bhf-ucl meyers-2005-1 in vitro In vitro facs fluorescence-activated cell sorting FACS Flow cytometry facs antibody detection identification by antibody antibody detection Meyers JH., Chakravarti S., Schlesinger D., Illes Z., Waldner H., Umetsu SE., Kenny J., Zheng XX., Umetsu DT., DeKruyff RH., Strom TB., Kuchroo VK. Cardiac - Interactions involving cardiac related proteins The authors have confirmed that TIM-4 and TIM-1 constructs are of Murine origin. vkuchroo@rics.bwh.harvard.edu Nature immunology 2005 Only protein-protein interactions imex curation imex curation timd4_mouse T-cell immunoglobulin and mucin domain-containing protein 4 Timd4 Tim4 Spleen, mucin-containing, knockout of lymphotoxin protein T-cell immunoglobulin mucin receptor 4 T-cell membrane protein 4 protein protein mouse Mus musculus Mouse MSKGLLLLWLVTELWWLYLTPAASEDTIIGFLGQPVTLPCHYLSWSQSRNSMCWGKGSCPNSKCNAELLRTDGTRIISRKSTKYTLLGKVQFGEVSLTISNTNRGDSGVYCCRIEVPGWFNDVKKNVRLELRRATTTKKPTTTTRPTTTPYVTTTTPELLPTTVMTTSVLPTTTPPQTLATTAFSTAVTTCPSTTPGSFSQETTKGSAFTTESETLPASNHSQRSMMTISTDIAVLRPTGSNPGILPSTSQLTTQKTTLTTSESLQKTTKSHQINSRQTILIIACCVGFVLMVLLFLAFLLRGKVTGANCLQRHKRPDNTEDSDSVLNDMSHGRDDEDGIFTL q5qns5-2 Hepatitis A virus cellular receptor 1 homolog Kidney injury molecule 1 T cell immunoglobulin and mucin domain-containing protein 1 T cell membrane protein 1 T-cell immunoglobulin mucin receptor 1 Havcr1 Kim1 Tim1 Timd1 protein protein mouse Mus musculus Mouse MNQIQVFISGLILLLPGAVDSYVEVKGVVGHPVTLPCTYSTYRGITTTCWGRGQCPSSACQNTLIWTNGHRVTYQKSSRYNLKGHISEGDVSLTIENSVESDSGLYCCRVEIPGWFNDQKVTFSLQVKPEIPTRPPTRPTTTRPTATGRPTTISTRSTHVPTSIRVSTSTPPTSTHTWTHKPDWNGTVTSSGDTWSNHTEAIPPGKPQKNPTKGFYVGICIAALLLLLLVSTVAITRYILMKRKSASLSVVAFRVSKIEALQNAAVVHSRAEDNIYIVEDRP havr1_mouse Hepatitis A virus cellular receptor 1 homolog Kidney injury molecule 1 T cell immunoglobulin and mucin domain-containing protein 1 T cell membrane protein 1 T-cell immunoglobulin mucin receptor 1 Havcr1 Kim1 Tim1 Timd1 protein protein mouse Mus musculus Mouse MNQIQVFISGLILLLPGAVDSYVEVKGVVGHPVTLPCTYSTYRGITTTCWGRGQCPSSACQNTLIWTNGHRVTYQKSSRYNLKGHISEGDVSLTIENSVESDSGLYCCRVEIPGWFNDQKVTFSLQVKPEIPTRPPTRPTTTRPTATGRPTTISTRSTHVPTSIRVSTSTPPTSTHTWTHKPEPTTFCPHETTAEVTGIPSHTPTDWNGTVTSSGDTWSNHTEAIPPGKPQKNPTKGFYVGICIAALLLLLLVSTVAITRYILMKRKSASLSVVAFRVSKIEALQNAAVVHSRAEDNIYIVEDRP timd4-havcr1-1 3355062 N/A 3355063 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed ha tag HA tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c_terminal_pdgfr_tm_domain tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 3355064 unspecified role unspecified role prey prey purified purified region Timd4 binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c_terminus human IgG1 Fc tail fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association false false false Figure 3a second row far right The authors state that:-"There was staining of TIM-4 transfectants with TIM-1–Ig fusion protein whether full-length TIM-1–Ig, containing the TIM-1 IgV and mucin domains, or a TIM-1–Ig containing only the IgV domain was used". timd4-havcr1-2 3355062 N/A 3355063 unspecified role unspecified role prey prey purified purified c_terminus human IgG1 Fc tail fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd Havcr1 binding domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 3355065 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association false false false Figure 3a fourth row far left timd4-havcr1-3 3355062 N/A 3355063 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed ha tag HA tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c_terminal_pdgfr_tm_domain tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 3355064 unspecified role unspecified role prey prey purified purified region Timd4 binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c_terminus human IgG1 Fc tail fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association false false false Figure 3b havcr1-timd4-4 3355062 N/A 3355063 unspecified role unspecified role prey prey purified purified c_terminus human IgG1 Fc tail fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd Havcr1 binding domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 3355065 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association false false false Figure 3b havcr1-timd4-1 3355062 N/A 3355063 unspecified role unspecified role prey prey purified purified c_terminus human IgG1 Fc tail fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd Havcr1 binding domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 3355065 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level mouse-t-cell Mouse T cell t-cell Peripheral blood T-lymphocytes physical association physical association false false false Figure 4A TIM-4–Ig stained T cells activated with anti-CD3 and anti-CD28, but did not stain unactivated T cells. havcr1-timd4-5 3355062 N/A 3355063 unspecified role unspecified role prey prey purified purified c_terminus human IgG1 Fc tail fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd Havcr1 binding domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 3355065 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level mouse-spleen Mus musculus spleen spleen spleen physical association physical association false false false Figure 4B splenocytes were stimulated with concanavalin A.