IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ meyer-2015-1 human-set2 SET2 - Human acute leukemia cell line (megakaryoblastic) set2 SET2 Human acute leukemia cell line (megakaryoblastic) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Meyer SC., Keller MD., Chiu S., Koppikar P., Guryanova OA., Rapaport F., Xu K., Manova K., Pankov D., O'Reilly RJ., Kleppe M., McKenney AS., Shih AH., Shank K., Ahn J., Papalexi E., Spitzer B., Socci N., Viale A., Mandon E., Ebel N., Andraos R., Rubert J., Dammassa E., Romanet V., Dölemeyer A., Zender M., Heinlein M., Rampal R., Weinberg RS., Hoffman R., Sellers WR., Hofmann F., Murakami M., Baffert F., Gaul C., Radimerski T., Levine RL. Cancer - Interactions investigated in the context of cancer Cells were grown in the presence or absence of type I JAK inhibitors CYT387 or BMS911543, or SAR302503 or Ruxolitinib. In the presence of inhibitors cells acquire an adaptive form of drug resistance and are here called "inhibitor persistent cells". thomas.radimerski@novartis.com,leviner@mskcc.org Cancer cell 2015 Only protein-protein interactions imex curation imex curation meyer-2015-2 human-set2 SET2 - Human acute leukemia cell line (megakaryoblastic) set2 SET2 Human acute leukemia cell line (megakaryoblastic) confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Meyer SC., Keller MD., Chiu S., Koppikar P., Guryanova OA., Rapaport F., Xu K., Manova K., Pankov D., O'Reilly RJ., Kleppe M., McKenney AS., Shih AH., Shank K., Ahn J., Papalexi E., Spitzer B., Socci N., Viale A., Mandon E., Ebel N., Andraos R., Rubert J., Dammassa E., Romanet V., Dölemeyer A., Zender M., Heinlein M., Rampal R., Weinberg RS., Hoffman R., Sellers WR., Hofmann F., Murakami M., Baffert F., Gaul C., Radimerski T., Levine RL. Cancer - Interactions investigated in the context of cancer Cells were grown in the presence and absence of type I JAK inhibitors CYT387 or ruxolitinib. In the presence of inhibitors cells acquire an adaptive form of drug resistance and are here called "inhibitor persistent cells". thomas.radimerski@novartis.com,leviner@mskcc.org Cancer cell 2015 Only protein-protein interactions imex curation imex curation meyer-2015-3 human-gamma2a Human fibroblasts lacking JAK2 Human gamma2a Human fibroblasts lacking JAK2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Meyer SC., Keller MD., Chiu S., Koppikar P., Guryanova OA., Rapaport F., Xu K., Manova K., Pankov D., O'Reilly RJ., Kleppe M., McKenney AS., Shih AH., Shank K., Ahn J., Papalexi E., Spitzer B., Socci N., Viale A., Mandon E., Ebel N., Andraos R., Rubert J., Dammassa E., Romanet V., Dölemeyer A., Zender M., Heinlein M., Rampal R., Weinberg RS., Hoffman R., Sellers WR., Hofmann F., Murakami M., Baffert F., Gaul C., Radimerski T., Levine RL. Cancer - Interactions investigated in the context of cancer thomas.radimerski@novartis.com,leviner@mskcc.org Cancer cell 2015 Only protein-protein interactions imex curation imex curation jak2_human Tyrosine-protein kinase JAK2 Janus kinase 2 JAK2 protein protein human Homo sapiens Human MGMACLTMTEMEGTSTSSIYQNGDISGNANSMKQIDPVLQVYLYHSLGKSEADYLTFPSGEYVAEEICIAASKACGITPVYHNMFALMSETERIWYPPNHVFHIDESTRHNVLYRIRFYFPRWYCSGSNRAYRHGISRGAEAPLLDDFVMSYLFAQWRHDFVHGWIKVPVTHETQEECLGMAVLDMMRIAKENDQTPLAIYNSISYKTFLPKCIRAKIQDYHILTRKRIRYRFRRFIQQFSQCKATARNLKLKYLINLETLQSAFYTEKFEVKEPGSGPSGEEIFATIIITGNGGIQWSRGKHKESETLTEQDLQLYCDFPNIIDVSIKQANQEGSNESRVVTIHKQDGKNLEIELSSLREALSFVSLIDGYYRLTADAHHYLCKEVAPPAVLENIQSNCHGPISMDFAISKLKKAGNQTGLYVLRCSPKDFNKYFLTFAVERENVIEYKHCLITKNENEEYNLSGTKKNFSSLKDLLNCYQMETVRSDNIIFQFTKCCPPKPKDKSNLLVFRTNGVSDVPTSPTLQRPTHMNQMVFHKIRNEDLIFNESLGQGTFTKIFKGVRREVGDYGQLHETEVLLKVLDKAHRNYSESFFEAASMMSKLSHKHLVLNYGVCVCGDENILVQEFVKFGSLDTYLKKNKNCINILWKLEVAKQLAWAMHFLEENTLIHGNVCAKNILLIREEDRKTGNPPFIKLSDPGISITVLPKDILQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDLALRVDQIRDNMAG jak1_human Tyrosine-protein kinase JAK1 JAK1 JAK1A JAK1B Janus kinase 1 protein protein human Homo sapiens Human MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK tyk2_human Non-receptor tyrosine-protein kinase TYK2 TYK2 protein protein human Homo sapiens Human MPLRHWGMARGSKPVGDGAQPMAAMGGLKVLLHWAGPGGGEPWVTFSESSLTAEEVCIHIAHKVGITPPCFNLFALFDAQAQVWLPPNHILEIPRDASLMLYFRIRFYFRNWHGMNPREPAVYRCGPPGTEASSDQTAQGMQLLDPASFEYLFEQGKHEFVNDVASLWELSTEEEIHHFKNESLGMAFLHLCHLALRHGIPLEEVAKKTSFKDCIPRSFRRHIRQHSALTRLRLRNVFRRFLRDFQPGRLSQQMVMVKYLATLERLAPRFGTERVPVCHLRLLAQAEGEPCYIRDSGVAPTDPGPESAAGPPTHEVLVTGTGGIQWWPVEEEVNKEEGSSGSSGRNPQASLFGKKAKAHKAVGQPADRPREPLWAYFCDFRDITHVVLKEHCVSIHRQDNKCLELSLPSRAAALSFVSLVDGYFRLTADSSHYLCHEVAPPRLVMSIRDGIHGPLLEPFVQAKLRPEDGLYLIHWSTSHPYRLILTVAQRSQAPDGMQSLRLRKFPIEQQDGAFVLEGWGRSFPSVRELGAALQGCLLRAGDDCFSLRRCCLPQPGETSNLIIMRGARASPRTLNLSQLSFHRVDQKEITQLSHLGQGTRTNVYEGRLRVEGSGDPEEGKMDDEDPLVPGRDRGQELRVVLKVLDPSHHDIALAFYETASLMSQVSHTHLAFVHGVCVRGPENIMVTEYVEHGPLDVWLRRERGHVPMAWKMVVAQQLASALSYLENKNLVHGNVCGRNILLARLGLAEGTSPFIKLSDPGVGLGALSREERVERIPWLAPECLPGGANSLSTAMDKWGFGATLLEICFDGEAPLQSRSPSEKEHFYQRQHRLPEPSCPQLATLTSQCLTYEPTQRPSFRTILRDLTRLQPHNLADVLTVNPDSPASDPTVFHKRYLKKIRDLGEGHFGKVSLYCYDPTNDGTGEMVAVKALKADCGPQHRSGWKQEIDILRTLYHEHIIKYKGCCEDQGEKSLQLVMEYVPLGSLRDYLPRHSIGLAQLLLFAQQICEGMAYLHAQHYIHRDLAARNVLLDNDRLVKIGDFGLAKAVPEGHEYYRVREDGDSPVFWYAPECLKEYKFYYASDVWSFGVTLYELLTHCDSSQSPPTKFLELIGIAQGQMTVLRLTELLERGERLPRPDKCPCEVYHLMKNCWETEASFRPTFENLIPILKTVHEKYQGQAPSVFSVC jak1-jak2-1 1886503 N/A 1886506 unspecified role unspecified role prey prey phosres phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1886507 unspecified role unspecified role bait bait physical association physical association false false false Supp 1 J/K, 3 Increased association of phosphorylated JAK2 was observed in "inhibitor persistent cells". CHZ868 (CHEBI:90828) a a Type II JAK2 Inhibitor;a reduced amount of phosphorylated JAK2 was bound to JAK1 or TYK2 in persistent cell cultures. tyk2-jak2-1 1886503 N/A 1886506 unspecified role unspecified role prey prey phosres phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1886508 unspecified role unspecified role bait bait physical association physical association false false false Supp 1 J/K, 3 Increased association of phosphorylated JAK2 and JAK1 was observed in "inhibitor persistent cells". CHZ868 (CHEBI:90828) a a Type II JAK2 Inhibitor;a reduced amount of phosphorylated JAK2 was bound to JAK1 or TYK2 in persistent cell cultures. jak1-jak2-3 1886503 N/A 1886506 unspecified role unspecified role prey prey tyr221 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false tyr1007 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false tyr1008 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false N/A 1886507 unspecified role unspecified role bait bait physical association physical association false false false 3 A/B/D Increased association of phosphorylated JAK2 was observed in "inhibitor persistent cells". CHZ868 inhibited phosphorylation at Y221 (as well as Y1007/8) in type I JAK inhibitor persistent cells. CHZ868 (CHEBI:90828) a a Type II JAK2 Inhibitor;a reduced amount of phosphorylated JAK2 was bound to JAK1 or TYK2 in persistent cell cultures. tyk2-jak2-2 1886503 N/A 1886506 unspecified role unspecified role prey prey tyr1007 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false tyr1008 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false N/A 1886508 unspecified role unspecified role bait bait physical association physical association false false false 3 A/B/D Increased association of phosphorylated JAK2 and JAK1 was observed in "inhibitor persistent cells". CHZ868 inhibited phosphorylation at Y221 (as well as Y1007/8) in type I JAK inhibitor persistent cells. CHZ868 (CHEBI:90828) a a Type II JAK2 Inhibitor;a reduced amount of phosphorylated JAK2 was bound to JAK1 or TYK2 in persistent cell cultures. jak1-jak2-2 1886504 N/A 1886506 unspecified role unspecified role neutral component neutral component N/A 1886507 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Supp 1 L/N There is an increase in colocalisation in the "persistent" cells. jak1-jak2-4 1886505 N/A 1886506 unspecified role unspecified role prey prey tyr1007 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false tyr1008 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false O60674:p.Lys882Glu mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 1886507 unspecified role unspecified role bait bait P23458:p.Val658Phe mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3 C CHZ868 (CHEBI:90828) a a Type II JAK2 Inhibitor. CHZ868 reduced JAK1-mediated Y1007/Y1008 phosphorylation of JAK2 K882E (kinase dead)