IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ wang-2006e-2 Partially redundant functions of two SET-domain polycomb-group proteins in controlling initiation of seed development in Arabidopsis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Wang D., Tyson MD., Jackson SS., Yadegari R. Chromatin - Epigenetic interactions resulting in chromatin modulation Although not specifically stated in the text the participant detection method was presumed to be nucleotide sequence analysis of the isolated cDNA clones. Total RNA was isolated from Arabidopsis (Ler) open/anthesis-stage flowers (stage 14) and closed buds (stage 1 to 13 and inflorescence meristems) separately, and equal amounts of RNA from each sample were mixed for library construction. yadegari@ag.arizona.edu Arabidopsis reproductive organ cDNA library. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2006 wang-2006e-1 Partially redundant functions of two SET-domain polycomb-group proteins in controlling initiation of seed development in Arabidopsis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Wang D., Tyson MD., Jackson SS., Yadegari R. Chromatin - Epigenetic interactions resulting in chromatin modulation Although not specifically stated in the text the participant detection method was presumed to be nucleotide sequence analysis of the isolated cDNA clones. Total RNA was isolated from Arabidopsis (Ler) open/anthesis-stage flowers (stage 14) and closed buds (stage 1 to 13 and inflorescence meristems) separately, and equal amounts of RNA from each sample were mixed for library construction. yadegari@ag.arizona.edu Arabidopsis reproductive organ cDNA library. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2006 wang-2006e-3 Partially redundant functions of two SET-domain polycomb-group proteins in controlling initiation of seed development in Arabidopsis. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Wang D., Tyson MD., Jackson SS., Yadegari R. Chromatin - Epigenetic interactions resulting in chromatin modulation Although not specifically stated in the text the participant detection method was presumed to be nucleotide sequence analysis of the isolated cDNA clones. Total RNA was isolated from Arabidopsis (Ler) open/anthesis-stage flowers (stage 14) and closed buds (stage 1 to 13 and inflorescence meristems) separately, and equal amounts of RNA from each sample were mixed for library construction. yadegari@ag.arizona.edu Arabidopsis reproductive organ cDNA library. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2006 fis2_arath_protein fis2 protein long isoform protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress The protein could not be merged with EBI-1102026 because of some conflicts with the protein sequence (features which cannot be shifted). The authors state "Our data indicate that FIS2 should encode a protein of 813 aa, which is 121 aa longer than was reported as a full-length FIS2 product (9)" PMID:16924116 medea_arath Histone-lysine N-methyltransferase MEDEA EMB173 Maternal embryogenesis control protein Protein FERTILIZATION-INDEPENDENT SEED 1 Protein SET DOMAIN GROUP 5 Protein EMBRYO DEFECTIVE 173 MEA FIS1 At1g02580 T14P4.11 MEDEA SDG5 SET5 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEKENHEDDGEGLPPELNQIKEQIEKERFLHIKRKFELRYIPSVATHASHHQSFDLNQPAAEDDNGGDNKSLLSRMQNPLRHFSASSDYNSYEDQGYVLDEDQDYALEEDVPLFLDEDVPLLPSVKLPIVEKLPRSITWVFTKSSQLMAESDSVIGKRQIYYLNGEALELSSEEDEEDEEEDEEEIKKEKCEFSEDVDRFIWTVGQDYGLDDLVVRRALAKYLEVDVSDILERYNELKLKNDGTAGEASDLTSKTITTAFQDFADRRHCRRCMIFDCHMHEKYEPESRSSEDKSSLFEDEDRQPCSEHCYLKVRSVTEADHVMDNDNSISNKIVVSDPNNTMWTPVEKDLYLKGIEIFGRNSCDVALNILRGLKTCLEIYNYMREQDQCTMSLDLNKTTQRHNQVTKKVSRKSSRSVRKKSRLRKYARYPPALKKTTSGEAKFYKHYTPCTCKSKCGQQCPCLTHENCCEKYCGCSKDCNNRFGGCNCAIGQCTNRQCPCFAANRECDPDLCRSCPLSCGDGTLGETPVQIQCKNMQFLLQTNKKILIGKSDVHGWGAFTWDSLKKNEYLGEYTGELITHDEANERGRIEDRIGSSYLFTLNDQLEIDARRKGNEFKFLNHSARPNCYAKLMIVRGDQRIGLFAERAIEEGEELFFDYCYGPEHADWSRGREPRKTGASKRSKEARPAR eza1_arath Histone-lysine N-methyltransferase EZA1 EZA1 SDG10 SET10 CURLY LEAF-like 1 Protein SET DOMAIN GROUP 10 Protein SWINGER SWN T10M13.3 At4g02020 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVTDDSNSSGRIKSHVDDDDDGEEEEDRLEGLENRLSELKRKIQGERVRSIKEKFEANRKKVDAHVSPFSSAASSRATAEDNGNSNMLSSRMRMPLCKLNGFSHGVGDRDYVPTKDVISASVKLPIAERIPPYTTWIFLDRNQRMAEDQSVVGRRQIYYEQHGGETLICSDSEEEPEPEEEKREFSEGEDSIIWLIGQEYGMGEEVQDALCQLLSVDASDILERYNELKLKDKQNTEEFSNSGFKLGISLEKGLGAALDSFDNLFCRRCLVFDCRLHGCSQPLISASEKQPYWSDYEGDRKPCSKHCYLQLKAVREVPETCSNFASKAEEKASEEECSKAVSSDVPHAAASGVSLQVEKTDIGIKNVDSSSGVEQEHGIRGKREVPILKDSNDLPNLSNKKQKTAASDTKMSFVNSVPSLDQALDSTKGDQGGTTDNKVNRDSEADAKEVGEPIPDNSVHDGGSSICQPHHGSGNGAIIIAEMSETSRPSTEWNPIEKDLYLKGVEIFGRNSCLIARNLLSGLKTCLDVSNYMRENEVSVFRRSSTPNLLLDDGRTDPGNDNDEVPPRTRLFRRKGKTRKLKYSTKSAGHPSVWKRIAGGKNQSCKQYTPCGCLSMCGKDCPCLTNETCCEKYCGCSKSCKNRFRGCHCAKSQCRSRQCPCFAAGRECDPDVCRNCWVSCGDGSLGEAPRRGEGQCGNMRLLLRQQQRILLGKSDVAGWGAFLKNSVSKNEYLGEYTGELISHHEADKRGKIYDRANSSFLFDLNDQYVLDAQRKGDKLKFANHSAKPNCYAKVMFVAGDHRVGIFANERIEASEELFYDYRYGPDQAPVWARKPEGSKKDDSAITHRRARKHQSH clf_arath Histone-lysine N-methyltransferase CLF CLF At2g23380 F26B6.3 ICU1 PIF1 PIF2 SDG1 SET1 Protein INCURVATA 1 Protein photoperiod insensitive flowering Protein SET DOMAIN GROUP 1 Polycomb group protein CURLY LEAF protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASEASPSSSATRSEPPKDSPAEERGPASKEVSEVIESLKKKLAADRCISIKKRIDENKKNLFAITQSFMRSSMERGGSCKDGSDLLVKRQRDSPGMKSGIDESNNNRYVEDGPASSGMVQGSSVPVKISLRPIKMPDIKRLSPYTTWVFLDRNQRMTEDQSVVGRRRIYYDQTGGEALICSDSEEEAIDDEEEKRDFLEPEDYIIRMTLEQLGLSDSVLAELASFLSRSTSEIKARHGVLMKEKEVSESGDNQAESSLLNKDMEGALDSFDNLFCRRCLVFDCRLHGCSQDLIFPAEKPAPWCPPVDENLTCGANCYKTLLKSGRFPGYGTIEGKTGTSSDGAGTKTTPTKFSSKLNGRKPKTFPSESASSNEKCALETSDSENGLQQDTNSDKVSSSPKVKGSGRRVGRKRNKNRVAERVPRKTQKRQKKTEASDSDSIASGSCSPSDAKHKDNEDATSSSQKHVKSGNSGKSRKNGTPAEVSNNSVKDDVPVCQSNEVASELDAPGSDESLRKEEFMGETVSRGRLATNKLWRPLEKSLFDKGVEIFGMNSCLIARNLLSGFKSCWEVFQYMTCSENKASFFGGDGLNPDGSSKFDINGNMVNNQVRRRSRFLRRRGKVRRLKYTWKSAAYHSIRKRITEKKDQPCRQFNPCNCKIACGKECPCLLNGTCCEKYCGCPKSCKNRFRGCHCAKSQCRSRQCPCFAADRECDPDVCRNCWVIGGDGSLGVPSQRGDNYECRNMKLLLKQQQRVLLGISDVSGWGAFLKNSVSKHEYLGEYTGELISHKEADKRGKIYDRENCSFLFNLNDQFVLDAYRKGDKLKFANHSPEPNCYAKVIMVAGDHRVGIFAKERILAGEELFYDYRYEPDRAPAWAKKPEAPGSKKDENVTPSVGRPKKLA fis2_arath Polycomb group protein FERTILIZATION-INDEPENDENT SEED 2 FIS2 At2g35670 T20F21.14 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MARKSIRGKEVVMVSDDDDDDDDDVDDDKNIIKCVKPLTVYKNLETPTDSDDNDDDDDDDVDVDENIIKYIKPVAVYKKLETRSKNNPYFLRRSLKYIIQAKKKKKSNSGGKIRFNYRDVSNKMTLKAEVVENFSCPFCLIPCGGHEGLQLHLKSSHDAFKFEFYRAEKDHGPEVDVSVKSDTIKFGVLKDDVGNPQLSPLTFCSKNRNQRRQRDDSNNVKKLNVLLMELDLDDLPRGTENDSTHVNDDNVSSPPRAHSSEKISDILTTTQLAIAESSEPKVPHVNDGNVSSPPRAHSSAEKNESTHVNDDDDVSSPPRAHSLEKNESTHVNEDNISSPPKAHSSKKNESTHMNDEDVSFPPRTRSSKETSDILTTTQPAIVEPSEPKVRRVSRRKQLYAKRYKARETQPAIAESSEPKVLHVNDENVSSPPEAHSLEKASDILTTTQPAIAESSEPKVPHVNDENVSSTPRAHSSKKNKSTRKNVDNVPSPPKTRSSKKTSDILTTTQPTIAESSEPKVRHVNDDNVSSTPRAHSSKKNKSTRKNDDNIPSPPKTRSSKKTSNILATTQPAKAEPSEPKVTRVSRRKELHAERCEAKRLERLKGRQFYHSQTMQPITFEQVMSNEDSENETDDYALDISERLRLERLVGVSKEEKRYMYLWNIFVRKQRVIADGHVPWACEEFAKLHKEEMKNSSSFDWWWRMFRIKLWNNGLICAKTFHKCTTILLSNSDEAGQFTSGSAANANNQQSMEVDE fis2-mea-1 542554 n/a 542557 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 542558 unspecified role unspecified role prey prey physical association physical association false false false 1B fis2-eza1-1 542554 n/a 542557 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 542559 unspecified role unspecified role prey prey physical association physical association false false false 1B fis2-clf 542554 n/a 542557 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 542560 unspecified role unspecified role prey prey physical association physical association false false false 1B fis2-mea 542555 n/a 542561 unspecified role unspecified role bait bait N/A 542558 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false fis2-eza1 542555 n/a 542561 unspecified role unspecified role bait bait N/A 542559 unspecified role unspecified role prey prey physical association physical association false false false fis2-eza1-2 542556 n/a 542557 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 542559 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false 1C fis2-mea-2 542556 n/a 542557 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 542558 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false 1C