bhf-ucl liu-1997e-1 Dual role of the Smad4/DPC4 tumor suppressor in TGFbeta-inducible transcriptional complexes. vison-r1b/l17 Neovison vison lung epithelial cells (Mv1Lu) without TGFbeta type1 receptor Mustela vison lung_epithelium lung epithelial cells anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Liu F., Pouponnot C., Massagué J. Cardiac - Interactions involving cardiac related proteins Smad-4 is human (confirmed in Hata et al.1997. PMID: 9214507)) j-massague@ski.mskcc.org Genes Dev. (0890-9369) 1997 imex curation Species origin of FAST-1 (Xenopus laevis) was inferred from constructions in Fig. 3B. Only protein-protein interactions liu-1997e-2 Dual role of the Smad4/DPC4 tumor suppressor in TGFbeta-inducible transcriptional complexes. human-sw480 Human SW480 adenocarcinoma cell line sw480 SW480- human primary colorectal adenocarcinoma cell line There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution and a C -> T mutation in codon 309 resulting in a Pro -> Ser substitution. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CCL-228&Template=cellBiology anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Liu F., Pouponnot C., Massagué J. Cardiac - Interactions involving cardiac related proteins Smad-4 is human (confirmed in Hata et al.1997. PMID: 9214507)) j-massague@ski.mskcc.org Genes Dev. (0890-9369) 1997 imex curation Species origin of FAST-1 (Xenopus laevis) was inferred from constructions in Fig. 3B. Only protein-protein interactions liu-1997e-3 Dual role of the Smad4/DPC4 tumor suppressor in TGFbeta-inducible transcriptional complexes. chlae-cos Chlorocebus aethiops cos cos anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Liu F., Pouponnot C., Massagué J. Cardiac - Interactions involving cardiac related proteins Re-IP. Smad-4 is human (confirmed in Hata et al.1997. PMID: 9214507)) j-massague@ski.mskcc.org Genes Dev. (0890-9369) 1997 imex curation Species origin of FAST-1 (Xenopus laevis) was inferred from constructions in Fig. 3B. Only protein-protein interactions liu-1997e-4 Dual role of the Smad4/DPC4 tumor suppressor in TGFbeta-inducible transcriptional complexes. chlae-cos Chlorocebus aethiops cos cos anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Liu F., Pouponnot C., Massagué J. Cardiac - Interactions involving cardiac related proteins Smad-4 is human (confirmed in Hata et al.1997. PMID: 9214507)) j-massague@ski.mskcc.org Genes Dev. (0890-9369) 1997 imex curation Species origin of FAST-1 (Xenopus laevis) was inferred from constructions in Fig. 3B. Only protein-protein interactions smad2_human Mothers against decapentaplegic homolog 2 SMAD2 MADH2 MADR2 Mad-related protein 2 SMAD family member 2 JV18-1 protein protein human Homo sapiens Human MSSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTPNTIDQWDTTGLYSFSEQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPPPGYISEDGETSDQQLNQSMDTGSPAELSPTTLSPVNHSLDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS foxh1_xenla Forkhead box protein H1 foxh1 fast-1 fast1 foxh1a Forkhead activin signal transducer 1 XFoxH1a protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MRDPSSLYSGFPAGSQYESVEPPSLALLSSIDQEQLPVATGQSYNHSVQHWPQPWPPLSLYREGGTWSPDRGSMYGLSPGTHEGSCTHTHEGPKDSMAGDQTRSRKSKKKNYHRYNKPPYSYLAMIALVIQNSPEKRLKLSQILKEVSTLFPFFNGDYMGWKDSIRHNLSSSDCFKKILKDPGKPQAKGNFWTVDVSRIPLDAMKLQNTALTRGGSDYFVQDLAPYILHNYKYEHNAGAYGHQMPPSHARSLSLAEDSQQTNTGGKLNTSFMIDSLLHDLQEVDLPDASRNLENQRISPAVAMNNMWSSAPLLYTHSKPTRNARSPGLSTIHSTYSSSSSSISTISPVGFQKEQEKSGRQTQRVGHPIKRSREDDDCSTTSSDPDTGNYSPIEPPKKMPLLSLDLPTSYTKSVAPNVVAPPSVLPFFHFPRFTYYNYGPSPYMTPPYWGFPHPTNSGGDSPRGPQSPLDLDNMLRAMPPNKSVFDVLTSHPGDLVHPSFLSQCLGSSGSPYPSRQGLM smad4_human Mothers against decapentaplegic homolog 4 SMAD4 DPC4 MADH4 SMAD family member 4 Deletion target in pancreatic carcinoma 4 protein protein human Homo sapiens Human MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSSMMVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTANFPNIPVASTSQPASILGGSHSEGLLQIASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPHPGHYWPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD smad2-foxh1-1 1597318 n/a 1597322 unspecified role unspecified role bait bait Q15796:p.[Ser464Ala;Ser465Ala;Ser467Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q15796:p.Asp450Glu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1597323 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3A TGFbeta The isolated C domain of Smad2 (248-467aa) interacted constitutively, albeit weakly, with FAST-1, and this interaction was increased by TGFbeta addition. R1B/L17 cells were cotransfected with the indicated vectors and TbetaR-I for TGFbeta stimulation foxh1-smad2 1597318 n/a 1597323 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1597322 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3C TGFbeta R1B/L17 cells were cotransfected with the indicated vectors and TbetaR-I for TGFbeta stimulation. smad2-foxh1-2 1597319 n/a 1597323 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1597322 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 4A TGFbeta foxh1-smad4-1 1597319 n/a 1597324 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1597323 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 4B TGFbeta Smad 2 foxh1-smad2-4 1597320 n/a 1597323 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1597324 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1597322 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 4C TGFbeta Flag-Smad2 was the bait in the first immunoprecipitation. COS cells were cotransfected with the indicated vectors, and TbetaR-I(T204D) for TGF-beta stimulation smad2-smad4-2 1597321 n/a 1597324 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1597322 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fg. 4D TGFbeta Myc-FAST COS cells were cotransfected with the indicated vectors, and TbetaR-I(T204D) for TGFbeta stimulation