IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ahmad-2012-7 mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Ahmad Waza A., Andrabi K., Ul Hussain M. Cardiac - Interactions involving cardiac related proteins Kir6.1 has been entered as being of Rattine origin; blat of primers gives a match to both Mouse and Rat genomes (there is only 4 amino acid differences between the two proteins), but the pCMV Kir6.1 construct (gift by Prof. Susumu Seino) is only found in pmid:17001304 (Rat origin for Kir6.1) and a further publication -pmid:19959479, in which Kir6.1 DNA was also donated by Prof. Seino, uses Rat Kir6.1. Connexin 43 has been entered as being of Rattine origin; as given in reference 21 (HA tagged construct). hussainjnu@yahoo.com,mahboob@kashmiruniversity.ac.in Experimental cell research 2012 imex curation Kir6.1 has been entered as being of Rattine origin; blat of primers gives a match to both Mouse and Rat genomes (there is only 4 amino acid differences between the two proteins), but the pCMV Kir6.1 construct (gift by Prof. Susumu Seino) is only found in pmid:17001304 (Rat origin for Kir6.1) and a further publication -pmid:19959479, in which Kir6.1 DNA was also donated by Prof. Seino, uses Rat Kir6.1. Th CMV-HA-Connexin 43 construct has been entered as being of Rattine origin; as given in reference 21 (HA tagged construct). Only protein-protein interactions imex curation ahmad-2012-3 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Ahmad Waza A., Andrabi K., Ul Hussain M. Cardiac - Interactions involving cardiac related proteins Kir6.1 has been entered as being of Rattine origin; blat of primers gives a match to both Mouse and Rat genomes (there is only 4 amino acid differences between the two proteins), but the pCMV Kir6.1 construct (gift by Prof. Susumu Seino) is only found in pmid:17001304 (Rat origin for Kir6.1) and a further publication -pmid:19959479, in which Kir6.1 DNA was also donated by Prof. Seino, uses Rat Kir6.1. Connexin 43 has been entered as being of Rattine origin; as given in reference 21 (HA tagged construct). hussainjnu@yahoo.com,mahboob@kashmiruniversity.ac.in Experimental cell research 2012 imex curation Kir6.1 has been entered as being of Rattine origin; blat of primers gives a match to both Mouse and Rat genomes (there is only 4 amino acid differences between the two proteins), but the pCMV Kir6.1 construct (gift by Prof. Susumu Seino) is only found in pmid:17001304 (Rat origin for Kir6.1) and a further publication -pmid:19959479, in which Kir6.1 DNA was also donated by Prof. Seino, uses Rat Kir6.1. Th CMV-HA-Connexin 43 construct has been entered as being of Rattine origin; as given in reference 21 (HA tagged construct). Only protein-protein interactions imex curation ahmad-2012-4 mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Ahmad Waza A., Andrabi K., Ul Hussain M. Cardiac - Interactions involving cardiac related proteins Kir6.1 has been entered as being of Rattine origin; blat of primers gives a match to both Mouse and Rat genomes (there is only 4 amino acid differences between the two proteins), but the pCMV Kir6.1 construct (gift by Prof. Susumu Seino) is only found in pmid:17001304 (Rat origin for Kir6.1) and a further publication -pmid:19959479, in which Kir6.1 DNA was also donated by Prof. Seino, uses Rat Kir6.1. Connexin 43 has been entered as being of Rattine origin; as given in reference 21 (HA tagged construct). hussainjnu@yahoo.com,mahboob@kashmiruniversity.ac.in Experimental cell research 2012 imex curation Kir6.1 has been entered as being of Rattine origin; blat of primers gives a match to both Mouse and Rat genomes (there is only 4 amino acid differences between the two proteins), but the pCMV Kir6.1 construct (gift by Prof. Susumu Seino) is only found in pmid:17001304 (Rat origin for Kir6.1) and a further publication -pmid:19959479, in which Kir6.1 DNA was also donated by Prof. Seino, uses Rat Kir6.1. Th CMV-HA-Connexin 43 construct has been entered as being of Rattine origin; as given in reference 21 (HA tagged construct). Only protein-protein interactions imex curation ahmad-2012-5 mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ahmad Waza A., Andrabi K., Ul Hussain M. Cardiac - Interactions involving cardiac related proteins Kir6.1 has been entered as being of Rattine origin; blat of primers gives a match to both Mouse and Rat genomes (there is only 4 amino acid differences between the two proteins), but the pCMV Kir6.1 construct (gift by Prof. Susumu Seino) is only found in pmid:17001304 (Rat origin for Kir6.1) and a further publication -pmid:19959479, in which Kir6.1 DNA was also donated by Prof. Seino, uses Rat Kir6.1. Connexin 43 has been entered as being of Rattine origin; as given in reference 21 (HA tagged construct). hussainjnu@yahoo.com,mahboob@kashmiruniversity.ac.in Experimental cell research 2012 imex curation Kir6.1 has been entered as being of Rattine origin; blat of primers gives a match to both Mouse and Rat genomes (there is only 4 amino acid differences between the two proteins), but the pCMV Kir6.1 construct (gift by Prof. Susumu Seino) is only found in pmid:17001304 (Rat origin for Kir6.1) and a further publication -pmid:19959479, in which Kir6.1 DNA was also donated by Prof. Seino, uses Rat Kir6.1. Th CMV-HA-Connexin 43 construct has been entered as being of Rattine origin; as given in reference 21 (HA tagged construct). Only protein-protein interactions imex curation ahmad-2012-6 mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Ahmad Waza A., Andrabi K., Ul Hussain M. Cardiac - Interactions involving cardiac related proteins Kir6.1 has been entered as being of Rattine origin; blat of primers gives a match to both Mouse and Rat genomes (there is only 4 amino acid differences between the two proteins), but the pCMV Kir6.1 construct (gift by Prof. Susumu Seino) is only found in pmid:17001304 (Rat origin for Kir6.1) and a further publication -pmid:19959479, in which Kir6.1 DNA was also donated by Prof. Seino, uses Rat Kir6.1. Connexin 43 has been entered as being of Rattine origin; as given in reference 21 (HA tagged construct). hussainjnu@yahoo.com,mahboob@kashmiruniversity.ac.in Experimental cell research 2012 imex curation Kir6.1 has been entered as being of Rattine origin; blat of primers gives a match to both Mouse and Rat genomes (there is only 4 amino acid differences between the two proteins), but the pCMV Kir6.1 construct (gift by Prof. Susumu Seino) is only found in pmid:17001304 (Rat origin for Kir6.1) and a further publication -pmid:19959479, in which Kir6.1 DNA was also donated by Prof. Seino, uses Rat Kir6.1. Th CMV-HA-Connexin 43 construct has been entered as being of Rattine origin; as given in reference 21 (HA tagged construct). Only protein-protein interactions imex curation ahmad-2012-8 rat-h9c2 h9c2 rat myoblasts h9c2 h9c2 rat myoblasts anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Ahmad Waza A., Andrabi K., Ul Hussain M. Cardiac - Interactions involving cardiac related proteins Kir6.1 has been entered as being of Rattine origin; blat of primers gives a match to both Mouse and Rat genomes (there is only 4 amino acid differences between the two proteins), but the pCMV Kir6.1 construct (gift by Prof. Susumu Seino) is only found in pmid:17001304 (Rat origin for Kir6.1) and a further publication -pmid:19959479, in which Kir6.1 DNA was also donated by Prof. Seino, uses Rat Kir6.1. Connexin 43 has been entered as being of Rattine origin; as given in reference 21 (HA tagged construct). hussainjnu@yahoo.com,mahboob@kashmiruniversity.ac.in Experimental cell research 2012 imex curation Kir6.1 has been entered as being of Rattine origin; blat of primers gives a match to both Mouse and Rat genomes (there is only 4 amino acid differences between the two proteins), but the pCMV Kir6.1 construct (gift by Prof. Susumu Seino) is only found in pmid:17001304 (Rat origin for Kir6.1) and a further publication -pmid:19959479, in which Kir6.1 DNA was also donated by Prof. Seino, uses Rat Kir6.1. Th CMV-HA-Connexin 43 construct has been entered as being of Rattine origin; as given in reference 21 (HA tagged construct). Only protein-protein interactions imex curation ahmad-2012-9 rat-h9c2 h9c2 rat myoblasts h9c2 h9c2 rat myoblasts anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Ahmad Waza A., Andrabi K., Ul Hussain M. Cardiac - Interactions involving cardiac related proteins Kir6.1 has been entered as being of Rattine origin; blat of primers gives a match to both Mouse and Rat genomes (there is only 4 amino acid differences between the two proteins), but the pCMV Kir6.1 construct (gift by Prof. Susumu Seino) is only found in pmid:17001304 (Rat origin for Kir6.1) and a further publication -pmid:19959479, in which Kir6.1 DNA was also donated by Prof. Seino, uses Rat Kir6.1. Th CMV-HA-Connexin 43 construct has been entered as being of Rattine origin; as given in reference 21 (HA tagged construct). hussainjnu@yahoo.com,mahboob@kashmiruniversity.ac.in Experimental cell research 2012 Only protein-protein interactions imex curation imex curation kcnj8_rat ATP-sensitive inward rectifier potassium channel 8 Kcnj8 Inward rectifier K(+) channel Kir6.1 Potassium channel, inwardly rectifying subfamily J member 8 uKATP-1 protein protein rat Rattus norvegicus (Rat) Rat MLARKSIIPEEYVLARIAAENLRKPRIRDRLPKARFIAKSGACNLAHKNIREQGRFLQDIFTTLVDLKWRHTLVIFTMSFLCSWLLFAIMWWLVAFAHGDIYAYMEKGITEKSGLESAVCVTNVRSFTSAFLFSIEVQVTIGFGGRMMTEECPLAITVLILQNIVGLIINAVMLGCIFMKTAQAHRRAETLIFSRHAVIAVRNGKLCFMFRVGDLRKSMIISASVRIQVVKKTTTPEGEVVPIHQQDIPVDNPIESNNIFLVAPLIICHVIDKRSPLYDISATDLVNQDLEVIVILEGVVETTGITTQARTSYIAEEIQWGHRFVSIVTEEEGVYSVDYSKFGNTVRVAAPRCSARELDEKPSILIQTLQKSELSHQNSLRKRNSMRRNNSMRRSNSIRRNNSSLMVPKVQFMTPEGNQCPSES mint cxa1_rat Gap junction alpha-1 protein Gja1 Cxn-43 Connexin-43 Gap junction 43 kDa heart protein protein protein rat Rattus norvegicus (Rat) Rat MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVEMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSVFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGRSDPYHATTGPLSPSKDCGSPKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNAKKVAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI cxa1_mouse Gap junction alpha-1 protein Gja1 Cxn-43 Connexin-43 Gap junction 43 kDa heart protein protein protein mouse Mus musculus Mouse MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVEMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSVFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGRSDPYHATTGPLSPSKDCGSPKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDSQNAKKVAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI gja1-kcnj8-2 1792301 N/A 1792308 unspecified role unspecified role bait bait n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1792309 unspecified role unspecified role prey prey n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P08050:p.Ser262Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3B kcnj8-gja1-4 1792301 N/A 1792308 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1792309 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P08050:p.Ser262Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3B-2 kcnj8-gja1-1 1792302 N/A 1792308 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 1792310 unspecified role unspecified role prey prey mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells physical association physical association false false false 1A kcnj8-gja1-2 1792303 N/A 1792308 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1792310 unspecified role unspecified role bait bait physical association physical association false false false 1B gja1-kcnj8-1 1792304 N/A 1792308 unspecified role unspecified role bait bait n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1792310 unspecified role unspecified role prey prey N/A opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1C, 4B potato acid phosphatase;very little phospho-S262-Cx43 protein was co-immunoprecipitated from the phosphatase treated cell extract kcnj8-gja1-3 1792305 N/A 1792308 unspecified role unspecified role neutral component neutral component n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1792310 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 2 gja1-kcnj8-3 1792306 N/A 1792308 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1792309 unspecified role unspecified role bait bait physical association physical association false false false Supp 1A gja1-kcnj8-4 1792307 N/A 1792308 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1792309 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P08050:p.Ser262Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Supp 1B