IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ham-2016-1 human-kelly human KELLY- Neuroblastoma cell line with ampification of N-MYC Human kelly KELLY- Neuroblastoma cell line with ampification of N-MYC https://www.dsmz.de/catalogues/details/culture/ACC-355.html ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Ham J., Costa C., Sano R., Lochmann TL., Sennott EM., Patel NU., Dastur A., Gomez-Caraballo M., Krytska K., Hata AN., Floros KV., Hughes MT., Jakubik CT., Heisey DA., Ferrell JT., Bristol ML., March RJ., Yates C., Hicks MA., Nakajima W., Gowda M., Windle BE., Dozmorov MG., Garnett MJ., McDermott U., Harada H., Taylor SM., Morgan IM., Benes CH., Engelman JA., Mossé YP., Faber AC. Cancer - Interactions investigated in the context of cancer The GFP-IRES-MCL-1 pLENTI plasmid was found to be of Murine origin -pmid:21613222 (Warr et al., 2011). The provider of the plasmid was a Dr Gordon Shore, as stated in the Faber - 2015 reference (not Faber - 2014 as stated.) acfaber@vcu.edu Cancer cell 2016 Only protein-protein interactions imex curation imex curation ham-2016-2 human-sk-n-dz human-SK-N-DZ;neuroblastoma cell line from a poorly differentiated bone marrow metastasis. Human sk-n-dz SK-N-DZ -neuroblastoma cell line from a poorly differentiated bone marrow metastasis http://www.lgcstandards-atcc.org/products/all/CRL-2149 ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Ham J., Costa C., Sano R., Lochmann TL., Sennott EM., Patel NU., Dastur A., Gomez-Caraballo M., Krytska K., Hata AN., Floros KV., Hughes MT., Jakubik CT., Heisey DA., Ferrell JT., Bristol ML., March RJ., Yates C., Hicks MA., Nakajima W., Gowda M., Windle BE., Dozmorov MG., Garnett MJ., McDermott U., Harada H., Taylor SM., Morgan IM., Benes CH., Engelman JA., Mossé YP., Faber AC. Cancer - Interactions investigated in the context of cancer The GFP-IRES-MCL-1 pLENTI plasmid was found to be of Murine origin -pmid:21613222 (Warr et al., 2011). The provider of the plasmid was a Dr Gordon Shore, as stated in the Faber - 2015 reference (not Faber - 2014 as stated.) acfaber@vcu.edu Cancer cell 2016 Only protein-protein interactions imex curation imex curation ham-2016-3 human-sk_n_be(2) Human caucasian neuroblastoma cell line sk_n_be(2) Human neuroblastoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ham J., Costa C., Sano R., Lochmann TL., Sennott EM., Patel NU., Dastur A., Gomez-Caraballo M., Krytska K., Hata AN., Floros KV., Hughes MT., Jakubik CT., Heisey DA., Ferrell JT., Bristol ML., March RJ., Yates C., Hicks MA., Nakajima W., Gowda M., Windle BE., Dozmorov MG., Garnett MJ., McDermott U., Harada H., Taylor SM., Morgan IM., Benes CH., Engelman JA., Mossé YP., Faber AC. Cancer - Interactions investigated in the context of cancer acfaber@vcu.edu Cancer cell 2016 Only protein-protein interactions imex curation imex curation ham-2016-4 human-sk-n-dz human-SK-N-DZ;neuroblastoma cell line from a poorly differentiated bone marrow metastasis. Human sk-n-dz SK-N-DZ -neuroblastoma cell line from a poorly differentiated bone marrow metastasis http://www.lgcstandards-atcc.org/products/all/CRL-2149 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ham J., Costa C., Sano R., Lochmann TL., Sennott EM., Patel NU., Dastur A., Gomez-Caraballo M., Krytska K., Hata AN., Floros KV., Hughes MT., Jakubik CT., Heisey DA., Ferrell JT., Bristol ML., March RJ., Yates C., Hicks MA., Nakajima W., Gowda M., Windle BE., Dozmorov MG., Garnett MJ., McDermott U., Harada H., Taylor SM., Morgan IM., Benes CH., Engelman JA., Mossé YP., Faber AC. Cancer - Interactions investigated in the context of cancer acfaber@vcu.edu Cancer cell 2016 Only protein-protein interactions imex curation imex curation ham-2016-5 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Ham J., Costa C., Sano R., Lochmann TL., Sennott EM., Patel NU., Dastur A., Gomez-Caraballo M., Krytska K., Hata AN., Floros KV., Hughes MT., Jakubik CT., Heisey DA., Ferrell JT., Bristol ML., March RJ., Yates C., Hicks MA., Nakajima W., Gowda M., Windle BE., Dozmorov MG., Garnett MJ., McDermott U., Harada H., Taylor SM., Morgan IM., Benes CH., Engelman JA., Mossé YP., Faber AC. Cancer - Interactions investigated in the context of cancer Western Blotting was used for Participant Detection, as stated in the main body of the paper in reference to Fig S5G. From the antibody datasheet (eIF4G (cat #2469) it was determined that "eIF4G" refers to the molecule EIF4G1 as this is the only species of EIF4G that has a Gly at residue 188. acfaber@vcu.edu Cancer cell 2016 Only protein-protein interactions imex curation imex curation apr_human_gene Phorbol-12-myristate-13-acetate-induced protein 1 PMAIP1 NOXA gene gene human Homo sapiens Human mycn_human N-myc proto-oncogene protein MYCN NMYC BHLHE37 Class E basic helix-loop-helix protein 37 protein protein human Homo sapiens Human MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC mdm2_human_gene Human MDM2 gene gene gene human Homo sapiens Human mir17hg_human_gene MIR17HG_human_gene gene gene human Homo sapiens Human b2l11_human Bcl-2-like protein 11 BCL2L11 BIM Bcl2-interacting mediator of cell death protein protein human Homo sapiens Human MAKQPSDVSSECDREGRQLQPAERPPQLRPGAPTSLQTEPQGNPEGNHGGEGDSCPHGSPQGPLAPPASPGPFATRSPLFIFMRRSSLLSRSSSGYFSFDTDRSPAPMSCDKSTQTPSPPCQAFNHYLSAMASMRQAEPADMRPEIWIAQELRRIGDEFNAYYARRVFLNNYQAAEDHPRMVILRLLRYIVRLVWRMH mcl1_human Induced myeloid leukemia cell differentiation protein Mcl-1 Bcl-2-related protein EAT/mcl1 mcl1/EAT MCL1 BCL2L3 Bcl-2-like protein 3 protein protein human Homo sapiens Human MFGLKRNAVIGLNLYCGGAGLGAGSGGATRPGGRLLATEKEASARREIGGGEAGAVIGGSAGASPPSTLTPDSRRVARPPPIGAEVPDVTATPARLLFFAPTRRAAPLEEMEAPAADAIMSPEEELDGYEPEPLGKRPAVLPLLELVGESGNNTSTDGSLPSTPPPAEEEEDELYRQSLEIISRYLREQATGAKDTKPMGRSGATSRKALETLRRVGDGVQRNHETAFQGMLRKLDIKNEDDVKSLSRVMIHVFSDGVTNWGRIVTLISFGAFVAKHLKTINQESCIEPLAESITDVLVRTKRDWLVKQRGWDGFVEFFHVEDLEGGIRNVLLAFAGVAGVGAGLAYLIR if4g1_human Eukaryotic translation initiation factor 4 gamma 1 p220 EIF4G1 EIF4G EIF4GI EIF4F protein protein human Homo sapiens Human MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPTPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN if4e_human Eukaryotic translation initiation factor 4E mRNA cap-binding protein eIF-4F 25 kDa subunit EIF4E EIF4EL1 EIF4F protein protein human Homo sapiens Human MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV 4ebp1_human Eukaryotic translation initiation factor 4E-binding protein 1 Phosphorylated heat- and acid-stable protein regulated by insulin 1 EIF4EBP1 protein protein human Homo sapiens Human MSGGSSCSQTPSRAIPATRRVVLGDGVQLPPGDYSTTPGGTLFSTTPGGTRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI 7-methyl-gtp 7-methyl-GTP small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) mycn-pmaip1-1 1915844 N/A 1915849 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1915850 unspecified role unspecified role bait bait association association false false false 2J P04198 increases expression at RNA level of ENSG00000141682 P04198 increases expression at protein level of ENSG00000141682 mycn-mdm2_human_gene-1 1915844 N/A 1915851 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1915850 unspecified role unspecified role bait bait association association false false false 2J-2 primers given for MDM2 are too short to give a Blat result. mycn-mir17hg_human_gene-1 1915844 N/A 1915852 unspecified role unspecified role prey prey intron_1_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1915850 unspecified role unspecified role bait bait association association false false false 2J-3 mycn-mdm2_human_gene-2 1915845 N/A 1915850 unspecified role unspecified role bait bait N/A 1915851 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 2J-2 primers given for MDM2 are too short to give a Blat result. mycn-mir17hg_human_gene-2 1915845 N/A 1915852 unspecified role unspecified role prey prey intron_1_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1915850 unspecified role unspecified role bait bait association association false false false 2J-3 mycn-pmaip1-2 1915845 N/A 1915849 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1915850 unspecified role unspecified role bait bait association association false false false 2J P04198 increase expression at RNA level of ENSG00000141682 P04198 increase expression at protein level of ENSG00000141682 mcl1-bcl2l11-11 1915846 N/A 1915853 unspecified role unspecified role prey prey N/A 1915854 unspecified role unspecified role bait bait physical association physical association false false false 5E ABT-199 (Venetoclax) -a BCL-2 inhibitor, encouraging apoptosis. MLN8237-an Aurora Kinase A inhibitor mcl1-bcl2l11-12 1915846 N/A 1915853 unspecified role unspecified role bait bait N/A 1915854 unspecified role unspecified role prey prey physical association physical association false false false Supp 6D ABT-199 (Venetoclax) -a BCL-2 inhibitor, encouraging apoptosis. MLN8237-an Aurora Kinase A inhibitor mcl1-bcl2l11-10 1915847 N/A 1915854 unspecified role unspecified role bait bait N/A 1915853 unspecified role unspecified role prey prey physical association physical association false false false 5E ABT-199 (Venetoclax) -a BCL-2 inhibitor, encouraging apoptosis. MLN8237-an Aurora Kinase A inhibitor mcl1-bcl2l11-13 1915847 N/A 1915854 unspecified role unspecified role prey prey N/A 1915853 unspecified role unspecified role bait bait physical association physical association false false false Supp 6D ABT-199 (Venetoclax) -a BCL-2 inhibitor, encouraging apoptosis. MLN8237-an Aurora Kinase A inhibitor 7_methyl_gtp-eif4e-1 1915848 N/A 1915855 unspecified role unspecified role prey prey human-kelly human KELLY- Neuroblastoma cell line with ampification of N-MYC Human kelly KELLY- Neuroblastoma cell line with ampification of N-MYC https://www.dsmz.de/catalogues/details/culture/ACC-355.html N/A 1915856 unspecified role unspecified role prey prey human-kelly human KELLY- Neuroblastoma cell line with ampification of N-MYC Human kelly KELLY- Neuroblastoma cell line with ampification of N-MYC https://www.dsmz.de/catalogues/details/culture/ACC-355.html N/A 1915857 unspecified role unspecified role prey prey human-kelly human KELLY- Neuroblastoma cell line with ampification of N-MYC Human kelly KELLY- Neuroblastoma cell line with ampification of N-MYC https://www.dsmz.de/catalogues/details/culture/ACC-355.html N/A 1915858 unspecified role unspecified role bait bait association association false false false Supp 5G MLN8237; 4EBP1:eIF4E complex formation is increased MLN8237 (Aurora A inhibitor) ;eIF4G:eIF4E complex formation decreases 7_methyl_gtp-eif4e-2 1915848 N/A 1915855 unspecified role unspecified role prey prey human-sk_n_be(2) Human caucasian neuroblastoma cell line sk_n_be(2) Human neuroblastoma cell line N/A 1915856 unspecified role unspecified role prey prey human-sk_n_be(2) Human caucasian neuroblastoma cell line sk_n_be(2) Human neuroblastoma cell line N/A 1915857 unspecified role unspecified role prey prey human-sk_n_be(2) Human caucasian neuroblastoma cell line sk_n_be(2) Human neuroblastoma cell line N/A 1915858 unspecified role unspecified role bait bait association association false false false Supp 5G-2 MLN8237; 4EBP1:eIF4E complex formation is increased MLN8237 (Aurora A inhibitor) ;eIF4G:eIF4E complex formation decreases