IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ rea-2000-1 Regulation of chromatin structure by site-specific histone H3 methyltransferases. in vitro In vitro radiolabeled methyl methyltransferase radiometric assay radiolabeled methyl predetermined predetermined participant predetermined Rea S., Eisenhaber F., O'Carroll D., Strahl BD., Sun ZW., Schmid M., Opravil S., Mechtler K., Ponting CP., Allis CD., Jenuwein T. Chromatin - Epigenetic interactions resulting in chromatin modulation Nature (0028-0836) 2000 rea-2000-2 Regulation of chromatin structure by site-specific histone H3 methyltransferases. in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Rea S., Eisenhaber F., O'Carroll D., Strahl BD., Sun ZW., Schmid M., Opravil S., Mechtler K., Ponting CP., Allis CD., Jenuwein T. Chromatin - Epigenetic interactions resulting in chromatin modulation Nature (0028-0836) 2000 suv91_human Histone-lysine N-methyltransferase SUV39H1 SUV39H1 SUV39H KMT1A Suppressor of variegation 3-9 homolog 1 Position-effect variegation 3-9 homolog Histone H3-K9 methyltransferase 1 Lysine N-methyltransferase 1A protein protein human Homo sapiens Human MAENLKGCSVCCKSSWNQLQDLCRLAKLSCPALGISKRNLYDFEVEYLCDYKKIREQEYYLVKWRGYPDSESTWEPRQNLKCVRILKQFHKDLERELLRRHHRSKTPRHLDPSLANYLVQKAKQRRALRRWEQELNAKRSHLGRITVENEVDLDGPPRAFVYINEYRVGEGITLNQVAVGCECQDCLWAPTGGCCPGASLHKFAYNDQGQVRLRAGLPIYECNSRCRCGYDCPNRVVQKGIRYDLCIFRTDDGRGWGVRTLEKIRKNSFVMEYVGEIITSEEAERRGQIYDRQGATYLFDLDYVEDVYTVDAAYYGNISHFVNHSCDPNLQVYNVFIDNLDERLPRIAFFATRTIRAGEELTFDYNMQVDPVDMESTRMDSNFGLAGLPGSPKKRVRIECKCGTESCRKYLF h31_bovin Histone H3.1 protein protein bovin Bos taurus (Bovine) Bovine MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA suv91_mouse Histone-lysine N-methyltransferase SUV39H1 Suv39h1 Suv39h Suppressor of variegation 3-9 homolog 1 Position-effect variegation 3-9 homolog Histone H3-K9 methyltransferase 1 protein protein mouse Mus musculus Mouse MAENLKGCSVCCKSSWNQLQDLCRLAKLSCPALGVSKKNLYDFEVEYLCDYKKIREQEYYLVKWRGYPDSENTWEPRQNLKCIRVLKQFHKDLERELVRRHRRSKPPRHLDPNLANYLVQKAKQRRALQRWEQELNAKRSHLGRITVENEVDLDGPPRSFVYINEYRVGEGITLNQVAVGCECQDCLLAPTGGCCPGASLHKFAYNDQGQVRLKAGQPIYECNSRCCCGYDCPNRVVQKGIRYDLCIFRTNDGRGWGVRTLEKIRKNSFVMEYVGEIITSEEAERRGQIYDRQGATYLFDLDYVEDVYTVDAAYYGNISHFVNHSCDPNLQVYNVFIDNLDERLPRIAFFATRTIWAGEELTFDYNMQVDPVDMESTRMDSNFGLAGLPGSPKKRVRIECKCGTTACRKYLF clr4_schpo Histone-lysine N-methyltransferase, H3 lysine-9 specific clr4 SPBC428.08c kmt1 Histone H3-K9 methyltransferase Cryptic loci regulator 4 Lysine N-methyltransferase 1 Protein lysine methyltransferase clr4 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSPKQEEYEVERIVDEKLDRNGAVKLYRIRWLNYSSRSDTWEPPENLSGCSAVLAEWKRRKRRLKGSNSDSDSPHHASNPHPNSRQKHQHQTSKSVPRSQRFSRELNVKKENKKVFSSQTTKRQSRKQSTALTTNDTSIILDDSLHTNSKKLGKTRNEVKEESQKRELVSNSIKEATSPKTSSILTKPRNPSKLDSYTHLSFYEKRELFRKKLREIEGPEVTLVNEVDDEPCPSLDFQFISQYRLTQGVIPPDPNFQSGCNCSSLGGCDLNNPSRCECLDDLDEPTHFAYDAQGRVRADTGAVIYECNSFCSCSMECPNRVVQRGRTLPLEIFKTKEKGWGVRSLRFAPAGTFITCYLGEVITSAEAAKRDKNYDDDGITYLFDLDMFDDASEYTVDAQNYGDVSRFFNHSCSPNIAIYSAVRNHGFRTIYDLAFFAIKDIQPLEELTFDYAGAKDFSPVQSQKSQQNRISKLRRQCKCGSANCRGWLFG 53C3EC87BCBA51FF suv92_mouse Histone-lysine N-methyltransferase SUV39H2 Suv39h2 Suppressor of variegation 3-9 homolog 2 Histone H3-K9 methyltransferase 2 protein protein mouse Mus musculus Mouse MATARAKARGSEAGARCHRAPGPPPRPKARRTARRRRAETLTARRSRPSAGERRAGSQRAWSGAPRAAVFGDECARGALFKAWCVPCLVSLDTLQELCRKEKLTCKSIGITKRNLNNYEVEYLCDYKVAKGVEYYLVKWKGWPDSTNTWEPLRNLRCPQLLRQFSDDKKTYLAQERKCKAVNSKSLQPAIAEYIVQKAKQRIALQRWQDYLNRRKNHKGMIFVENTVDLEGPPLDFYYINEYRPAPGISINSEATFGCSCTDCFFDKCCPAEAGVVLAYNKKQQIKIQPGTPIYECNSRCRCGPECPNRIVQKGTQYSLCIFKTSNGCGWGVKTLVKIKRMSFVMEYVGEVITSEEAERRGQFYDNKGITYLFDLDYESDEFTVDAARYGNVSHFVNHSCDPNLQVFSVFIDNLDTRLPRIALFSTRTINAGEELTFDYQMKGSGEASSDSIDHSPAKKRVRTQCKCGAETCRGYLN h31_human Histone H3.1 H3FA H3FL H3FD H3FI H3FH H3FK H3FF H3FJ H3FB Histone H3/a Histone H3/b Histone H3/c Histone H3/d Histone H3/f Histone H3/h Histone H3/i Histone H3/j Histone H3/k Histone H3/l H3C1 HIST1H3A H3C2 HIST1H3B H3C3 H3FC HIST1H3C H3C4 HIST1H3D H3C6 HIST1H3E H3C7 HIST1H3F H3C8 HIST1H3G H3C10 HIST1H3H H3C11 HIST1H3I H3C12 HIST1H3J protein protein human Homo sapiens Human MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA ipl1_yeast Spindle assembly checkpoint kinase IPL1 YPL209C Aurora kinase Increase-in-ploidy protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MQRNSLVNIKLNANSPSKKTTTRPNTSRINKPWRISHSPQQRNPNSKIPSPVREKLNRLPVNNKKFLDMESSKIPSPIRKATSSKMIHENKKLPKFKSLSLDDFELGKKLGKGKFGKVYCVRHRSTGYICALKVMEKEEIIKYNLQKQFRREVEIQTSLNHPNLTKSYGYFHDEKRVYLLMEYLVNGEMYKLLRLHGPFNDILASDYIYQIANALDYMHKKNIIHRDIKPENILIGFNNVIKLTDFGWSIINPPENRRKTVCGTIDYLSPEMVESREYDHTIDAWALGVLAFELLTGAPPFEEEMKDTTYKRIAALDIKMPSNISQDAQDLILKLLKYDPKDRMRLGDVKMHPWILRNKPFWENKRL suvar39h1-p16105-1 Interaction detected by methylation reaction. 122850 N/A 122852 enzyme enzyme unspecified role unspecified role human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 N/A 122853 enzyme target enzyme target substrate substrate unspecified role unspecified role methylation methylation reaction methylation false false false SUVAR39H1 was myc-tagged. C terminal region of SUVAR39H1 is necessary for interaction (AA118-412). H3 is methylated. Reaction observed in the presence of SAM as methyl-group donor. suvar39h1-p16105-2 Interaction detected by methylation reaction. 122850 N/A 122854 enzyme enzyme unspecified role unspecified role ecoli Escherichia coli (strain K12) N/A 122853 enzyme target enzyme target substrate substrate unspecified role unspecified role methylation methylation reaction methylation false false false Methylation of histone H3. SAM necessary for reaction. Suvar39h1 (AA 82-412) was GST-tagged. C terminal region of Suv39h1 (AA 221-412)is necessary for interaction. suvar39h1-p16106-3 Interaction detected by methylation reaction. 122850 N/A 122852 enzyme enzyme unspecified role unspecified role human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 N/A 122853 enzyme target enzyme target substrate substrate unspecified role unspecified role methylation methylation reaction methylation false false false AA 82-412 of SUV39H1 was GST tagged. SAM required for reaction. H3 is methylated. clr4-p16106-1 Interaction detected by methylation reaction. 122850 N/A 122853 enzyme target enzyme target substrate substrate unspecified role unspecified role N/A 122855 enzyme enzyme unspecified role unspecified role ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false SAM necessary for the reaction. CLR4 GST tagged. Methylation of histone H3. suv39h2-p16106-1 interaction detected by methylation reaction. 122850 N/A 122853 enzyme target enzyme target substrate substrate unspecified role unspecified role N/A 122856 enzyme enzyme unspecified role unspecified role ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false SAM necessary for this reaction. Suv39h2 GST tagged. Methylation of histone H3. suv39h1-p16106-4 Interaction detected by methylation reaction. 122851 N/A 122854 enzyme enzyme unspecified role unspecified role ecoli Escherichia coli (strain K12) N/A 122857 enzyme target enzyme target substrate substrate unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) methylation methylation reaction methylation false false false AA 82-412 of Suvar39h1 was GST tagged. AA 1-20 of H3 was chemically synthesized. SAM was necessary as methyl group donor for reaction. Mehtylation of H3 peptide at K9 position. ipl1-p16106-1 Interaction detected by phosphorylation 122851 N/A 122858 enzyme enzyme unspecified role unspecified role ecoli Escherichia coli (strain K12) N/A 122857 enzyme target enzyme target substrate substrate unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) phosphorylation phosphorylation reaction phosphorylation false false false Aurora is His tagged. H3 peptide AA 1-20 phosphorylated at S10. Phosphorylation of H3 peptide at S10.