bhf-ucl li-2005j-1 Polycystin 2 interacts with type I inositol 1,4,5-trisphosphate receptor to modulate intracellular Ca2+ signaling. xenopus laevis laevis-oocyte Xenopus laevis laevis oocyte Oocyte anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Li Y., Wright J.M., Qian F., Germino G.G., Guggino W.B. Cardiac - Interactions involving cardiac related proteins They use Xenopus oocytes. Confirmed in ref. 33 (PMID:14699087) PC2 is human and myc tag is n-terminal. Confirmed in ref. 15 (PMID: 11140688) wguggino@jhmi.edu J. Biol. Chem. (0021-9258) 2005 imex curation Only protein-protein interactions li-2005j-2 Polycystin 2 interacts with type I inositol 1,4,5-trisphosphate receptor to modulate intracellular Ca2+ signaling. xenopus laevis laevis-oocyte Xenopus laevis laevis oocyte Oocyte anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Li Y., Wright J.M., Qian F., Germino G.G., Guggino W.B. Cardiac - Interactions involving cardiac related proteins They use Xenopus oocytes. Confirmed in ref. 33 (PMID:14699087) PC2 is human and myc tag is n-terminal. Confirmed in ref. 15 (PMID: 11140688) wguggino@jhmi.edu J. Biol. Chem. (0021-9258) 2005 imex curation Only protein-protein interactions pkd2_human Polycystin-2 PKD2 Polycystic kidney disease 2 protein Autosomal dominant polycystic kidney disease type II protein Polycystwin R48321 TRPP2 Transient receptor potential cation channel subfamily P member 2 protein protein human Homo sapiens Human MVNSSRVQPQQPGDAKRPPAPRAPDPGRLMAGCAAVGASLAAPGGLCEQRGLEIEMQRIRQAAARDPPAGAAASPSPPLSSCSRQAWSRDNPGFEAEEEEEEVEGEEGGMVVEMDVEWRPGSRRSAASSAVSSVGARSRGLGGYHGAGHPSGRRRRREDQGPPCPSPVGGGDPLHRHLPLEGQPPRVAWAERLVRGLRGLWGTRLMEESSTNREKYLKSVLRELVTYLLFLIVLCILTYGMMSSNVYYYTRMMSQLFLDTPVSKTEKTNFKTLSSMEDFWKFTEGSLLDGLYWKMQPSNQTEADNRSFIFYENLLLGVPRIRQLRVRNGSCSIPQDLRDEIKECYDVYSVSSEDRAPFGPRNGTAWIYTSEKDLNGSSHWGIIATYSGAGYYLDLSRTREETAAQVASLKKNVWLDRGTRATFIDFSVYNANINLFCVVRLLVEFPATGGVIPSWQFQPLKLIRYVTTFDFFLAACEIIFCFFIFYYVVEEILEIRIHKLHYFRSFWNCLDVVIVVLSVVAIGINIYRTSNVEVLLQFLEDQNTFPNFEHLAYWQIQFNNIAAVTVFFVWIKLFKFINFNRTMSQLSTTMSRCAKDLFGFAIMFFIIFLAYAQLAYLVFGTQVDDFSTFQECIFTQFRIILGDINFAEIEEANRVLGPIYFTTFVFFMFFILLNMFLAIINDTYSEVKSDLAQQKAEMELSDLIRKGYHKALVKLKLKKNTVDDISESLRQGGGKLNFDELRQDLKGKGHTDAEIEAIFTKYDQDGDQELTEHEHQQMRDDLEKEREDLDLDHSSLPRPMSSRSFPRSLDDSEEDDDEDSGHSSRRRGSISSGVSYEEFQVLVRRVDRMEHSIGSIVSKIDAVIVKLEIMERAKLKRREVLGRLLDGVAEDERLGRDSEIHREQMERLVREELERWESDDAASQISHGLGTPVGLNGQPRPRSSRPSSSQSTEGMEGAGGNGSSNVHV Functions as a calcium permeable cation channel. PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis Defects in PKD2 are the cause of polycystic kidney disease autosomal dominant type 2 (ADPKD2) [MIM:173900]. ADPKD2 represents approximately 15% of the cases of ADPKD, a common genetic disease affecting about 1:400 to 1:1000 individuals. ADPKD is characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. ADPKD2 is clinically milder than ADPKD1 but it has a deleterious impact on overall life expectancy mint q91908_xenla Inositol 1,4,5-trisphosphate receptor itpr1.S itpr1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSDKMSSFLHVGDIVSLYAEGSVNGFINTLGLVDDRSVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGASSTTDTVLLNKLHHAADLEKKQNETENRKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDEAGNEGSWFYIQPFYKLRSIGDSVVIGDKVVLNPVNAGQPLHASSQQLSDNPGCNEVNSVNCNTSWKIVLFMKWSDNKDDILKGGDVVRLFHAEQEKFLTCDEHRKKQHVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEIDAEPDAGNARMQVVPEKMMCTLISVPEGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNSPIDKDEEKPVMLRIGTSPVKEDKEAFAIVPVSPAEVRDLDFANDASKVLSSIAGKLEKGTITQNERRAVTKLLEELVYFVTGLVNSGHDILEVLVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELGDQRHAPFRHICRLCYRVLRHSQQDYRKNQEYIAKQFGFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICKAVLNPANADILIETKLVLSRFEFEGVASGESSLEAGEDEEEVWLFWKASNKEIRSRSVRELAQDAKEGQKEDQDVLNYYRYQLNLFARMCLDRQYLAINEISGQLDVDLILRCMSDENLPFDLRASFCRLMLHMHVDRDPQEQVTPVKYARLWSEIPSEIAIDDYDSSGTSRDDIKERFAQTMEFVEEYLRDVVGQRFPFADKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAIVDCVHLIANFPSGKLGKGEESKGGSNVMRSIHGVGELMTQVVLRGGGFLPMTPMAAPEGTIKAQREPEKEDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKSEFDESNAQSVEGSTEAITVVPGTLDFEHIEEQAEGIFGGSEENTPLDLDDDGGRTFLRVLLHLTMHDYPPLVSGALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVDNYKQIKQDLDQLRSIVEKSELWVYKGSGPEEVTAAQAGGADKGETPGKAKKSESTSSYNYRVVKEILLRLSKLCVQENTTGRRNRKQQQRLLRNMGAHSVVLELLQIPYEKTEDTRMQEIMKIAHEFLQNFCAGNQQNQALLHKHINLFLTPGILEAVTMQHIFMNNFQLCSEINERVVQHFAHCIETHGRNVQYIKFLQTIVKAEGRYIKKCQDIVMAELVNSGEDVLVFYNDRASFQTLVQMMRSERERMDENSPLMYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCVPEVKIAYINFLNHCYVDTEVEMKEIYTSNHMWKLFENFLVDICRVCNSTSDRKHADIVLERYVTEIVMSIVNTFFSSPFSDQSTTLQASKLAVVHQTRQPVFVQLLQAIFRVYHCNWLLPSQKASVESCIRVLSDVAKSRTIAIPVDLDSQVNNLFLKSHNIVQKTAMSWRMSVRNAARRDSVLTASRDYRNIIERLQDIVSSLEDRLRPLVQAELSVLVDVLHRPELLFPENTDARKKCESGQFICKLIRHTKLLLEENEEKLCIKVLQTLREMMAKDRGFGDKGEALRVILVNRYYGNIRPAGRRESLTSFGNGPLSGGSSGKSGGGSIGSGSLSRSEMSLSDVQCHLDKQGASDLVIDLIMNATSDRVFHESILLAIALLEGGNTTIQHSFFCRLTEDKKSEKFFKVFFDRMKVAQQEIKATVTVNTSDLGSKKRDEEQSERETPHHQRVREPSGQITEEAKEQLIEASVATKKAYYSFRREADPDDHFSLGEGVMAVAEKGRDELEMSAVITIMQPILRFLQLLCENHNRDLQNFLRCQNNKSNYNLVCETLQFLDCICGSTTGGLGLLGLYINEKNVALINQTLESLTEYCQGPCHENQNCIATHESNGIDIITALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVIKKAYLQGEVEFEDGESGEDYAASPRNVGHNIYILAHQLARHNKELQHMLKPGVQTGEGDEALEFYAKHTAQIEIVRLDRTMEQIVFPVPSICEFLTKESKLRIYYTTERDEQGSKIHDFFQRSEDLFNEMNWQKKLRAQQVLYWCSRNMTFWSSISFNLAVLMNLLVAFFYPFVNVHGGTLDSRLSGLLWTAMLVSLAIVIVLPKPHGIRALIASTILRLIFSIGLQPTLFLLGAFNVCNKIIFLTSFVGNRGTFTRGYGAMVLDVEFLYHLLYLLICAMGVFVHEFFYSLLLFDLVYREETLLNVIKSVTRNGRSIILTAVLALILVYLFSIVGYLFFKDDFILEVDRLQNETIFPENGGTMTSELLYPEVCRVGSDTNCTHEELAQVTEEEEEEKEHTCETLLMCIVTVLSHGLRSGGGVGDVLRKPSKEEPLFAARVIYDLLFFFMVIIIVLNLIFGVIIDTFADLRSEKQKKEEVLKTTCFICGLERDKFDNKTATFEEHFKEEHNMWHYLFFIVLVKVKDSTEYTGPESYVAEMIKDRNLDWFPRMRAMSLVSSDSEGEQNELRNLQEKLESTMRLVTNLSGQLSELKDQMTEQRKQKQRIGLLGQPAHLNINPQQPA xenopus-pkd2 1548983 n/a 1548985 unspecified role unspecified role prey prey n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1548986 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4A, 5A, C pkd2-xenopus-2 1548984 n/a 1548985 unspecified role unspecified role bait bait n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1548986 unspecified role unspecified role prey prey physical association physical association false false false Fig. 4A