bhf-ucl furukawa-1992-1 Interaction of rat lecithin-cholesterol acyltransferase with rat apolipoprotein A-I and with lecithin-cholesterol vesicles. in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom predetermined predetermined participant predetermined Furukawa Y., Urano T., Hida Y., Itoh H., Takahashi C., Kimura S. Cardiac - Interactions involving cardiac related proteins shuichi.kimura@jt.com J. Biochem. (0021-924X) 1992 Only protein-protein interactions imex curation lcat_human Phosphatidylcholine-sterol acyltransferase LCAT Lecithin-cholesterol acyltransferase Phospholipid-cholesterol acyltransferase 1-alkyl-2-acetylglycerophosphocholine esterase Platelet-activating factor acetylhydrolase protein protein human Homo sapiens Human MGPPGSPWQWVTLLLGLLLPPAAPFWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTIWLDLNMFLPLGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHSLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLKEEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDLLAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQGRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPE apoa1_human Apolipoprotein A-I APOA1 Apolipoprotein A1 protein protein human Homo sapiens Human MKAAVLTLAVLFLTGSQARHFWQQDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQ apoa1_rat Apolipoprotein A-I Apoa1 Apolipoprotein A1 protein protein rat Rattus norvegicus (Rat) Rat MKAAVLAVALVFLTGCQAWEFWQQDEPQSQWDRVKDFATVYVDAVKDSGRDYVSQFESSTLGKQLNLNLLDNWDTLGSTVGRLQEQLGPVTQEFWANLEKETDWLRNEMNKDLENVKQKMQPHLDEFQEKWNEEVEAYRQKLEPLGTELHKNAKEMQRHLKVVAEEFRDRMRVNADALRAKFGLYSDQMRENLAQRLTEIKNHPTLIEYHTKASDHLKTLGEKAKPALDDLGQGLMPVLEAWKAKIMSMIDEAKKKLNA lcat_rat Phosphatidylcholine-sterol acyltransferase Lcat Lecithin-cholesterol acyltransferase Phospholipid-cholesterol acyltransferase 1-alkyl-2-acetylglycerophosphocholine esterase Platelet-activating factor acetylhydrolase protein protein rat Rattus norvegicus (Rat) Rat MGLPGSPWQWVLLLLGLLLPPATSFWLLNVLFPPHTTPKAELSNHTRPVILVPGCMGNRLEAKLDKPNVVNWLCYRKTEDFFTIWLDFNMFLPLGVDCWIDNTRVVYNRSSGHMSNAPGVQIRVPGFGKTYSVEYLDDNKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLAPRQQDEYYQKLAGLVEEMYAAYGKPVFLIGHSLGCLHVLHFLLRQPQSWKDHFIDGFISLGAPWGGSIKPMRILASGDNQGIPIMSNIKLREEQRITTTSPWMFPAHHVWPEDHVFISTPNFNYTGQDFERFFADLHFEEGWHMFLQSRDLLAGLPAPGVEVYCLYGVGMPTAHTYIYDHNFPYKDPVAALYEDGDDTVATRSTELCGQWQGRQSQAVHLLPMNGTDHLNMVFSNKTLEHINAILLGAYRHGTPKSPTASLGPPTKE apoa1-lcat-1 1479750 n/a 1479751 unspecified role unspecified role prey prey human-plasma homo sapiens blood plasma plasma blood plasma n/a 1479752 unspecified role unspecified role bait bait human Homo sapiens Human direct interaction direct interaction false false false Fig. 3 A, B Human apoA1 was purified from human HDL apoa1-lcat-3 1479750 n/a 1479753 unspecified role unspecified role bait bait rat Rattus norvegicus (Rat) Rat n/a 1479754 unspecified role unspecified role prey prey rat-plasma RAT-plasma plasma blood plasma direct interaction direct interaction false false false Fig 3 C, D Rat apoAI was purified from rat HDL . Rat lcat was purified from rat plasma.