IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ li-2005-15 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip autoradiography autoradiography Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus There no entry in NCBI nor Uniprot databases for the SZ3 strain Spike protein isolated from the Palm Civet; thus this protein has not been entered here. Interactions involving chimeric proteins were not captured in accordance to IMEx curation rules. yguan@hkucc.hku.hk,hyeryun.choe@tch.harvard.edu The EMBO journal 2005 Only protein-protein interactions imex curation imex curation li-2005-13 in vitro In vitro facs fluorescence-activated cell sorting FACS Flow cytometry facs antibody detection identification by antibody antibody detection Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus There no entry in NCBI nor Uniprot databases for the SZ3 strain Spike protein isolated from the Palm Civet; thus this protein has not been entered here. Interactions involving chimeric proteins were not captured in accordance to IMEx curation rules. yguan@hkucc.hku.hk,hyeryun.choe@tch.harvard.edu The EMBO journal 2005 Only protein-protein interactions imex curation imex curation li-2005-12 in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom autoradiography autoradiography Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus There no entry in NCBI nor Uniprot databases for the SZ3 strain Spike protein isolated from the Palm Civet; thus this protein has not been entered here. Interactions involving chimeric proteins were not captured in accordance to IMEx curation rules. yguan@hkucc.hku.hk,hyeryun.choe@tch.harvard.edu The EMBO journal 2005 Only protein-protein interactions imex curation imex curation li-2005-14 in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus There no entry in NCBI nor Uniprot databases for the SZ3 strain Spike protein isolated from the Palm Civet; thus this protein has not been entered here. Cleavage of the peptide by ACE2 results in increased fluorescence due to the removal of the 2,4-dinitrophenyl moiety that quenches the fluorescence of the 7-methoxycoumarin moiety. Interactions involving chimeric proteins were not captured in accordance to IMEx curation rules. yguan@hkucc.hku.hk,hyeryun.choe@tch.harvard.edu The EMBO journal 2005 Only protein-protein interactions imex curation imex curation li-2005-16 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus There no entry in NCBI nor Uniprot databases for the SZ3 strain Spike protein isolated from the Palm Civet; thus this protein has not been entered here. Interactions involving chimeric proteins were not captured in accordance to IMEx curation rules. yguan@hkucc.hku.hk,hyeryun.choe@tch.harvard.edu The EMBO journal 2005 Only protein-protein interactions imex curation imex curation ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses spike_sars Spike glycoprotein S E2 Peplomer protein 2 protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFGNPVIPFKDGIYFAATEKSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEYISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDIWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNASSEVAVLYQDVNCTDVSTAIHADQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSLLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCTQLNRALSGIAAEQDRNTREVFAQVKQMYKTPTLKYFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADAGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGNCDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVWLGFIAGLIAIVMVTILLCCMTSCCSCLKGACSCGSCCKFDEDDSEPVLKGVKLHYT ace2_pagla Angiotensin-converting enzyme 2 ACE2 ACE-related carboxypeptidase protein protein Masked palm civet Paguma larvata MSGSFWLLLSFAALTAAQSTTEELAKTFLETFNYEAQELSYQSSVASWNYNTNITDENAKNMNEAGAKWSAYYEEQSKLAQTYPLAEIQDAKIKRQLQALQQSGSSVLSADKSQRLNTILNAMSTIYSTGKACNPNNPQECLLLEPGLDNIMENSKDYNERLWAWEGWRAEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWTGGYNYSRNQLIQDVEDTFEQIKPLYQHLHAYVRAKLMDTYPSRISRTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTDAMVNQNWDARRIFKEAEKFFVSVGLPNMTQGFWENSMLTEPGDGRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKTIGLLSPAFSEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGAIPKEQWMQKWWEMKRNIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCQIAKHEGPLHKCDISNSTEAGKKLLEMLSLGRSEPWTLALERVVGAKNMNVTPLLNYFEPLFTWLKEQNRNSFVGWDTDWRPYSDQSIKVRISLKSALGEKAYEWNDNEMYLFRSSIAYAMREYFSKVKNQTIPFVEDNVWVSDLKPRISFNFFVTFSNNVSDVIPRSEVEDAIRMSRSRINDAFRLDDNSLEFLGIEPTLSPPYRPPVTIWLIVFGVVMGAIVVGIVLLIVSGIRNRRKNDQAGSEENPYASVDLNKGENNPGFQHADDVQTSF q5gdb5_sars Spike glycoprotein S E2 Peplomer protein protein protein SARS coronavirus PC4-145 SARS coronavirus PC4-145 MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFDNPVIPFKDGIYFAATEKSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFVVSKPMGTRTHTMIFDNAFNCTFEYISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDTWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKRISNCVADYSVLYNSTSFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPAPNCYWPLNGYGFYTTSGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNASSEVAVLYQDVNCTDVSTLIHAEQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSSLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCRQLNRALSGIAAEQDRNTREVFVQVKQMYKTPTLKDFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADAGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGNCDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQEEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVWLGFIAGLIAIVMVTILLCCMTSCCSCLKGACSCGSCCKFDEDDSEPVLKGVKLHYT ace2_rat Angiotensin-converting enzyme 2 Ace2 ACE-related carboxypeptidase protein protein rat Rattus norvegicus (Rat) Rat MSSSCWLLLSLVAVATAQSLIEEKAESFLNKFNQEAEDLSYQSSLASWNYNTNITEENAQKMNEAAAKWSAFYEEQSKIAQNFSLQEIQNATIKRQLKALQQSGSSALSPDKNKQLNTILNTMSTIYSTGKVCNSMNPQECFLLEPGLDEIMATSTDYNRRLWAWEGWRAEVGKQLRPLYEEYVVLKNEMARANNYEDYGDYWRGDYEAEGVEGYNYNRNQLIEDVENTFKEIKPLYEQLHAYVRTKLMEVYPSYISPTGCLPAHLLGDMWGRFWTNLYPLTTPFLQKPNIDVTDAMVNQSWDAERIFKEAEKFFVSVGLPQMTPGFWTNSMLTEPGDDRKVVCHPTAWDLGHGDFRIKMCTKVTMDNFLTAHHEMGHIQYDMAYAKQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLPSNFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFQDKIPREQWTKKWWEMKREIVGVVEPLPHDETYCDPASLFHVSNDYSFIRYYTRTIYQFQFQEALCQAAKHDGPLHKCDISNSTEAGQKLLNMLSLGNSGPWTLALENVVGSRNMDVKPLLNYFQPLFVWLKEQNRNSTVGWSTDWSPYADQSIKVRISLKSALGKNAYEWTDNEMYLFRSSVAYAMREYFSREKNQTVPFGEADVWVSDLKPRVSFNFFVTSPKNVSDIIPRSEVEEAIRMSRGRINDIFGLNDNSLEFLGIYPTLKPPYEPPVTIWLIIFGVVMGTVVVGIVILIVTGIKGRKKKNETKREENPYDSMDIGKGESNAGFQNSDDAQTSF peptide-mca-yvadapk-dnp 7-methoxycoumarin-YVADAPK(2,4-dinitrophenyl)-OH peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro YVADAPK s-ace2-11 3682215 N/A 3682220 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate C9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 3682221 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate P59594:p.Lys344Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P59594:p.Phe360Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P59594:p.Asn479Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P59594:p.Thr487Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false Fig. 5B, Fig. 6B s-ace2-12 3682215 N/A 3682222 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate in vitro translated in vitro translated protein in vitro translated C9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 3682221 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate P59594:p.Lys344Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P59594:p.Phe360Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P59594:p.Asn479Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P59594:p.Thr487Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false Fig. 5B, Fig. 6B Also SZ3 (genebank AY304486) binds to civet ACE2 ace2-s-20 3682215 N/A 3682220 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed cell lysate cell lysate C9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q9BYF1:p.[Asp30_Phe40delinsGluThrPheAsnTyrGluAlaGlnGluLeuSer;Asn90_Val93delinsAspAlaLysIle] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Asn90_Val93delinsAspAlaLysIle mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Asp30_Phe40delinsGluThrPheAsnTyrGluAlaGlnGluLeuSer mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 3682221 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed cell lysate cell lysate P59594:p.Asn479Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P59594:p.[Asn479Lys;Thr487Ser] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P59594:p.Thr487Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false Fig. 7C mutant effects were determined in respect to WT binding partner. ace2-s-21 3682215 N/A 3682221 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed cell lysate cell lysate P59594:p.Asn479Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P59594:p.[Asn479Lys;Thr487Ser] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P59594:p.Thr487Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 3682222 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed cell lysate cell lysate c9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false Fig. 7C s-ace2-17 3682215 N/A 3682222 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate in vitro translated in vitro translated protein in vitro translated C9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 3682223 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q5GDB5:p.Asn78Asp mutation mutation certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false Fig. 5B Also SZ3 (genebank AY304486) binds to civet ACE2 s-ace2-18 3682215 N/A 3682220 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate in vitro translated in vitro translated protein in vitro translated C9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 3682223 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q5GDB5:p.Asn78Asp mutation mutation certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false Fig. 5B Also SZ3 (genebank AY304486) binds to civet ACE2 weak interaction s-ace2-7 3682216 N/A 3682220 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed cell lysate cell lysate C9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9BYF1:p.Lys353His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Met82_Pro84delinsAsnPheSer mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Lys353Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Lys353Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q9BYF1:p.[Asp30_Phe40delinsGluThrPheAsnTyrGluAlaGlnGluLeuSer;Asn90_Val93delinsAspAlaLysIle] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Asn90_Val93delinsAspAlaLysIle mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Asp30_Phe40delinsGluThrPheAsnTyrGluAlaGlnGluLeuSer mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 3682221 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed cell lysate cell lysate human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false S1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false Fig. 2A, Fig. 7A Figure 7A shows that the S1 domains of GD and SZ3 bound substantially more efficiently when either of these regions was introduced into human ACE2, and that introduction of both regions resulted in receptor binding comparable to that with wild-type palm-civet ACE2. s-ace2-8 3682216 N/A 3682221 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false S1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 3682224 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate Q5EGZ1:p.Asn82_Ser84delinsMetTyrPro mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain false C9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q5EGZ1:p.His353Lys mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain false 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q5EGZ1:p.[Asn82_Ser84delinsMetTyrPro;His353Lys] mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false Fig. 2A ace2-s-19 3682216 N/A 3682221 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed cell lysate cell lysate human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false S1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 3682222 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed cell lysate cell lysate c9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false Fig. 7A ace2-dgspike-1 3682216 N/A 3682220 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed cell lysate cell lysate C9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q9BYF1:p.[Asp30_Phe40delinsGluThrPheAsnTyrGluAlaGlnGluLeuSer;Asn90_Val93delinsAspAlaLysIle] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Asn90_Val93delinsAspAlaLysIle mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Asp30_Phe40delinsGluThrPheAsnTyrGluAlaGlnGluLeuSer mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 3682223 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed cell lysate cell lysate human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false S1 sufficient to bind sufficient binding region sufficient to bind range range range range false Q5GDB5:p.Asn78Asp mutation mutation certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false Fig. 7A Figure 7A shows that the S1 domains of GD and SZ3 bound substantially more efficiently when either of these regions was introduced into human ACE2, and that introduction of both regions resulted in receptor binding comparable to that with wild-type palm-civet ACE2. spike-ace2-1 3682217 N/A 3682220 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9BYF1:p.Lys353His mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Pro84Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Glu110Pro mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Pro135_Asp136delinsSerMet mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Glu160Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.[His228Asn;Glu231Lys] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.His239Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Arg306Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.[Asn322Gln;Gln325Pro;Glu329Thr] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Asn338_Gln340delinsAspAspArg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Leu359Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Ser425_Asp427delinsProSerAsn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Lys465_Glu467delinsGlnAspLys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Arg393Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Lys31Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false c9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q9BYF1:p.[Gln24Lys;Lys26Glu] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Met82_Pro84delinsAsnPheSer mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Arg559Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.[Arg559Ser;Lys562Asn;Glu564Gly] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Phe603Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Lys353Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Lys353Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q9BYF1:p.Tyr41Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Asp355Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Arg357Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Lys68Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Met383Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Pro389Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Glu37Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Asp38Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Lys309Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Glu312Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Thr324Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Asp350Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Leu359Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Arg192Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Arg219Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 3682221 unspecified role unspecified role bait bait purified purified human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false S1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false Fig. 1, Fig. 2A-B, Fig. 3A, Fig.4B s-ace2-6 3682217 N/A 3682221 unspecified role unspecified role bait bait purified purified human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false S1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 3682224 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate c9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q5EGZ1:p.[Asn82_Ser84delinsMetTyrPro;His353Lys] mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false Fig. 2A-B ace2-peptide-1 3682218 N/A 3682220 enzyme enzyme neutral component neutral component purified purified ecd_region sufficient to bind sufficient binding region sufficient to bind range range range range false N/A 3682225 enzyme target enzyme target substrate substrate neutral component neutral component purified purified region dnp dnp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region fluorophore fluorophore undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) cleavage cleavage reaction cleavage false false false Fig. 4A MLN-4760-an ACE2 inhibitor. ace2-spike-1 3682219 N/A 3682220 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range false N/A 3682221 unspecified role unspecified role bait bait purified purified RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human IgG fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P59594:p.Asn479Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P59594:p.Lys344Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P59594:p.Phe360Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P59594:p.Thr487Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 3682219 Table 1, Fig. S2