IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ espinas-2000-3 The GAGA factor of Drosophila interacts with SAP18, a Sin3-associated polypeptide. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Espinas ML., Canudas S., Fanti L., Pimpinelli S., Casanova J., Azorin F. Chromatin - Epigenetic interactions resulting in chromatin modulation fambmc@cid.csic.es EMBO Rep. (1469-221X) 2000 Only protein-protein interactions imex curation Drosophila 0 to 12 h embryonic cDNA library. espinas-2000-2 The GAGA factor of Drosophila interacts with SAP18, a Sin3-associated polypeptide. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Espinas ML., Canudas S., Fanti L., Pimpinelli S., Casanova J., Azorin F. Chromatin - Epigenetic interactions resulting in chromatin modulation fambmc@cid.csic.es EMBO Rep. (1469-221X) 2000 Only protein-protein interactions imex curation Drosophila 0 to 12 h embryonic cDNA library. espinas-2000-1 The GAGA factor of Drosophila interacts with SAP18, a Sin3-associated polypeptide. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Espinas ML., Canudas S., Fanti L., Pimpinelli S., Casanova J., Azorin F. Chromatin - Epigenetic interactions resulting in chromatin modulation fambmc@cid.csic.es Drosophila 0 to 12 h embryonic cDNA library. EMBO Rep. (1469-221X) 2000 Only protein-protein interactions imex curation q08605-2 Transcription factor GAGA C D E H GAGA-519a GAGA-519b Trl Adf-2 GAGA Nc70F TFGAGA Adh transcription factor 2 GAGA factor Neural conserved at 70F Trithorax-like protein CG33261 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSLPMNSLYSLTWGDYGTSLVSAIQLLRCHGDLVDCTLAAGGRSFPAHKIVLCAASPFLLDLLKNTPCKHPVVMLAGVNANDLEALLEFVYRGEVSVDHAQLPSLLQAAQCLNIQGLAPQTVTKDDYTTHSIQLQHMIPQHHDQDQLIATIATAPQQTVHAQVVEDIHHQGQILQATTQTNAAGQQQTIVTTDAAKHDQAVIQAFLPARKRKPRVKKMSPTAPKISKVEGMDTIMGTPTSSHGSGSVQQVLGENGAEGQLLSSTPIIKSEGQKVETIVTMDPNNMIPVTSANAATGEITPAQGATGSSGGNTSGVLSTPKAKRAKHPPGTEKPRSRSQSEQPATCPICYAVIRQSRNLRRHLELRHFAKPGVKKEKKSKSGNDTTLDSSMEMNTTAEGDNTVGSDGAGGAGSAGGQSSGTTPTRVISNAPQAAGAPAILAQGVLPQQQQQQQLQQQHQQHLTATLAGGGQAYIKHEGGGGGGTGQQQQQQAAQQQGMQNVIHIVGDQVFIPQQQQPQPQ Ref.2 (AAB81113/AAB81117) sequences are in conflict in position: 437:A->G. sap18_drome Histone deacetylase complex subunit SAP18 Bin1 SAP18 CG6046 18 kDa Sin3-associated polypeptide Bicoid-interacting protein 1 dSAP18 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MANVESMIVEEKTQVKQIDREKTCPMLLRVFCSTGRHHSVSEYMFGNVPTNELQIYTWQDATLHELTSLVRDVNPDTRKKGTYFDFAVVYPNFRSNHFQMREIGVTCTGQKGIDDNKTLAQAKFSIGDFLDISITPPNRLPPTARRQRPY q08605-1 Transcription factor GAGA F GAGA-581 Trl Adf-2 GAGA Nc70F TFGAGA Adh transcription factor 2 GAGA factor Neural conserved at 70F Trithorax-like protein CG33261 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSLPMNSLYSLTWGDYGTSLVSAIQLLRCHGDLVDCTLAAGGRSFPAHKIVLCAASPFLLDLLKNTPCKHPVVMLAGVNANDLEALLEFVYRGEVSVDHAQLPSLLQAAQCLNIQGLAPQTVTKDDYTTHSIQLQHMIPQHHDQDQLIATIATAPQQTVHAQVVEDIHHQGQILQATTQTNAAGQQQTIVTTDAAKHDQAVIQAFLPARKRKPRVKKMSPTAPKISKVEGMDTIMGTPTSSHGSGSVQQVLGENGAEGQLLSSTPIIKSEGQKVETIVTMDPNNMIPVTSANAATGEITPAQGATGSSGGNTSGVLSTPKAKRAKHPPGTEKPRSRSQSEQPATCPICYAVIRQSRNLRRHLELRHFAKPGVKKEKKTTSGKKSSSGSSGSGSGALSSSGSVPQVQTVQSLHTLQGVQVKKDPDAQQQQQQQQQQQQQQQQAMTVSGATGGQVQQQVQQVQQQVQQQQQQQQQQQQQLQHHQIIDSSGNITTATTSAQAAAAAQQQAAGQQQQLVAQSDGSESGAPLSIAQVQTLQGHQIIGNLNQVNMTDFQQQQPQQQQQQQQQQQQQQQQQQQTQQTL gaga_drome Transcription factor GAGA Trl GAGA TFGAGA Trithorax-like protein GAGA factor Adh transcription factor 2 Neural conserved at 70F Adf-2 Nc70F CG33261 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSLPMNSLYSLTWGDYGTSLVSAIQLLRCHGDLVDCTLAAGGRSFPAHKIVLCAASPFLLDLLKNTPCKHPVVMLAGVNANDLEALLEFVYRGEVSVDHAQLPSLLQAAQCLNIQGLAPQTVTKDDYTTHSIQLQHMIPQHHDQDQLIATIATAPQQTVHAQVVEDIHHQGQILQATTQTNAAGQQQTIVTTDAAKHDQAVIQAFLPARKRKPRVKKMSPTAPKISKVEGMDTIMGTPTSSHGSGSVQQVLGENGAEGQLLSSTPIIKSEGQKVETIVTMDPNNMIPVTSANAATGEITPAQGATGSSGGNTSGVLSTPKAKRAKHPPGTEKPRSRSQSEQPATCPICYAVIRQSRNLRRHLELRHFAKPGVKKEKKTTSGKKSSSGSSGSGSGALSSSGSVPQVQTVQSLHTLQGVQVKKDPDAQQQQQQQQQQQQQQQQAMTVSGATGGQVQQQVQQVQQQVQQQQQQQQQQQQQLQHHQIIDSSGNITTATTSAQAAAAAQQQAAGQQQQLVAQSDGSESGAPLSIAQVQTLQGHQIIGNLNQVNMTDFQQQQPQQQQQQQQQQQQQQQQQQQTQQTL gaga-sap18-2 594001 N/A 594004 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 594005 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 1C, 2B gaga-sap18-3 594001 N/A 594004 unspecified role unspecified role prey prey drome-sl2 Drosophila melanogaster sl1 cells sl2 drosophila sl12 cells N/A 594005 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 1d gaga-gaga-1 594001 N/A 594006 unspecified role unspecified role prey prey drome-sl2 Drosophila melanogaster sl1 cells sl2 drosophila sl12 cells N/A 594004 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 594004 unspecified role unspecified role bait bait poz domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) association association false false false 1d gaga-sap18-4 594001 N/A 594005 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) N/A 594004 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false 1C, 2B gaga-sap18-1 594002 N/A 594004 unspecified role unspecified role bait bait poz domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 594005 unspecified role unspecified role prey prey physical association physical association false false false 1B gaga-gaga 594002 N/A 594004 unspecified role unspecified role bait bait poz domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 594004 unspecified role unspecified role prey prey poz domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1b gaga-sap18 594003 N/A 594005 unspecified role unspecified role prey prey N/A 594007 unspecified role unspecified role bait bait poz domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false