bhf-ucl basu-2008-2 The major chemical-detoxifying system of UDP-glucuronosyltransferases requires regulated phosphorylation supported by protein kinase C. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Basu N.K., Kole L., Basu M., Chakraborty K., Mitra P.S., Owens I.S. Cardiac - Interactions involving cardiac related proteins UGT1A7 and UGT1A10 were human genes. Confirmed in Basu N. K. et al. 2004 (PMID: 14557274) owensi@mail.nih.gov J. Biol. Chem. (0021-9258) 2008 imex curation Only protein-protein interactions basu-2008-1 The major chemical-detoxifying system of UDP-glucuronosyltransferases requires regulated phosphorylation supported by protein kinase C. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Basu N.K., Kole L., Basu M., Chakraborty K., Mitra P.S., Owens I.S. Cardiac - Interactions involving cardiac related proteins UGT1A7 and UGT1A10 were human genes. Confirmed in Basu N. K. et al. 2004 (PMID: 14557274) owensi@mail.nih.gov J. Biol. Chem. (0021-9258) 2008 imex curation Only protein-protein interactions basu-2008-3 The major chemical-detoxifying system of UDP-glucuronosyltransferases requires regulated phosphorylation supported by protein kinase C. in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Basu N.K., Kole L., Basu M., Chakraborty K., Mitra P.S., Owens I.S. Cardiac - Interactions involving cardiac related proteins UGT1A7 and UGT1A10 were human genes. Confirmed in Basu N. K. et al. 2004 (PMID: 14557274) owensi@mail.nih.gov J. Biol. Chem. (0021-9258) 2008 imex curation Only protein-protein interactions ud110_human UDP-glucuronosyltransferase 1A10 UDP-glucuronosyltransferase 1-10 UGT1A10 GNT1 UGT1 UDP-glucuronosyltransferase 1-J protein protein human Homo sapiens Human MARAGWTSPVPLCVCLLLTCGFAEAGKLLVVPMDGSHWFTMQSVVEKLILRGHEVVVVMPEVSWQLERSLNCTVKTYSTSYTLEDQNREFMVFAHAQWKAQAQSIFSLLMSSSSGFLDLFFSHCRSLFNDRKLVEYLKESSFDAVFLDPFDTCGLIVAKYFSLPSVVFTRGIFCHHLEEGAQCPAPLSYVPNDLLGFSDAMTFKERVWNHIVHLEDHLFCQYLFRNALEIASEILQTPVTAYDLYSHTSIWLLRTDFVLDYPKPVMPNMIFIGGINCHQGKPLPMEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVLWRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPLFGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRPVEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKCCAYGYRKCLGKKGRVKKAHKSKTH pkcalpha_chlae_protein PKalpha chlae protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey similar to UniProt P17252 pkcdelta_chlae_protein PKCdelta chlae protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey similar to UniProt Q05655 ud17_human UDP-glucuronosyltransferase 1A7 UDP-glucuronosyltransferase 1-7 UGT1A7 GNT1 UGT1 UDP-glucuronosyltransferase 1-G protein protein human Homo sapiens Human MARAGWTGLLPLYVCLLLTCGFAKAGKLLVVPMDGSHWFTMQSVVEKLILRGHEVVVVMPEVSWQLGRSLNCTVKTYSTSYTLEDQDREFMVFADARWTAPLRSAFSLLTSSSNGIFDLFFSNCRSLFNDRKLVEYLKESCFDAVFLDPFDACGLIVAKYFSLPSVVFARGIFCHYLEEGAQCPAPLSYVPRLLLGFSDAMTFKERVWNHIMHLEEHLFCPYFFKNVLEIASEILQTPVTAYDLYSHTSIWLLRTDFVLEYPKPVMPNMIFIGGINCHQGKPVPMEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVLWRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPLFGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRPVEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKCCAYGYRKCLGKKGRVKKAHKSKTH pkcepsilon_chlae_protein PKCepsilon chlae protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Similar to UniProt Q02156 epsilon-rack_chlae_protein epsilon-RACK chale protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey similar to UniProt P63244 kpce_human Protein kinase C epsilon type nPKC-epsilon PRKCE PKCE protein protein human Homo sapiens Human MVVFNGLLKIKICEAVSLKPTAWSLRHAVGPRPQTFLLDPYIALNVDDSRIGQTATKQKTNSPAWHDEFVTDVCNGRKIELAVFHDAPIGYDDFVANCTIQFEELLQNGSRHFEDWIDLEPEGRVYVIIDLSGSSGEAPKDNEERVFRERMRPRKRQGAVRRRVHQVNGHKFMATYLRQPTYCSHCRDFIWGVIGKQGYQCQVCTCVVHKRCHELIITKCAGLKKQETPDQVGSQRFSVNMPHKFGIHNYKVPTFCDHCGSLLWGLLRQGLQCKVCKMNVHRRCETNVAPNCGVDARGIAKVLADLGVTPDKITNSGQRRKKLIAGAESPQPASGSSPSEEDRSKSAPTSPCDQEIKELENNIRKALSFDNRGEEHRAASSPDGQLMSPGENGEVRQGQAKRLGLDEFNFIKVLGKGSFGKVMLAELKGKDEVYAVKVLKKDVILQDDDVDCTMTEKRILALARKHPYLTQLYCCFQTKDRLFFVMEYVNGGDLMFQIQRSRKFDEPRSRFYAAEVTSALMFLHQHGVIYRDLKLDNILLDAEGHCKLADFGMCKEGILNGVTTTTFCGTPDYIAPEILQELEYGPSVDWWALGVLMYEMMAGQPPFEADNEDDLFESILHDDVLYPVWLSKEAVSILKAFMTKNPHKRLGCVASQNGEDAIKQHPFFKEIDWVLLEQKKIKPPFKPRIKTKRDVNNFDQDFTREEPVLTLVDEAIVKQINQEEFKGFSYFGEDLMP pkcalpha-ugt1a10-1 1539257 n/a 1539260 unspecified role unspecified role neutral component neutral component n/a 1539261 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 4C pkcdelta-ugt1a10-1 1539257 n/a 1539260 unspecified role unspecified role neutral component neutral component c-terminus his L-histidine residue (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 2-azanyl-3-(1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 4-(2-amino-2-carboxyethyl)imidazole L-histidine alpha-amino-beta-(4-imidazole)propionic acid glyoxaline-5-alanine His c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1539262 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 4D pkcepsilon-ugt1a7-1 1539257 n/a 1539263 unspecified role unspecified role neutral component neutral component c-terminus his L-histidine residue (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 2-azanyl-3-(1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 4-(2-amino-2-carboxyethyl)imidazole L-histidine alpha-amino-beta-(4-imidazole)propionic acid glyoxaline-5-alanine His c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1539264 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 3SA ugt1a7-epsilon_rack-1 1539258 n/a 1539263 unspecified role unspecified role bait bait c-terminus his L-histidine residue (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 2-azanyl-3-(1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 4-(2-amino-2-carboxyethyl)imidazole L-histidine alpha-amino-beta-(4-imidazole)propionic acid glyoxaline-5-alanine His c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1539264 unspecified role unspecified role prey prey n/a 1539265 unspecified role unspecified role prey prey physical association physical association false false false Fig. 5B Curcumin Calphostin ugt1a10-pkcalpha-1 1539258 n/a 1539260 unspecified role unspecified role bait bait c-terminal his L-histidine residue (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 2-azanyl-3-(1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 4-(2-amino-2-carboxyethyl)imidazole L-histidine alpha-amino-beta-(4-imidazole)propionic acid glyoxaline-5-alanine His c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1539261 unspecified role unspecified role prey prey n/a 1539262 unspecified role unspecified role prey prey physical association physical association false false false Fig. 5C Curcumin inhibits binding of UGT1A10 to PKC delta Calphostin inhibits binding of UGT1A10 to PKC delta prkce-ugt1a7 1539259 n/a 1539263 enzyme target enzyme target substrate substrate unspecified role unspecified role c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1539266 enzyme enzyme unspecified role unspecified role phosphorylation phosphorylation reaction phosphorylation false false false Fig. 6E Q02156 increases activity of Q9HAW7 Q9HAW7 phosphorylated residue