IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ronca-2015-1 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Ronca R., Giacomini A., Di Salle E., Coltrini D., Pagano K., Ragona L., Matarazzo S., Rezzola S., Maiolo D., Torrella R., Moroni E., Mazzieri R., Escobar G., Mor M., Colombo G., Presta M. Cancer - Interactions investigated in the context of cancer It is stated that all FGFs were obtained from Peprotech - however FGF3 and FGF22 could not be found on their website. The authors have stated that sFGFR1(IIIc)/Fc chimera was supplied from Reliatech;blasting the sequence shows the N-terminal FGFR1 part of this protein corresponds to Human FGFR1, P11362 (canonical isoform P11362-1) amino acids 22-376, the extra cellular domain, which has been entered here. The authors also state that FGF2 was “home made” (self produced) and the full length molecule of the 18kDa isoform of FGF2. This would correspond to FGF2 Isoform 3 (identifier: P09038-2) which is made up of 155 amino acids. marco.presta@unibs.it Cancer cell 2015 Only protein-protein interactions imex curation imex curation ronca-2015-3 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Ronca R., Giacomini A., Di Salle E., Coltrini D., Pagano K., Ragona L., Matarazzo S., Rezzola S., Maiolo D., Torrella R., Moroni E., Mazzieri R., Escobar G., Mor M., Colombo G., Presta M. Cancer - Interactions investigated in the context of cancer The authors have stated that sFGFR1(IIIc)/Fc chimera was supplied from Reliatech;blasting the sequence shows the N-terminal FGFR1 part of this protein corresponds to Human FGFR1, P11362 (canonical isoform P11362-1) amino acids 22-376, the extra cellular domain, which has been entered here. The authors also state that FGF2 was “home made” (self produced) and the full length molecule of the 18kDa isoform of FGF2. This would correspond to FGF2 Isoform 3 (identifier: P09038-2) which is made up of 155 amino acids. marco.presta@unibs.it Cancer cell 2015 Only protein-protein interactions imex curation imex curation ronca-2015-2 in vitro In vitro biosensor biosensor predetermined predetermined participant predetermined Ronca R., Giacomini A., Di Salle E., Coltrini D., Pagano K., Ragona L., Matarazzo S., Rezzola S., Maiolo D., Torrella R., Moroni E., Mazzieri R., Escobar G., Mor M., Colombo G., Presta M. Cancer - Interactions investigated in the context of cancer COntact Angle MOlecular REcognition (CONAMORE). Molecular interactions supported by a solid phase result in a shift in the solid-solution surface tension equilibrium, which can be tracked by the alteration of of the contact angle. Methodology is similar to SPR with one interactor immobilised on a chip and the alteration of contact angle monitored optically after addition of proposed interactor(s) in solution. The authors have stated that sFGFR1(IIIc)/Fc chimera was supplied from Reliatech;blasting the sequence shows the N-terminal FGFR1 part of this protein corresponds to Human FGFR1, P11362 (canonical isoform P11362-1) amino acids 22-376, the extra cellular domain, which has been entered here. The authors also state that FGF2 was “home made” (self produced) and the full length molecule of the 18kDa isoform of FGF2. This would correspond to FGF2 Isoform 3 (identifier: P09038-2) which is made up of 155 amino acids. marco.presta@unibs.it Cancer cell 2015 Only protein-protein interactions imex curation imex curation p09038-2 Fibroblast growth factor 2 FGF2 Basic fibroblast growth factor FGFB Heparin-binding growth factor 2 protein protein human Homo sapiens Human MAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS heparin Heparin small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) fgfr1_human Fibroblast growth factor receptor 1 FGFR1 FGFBR FLG FLT2 Fms-like tyrosine kinase 2 Proto-oncogene c-Fgr BFGFR CEK HBGFR Basic fibroblast growth factor receptor 1 N-sam protein protein human Homo sapiens Human MWSWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLYLEIIIYCTGAFLISCMVGSVIVYKMKSGTKKSDFHSQMAVHKLAKSIPLRRQVTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSEYELPEDPRWELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSMPLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGGLKRR fgf2_human Fibroblast growth factor 2 Basic fibroblast growth factor FGF2 FGFB Heparin-binding growth factor 2 protein protein human Homo sapiens Human MVGVGGGDVEDVTPRPGGCQISGRGARGCNGIPGAAAWEAALPRRRPRRHPSVNPRSRAAGSPRTRGRRTEERPSGSRLGDRGRGRALPGGRLGGRGRGRAPERVGGRGRGRGTAAPRAAPAARGSRPGPAGTMAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS nsc12 NSC12 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) fgf3_human Fibroblast growth factor 3 FGF3 INT2 Heparin-binding growth factor 3 Proto-oncogene Int-2 protein protein human Homo sapiens Human MGLIWLLLLSLLEPGWPAAGPGARLRRDAGGRGGVYEHLGGAPRRRKLYCATKYHLQLHPSGRVNGSLENSAYSILEITAVEVGIVAIRGLFSGRYLAMNKRGRLYASEHYSAECEFVERIHELGYNTYASRLYRTVSSTPGARRQPSAERLWYVSVNGKGRPRRGFKTRRTQKSSLFLPRVLDHRDHEMVRQLQSGLPRPPGKGVQPRRRRQKQSPDNLEPSHVQASRLGSQLEASAH p08620-1 Fibroblast growth factor 4 FGF4 HST HSTF1 KS3 Heparin secretory-transforming protein 1 Heparin-binding growth factor 4 Transforming protein KS3 protein protein human Homo sapiens Human MSGPGTAAVALLPAVLLALLAPWAGRGGAAAPTAPNGTLEAELERRWESLVALSLARLPVAAQPKEAAVQSGAGDYLLGIKRLRRLYCNVGIGFHLQALPDGRIGGAHADTRDSLLELSPVERGVVSIFGVASRFFVAMSSKGKLYGSPFFTDECTFKEILLPNNYNAYESYKYPGMFIALSKNGKTKKGNRVSPTMKVTHFLPRL fgf6_human Fibroblast growth factor 6 FGF6 HST2 HSTF2 Heparin secretory-transforming protein 2 Heparin-binding growth factor 6 protein protein human Homo sapiens Human MALGQKLFITMSRGAGRLQGTLWALVFLGILVGMVVPSPAGTRANNTLLDSRGWGTLLSRSRAGLAGEIAGVNWESGYLVGIKRQRRLYCNVGIGFHLQVLPDGRISGTHEENPYSLLEISTVERGVVSLFGVRSALFVAMNSKGRLYATPSFQEECKFRETLLPNNYNAYESDLYQGTYIALSKYGRVKRGSKVSPIMTVTHFLPRI p55075-3 Fibroblast growth factor 8 FGF8 AIGF Androgen-induced growth factor Heparin-binding growth factor 8 protein protein human Homo sapiens Human MGSPRSALSCLLLHLLVLCLQAQVTVQSSPNFTQHVREQSLVTDQLSRRLIRTYQLYSRTSGKHVQVLANKRINAMAEDGDPFAKLIVETDTFGSRVRVRGAETGLYICMNKKGKLIAKSNGKGKDCVFTEIVLENNYTALQNAKYEGWYMAFTRKGRPRKGSKTRQHQREVHFMKRLPRGHHTTEQSLRFEFLNYPPFTRSLRGSQRTWAPEPR fgf16_human Fibroblast growth factor 16 FGF16 protein protein human Homo sapiens Human MAEVGGVFASLDWDLHGFSSSLGNVPLADSPGFLNERLGQIEGKLQRGSPTDFAHLKGILRRRQLYCRTGFHLEIFPNGTVHGTRHDHSRFGILEFISLAVGLISIRGVDSGLYLGMNERGELYGSKKLTRECVFREQFEENWYNTYASTLYKHSDSERQYYVALNKDGSPREGYRTKRHQKFTHFLPRPVDPSKLPSMSRDLFHYR fgf18_human Fibroblast growth factor 18 FGF18 zFGF5 UNQ420/PRO856 protein protein human Homo sapiens Human MYSAPSACTCLCLHFLLLCFQVQVLVAEENVDFRIHVENQTRARDDVSRKQLRLYQLYSRTSGKHIQVLGRRISARGEDGDKYAQLLVETDTFGSQVRIKGKETEFYLCMNRKGKLVGKPDGTSKECVFIEKVLENNYTALMSAKYSGWYVGFTKKGRPRKGPKTRENQQDVHFMKRYPKGQPELQKPFKYTTVTKRSRRIRPTHPA fgf20_human Fibroblast growth factor 20 FGF20 protein protein human Homo sapiens Human MAPLAEVGGFLGGLEGLGQQVGSHFLLPPAGERPPLLGERRSAAERSARGGPGAAQLAHLHGILRRRQLYCRTGFHLQILPDGSVQGTRQDHSLFGILEFISVAVGLVSIRGVDSGLYLGMNDKGELYGSEKLTSECIFREQFEENWYNTYSSNIYKHGDTGRRYFVALNKDGTPRDGARSKRHQKFTHFLPRPVDPERVPELYKDLLMYT fgf22_human Fibroblast growth factor 22 FGF22 UNQ2500/PRO5800 protein protein human Homo sapiens Human MRRRLWLGLAWLLLARAPDAAGTPSASRGPRSYPHLEGDVRWRRLFSSTHFFLRVDPGGRVQGTRWRHGQDSILEIRSVHVGVVVIKAVSSGFYVAMNRRGRLYGSRLYTVDCRFRERIEENGHNTYASQRWRRRGQPMFLALDRRGGPRPGGRTRRYHLSAHFLPVLVS fgfr2_human Fibroblast growth factor receptor 2 FGFR2 BEK KSAM Keratinocyte growth factor receptor KGFR K-sam protein protein human Homo sapiens Human MVSWGRFICLVVVTMATLSLARPSFSLVEDTTLEPEEPPTKYQISQPEVYVAAPGESLEVRCLLKDAAVISWTKDGVHLGPNNRTVLIGEYLQIKGATPRDSGLYACTASRTVDSETWYFMVNVTDAISSGDDEDDTDGAEDFVSENSNNKRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGGNPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCVVENEYGSINHTYHLDVVERSPHRPILQAGLPANASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNGSKYGPDGLPYLKVLKAAGVNTTDKEIEVLYIRNVTFEDAGEYTCLAGNSIGISFHSAWLTVLPAPGREKEITASPDYLEIAIYCIGVFLIACMVVTVILCRMKNTTKKPDFSSQPAVHKLTKRIPLRRQVTVSAESSSSMNSNTPLVRITTRLSSTADTPMLAGVSEYELPEDPKWEFPRDKLTLGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVKMLKDDATEKDLSDLVSEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLRARRPPGMEYSYDINRVPEEQMTFKDLVSCTYQLARGMEYLASQKCIHRDLAARNVLVTENNVMKIADFGLARDINNIDYYKKTTNGRLPVKWMAPEALFDRVYTHQSDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLLKEGHRMDKPANCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRILTLTTNEEYLDLSQPLEQYSPSYPDTRSSCSSGDDSVFSPDPMPYEPCLPQYPHINGSVKT heparin-fgf2-2 1886869 N/A 1886872 unspecified role unspecified role prey prey N/A 1886873 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false 4C-1 CHEBI:90931 causes no change to the binding of CHEBI:28304 to P09038 fgfr1-fgf2-1 1886869 N/A 1886874 unspecified role unspecified role bait bait ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 1886875 unspecified role unspecified role prey prey direct interaction direct interaction false false false 4C-1i fgfr1-fgf2-2 1886869 N/A 1886876 inhibitor inhibitor neutral component neutral component N/A 1886874 unspecified role unspecified role bait bait ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c_terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 1886872 unspecified role unspecified role prey prey direct interaction direct interaction false false false 4C-1ii NSC12 - CHEBI:90931 ID50 ~30 μM CHEBI:90931 decreases the binding of p11362 to P09038 fgf2-nsc12-1 1886869 N/A 1886872 unspecified role unspecified role bait bait Stoichiometry: 1.0 N/A 1886876 unspecified role unspecified role prey prey Stoichiometry: 1.0 direct interaction direct interaction false false false 1886869 4D fgf3-nsc12-1 1886869 N/A 1886877 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1886876 unspecified role unspecified role prey prey direct interaction direct interaction false false false 1886869 4F-1 fgf4-nsc12-1 1886869 N/A 1886878 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1886876 unspecified role unspecified role prey prey direct interaction direct interaction false false false 1886869 4F-2 fgf6-nsc12-1 1886869 N/A 1886879 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1886876 unspecified role unspecified role prey prey direct interaction direct interaction false false false 1886869 4F-3 fgf8-nsc12-1 1886869 N/A 1886880 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1886876 unspecified role unspecified role prey prey direct interaction direct interaction false false false 1886869 4F-4 fgf16-nsc12-1 1886869 N/A 1886881 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1886876 unspecified role unspecified role prey prey direct interaction direct interaction false false false 1886869 4F-5 fgf18-nsc12-1 1886869 N/A 1886882 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1886876 unspecified role unspecified role prey prey direct interaction direct interaction false false false 1886869 4F-6 fgf20-nsc12-1 1886869 N/A 1886883 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1886876 unspecified role unspecified role prey prey direct interaction direct interaction false false false 1886869 4F-7 fgf20-nsc12-2 1886869 N/A 1886884 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 1886876 unspecified role unspecified role prey prey direct interaction direct interaction false false false 1886869 4F-8 fgf2-fgfr2-4 1886870 N/A 1886872 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1886885 unspecified role unspecified role neutral component neutral component d2_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) 1886940 1886938 direct interaction direct interaction false false false Supp 3B fgf2-fgfr2-7 1886870 N/A 1886876 inhibitor inhibitor neutral component neutral component N/A 1886872 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 1886885 unspecified role unspecified role neutral component neutral component d2_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) 1886947 1886945 direct interaction direct interaction false false false Supp 3Bi NSC12 (CHEBI:90931) CHEBI:90931 decreases the binding between P09038 and P21802 fgf2-nsc12-2 1886871 N/A 1886876 unspecified role unspecified role prey prey N/A 1886872 unspecified role unspecified role bait bait direct interaction direct interaction false false false 1886871 4Dii No nanomechanical effect is seen when NSC12 is run onto a urea-denatured FGF2 chip.