IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ gujral-2014-6 A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Gujral T.S., Chan M., Peshkin L., Sorger P.K., Kirschner M.W., MacBeath G. Cancer - Interactions investigated in the context of cancer gavin_macbeath@harvard.edu, marc@hms.harvard.edu Cell (0092-8674) 2014 Only protein-protein interactions imex curation gujral-2014-1 A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. human-focus hcc Human-Focus hepatocellular carcinoma cell line focus_hcc Human FOCUS hepatocellular carcinoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gujral T.S., Chan M., Peshkin L., Sorger P.K., Kirschner M.W., MacBeath G. Cancer - Interactions investigated in the context of cancer gavin_macbeath@harvard.edu, marc@hms.harvard.edu Cell (0092-8674) 2014 Only protein-protein interactions imex curation gujral-2014-2 A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. human-bt_549 Homo sapiens BT_549 Breast ductal carcinoma cell line bt_549 BT 549 Human Breast ductal carcinoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gujral T.S., Chan M., Peshkin L., Sorger P.K., Kirschner M.W., MacBeath G. Cancer - Interactions investigated in the context of cancer gavin_macbeath@harvard.edu, marc@hms.harvard.edu Cell (0092-8674) 2014 Only protein-protein interactions imex curation gujral-2014-3 A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. human-nci_h661 Human lung large cell carcinoma cell line NCIH661 nci_h661 Human lung large cell carcinoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gujral T.S., Chan M., Peshkin L., Sorger P.K., Kirschner M.W., MacBeath G. Cancer - Interactions investigated in the context of cancer gavin_macbeath@harvard.edu, marc@hms.harvard.edu Cell (0092-8674) 2014 Only protein-protein interactions imex curation gujral-2014-4 A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. human-calu_1 human calu_1 calu_1 Human lung carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gujral T.S., Chan M., Peshkin L., Sorger P.K., Kirschner M.W., MacBeath G. Cancer - Interactions investigated in the context of cancer gavin_macbeath@harvard.edu, marc@hms.harvard.edu Cell (0092-8674) 2014 Only protein-protein interactions imex curation gujral-2014-5 A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. in vitro In vitro peptide array peptide array antibody detection identification by antibody antibody detection Gujral T.S., Chan M., Peshkin L., Sorger P.K., Kirschner M.W., MacBeath G. Cancer - Interactions investigated in the context of cancer gavin_macbeath@harvard.edu, marc@hms.harvard.edu Cell (0092-8674) 2014 Only protein-protein interactions imex curation stat3_human Signal transducer and activator of transcription 3 Acute-phase response factor STAT3 APRF protein protein human Homo sapiens Human MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM 6C00632211C8012D fzd2_human Frizzled-2 FZD2 FzE2 protein protein human Homo sapiens Human MRPRSALPRLLLPLLLLPAAGPAQFHGEKGISIPDHGFCQPISIPLCTDIAYNQTIMPNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLEQAIPPCRSICERARQGCEALMNKFGFQWPERLRCEHFPRHGAEQICVGQNHSEDGAPALLTTAPPPGLQPGAGGTPGGPGGGGAPPRYATLEHPFHCPRVLKVPSYLSYKFLGERDCAAPCEPARPDGSMFFSQEETRFARLWILTWSVLCCASTFFTVTTYLVDMQRFRYPERPIIFLSGCYTMVSVAYIAGFVLQERVVCNERFSEDGYRTVVQGTKKEGCTILFMMLYFFSMASSIWWVILSLTWFLAAGMKWGHEAIEANSQYFHLAAWAVPAVKTITILAMGQIDGDLLSGVCFVGLNSLDPLRGFVLAPLFVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEKLERLMVRIGVFSVLYTVPATIVIACYFYEQAFREHWERSWVSQHCKSLAIPCPAHYTPRMSPDFTVYMIKYLMTLIVGITSGFWIWSGKTLHSWRKFYTRLTNSRHGETTV fyn_mouse Tyrosine-protein kinase Fyn p59-Fyn Fyn Proto-oncogene c-Fyn protein protein mouse Mus musculus Mouse MGCVQCKDKEAAKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSERAAGLCCRLVVPCHKGMPRLTDLSVKTKDVWEIPRESLQLIKRLGNGQFGEVWMGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQGFLEDYFTATEPQYQPGENL fyn-fzd2-3 1776201 n/a 1776207 unspecified role unspecified role prey prey human-bt_549 Homo sapiens BT_549 Breast ductal carcinoma cell line bt_549 BT 549 Human Breast ductal carcinoma cell line n/a 1776208 unspecified role unspecified role prey prey human-bt_549 Homo sapiens BT_549 Breast ductal carcinoma cell line bt_549 BT 549 Human Breast ductal carcinoma cell line n/a 1776209 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false sh2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) association association false false false Fig. 5D fyn-fzd2-4 1776201 n/a 1776207 unspecified role unspecified role prey prey human-calu_1 human calu_1 calu_1 Human lung carcinoma cells n/a 1776209 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false sh2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1776208 unspecified role unspecified role prey prey human-calu_1 human calu_1 calu_1 Human lung carcinoma cells association association false false false Fig. 5D fyn-fzd2-5 1776201 n/a 1776207 unspecified role unspecified role prey prey human-focus hcc Human-Focus hepatocellular carcinoma cell line focus_hcc Human FOCUS hepatocellular carcinoma cell line n/a 1776208 unspecified role unspecified role prey prey human-focus hcc Human-Focus hepatocellular carcinoma cell line focus_hcc Human FOCUS hepatocellular carcinoma cell line n/a 1776209 unspecified role unspecified role bait bait sh2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) association association false false false Fig. 5D fzd2-stat3 1776202 n/a 1776207 unspecified role unspecified role prey prey n/a 1776208 unspecified role unspecified role bait bait physical association physical association false false false Fig. 2F stat3-fzd2 1776202 n/a 1776208 unspecified role unspecified role prey prey n/a 1776207 unspecified role unspecified role bait bait physical association physical association false false false Fig. 2F fzd2-stat3-1 1776203 n/a 1776208 unspecified role unspecified role bait bait n/a 1776207 unspecified role unspecified role prey prey physical association physical association false false false Supp. Fig. 3G fzd2-stat3-2 1776204 n/a 1776208 unspecified role unspecified role bait bait n/a 1776207 unspecified role unspecified role prey prey physical association physical association false false false Supp. Fig. 3G fzd2-stat3-3 1776205 n/a 1776208 unspecified role unspecified role bait bait n/a 1776207 unspecified role unspecified role prey prey physical association physical association false false false Supp. Fig. 3G fzd2-fyn 1776206 n/a 1776209 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false sh2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1776208 unspecified role unspecified role bait bait tyr-552 (required) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 1776248 1776246 direct interaction direct interaction false false false Fig. 5C Q14332 increases activity of P39688