bhf-ucl fang-2000-1 Phosphorylation and inactivation of glycogen synthase kinase 3 by protein kinase A. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Fang X., Yu S.X., Lu Y., Bast R.C., Woodgett J.R., Mills G.B. Cardiac - Interactions involving cardiac related proteins gmills@notes.mdacc.tmc.edu unable to determine species of cAMP-dependent protein kinase A in PKA assay kit (Upstate Biotechnology), therefore Fig 4B not annotated Anti-GSK-3α (sheep anti-rat GSK-3α; Upstate Biotechnology) Anti-GSK-3β (mouse monoclonal anti-rat GSK-3β; Transduction Laboratories, Lexington, KY) Rabbit polyclonal antibodies against PKAc or the PKA regulatory subunit RII (PKA RII) (Santa Cruz Biotechnology) were used for PKAc or PKA RII immunoprecipitation and Western blotting. Antibodies to PKA RII do not identify specific isoforms therefore PPIs using this antibody not annotated. PKB was immunoprecipitated with a sheep polyclonal anti-rat PKB (Upstate Biotechnology). Dr. B. Su (M. D. Anderson Cancer Center) provided anti-HA monoclonal antibody Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2000 imex curation Only protein-protein interactions fang-2000-3 Phosphorylation and inactivation of glycogen synthase kinase 3 by protein kinase A. mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Fang X., Yu S.X., Lu Y., Bast R.C., Woodgett J.R., Mills G.B. Cardiac - Interactions involving cardiac related proteins gmills@notes.mdacc.tmc.edu unable to determine species of cAMP-dependent protein kinase A in PKA assay kit (Upstate Biotechnology), therefore Fig 4B not annotated Anti-GSK-3α (sheep anti-rat GSK-3α; Upstate Biotechnology) Anti-GSK-3β (mouse monoclonal anti-rat GSK-3β; Transduction Laboratories, Lexington, KY) Rabbit polyclonal antibodies against PKAc or the PKA regulatory subunit RII (PKA RII) (Santa Cruz Biotechnology) were used for PKAc or PKA RII immunoprecipitation and Western blotting. Antibodies to PKA RII do not identify specific isoforms therefore PPIs using this antibody not annotated. PKB was immunoprecipitated with a sheep polyclonal anti-rat PKB (Upstate Biotechnology). Dr. B. Su (M. D. Anderson Cancer Center) provided anti-HA monoclonal antibody Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2000 imex curation Only protein-protein interactions fang-2000-2 Phosphorylation and inactivation of glycogen synthase kinase 3 by protein kinase A. rat-rat-1 Rattus norvegicus embryonic fibroblast cells rat-1 Rat-1 embryonic fibroblasts anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Fang X., Yu S.X., Lu Y., Bast R.C., Woodgett J.R., Mills G.B. Cardiac - Interactions involving cardiac related proteins gmills@notes.mdacc.tmc.edu unable to determine species of cAMP-dependent protein kinase A in PKA assay kit (Upstate Biotechnology), therefore Fig 4B not annotated Anti-GSK-3α (sheep anti-rat GSK-3α; Upstate Biotechnology) Anti-GSK-3β (mouse monoclonal anti-rat GSK-3β; Transduction Laboratories, Lexington, KY) Rabbit polyclonal antibodies against PKAc or the PKA regulatory subunit RII (PKA RII) (Santa Cruz Biotechnology) were used for PKAc or PKA RII immunoprecipitation and Western blotting. Antibodies to PKA RII do not identify specific isoforms therefore PPIs using this antibody not annotated. PKB was immunoprecipitated with a sheep polyclonal anti-rat PKB (Upstate Biotechnology). Dr. B. Su (M. D. Anderson Cancer Center) provided anti-HA monoclonal antibody Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2000 imex curation Only protein-protein interactions fang-2000-4 Phosphorylation and inactivation of glycogen synthase kinase 3 by protein kinase A. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Fang X., Yu S.X., Lu Y., Bast R.C., Woodgett J.R., Mills G.B. Cardiac - Interactions involving cardiac related proteins gmills@notes.mdacc.tmc.edu unable to determine species of cAMP-dependent protein kinase A in PKA assay kit (Upstate Biotechnology), therefore Fig 4B not annotated Anti-GSK-3α (sheep anti-rat GSK-3α; Upstate Biotechnology) Anti-GSK-3β (mouse monoclonal anti-rat GSK-3β; Transduction Laboratories, Lexington, KY) Rabbit polyclonal antibodies against PKAc or the PKA regulatory subunit RII (PKA RII) (Santa Cruz Biotechnology) were used for PKAc or PKA RII immunoprecipitation and Western blotting. Antibodies to PKA RII do not identify specific isoforms therefore PPIs using this antibody not annotated. PKB was immunoprecipitated with a sheep polyclonal anti-rat PKB (Upstate Biotechnology). Dr. B. Su (M. D. Anderson Cancer Center) provided anti-HA monoclonal antibody Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2000 imex curation Only protein-protein interactions kapca_human cAMP-dependent protein kinase catalytic subunit alpha PRKACA PKACA protein protein human Homo sapiens Human MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRVMLVKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF gsk3a_rat Glycogen synthase kinase-3 alpha Gsk3a Factor A Serine/threonine-protein kinase GSK3A protein protein rat Rattus norvegicus (Rat) Rat MSGGGPSGGGPGGSGRARTSSFAEPGGGGGGGGGGPGGSASGPGGTGGGKASVGAMGGGVGASSSGGGPSGSGGGGSGGPGAGTSFPPPGVKLGRDSGKVTTVVATLGQGPERSQEVAYTDIKVIGNGSFGVVYQARLAETRELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDELYLNLVLEYVPETVYRVARHFTKAKLIIPIIYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFKSRTPPEAIALCSSLLEYTPSSRLSPLEACAHSFFDELRSLGTQLPNNRPLPPLFNFSPGELSIQPSLNAILIPPHLRSPSGPATLTSSSQALTETQTGQDWQAPDATPTLTNSS gsk3b_human Glycogen synthase kinase-3 beta GSK3B Serine/threonine-protein kinase GSK3B protein protein human Homo sapiens Human MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASASASNST kapca_mouse cAMP-dependent protein kinase catalytic subunit alpha Prkaca Pkaca protein protein mouse Mus musculus Mouse MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTGSFGRVMLVKHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVAGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTEF gsk3b_mouse Glycogen synthase kinase-3 beta Gsk3b Serine/threonine-protein kinase GSK3B protein protein mouse Mus musculus Mouse MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASPPANATAASDTNAGDRGQTNNAASASASNST kapca_rat cAMP-dependent protein kinase catalytic subunit alpha Prkaca protein protein rat Rattus norvegicus (Rat) Rat MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWEDPSQNTAQLDHFDRIKTLGTGSFGRVMLVKHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTEF gsk3a-prkaca 1438108 n/a 1438112 polyclonal western polyclonal antibody western blot polyclonal western unspecified role unspecified role prey prey cell lysate cell lysate n/a 1438113 polyclonal western polyclonal antibody western blot polyclonal western unspecified role unspecified role bait bait cell lysate cell lysate physical association physical association false false false Fig 3A gsk3b-prkaca 1438108 n/a 1438112 polyclonal western polyclonal antibody western blot polyclonal western unspecified role unspecified role prey prey cell lysate cell lysate n/a 1438114 monoclonal western monoclonal antibody western blot monoclonal western unspecified role unspecified role bait bait cell lysate cell lysate ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 3A prkaca-gsk3b 1438108 n/a 1438112 polyclonal western polyclonal antibody western blot polyclonal western unspecified role unspecified role bait bait cell lysate cell lysate n/a 1438114 monoclonal western monoclonal antibody western blot monoclonal western unspecified role unspecified role prey prey cell lysate cell lysate ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 3B gsk3b-prkaca-4 1438108 n/a 1438112 polyclonal western polyclonal antibody western blot polyclonal western unspecified role unspecified role prey prey cell lysate cell lysate n/a 1438114 monoclonal western monoclonal antibody western blot monoclonal western unspecified role unspecified role bait bait cell lysate cell lysate physical association physical association false false false Fig 3C forskolin gsk3b-prkaca-2 1438109 n/a 1438115 polyclonal western polyclonal antibody western blot polyclonal western unspecified role unspecified role prey prey cell lysate cell lysate n/a 1438114 monoclonal western monoclonal antibody western blot monoclonal western unspecified role unspecified role bait bait cell lysate cell lysate ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 3A gsk3b-prkaca-6 1438109 n/a 1438116 monoclonal western monoclonal antibody western blot monoclonal western unspecified role unspecified role bait bait cell lysate cell lysate n/a 1438115 polyclonal western polyclonal antibody western blot polyclonal western unspecified role unspecified role prey prey cell lysate cell lysate physical association physical association false false false Fig 3C forskolin gsk3a-prkaca-2 1438110 n/a 1438117 polyclonal western polyclonal antibody western blot polyclonal western unspecified role unspecified role prey prey cell lysate cell lysate n/a 1438113 polyclonal western polyclonal antibody western blot polyclonal western unspecified role unspecified role bait bait cell lysate cell lysate physical association physical association false false false Fig 3A gsk3b-prkaca-3 1438111 n/a 1438112 polyclonal western polyclonal antibody western blot polyclonal western unspecified role unspecified role prey prey cell lysate cell lysate n/a 1438114 monoclonal western monoclonal antibody western blot monoclonal western unspecified role unspecified role bait bait cell lysate cell lysate ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 3B