IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ el_assal-2004-2 Arabidopsis GNARLED encodes a NAP125 homolog that positively regulates ARP2/3. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined El-Assal Sel-D., Le J., Basu D., Mallery EL., Szymanski DB. Chromatin - Epigenetic interactions resulting in chromatin modulation dszyman@purdue.edu Curr. Biol. (0960-9822) 2004 el_assal-2004-1 Arabidopsis GNARLED encodes a NAP125 homolog that positively regulates ARP2/3. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot El-Assal Sel-D., Le J., Basu D., Mallery EL., Szymanski DB. Chromatin - Epigenetic interactions resulting in chromatin modulation dszyman@purdue.edu Curr. Biol. (0960-9822) 2004 nckp1_arath Protein NAP1 Nck-associated protein 1 p125Nap1 NAP of plants ARP2/3 regulatory protein subunit NAPP Protein GNARLED NAP1 GRL NAP NAPP T4C15.22 At2g35110 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MANSRQYYPSQDESMSPTSVRSREWEGPSRWTEYLGPEMAASVSSTRSSKQIDGHVGGSTKALNIQWVVQMIEVADGLMAKMYRLNQILEYPDPVGHVFSEAFWKAGVFPNHPRICTLLSKKFPEHFSKLQLERIDKFSLDSLHDGAELHLQSLEPWIQLLLDLMAFREQALRLILDLSSTVITLLPHQNSLILHAFMDLFCAFVRVNLFAEKIPRKMLLQVYNLLHALSRNDRDCDFYHRLVQFIDSYDPPLKGLQEDLNFVSPRIGEVLEAVGPSIFLSADTRKLRNEGFLSPYHPRFPDILTNSAHPMRAQDLANVTSYREWVLLGYLVCPDELLRVTSIDIALVVLKENLVVTLFRDEVSLYQMVCEKEFGIGISFASADSINLTMQYILLHEDYQLYVLPRVLESKKMAKSGRTKQKEADLEYSVAKQVEKMISEVHEQALQLCDTIHRERRILLKQEIGRMVLFFTDQPSLLAPNIQMVFSALALAQSEVLWYFQHAGIASSRSKAARVIPVDIDPNDPTIGFLLDGMDRLCCLVRKYISAARGYALSYLSSSAGRIRYLMGTPGIVALDLDPTLKGLFQRIVQHLESIPKAQGENVSAITCDLSDFRKDWLSILMIVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESQLSKHGSLKKLYFYHQHLTTVFRNTMFGPEGRPQHCCAWLSVASSFPECASLIIPEEVTKFGRDAVLYVESLIESIMGGLEGLINILDSEGGFGALESQLLPEQAAAYLNNASRISAPSVKSPRVVGGFTLPGHESYPENNKSIKMLEAAIQRLTNLCSILNDMEPICVINHVFVLREYMRECILGNFKRRFLTALQTDNDLQRPSVLESLIRRHMGIVHLAEQHVSMDLTQGIREILLTEAFSGPVSSLHTFEKPAEQQQTTGSAVEVVCNWYMDNIIKDVSGAGILFAPRHKYFKSTRPVGGYFAESVTDLKELQAFVRIFGGYGVDRLDRMMKVHTAALVNCIETSLRSNRELIEAAAASMHSGDRVERDASVRQIVDLDTVIGFCIEAGQALAFDDLLAEASGAVLEDNASLIHSMISGIVEHIPEEIPEKKEIRRIKGVANGVGVAGDHDSEWVRLILEEVGGANDNSWSLLPYFFASFMTSNAWNTTGFNIETGGFSNNIHCLARCISAVIAGSEYVRLQREYQQQHQSLSNGHHSSENLDSEFPPRVTAEASIKSSMLLFVKFAASIVLDSWSEANRSHLVAKLIFLDQLCEISPYLPRSSLESHVPYTILRSIYTQYYSNTPSTPLSTASPYHSPSVSLIHASPSMKNSTTPQRGSGSGSSSTAAPDSGYFKGSSSSLYGQEHYTESETGNSRNNENNNNNKQRGSSRRSGPLDYSSSHKGGSGSNSTGPSPLPRFAVSRSGPISYKQHN pir_arath Protein PIR PIR of plants Protein PIROGI Protein KLUNKER PIR KLK PIRP F20L16.130 At5g18410 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAVPVEEAIAALSTFSLEDEQPEVQGPAVMVSAERAATDSPIEYSDVAAYRLSLSEDTKALNQLNTLIQEGKEMASILYTYRSCVKALPQLPESMKHSQADLYLETYQVLDLEMSRLREIQRWQSSASAKLAADMQRFSRPERRINGPTVTHLWSMLKLLDVLVQLDHLKNAKASIPNDFSWYKRTFTQVSAQWQDTDTMREELDDLQIFLSTRWAILLNLHVEMFRVNNVEDILQVLIVFIVESLELDFALLFPERYILLRVLPVLVVLATPSEKDTEALYKRVKLNRLINIFKNDPVIPAFPDLHLSPAAILKELSVYFQKFSSQTRLLTLPAPHELPPREALEYQRHYLIVNHIGALRAEHDDFTIRFASSMNQLLLLKSNDGAYTEWCREVKGNMYDMVVEGFQLLSRWTARIWEQCAWKFSRPCRDAGETPEASGSYSDYEKVVRFNYTAEERKALVELVGYIKSVGSMLQRCDTLVADALWETIHAEVQDFVQNTLATMLRTTFRKKKDLSRILSDMRTLSADWMANTRPEHEMPSSQHGNDESRGNFFYPRPVAPTAAQVHCLQFLIYEVVSGGNLRRPGGFFGNNGSEIPVNDLKQLETFFYKLSFFLHILDYSASIGILTDLGFLWFREFYLESSRVIQFPIECSLPWMLIDYILEAQNSGLLESVLLPFDIYNDSAQQALVVLRQRFLYDEIEAEVDHGFDIFVSRLSESIFTYYKSWSASELLDPSFLFALDNGEKFSIQPVRFTALFKMTKVKILGRTINLRSLIAQRMNRIFRENLEFLFDRFESQDLCAVVELEKLIDILKHSHELLSQDLSIDPFSLMLNEMQENISLVSFSSRLATQIWSEMQSDFLPNFILCNTTQRFVRSSKVPPTQKPSVPSAKPSFYCGTQDLNAAHQSFARLHSGFFGIPHLFSIVKLLGSRSLPWLIRALLDHISNKITTLEPMISGLQEALPKSIGLLSFDGGVTGCMKLIREQLNWGTKSELKSEVLRGIKEIGSVIYTMGLLDIVLREVDTKRFMQTAPWLGLIPGAEGQIVNAQDGESPLVNLLKSATSAVVSSPGCLNPAAFYTMSKQAEAADLLYKANMNGGSVLEYTLAFTSASLDKYCSKWSAPPKTGFVDITTSKDFYRIYGGLQIGYLEEITAPQSAQHEVLGDSIAWGGCTIIYLLGQQLHFELFDFSYQVLNVSEVETVSASHTHRNPQIHQGWEGLLEGMKKARRLNNHVFSMLKARCPLEDKTACAIKQSGAPLPRVRFENTVSAFETLPQKGTVG nckp1_human Nck-associated protein 1 NCKAP1 HEM2 KIAA0587 NAP1 p125Nap1 Membrane-associated protein HEM-2 protein protein human Homo sapiens Human MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA cyfp1_human Cytoplasmic FMR1-interacting protein 1 Specifically Rac1-associated protein 1 p140sra-1 CYFIP1 KIAA0068 protein protein human Homo sapiens Human MAAQVTLEDALSNVDLLEELPLPDQQPCIEPPPSSLLYQPNFNTNFEDRNAFVTGIARYIEQATVHSSMNEMLEEGQEYAVMLYTWRSCSRAIPQVKCNEQPNRVEIYEKTVEVLEPEVTKLMNFMYFQRNAIERFCGEVRRLCHAERRKDFVSEAYLITLGKFINMFAVLDELKNMKCSVKNDHSAYKRAAQFLRKMADPQSIQESQNLSMFLANHNKITQSLQQQLEVISGYEELLADIVNLCVDYYENRMYLTPSEKHMLLKVMGFGLYLMDGSVSNIYKLDAKKRINLSKIDKYFKQLQVVPLFGDMQIELARYIKTSAHYEENKSRWTCTSSGSSPQYNICEQMIQIREDHMRFISELARYSNSEVVTGSGRQEAQKTDAEYRKLFDLALQGLQLLSQWSAHVMEVYSWKLVHPTDKYSNKDCPDSAEEYERATRYNYTSEEKFALVEVIAMIKGLQVLMGRMESVFNHAIRHTVYAALQDFSQVTLREPLRQAIKKKKNVIQSVLQAIRKTVCDWETGHEPFNDPALRGEKDPKSGFDIKVPRRAVGPSSTQLYMVRTMLESLIADKSGSKKTLRSSLEGPTILDIEKFHRESFFYTHLINFSETLQQCCDLSQLWFREFFLELTMGRRIQFPIEMSMPWILTDHILETKEASMMEYVLYSLDLYNDSAHYALTRFNKQFLYDEIEAEVNLCFDQFVYKLADQIFAYYKVMAGSLLLDKRLRSECKNQGATIHLPPSNRYETLLKQRHVQLLGRSIDLNRLITQRVSAAMYKSLELAIGRFESEDLTSIVELDGLLEINRMTHKLLSRYLTLDGFDAMFREANHNVSAPYGRITLHVFWELNYDFLPNYCYNGSTNRFVRTVLPFSQEFQRDKQPNAQPQYLHGSKALNLAYSSIYGSYRNFVGPPHFQVICRLLGYQGIAVVMEELLKVVKSLLQGTILQYVKTLMEVMPKICRLPRHEYGSPGILEFFHHQLKDIVEYAELKTVCFQNLREVGNAILFCLLIEQSLSLEEVCDLLHAAPFQNILPRVHVKEGERLDAKMKRLESKYAPLHLVPLIERLGTPQQIAIAREGDLLTKERLCCGLSMFEVILTRIRSFLDDPIWRGPLPSNGVMHVDECVEFHRLWSAMQFVYCIPVGTHEFTVEQCFGDGLHWAGCMIIVLLGQQRRFAVLDFCYHLLKVQKHDGKDEIIKNVPLKKMVERIRKFQILNDEIITILDKYLKSGDGEGTPVEHVRCFQPPIHQSLASS pir-nap1-1 PIR (SRA1) interacts with Nap1 (GRL) 735661 N/A 735663 unspecified role unspecified role bait bait N/A 735664 unspecified role unspecified role prey prey physical association physical association false false false Figure 4B sra1-nap-1 Interaction between human SRA1 (CYFIP1) and human NAP protein (NCKAP1) 735661 N/A 735665 unspecified role unspecified role prey prey N/A 735666 unspecified role unspecified role bait bait physical association physical association false false false 4B pir-nap1-2 Arabidopsis PIR (SRA1) interacts with Human Nap1 (NCKAP1) 735661 N/A 735665 unspecified role unspecified role prey prey N/A 735664 unspecified role unspecified role bait bait physical association physical association false false false Figure 4B pir-nap1 PIR (SRA1) interacts with Nap1 (GRL) 735662 N/A 735664 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobd Escherichia coli (strain B / BL21-DE3) N/A 735663 unspecified role unspecified role prey prey n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobd Escherichia coli (strain B / BL21-DE3) direct interaction direct interaction false false false Figure 4A