IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ gustafson-2014-1 Drugging MYCN through an allosteric transition in Aurora kinase A. human-imr_32 Homo sapiens:Neuroblastoma cell line imr_32 Human IMR-32 Neuroblastoma Human neuroblastoma cell line established from an abdominal mass (diagnosed as neuroblastoma) of a 1-year-old Caucasian boy in 1967. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gustafson W.C., Meyerowitz J.G., Nekritz E.A., Chen J., Benes C., Charron E., Simonds E.F., Seeger R., Matthay K.K., Hertz N.T., Eilers M., Shokat K.M., Weiss W.A. Cancer - Interactions investigated in the context of cancer waweiss@gmail.com waweiss@gmail.com,gustafsonc@peds.ucsf.edu Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation gustafson-2014-2 Drugging MYCN through an allosteric transition in Aurora kinase A. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Gustafson W.C., Meyerowitz J.G., Nekritz E.A., Chen J., Benes C., Charron E., Simonds E.F., Seeger R., Matthay K.K., Hertz N.T., Eilers M., Shokat K.M., Weiss W.A. Cancer - Interactions investigated in the context of cancer waweiss@gmail.com waweiss@gmail.com,gustafsonc@peds.ucsf.edu Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation gustafson-2014-3 Drugging MYCN through an allosteric transition in Aurora kinase A. in vitro In vitro protein kinase assay protein kinase assay autoradiography autoradiography Gustafson W.C., Meyerowitz J.G., Nekritz E.A., Chen J., Benes C., Charron E., Simonds E.F., Seeger R., Matthay K.K., Hertz N.T., Eilers M., Shokat K.M., Weiss W.A. Cancer - Interactions investigated in the context of cancer In Supplementary figure 1B and C legend it is stated that:- "Enzyme was either full-length or kinase domain-only Aurora A, and substrate was either full-length purified Histone H3 or target oligopeptide." The target oligopeptide has been entered here as the exact identity of the H3 molecule used is unknown and the origin of full length Aurora A is not stated. waweiss@gmail.com waweiss@gmail.com,gustafsonc@peds.ucsf.edu Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation gustafson-2014-4 Drugging MYCN through an allosteric transition in Aurora kinase A. human-sk_n_be(2) Human caucasian neuroblastoma cell line sk_n_be(2) Human neuroblastoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gustafson W.C., Meyerowitz J.G., Nekritz E.A., Chen J., Benes C., Charron E., Simonds E.F., Seeger R., Matthay K.K., Hertz N.T., Eilers M., Shokat K.M., Weiss W.A. Cancer - Interactions investigated in the context of cancer waweiss@gmail.com waweiss@gmail.com,gustafsonc@peds.ucsf.edu Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation mycn_human N-myc proto-oncogene protein MYCN NMYC BHLHE37 Class E basic helix-loop-helix protein 37 protein protein human Homo sapiens Human MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC aurka_human Aurora kinase A AIRK1 Serine/threonine-protein kinase 15 Aurora/IPL1-related kinase 1 Breast tumor-amplified kinase STK6 AIK ARK1 AURA BTAK STK15 AURKA AYK1 IAK1 Aurora 2 Serine/threonine-protein kinase 6 Serine/threonine-protein kinase aurora-A protein protein human Homo sapiens Human MDRSKENCISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVPLQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQETYKRISRVEFTFPDFVTEGARDLISRLLKHNPSQRPMLREVLEHPWITANSSKPSNCQNKESASKQS cd532 CD532 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) rs6_human 40S ribosomal protein S6 RPS6 Phosphoprotein NP33 Small ribosomal subunit protein eS6 OK/SW-cl.2 protein protein human Homo sapiens Human MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK max_human Protein max MAX BHLHD4 Class D basic helix-loop-helix protein 4 Myc-associated factor X protein protein human Homo sapiens Human MSDNDDIEVESDEEQPRFQSAADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS mycn-aurka-2 1574888 n/a 1574892 unspecified role unspecified role bait bait n/a 1574893 unspecified role unspecified role prey prey physical association physical association false false false 8 A/B CD532 - an ATP-mimetic molecule, and Conformation-Disrupting Aurora A inhibitor, which causes a dose-dependent and complete dissociation of the MYCN-Aurora-A complex. CD532 MLN8237 -a small molecule specific Aurora A inhibitor, and a less effective disruptor of the this interaction than CD532. aurka-cd532 1574889 n/a 1574894 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1574893 unspecified role unspecified role neutral component neutral component kinase_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O14965:p.Thr287Asp mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Supplementary Table 1 (fig 3) Working: 17.82%, Free: 21.8% 1.853 A aurka-cd532-2 1574890 n/a 1574895 enzyme target enzyme target substrate substrate neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1574894 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) n/a 1574893 enzyme enzyme neutral component neutral component kinase_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O14965:p.Thr287Asp mutation mutation certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false Supplementary Fig 1B/C P62753 opser CHEBI:83432 decreases activity of O14965 mycn-aurka-4 1574891 n/a 1574896 unspecified role unspecified role prey prey n/a 1574893 unspecified role unspecified role prey prey n/a 1574892 unspecified role unspecified role bait bait physical association physical association false false false Supp 6A/B CD532 causes a dissociation of the MYCN-Aurora-A complex; the effect of CD532 on the MYCN-Aurora-A interaction was specific in that it did not affect MYCN-MAX binding.