bhf-ucl bared-2004-1 Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. human-macrophage Human macrophages macrophage Macrophage anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bared S.M., Buechler C., Boettcher A., Dayoub R., Sigruener A., Grandl M., Rudolph C., Dada A., Schmitz G. Cardiac - Interactions involving cardiac related proteins They use human monocytes derived macrophages gerd.schmitz@klinik.uni- regensburg.de gerd.schmitz@klinik.uniregensburg.de Mol. Biol. Cell (1059-1524) 2004 Only protein-protein interactions imex curation bared-2004-2 Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. human-e-ldl loaded macrophages Human-E-LDL loaded macrophages human-e-ldl loaded macrophages Human-E-LDL loaded macrophages anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bared S.M., Buechler C., Boettcher A., Dayoub R., Sigruener A., Grandl M., Rudolph C., Dada A., Schmitz G. Cardiac - Interactions involving cardiac related proteins Macrophages were incubated with enzymatically modified low-density lipoprotein (E-LDL) gerd.schmitz@klinik.uni- regensburg.de gerd.schmitz@klinik.uniregensburg.de Mol. Biol. Cell (1059-1524) 2004 Only protein-protein interactions imex curation bared-2004-3 Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. human-macrophage Human macrophages macrophage Macrophage fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Bared S.M., Buechler C., Boettcher A., Dayoub R., Sigruener A., Grandl M., Rudolph C., Dada A., Schmitz G. Cardiac - Interactions involving cardiac related proteins gerd.schmitz@klinik.uni- regensburg.de gerd.schmitz@klinik.uniregensburg.de Mol. Biol. Cell (1059-1524) 2004 Only protein-protein interactions imex curation bared-2004-4 Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. human-hl_60 Homo sapiens acute myeloid leukemia cell line hl_60 human acute myeloid leukemia cell line fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Bared S.M., Buechler C., Boettcher A., Dayoub R., Sigruener A., Grandl M., Rudolph C., Dada A., Schmitz G. Cardiac - Interactions involving cardiac related proteins The HL-60 cells were treated with PMA (phorbol 12-myristate 13-acetate) to induce macrophage differentiation gerd.schmitz@klinik.uni- regensburg.de gerd.schmitz@klinik.uniregensburg.de Mol. Biol. Cell (1059-1524) 2004 Only protein-protein interactions imex curation bared-2004-6 Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. human-e-ldl loaded macrophages Human-E-LDL loaded macrophages human-e-ldl loaded macrophages Human-E-LDL loaded macrophages density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Bared S.M., Buechler C., Boettcher A., Dayoub R., Sigruener A., Grandl M., Rudolph C., Dada A., Schmitz G. Cardiac - Interactions involving cardiac related proteins Human E-LDL loaded macrophages were solubilized in 1% Lubrol WX or 1% Triton X100 and fractionated by sucrose gradient centrifugation. gerd.schmitz@klinik.uni- regensburg.de gerd.schmitz@klinik.uniregensburg.de Mol. Biol. Cell (1059-1524) 2004 Only protein-protein interactions imex curation abca1_human Phospholipid-transporting ATPase ABCA1 ATP-binding cassette transporter 1 Cholesterol efflux regulatory protein ABCA1 ABC1 CERP ATP-binding cassette sub-family A member 1 protein protein human Homo sapiens Human MACWPQLRLLLWKNLTFRRRQTCQLLLEVAWPLFIFLILISVRLSYPPYEQHECHFPNKAMPSAGTLPWVQGIICNANNPCFRYPTPGEAPGVVGNFNKSIVARLFSDARRLLLYSQKDTSMKDMRKVLRTLQQIKKSSSNLKLQDFLVDNETFSGFLYHNLSLPKSTVDKMLRADVILHKVFLQGYQLHLTSLCNGSKSEEMIQLGDQEVSELCGLPREKLAAAERVLRSNMDILKPILRTLNSTSPFPSKELAEATKTLLHSLGTLAQELFSMRSWSDMRQEVMFLTNVNSSSSSTQIYQAVSRIVCGHPEGGGLKIKSLNWYEDNNYKALFGGNGTEEDAETFYDNSTTPYCNDLMKNLESSPLSRIIWKALKPLLVGKILYTPDTPATRQVMAEVNKTFQELAVFHDLEGMWEELSPKIWTFMENSQEMDLVRMLLDSRDNDHFWEQQLDGLDWTAQDIVAFLAKHPEDVQSSNGSVYTWREAFNETNQAIRTISRFMECVNLNKLEPIATEVWLINKSMELLDERKFWAGIVFTGITPGSIELPHHVKYKIRMDIDNVERTNKIKDGYWDPGPRADPFEDMRYVWGGFAYLQDVVEQAIIRVLTGTEKKTGVYMQQMPYPCYVDDIFLRVMSRSMPLFMTLAWIYSVAVIIKGIVYEKEARLKETMRIMGLDNSILWFSWFISSLIPLLVSAGLLVVILKLGNLLPYSDPSVVFVFLSVFAVVTILQCFLISTLFSRANLAAACGGIIYFTLYLPYVLCVAWQDYVGFTLKIFASLLSPVAFGFGCEYFALFEEQGIGVQWDNLFESPVEEDGFNLTTSVSMMLFDTFLYGVMTWYIEAVFPGQYGIPRPWYFPCTKSYWFGEESDEKSHPGSNQKRISEICMEEEPTHLKLGVSIQNLVKVYRDGMKVAVDGLALNFYEGQITSFLGHNGAGKTTTMSILTGLFPPTSGTAYILGKDIRSEMSTIRQNLGVCPQHNVLFDMLTVEEHIWFYARLKGLSEKHVKAEMEQMALDVGLPSSKLKSKTSQLSGGMQRKLSVALAFVGGSKVVILDEPTAGVDPYSRRGIWELLLKYRQGRTIILSTHHMDEADVLGDRIAIISHGKLCCVGSSLFLKNQLGTGYYLTLVKKDVESSLSSCRNSSSTVSYLKKEDSVSQSSSDAGLGSDHESDTLTIDVSAISNLIRKHVSEARLVEDIGHELTYVLPYEAAKEGAFVELFHEIDDRLSDLGISSYGISETTLEEIFLKVAEESGVDAETSDGTLPARRNRRAFGDKQSCLRPFTEDDAADPNDSDIDPESRETDLLSGMDGKGSYQVKGWKLTQQQFVALLWKRLLIARRSRKGFFAQIVLPAVFVCIALVFSLIVPPFGKYPSLELQPWMYNEQYTFVSNDAPEDTGTLELLNALTKDPGFGTRCMEGNPIPDTPCQAGEEEWTTAPVPQTIMDLFQNGNWTMQNPSPACQCSSDKIKKMLPVCPPGAGGLPPPQRKQNTADILQDLTGRNISDYLVKTYVQIIAKSLKNKIWVNEFRYGGFSLGVSNTQALPPSQEVNDAIKQMKKHLKLAKDSSADRFLNSLGRFMTGLDTKNNVKVWFNNKGWHAISSFLNVINNAILRANLQKGENPSHYGITAFNHPLNLTKQQLSEVALMTTSVDVLVSICVIFAMSFVPASFVVFLIQERVSKAKHLQFISGVKPVIYWLSNFVWDMCNYVVPATLVIIIFICFQQKSYVSSTNLPVLALLLLLYGWSITPLMYPASFVFKIPSTAYVVLTSVNLFIGINGSVATFVLELFTDNKLNNINDILKSVFLIFPHFCLGRGLIDMVKNQAMADALERFGENRFVSPLSWDLVGRNLFAMAVEGVVFFLITVLIQYRFFIRPRPVNAKLSPLNDEDEDVRRERQRILDGGGQNDILEIKELTKIYRRKRKPAVDRICVGIPPGECFGLLGVNGAGKSSTFKMLTGDTTVTRGDAFLNKNSILSNIHEVHQNMGYCPQFDAITELLTGREHVEFFALLRGVPEKEVGKVGEWAIRKLGLVKYGEKYAGNYSGGNKRKLSTAMALIGGPPVVFLDEPTTGMDPKARRFLWNCALSVVKEGRSVVLTSHSMEECEALCTRMAIMVNGRFRCLGSVQHLKNRFGDGYTIVVRIAGSNPDLKPVQDFFGLAFPGSVLKEKHRNMLQYQLPSSLSSLARIFSILSQSKKRLHIEDYSVSQTTLDQVFVNFAKDQSDDDHLKDLSLHKNQTVVDVAVLTSFLQDEKVKESYV stx12_human Syntaxin-12 STX12 protein protein human Homo sapiens Human MSYGPLDMYRNPGPSGPQLRDFSSIIQTCSGNIQRISQATAQIKNLMSQLGTKQDSSKLQENLQQLQHSTNQLAKETNELLKELGSLPLPLSTSEQRQQRLQKERLMNDFSAALNNFQAVQRRVSEKEKESIARARAGSRLSAEERQREEQLVSFDSHEEWNQMQSQEDEVAITEQDLELIKERETAIRQLEADILDVNQIFKDLAMMIHDQGDLIDSIEANVESSEVHVERATEQLQRAAYYQKKSRKKMCILVLVLSVIILILGLIIWLVYKTK flot1_human Flotillin-1 FLOT1 protein protein human Homo sapiens Human MFFTCGPNEAMVVSGFCRSPPVMVAGGRVFVLPCIQQIQRISLNTLTLNVKSEKVYTRHGVPISVTGIAQVKIQGQNKEMLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA abca1-bloc1s6-1 1533251 n/a 1533256 unspecified role unspecified role bait bait n/a 1533257 unspecified role unspecified role prey prey physical association physical association false false false Fig 2A bloc1s6-abca1 1533251 n/a 1533256 unspecified role unspecified role prey prey n/a 1533257 unspecified role unspecified role bait bait physical association physical association false false false Fig 2B abca1-flot1-1 1533251 n/a 1533256 unspecified role unspecified role bait bait n/a 1533258 unspecified role unspecified role prey prey physical association physical association false false false Fig. 6B abca1-stx12-1 1533251 n/a 1533256 unspecified role unspecified role bait bait n/a 1533257 unspecified role unspecified role prey prey physical association physical association false false false Fig 5B stx12-abca1 1533251 n/a 1533257 unspecified role unspecified role bait bait n/a 1533256 unspecified role unspecified role prey prey physical association physical association false false false Fig 5B bloc1s6-abca1-1 1533252 n/a 1533256 unspecified role unspecified role prey prey n/a 1533257 unspecified role unspecified role bait bait physical association physical association false false false Fig 2B abca1-bloc1s6-2 1533252 n/a 1533256 unspecified role unspecified role bait bait n/a 1533257 unspecified role unspecified role prey prey physical association physical association false false false Fig 2A abca1-bloc1s6-6 1533253 n/a 1533257 unspecified role unspecified role neutral component neutral component n/a 1533256 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 3A,B,C abca1-bloc1s6-5 1533254 n/a 1533256 unspecified role unspecified role neutral component neutral component n/a 1533257 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 3D, E, F abca1-flot1-5 1533255 n/a 1533258 unspecified role unspecified role neutral component neutral component n/a 1533257 unspecified role unspecified role neutral component neutral component n/a 1533256 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig.6A The treatment with Lubrol WX showed colocalization of ABCA1, Syntaxin 13 and Flotillin-1 in the rafts fraction. The treatment with Triton X-100 showed colocalization of ABCA1, Syntaxin 13 and Flotillin-1 in the soluble membrane proteins fraction.