IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ johnston-2014-1 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Johnston RJ., Comps-Agrar L., Hackney J., Yu X., Huseni M., Yang Y., Park S., Javinal V., Chiu H., Irving B., Eaton DL., Grogan JL. Cancer - Interactions investigated in the context of cancer Figure 7D:-Cells were transfected with N-terminus SNAP-tagged (ST) CD226. The SNAP tags were then labelled with either donor-conjugated benzylguanine SNAP-Lumi-4Tb or acceptor-conjugated benzyl-guanine SNAP-A647. Figure 7F:- Cells were transfected with N-terminus SNAP-tagged (ST) CD226 or HA-TIGIT. The SNAP tag was then labelled with acceptor conjugated benzyl-guanine SNAP-A647 and the HA tag was labelled with donor-conjugated anti-HA Lumi-4Tb. grogan.jane@gene.com Cancer cell 2014 Only protein-protein interactions imex curation imex curation johnston-2014-2 chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Johnston RJ., Comps-Agrar L., Hackney J., Yu X., Huseni M., Yang Y., Park S., Javinal V., Chiu H., Irving B., Eaton DL., Grogan JL. Cancer - Interactions investigated in the context of cancer grogan.jane@gene.com Cancer cell 2014 Only protein-protein interactions imex curation imex curation johnston-2014-3 human-t-cell Homo sapiens t-cell Peripheral blood T-lymphocytes fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret antibody detection identification by antibody antibody detection Johnston RJ., Comps-Agrar L., Hackney J., Yu X., Huseni M., Yang Y., Park S., Javinal V., Chiu H., Irving B., Eaton DL., Grogan JL. Cancer - Interactions investigated in the context of cancer After stimulation with anti-CD3 and Cd28 antibodies, T cells were sorted on the basis of TIGIT expression. Endogenous TIGIT and CD226 were labelled with donor flurophore-conjugated anti-TIGIT and acceptor fluorophore-conjugated anti-CD226 antibodies. grogan.jane@gene.com Cancer cell 2014 Only protein-protein interactions imex curation imex curation cd226_human CD226 antigen CD226 DNAM1 DNAX accessory molecule 1 protein protein human Homo sapiens Human MDYPTLLLALLHVYRALCEEVLWHTSVPFAENMSLECVYPSMGILTQVEWFKIGTQQDSIAIFSPTHGMVIRKPYAERVYFLNSTMASNNMTLFFRNASEDDVGYYSCSLYTYPQGTWQKVIQVVQSDSFEAAVPSNSHIVSEPGKNVTLTCQPQMTWPVQAVRWEKIQPRQIDLLTYCNLVHGRNFTSKFPRQIVSNCSHGRWSVIVIPDVTVSDSGLYRCYLQASAGENETFVMRLTVAEGKTDNQYTLFVAGGTVLLLLFVISITTIIVIFLNRRRRRERRDLFTESWDTQKAPNNYRSPISTSQPTNQSMDDTREDIYVNYPTFSRRPKTRV tigit_human T-cell immunoreceptor with Ig and ITIM domains TIGIT VSIG9 VSTM3 V-set and immunoglobulin domain-containing protein 9 V-set and transmembrane domain-containing protein 3 protein protein human Homo sapiens Human MRWCLLLIWAQGLRQAPLASGMMTGTIETTGNISAEKGGSIILQCHLSSTTAQVTQVNWEQQDQLLAICNADLGWHISPSFKDRVAPGPGLGLTLQSLTVNDTGEYFCIYHTYPDGTYTGRIFLEVLESSVAEHGARFQIPLLGAMAATLVVICTAVIVVVALTRKKKALRIHSVEGDLRRKSAGQEEWSPSAPSPPGSCVQAEAAPAGLCGEQRGEDCAELHDYFNVLSYRSLGNCSFFTETG cd226-1 1796654 N/A 1796657 unspecified role unspecified role fluorescence donor fluorescence donor N/A fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 1796657 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept N/A fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association false false false 7 D/E HA-TIGIT (T-cell immunoreceptor with Ig and ITIM domains) cd226-tigit-1 1796654 N/A 1796658 antibody detection identification by antibody antibody detection unspecified role unspecified role fluorescence donor fluorescence donor N/A ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. N/A 1796657 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept N/A flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range false crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association false false false 7 F/H anti-TIGIT antibody tigit-cd226-2 1796655 N/A 1796658 unspecified role unspecified role prey prey N/A ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1796657 unspecified role unspecified role bait bait N/A flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 7G cd226-tigit-2 1796655 N/A 1796658 unspecified role unspecified role bait bait N/A ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1796657 unspecified role unspecified role prey prey N/A flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 7Gi tigit-cd226-1 1796656 N/A 1796657 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept N/A flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 1796658 unspecified role unspecified role fluorescence donor fluorescence donor N/A ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false proximity proximity close-contact colocalization neighbourhood interaction false false false 7 I