPARMASGGGETALALLLLDRLNQLESKVSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKQYNVTQAFGRRGPEQTQGNFGDQDLIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYHGAIKLDDKDPQFKDNVILLNKHIDAYKTFPPTEPKKDKKKKTDEAQPLPQRQKKQPTVTLLPAADMDDFSRQLQNSMSGASADSTQA mint Major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid n-61 3716383 N/A 3716386 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified 248-365 binding region 248-365 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false 45-365 binding region 45-365 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig 4a n-62 3716384 N/A 3716386 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role neutral component neutral component purified purified 248-365 binding region 248-365 binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3716386 weight by staining molecular weight estimation by staining weight by staining unspecified role unspecified role neutral component neutral component purified purified 248-365 binding region 248-365 binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig 4b n-63 3716385 N/A 3716386 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified 248-365 binding region 248-365 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false 45-365 binding region 45-365 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig 4c