IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ shimada-2008-1 Chk1 is a histone H3 threonine 11 kinase that regulates DNA damage-induced transcriptional repression. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Shimada M., Niida H., Zineldeen DH., Tagami H., Tanaka M., Saito H., Nakanishi M. Chromatin - Epigenetic interactions resulting in chromatin modulation mkt-naka@med.nagoya-cu.ac.jp Cell (0092-8674) 2008 Only protein-protein interactions 1 imex curation gcn5_yeast Histone acetyltransferase GCN5 YGR252W GCN5 ADA4 SWI9 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVTKHQIEEDHLDGATTDPEVKRVKLENNVEEIQPEQAETNKQEGTDKENKGKFEKETERIGGSEVVTDVEKGIVKFEFDGVEYTFKERPSVVEENEGKIEFRVVNNDNTKENMMVLTGLKNIFQKQLPKMPKEYIARLVYDRSHLSMAVIRKPLTVVGGITYRPFDKREFAEIVFCAISSTEQVRGYGAHLMNHLKDYVRNTSNIKYFLTYADNYAIGYFKKQGFTKEITLDKSIWMGYIKDYEGGTLMQCSMLPRIRYLDAGKILLLQEAALRRKIRTISKSHIVRPGLEQFKDLNNIKPIDPMTIPGLKEAGWTPEMDALAQRPKRGPHDAAIQNILTELQNHAAAWPFLQPVNKEEVPDYYDFIKEPMDLSTMEIKLESNKYQKMEDFIYDARLVFNNCRMYNGENTSYYKYANRLEKFFNNKVKEIPEYSHLID 3200730DDC7EF70D h3_peptide-2-21 Aminoacid 2-21 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro ARTKQTARKSTGGKAPRKQLA An amino acid sequence which is not represented in UniProt Contains consensus aminoacids 1-21 shared between, human, bovin, mouse and variants of histone H3 from other organisms. chk1-hist1h3-2 Interaction between HIST1H3 and Chk1 demonstrated by SPR 728900 N/A 728901 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection N/A 728902 unspecified role unspecified role prey prey thr-12 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Kd wild type H3 tail peptide = 3.8E-7m in presence of 0.15mM CoA. In absence of CoA = 3.0E-4M Kd phosphorylated Thr-12 H3 tail peptide = 1.4E-8m in presence of 0.15mM CoA. In absence of CoA = 7.4E-4M. 6B Kd phosphorylated Ser-11 H3 tail peptide = 1.3E-8m in presence of 0.15mM CoA. Kd phosphorylated Ser-11/Thr-12 H3 tail peptide = 2.0E-8m in presence of 0.15mM CoA. In the absence of CoA, binding affinities between GCN5 and the peptide forms was significantly reduced.