IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ zhang-2015-6 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr Cancer cell 2015 Only protein-protein interactions imex curation imex curation zhang-2015-7 human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr Cancer cell 2015 Only protein-protein interactions imex curation imex curation zhang-2015-8 human-hmle Homo sapiens hmle Human immortalized mammary epithelial cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr Cancer cell 2015 Only protein-protein interactions imex curation imex curation zhang-2015-10 mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr Cancer cell 2015 Only protein-protein interactions imex curation imex curation zhang-2015-11 human-hmle Homo sapiens hmle Human immortalized mammary epithelial cell line ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr Cancer cell 2015 Only protein-protein interactions imex curation imex curation zhang-2015-5 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr human placental cDNA library Cancer cell 2015 Only protein-protein interactions imex curation imex curation zhang-2015-9 chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr Cancer cell 2015 Only protein-protein interactions imex curation imex curation zhang-2015-12 human-mammary gland human mammary gland Human mammary_gland mammary gland fluorescence imaging fluorescence microscopy fluorescence imaging immunohistochemistry immunohistochemistry Zhang MZ., Ferrigno O., Wang Z., Ohnishi M., Prunier C., Levy L., Razzaque M., Horne WC., Romero D., Tzivion G., Colland F., Baron R., Atfi A. Cancer - Interactions investigated in the context of cancer Origin of Flag-TGIF has been entered as Human:- "Flag-TGIF were a gift from Dr. J. Massagué ", according to the reference Pessah et al., 2002. Addgene sell a plasmid ( pCMV5 Flag TGIF (Plasmid #14047) ) donated by Joan Massague. Myc-Axin1 has been entered as of Murine origin, as being the most likely Addgene plasmid used, as Addgene sell both Mouse and Frog Myc-Axin 1. Interactions involving Beta-Catenin tagged constructs have not been entered as the origins of the constructs are not stated; - they "were kindly provided by Dr. Yang". Similarly results from a Pull Down (Supp. fig 2C) have not been entered as the origins for GST-CRM1 are not stated. The authors have not replied to a request for information. azeddine.atfi@inserm.fr Cancer cell 2015 Only protein-protein interactions imex curation imex curation axin2_mouse Axin-2 Axin2 Axis inhibition protein 2 Conductin Axin-like protein protein protein mouse Mus musculus Mouse MSSAVLVTLLPDPSSSFREDAPRPPVPGEEGETPPCQPSVGKVQSTKPMPVSSNARRNEDGLGEPEGRASPDSPLTRWTKSLHSLLGDQDGAYLFRTFLEREKCVDTLDFWFACNGFRQMNLKDTKTLRVAKAIYKRYIENNSVVSKQLKPATKTYIRDGIKKQQIGSVMFDQAQTEIQAVMEENAYQVFLTSDIYLEYVRSGGENTAYMSNGGLGSLKVLCGYLPTLNEEEEWTCADLKCKLSPTVVGLSSKTLRATASVRSTETAENGFRSFKRSDPVNPYHVGSGYVFAPATSANDSELSSDALTDDSMSMTDSSVDGVPPYRMGSKKQLQREMHRSVKANGQVSLPHFPRTHRLPKEMTPVEPAAFAAELISRLEKLKLELESRHSLEERLQQIREDEEKEGSEQALSSRDGAPVQHPLALLPSGSYEEDPQTILDDHLSRVLKTPGCQSPGVGRYSPRSRSPDHHHQHHHHQQCHTLLPTGGKLPPVAACPLLGGKSFLTKQTTKHVHHHYIHHHAVPKTKEEIEAEATQRVRCLCPGGTDYYCYSKCKSHPKAPEPLPGEQFCGSRGGTLPKRNAKGTEPGLALSARDGGMSSAAGAPQLPGEEGDRSQDVWQWMLESERQSKSKPHSAQSIRKSYPLESACAAPGERVSRHHLLGASGHSRSVARAHPFTQDPAMPPLTPPNTLAQLEEACRRLAEVSKPQKQRCCVASQQRDRNHSAAGQAGASPFANPSLAPEDHKEPKKLASVHALQASELVVTYFFCGEEIPYRRMLKAQSLTLGHFKEQLSKKGNYRYYFKKASDEFACGAVFEEIWDDETVLPMYEGRILGKVERID mint homomint Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B tgif1_human Homeobox protein TGIF1 5'-TG-3'-interacting factor 1 TGIF1 TGIF protein protein human Homo sapiens Human MVLAQSRVSAGVGSPHCSGSGGGGSDSFPWPASHPGNPQCSFSTAFLASPRLSRGTLAYLPPAPWSSLATPSALLGSSCAPPPPPARCPQPRALSPELGTKAGPRRPHRWELPRSPSQGAQGPAPRRRLLETMKGIVAASGSETEDEDSMDIPLDLSSSAGSGKRRRRGNLPKESVQILRDWLYEHRYNAYPSEQEKALLSQQTHLSTLQVCNWFINARRRLLPDMLRKDGKDPNQFTISRRGAKISETSSVESVMGIKNFMPALEETPFHSCTAGPNPTLGRPLSPKPSSPGSVLARPSVICHTTVTALKDVPFSLCQSVGVGQNTDIQQIAAKNFTDTSLMYPEDTCKSGPSTNTQSGLFNTPPPTPPDLNQDFSGFQLLVDVALKRAAEMELQAKLTA axin1_mouse Axin-1 Axin1 Axin Fu Axis inhibition protein 1 Protein Fused protein protein mouse Mus musculus Mouse MNVQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDSRPVNHSFCSGKGTSIKSETSTATPRRSDLDLGYEPEGSASPTPPYLRWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACSGFRKLEPCDSNEEKRLKLARAIYRKYILDSNGIVSRQTKPATKSFIKDCVMKQQIDPAMFDQAQTEIQSTMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGMSGYLPTLNEDEEWKCDQDADEDDGRDPLPPSRLTQKLLLETAAPRAPSSRRYNEGRELRYGSWREPVNPYYVNSGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESIQVNGRVPLPHIPRTYRMPKEIRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGEMPSGPMASHKLPSVPAWHHFPPRYVDMGCSGLRDAHEENPESILDEHVQRVMRTPGCQSPGPGHRSPDSGHVAKTAVLGGTASGHGKHVPKLGLKLDTAGLHHHRHVHHHVHHNSARPKEQMEAEVARRVQSSFSWGPETHGHAKPRSYSENAGTTLSAGDLAFGGKTSAPSKRNTKKAESGKNANAEVPSTTEDAEKNQKIMQWIIEGEKEISRHRKAGHGSSGLRKQQAHESSRPLSIERPGAVHPWVSAQLRNSVQPSHLFIQDPTMPPNPAPNPLTQLEEARRRLEEEEKRANKLPSKQRYVQAVMQRGRTCVRPACAPVLSVVPAVSDLELSETETKSQRKAGGGSAPPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD axin2_human Axin-2 AXIN2 Axin-like protein Axis inhibition protein 2 Conductin protein protein human Homo sapiens Human MSSAMLVTCLPDPSSSFREDAPRPPVPGEEGETPPCQPGVGKGQVTKPMSVSSNTRRNEDGLGEPEGRASPDSPLTRWTKSLHSLLGDQDGAYLFRTFLEREKCVDTLDFWFACNGFRQMNLKDTKTLRVAKAIYKRYIENNSIVSKQLKPATKTYIRDGIKKQQIDSIMFDQAQTEIQSVMEENAYQMFLTSDIYLEYVRSGGENTAYMSNGGLGSLKVVCGYLPTLNEEEEWTCADFKCKLSPTVVGLSSKTLRATASVRSTETVDSGYRSFKRSDPVNPYHIGSGYVFAPATSANDSEISSDALTDDSMSMTDSSVDGIPPYRVGSKKQLQREMHRSVKANGQVSLPHFPRTHRLPKEMTPVEPATFAAELISRLEKLKLELESRHSLEERLQQIREDEEREGSELTLNSREGAPTQHPLSLLPSGSYEEDPQTILDDHLSRVLKTPGCQSPGVGRYSPRSRSPDHHHHHHSQYHSLLPPGGKLPPAAASPGACPLLGGKGFVTKQTTKHVHHHYIHHHAVPKTKEEIEAEATQRVHCFCPGGSEYYCYSKCKSHSKAPETMPSEQFGGSRGSTLPKRNGKGTEPGLALPAREGGAPGGAGALQLPREEGDRSQDVWQWMLESERQSKPKPHSAQSTKKAYPLESARSSPGERASRHHLWGGNSGHPRTTPRAHLFTQDPAMPPLTPPNTLAQLEEACRRLAEVSKPPKQRCCVASQQRDRNHSATVQTGATPFSNPSLAPEDHKEPKKLAGVHALQASELVVTYFFCGEEIPYRRMLKAQSLTLGHFKEQLSKKGNYRYYFKKASDEFACGAVFEEIWEDETVLPMYEGRILGKVERID axin1_human Axin-1 Axis inhibition protein 1 AXIN1 AXIN protein protein human Homo sapiens Human MNIQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDPRPASYSFCSGKGVGIKGETSTATPRRSDLDLGYEPEGSASPTPPYLKWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACTGFRKLEPCDSNEEKRLKLARAIYRKYILDNNGIVSRQTKPATKSFIKGCIMKQLIDPAMFDQAQTEIQATMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGISGYLPTLNEDEEWKCDQDMDEDDGRDAAPPGRLPQKLLLETAAPRVSSSRRYSEGREFRYGSWREPVNPYYVNAGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESVQVNGRVPLPHIPRTYRVPKEVRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGDPSSGPPGPCHKLPPAPAWHHFPPRCVDMGCAGLRDAHEENPESILDEHVQRVLRTPGRQSPGPGHRSPDSGHVAKMPVALGGAASGHGKHVPKSGAKLDAAGLHHHRHVHHHVHHSTARPKEQVEAEATRRAQSSFAWGLEPHSHGARSRGYSESVGAAPNASDGLAHSGKVGVACKRNAKKAESGKSASTEVPGASEDAEKNQKIMQWIIEGEKEISRHRRTGHGSSGTRKPQPHENSRPLSLEHPWAGPQLRTSVQPSHLFIQDPTMPPHPAPNPLTQLEEARRRLEEEEKRASRAPSKQRYVQEVMRRGRACVRPACAPVLHVVPAVSDMELSETETRSQRKVGGGSAQPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD fbw1a_human F-box/WD repeat-containing protein 1A BTRC BTRCP FBW1A FBXW1A F-box and WD repeats protein beta-TrCP E3RSIkappaB pIkappaBalpha-E3 receptor subunit Epididymis tissue protein Li 2a protein protein human Homo sapiens Human MDPAEAVLQEKALKFMCSMPRSLWLGCSSLADSMPSLRCLYNPGTGALTAFQNSSEREDCNNGEPPRKIIPEKNSLRQTYNSCARLCLNQETVCLASTAMKTENCVAKTKLANGTSSMIVPKQRKLSASYEKEKELCVKYFEQWSESDQVEFVEHLISQMCHYQHGHINSYLKPMLQRDFITALPARGLDHIAENILSYLDAKSLCAAELVCKEWYRVTSDGMLWKKLIERMVRTDSLWRGLAERRGWGQYLFKNKPPDGNAPPNSFYRALYPKIIQDIETIESNWRCGRHSLQRIHCRSETSKGVYCLQYDDQKIVSGLRDNTIKIWDKNTLECKRILTGHTGSVLCLQYDERVIITGSSDSTVRVWDVNTGEMLNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMASPTDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWNTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLVAALDPRAPAGTLCLRTLVEHSGRVFRLQFDEFQIVSSSHDDTILIWDFLNDPAAQAEPPRSPSRTYTYISR ctnb1_human Catenin beta-1 Beta-catenin OK/SW-cl.35 PRO2286 CTNNB1 CTNNB protein protein human Homo sapiens Human MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL cadh1_mouse Cadherin-1 Epithelial cadherin Uvomorulin ARC-1 Cdh1 protein protein mouse Mus musculus Mouse MGARCRSFSALLLLLQVSSWLCQELEPESCSPGFSSEVYTFPVPERHLERGHVLGRVRFEGCTGRPRTAFFSEDSRFKVATDGTITVKRHLKLHKLETSFLVRARDSSHRELSTKVTLKSMGHHHHRHHHRDPASESNPELLMFPSVYPGLRRQKRDWVIPPISCPENEKGEFPKNLVQIKSNRDKETKVFYSITGQGADKPPVGVFIIERETGWLKVTQPLDREAIAKYILYSHAVSSNGEAVEDPMEIVITVTDQNDNRPEFTQEVFEGSVAEGAVPGTSVMKVSATDADDDVNTYNAAIAYTIVSQDPELPHKNMFTVNRDTGVISVLTSGLDRESYPTYTLVVQAADLQGEGLSTTAKAVITVKDINDNAPVFNPSTYQGQVPENEVNARIATLKVTDDDAPNTPAWKAVYTVVNDPDQQFVVVTDPTTNDGILKTAKGLDFEAKQQYILHVRVENEEPFEGSLVPSTATVTVDVVDVNEAPIFMPAERRVEVPEDFGVGQEITSYTAREPDTFMDQKITYRIWRDTANWLEINPETGAIFTRAEMDREDAEHVKNSTYVALIIATDDGSPIATGTGTLLLVLLDVNDNAPIPEPRNMQFCQRNPQPHIITILDPDLPPNTSPFTAELTHGASVNWTIEYNDAAQESLILQPRKDLEIGEYKIHLKLADNQNKDQVTTLDVHVCDCEGTVNNCMKAGIVAAGLQVPAILGILGGILALLILILLLLLFLRRRTVVKEPLLPPDDDTRDNVYYYDEEGGGEEDQDFDLSQLHRGLDARPEVTRNDVAPTLMSVPQYRPRPANPDEIGNFIDENLKAADSDPTAPPYDSLLVFDYEGSGSEAASLSSLNSSESDQDQDYDYLNEWGNRFKKLADMYGGGEDD ctnb1_mouse Catenin beta-1 Beta-catenin Ctnnb1 Catnb protein protein mouse Mus musculus Mouse MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEALGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL xpo1_mouse Exportin-1 Xpo1 Crm1 Chromosome region maintenance 1 protein homolog protein protein mouse Mus musculus Mouse MPAIMTMLADHAARQLLDFSQKLDINLLDNVVNCLYHGEGAQQRMAQEVLTHLKEHPDAWTRVDTILEFSQNMNTKYYGLQILENVIKTRWKILPRNQCEGIKKYVVGLIIKTSSDPTCVEKEKVYIGKLNMILVQILKQEWPKHWPTFISDIVGASRTSESLCQNNMVILKLLSEEVFDFSSGQITQVKAKHLKDSMCNEFSQIFQLCQFVMENSQNAPLVHATLETLLRFLNWIPLGYIFETKLISTLIYKFLNVPMFRNVSLKCLTEIAGVSVSQYEEQFETLFTLTMMQLKQMLPLNTNIRLAYSNGKDDEQNFIQNLSLFLCTFLKEHGQLLEKRLNLREALMEALHYMLLVSEVEETEIFKICLEYWNHLAAELYRESPFSTSASPLLSGSQHFDIPPRRQLYLTVLSKVRLLMVSRMAKPEEVLVVENDQGEVVREFMKDTDSINLYKNMRETLVYLTHLDYVDTEIIMTKKLQNQVNGTEWSWKNLNTLCWAIGSISGAMHEEDEKRFLVTVIKDLLGLCEQKRGKDNKAIIASNIMYIVGQYPRFLRAHWKFLKTVVNKLFEFMHETHDGVQDMACDTFIKIAQKCRRHFVQVQVGEVMPFIDEILNNINTIICDLQPQQVHTFYEAVGYMIGAQTDQTVQEHLIEKYMLLPNQVWDSIIQQATKNVDILKDPETVKQLGSILKTNVRACKAVGHPFVIQLGRIYLDMLNVYKCLSENISAAIQANGEMVTKQPLIRSMRTVKRETLKLISGWVSRSNDPQMVAENFVPPLLDAVLIDYQRNVPAAREPEVLSTMAIIVNKLGGHITAEIPQIFDAVFECTLNMINKDFEEYPEHRTNFFLLLQAVNSHCFPAFLAIPPAQFKLVLDSIIWAFKHTMRNVADTGLQILFTLLQNVAQEEAAAQSFYQTYFCDILQHIFSVVTDTSHTAGLTMHASILAYMFNLVEEGKISTPLNPGNPVNNQMFIQDYVANLLKSAFPHLQDAQVKLFVTGLFSLNQDIPAFKEHLRDFLVQIKEFAGEDTSDLFLEERETALRQAQEEKHKLQMSVPGILNPHEIPEEMCD fbw1a_mouse F-box/WD repeat-containing protein 1A Btrc Fbw1 Fbxw1 Fwd1 Kiaa4123 Beta-TrCP protein E3RS-IkappaB Beta-transducin repeat-containing protein E3RSIkappaB F-box and WD repeats protein beta-TrCP HOS Ubiquitin ligase FWD1 pIkappaB-E3 receptor subunit protein protein mouse Mus musculus Mouse MDPAEAVLQEKALKFMCSMPRSLWLGCSSLADSMPSLRCLYNPGTGALTAFQNSSEREDCNNGEPPRKIIPEKNSLRQTYNSCARLCINQETVCLTSTAMKTENCVAKAKLANGTSSMIVPKQRKLSASYEKEKELCVKYFEQWSESDQVEFVEHLISQMCHYQHGHINSYLKPMLQRDFITALPARGLDHIAENILSYLDAKSLCAAELVCKEWYRVTSDGMLWKKLIERMVRTDSLWRGLAERRGWGQYLFKNKPPDENAPPNSFYRALYPKIIQDIETIESNWRCGRHSLQRIHCRSETSKGVYCLQYDDQKIVSGLRDNTIKIWDKSTLECKRILTGHTGSVLCLQYDERVIITGSSDSTVRVWDVNAGEMLNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMASPTDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWNTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLMAALDPRAPAGTLCLRTLVEHSGRVFRLQFDEFQIVSSSHDDTILIWDFLNDPAAHAEPPRSPSRTYTYISR tgif1_human_gene Homeobox protein TGIF1 TGIF1 TGIF gene gene human Homo sapiens Human tf7l2_human Transcription factor 7-like 2 TCF7L2 TCF4 HMG box transcription factor 4 T-cell-specific transcription factor 4 protein protein human Homo sapiens Human MPQLNGGGGDDLGANDELISFKDEGEQEEKSSENSSAERDLADVKSSLVNESETNQNSSSDSEAERRPPPRSESFRDKSRESLEEAAKRQDGGLFKGPPYPGYPFIMIPDLTSPYLPNGSLSPTARTLHFQSGSTHYSAYKTIEHQIAVQYLQMKWPLLDVQAGSLQSRQALKDARSPSPAHIVSNKVPVVQHPHHVHPLTPLITYSNEHFTPGNPPPHLPADVDPKTGIPRPPHPPDISPYYPLSPGTVGQIPHPLGWLVPQQGQPVYPITTGGFRHPYPTALTVNASMSRFPPHMVPPHHTLHTTGIPHPAIVTPTVKQESSQSDVGSLHSSKHQDSKKEEEKKKPHIKKPLNAFMLYMKEMRAKVVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKKRKRDKQPGETNEHSECFLNPCLSLPPITDLSAPKKCRARFGLDQQNNWCGPCRRKKKCVRYIQGEGSCLSPPSSDGSLLDSPPPSPNLLGSPPRDAKSQTEQTQPLSLSLKPDPLAHLSMMPPPPALLLAEATHKASALCPNGALDLPPAALQPAAPSSSIAQPSTSSLHSHSSLAGTQPQPLSLVTKSLE tgif1-axin2-1 1808139 N/A 1808147 unspecified role unspecified role prey prey N/A myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1808148 unspecified role unspecified role bait bait N/A flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 1A, Supp 1A Wnt3a tgif1-axin2-3 1808139 N/A 1808149 unspecified role unspecified role bait bait N/A myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1808148 unspecified role unspecified role prey prey N/A flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 1D Wnt3a tgif1-axin2-5 1808139 N/A 1808147 unspecified role unspecified role prey prey N/A flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 1808148 unspecified role unspecified role bait bait N/A ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Supp 1B/C Wnt3a axin1-tgif1-1 1808139 N/A 1808149 unspecified role unspecified role prey prey N/A myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1808148 unspecified role unspecified role bait bait N/A flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Supp 1D Wnt3a tgif1-axin1-1 1808140 N/A 1808150 unspecified role unspecified role prey prey N/A 1808151 unspecified role unspecified role prey prey N/A 1808148 unspecified role unspecified role bait bait association association false false false 1E Wnt3a btrc-1 1808140 N/A 1808152 unspecified role unspecified role bait bait N/A 1808153 unspecified role unspecified role prey prey physical association physical association false false false 3D Induction of TGIF expression resulted in decreased interaction of beta-catenin with beta-TRCP. tgif1-axin1-4 1808140 N/A 1808151 unspecified role unspecified role bait bait N/A 1808148 unspecified role unspecified role prey prey physical association physical association false false false Supp 1E Wnt3a axin1-axin2-1 1808140 N/A 1808150 unspecified role unspecified role bait bait N/A 1808148 unspecified role unspecified role prey prey physical association physical association false false false Supp 1Ei Wnt3a tgif1-axin1-2 1808141 N/A 1808150 unspecified role unspecified role prey prey N/A 1808151 unspecified role unspecified role prey prey N/A 1808148 unspecified role unspecified role bait bait association association false false false 1 E/F Wnt3a axin2-tgif1-1 1808141 N/A 1808148 unspecified role unspecified role prey prey N/A 1808150 unspecified role unspecified role bait bait physical association physical association false false false Supp 1Ei Wnt3a axin1-tgif1-2 1808141 N/A 1808151 unspecified role unspecified role bait bait N/A 1808148 unspecified role unspecified role prey prey physical association physical association false false false Supp 1E Wnt3a tgif1-axin1-3 1808142 N/A 1808147 unspecified role unspecified role prey prey N/A 1808154 unspecified role unspecified role prey prey N/A 1808155 unspecified role unspecified role bait bait N/A 1808149 unspecified role unspecified role prey prey association association false false false 2D TGIF deficiency increases the interaction of Axin1 and 2 with beta-catenin. ctnnb1-axin1-8 1808142 N/A 1808147 unspecified role unspecified role prey prey N/A 1808156 unspecified role unspecified role bait bait N/A 1808149 unspecified role unspecified role prey prey association association false false false 2E TGIF deficiency increases the interaction of Axin1 and 2 with beta-catenin. ctnnb1-axin1-9 1808142 N/A 1808157 unspecified role unspecified role prey prey N/A 1808155 unspecified role unspecified role bait bait putative phosphorylated residue opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3A TGIF deficiency increases the interaction of Axin1 and 2 with beta-catenin. ctnnb1-1 1808143 N/A 1808153 unspecified role unspecified role bait bait N/A 1808158 unspecified role unspecified role prey prey N/A sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5B Wnt3a P35222 increases expression at protein level of ENSG00000177426 P35222 increases expression at RNA level of ENSG00000177426 tcf7l2-tgif1-1 1808143 N/A 1808159 unspecified role unspecified role bait bait N/A 1808158 unspecified role unspecified role prey prey N/A sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5Bi Wnt3a Q9NQB0 increases expression at RNA level of ENSG00000177426 Q9NQB0 increases expression at protein level of ENSG00000177426 tgif1-axin2-2 1808144 N/A 1808150 unspecified role unspecified role prey prey N/A 1808148 unspecified role unspecified role bait bait N/A sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false no figure tgif1-axin2-4 1808145 N/A 1808147 unspecified role unspecified role neutral component neutral component N/A flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1808148 unspecified role unspecified role neutral component neutral component N/A gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 2A ctnnb1-tgif1-1 1808146 N/A 1808153 unspecified role unspecified role neutral component neutral component N/A 1808148 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false S3C the co-expression of TGIF and beta-catenin is mainly confined to the whole epithelial duct structure.