IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ khandanpour-2013-5 Growth Factor Independence 1 Antagonizes a p53-Induced DNA Damage Response Pathway in Lymphoblastic Leukemia. mouse-thymocyte MOUSE THYMOCYTE thymocyte THYMOCYTE ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Khandanpour C., Phelan J.D., Vassen L., Schütte J., Chen R., Horman S.R., Gaudreau M.C., Krongold J., Zhu J., Paul W.E., Dührsen U., Göttgens B., Grimes H.L., Möröy T. Cancer - Interactions investigated in the context of cancer Thymocytes were irradiated (5 Gy) and chromatin was generated either at 90 minutes later (Fig S4 K/L) or at 0, 30, 60, 90 and 120 minutes later (Fig S4 M). The authors state that the Gfi1-Flag contruct used in Fig 5H contains the human GFI1 sequence. lee.grimes@cchmc.org,tarik.moroy@ircm.qc.ca Tarik.Moroy@ircm.qc.ca,Lee.Grimes@cchmc.org Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation khandanpour-2013-3 Growth Factor Independence 1 Antagonizes a p53-Induced DNA Damage Response Pathway in Lymphoblastic Leukemia. mouse-hematopoietic progenitors MOUSE bone marrow hematopoietic progenitor cells hematopoietic progenitor cells Bone marrow hematopoietic progenitor cells/HSC-haematopoietic stem cells ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Khandanpour C., Phelan J.D., Vassen L., Schütte J., Chen R., Horman S.R., Gaudreau M.C., Krongold J., Zhu J., Paul W.E., Dührsen U., Göttgens B., Grimes H.L., Möröy T. Cancer - Interactions investigated in the context of cancer Cells immortalized by retroviral transduction of an MLL-ENL expression vector. Sequencing was carried out using an Illumina Genome Analyzer. The authors state that the Gfi1-Flag contruct used in Fig 5H contains the human GFI1 sequence. lee.grimes@cchmc.org,tarik.moroy@ircm.qc.ca Tarik.Moroy@ircm.qc.ca,Lee.Grimes@cchmc.org Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation khandanpour-2013-4 Growth Factor Independence 1 Antagonizes a p53-Induced DNA Damage Response Pathway in Lymphoblastic Leukemia. mouse-thymocyte MOUSE THYMOCYTE thymocyte THYMOCYTE ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Khandanpour C., Phelan J.D., Vassen L., Schütte J., Chen R., Horman S.R., Gaudreau M.C., Krongold J., Zhu J., Paul W.E., Dührsen U., Göttgens B., Grimes H.L., Möröy T. Cancer - Interactions investigated in the context of cancer Primers were from Gfi1-bound regions. Assays were carried out before and after irradiation. The authors state that the Gfi1-Flag contruct used in Fig 5H contains the human GFI1 sequence. lee.grimes@cchmc.org,tarik.moroy@ircm.qc.ca Tarik.Moroy@ircm.qc.ca,Lee.Grimes@cchmc.org Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation khandanpour-2013-1 Growth Factor Independence 1 Antagonizes a p53-Induced DNA Damage Response Pathway in Lymphoblastic Leukemia. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Khandanpour C., Phelan J.D., Vassen L., Schütte J., Chen R., Horman S.R., Gaudreau M.C., Krongold J., Zhu J., Paul W.E., Dührsen U., Göttgens B., Grimes H.L., Möröy T. Cancer - Interactions investigated in the context of cancer The authors state that the Gfi1-Flag contruct used in Fig 5H contains the human GFI1 sequence. lee.grimes@cchmc.org,tarik.moroy@ircm.qc.ca Tarik.Moroy@ircm.qc.ca,Lee.Grimes@cchmc.org Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation khandanpour-2013-2 Growth Factor Independence 1 Antagonizes a p53-Induced DNA Damage Response Pathway in Lymphoblastic Leukemia. mouse-thymocyte MOUSE THYMOCYTE thymocyte THYMOCYTE anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Khandanpour C., Phelan J.D., Vassen L., Schütte J., Chen R., Horman S.R., Gaudreau M.C., Krongold J., Zhu J., Paul W.E., Dührsen U., Göttgens B., Grimes H.L., Möröy T. Cancer - Interactions investigated in the context of cancer 10Gy gamma- irradiation The authors state that the Gfi1-Flag contruct used in Fig 5H contains the human GFI1 sequence. lee.grimes@cchmc.org,tarik.moroy@ircm.qc.ca Tarik.Moroy@ircm.qc.ca,Lee.Grimes@cchmc.org Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD bax_mouse_gene BAX MOUSE GENE gene gene mouse Mus musculus Mouse apr_mouse_gene Pmaip1 /Noxa gene Mouse Gene gene gene mouse Mus musculus Mouse bbc3_mouse_gene BBC3_MOUSE_GENE gene gene mouse Mus musculus Mouse gfi1_mouse Zinc finger protein Gfi-1 Gfi1 Gfi-1 Growth factor independent protein 1 protein protein mouse Mus musculus Mouse MPRSFLVKSKKAHSYHQPRSPGPDYSLRLETVPAPGRAEGGAVSAGESKMEPRERLSPDSQLTEAPDRASASPNSCEGSVCDPCSEFEDFWRPPSPSVSPASEKSLCRSLDEAQPYTLPFKPYAWSGLAGSDLRHLVQSYRQCSALERSAGLSLFCERGSEPGRPAARYGPEQAAGGAGAGQPGRCGVAGGATSAAGLGLYGDFAPAAAGLYERPSTAAGRLYQDHGHELHADKSVGVKVESELLCTRLLLGGGSYKCIKCSKVFSTPHGLEVHVRRSHSGTRPFACEMCGKTFGHAVSLEQHKAVHSQERSFDCKICGKSFKRSSTLSTHLLIHSDTRPYPCQYCGKRFHQKSDMKKHTFIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFGCDLCGKGFQRKVDLRRHRETQHGLK cdn1a_mouse_gene CDN1A_MOUSE_GENE gene gene mouse Mus musculus Mouse cep95_mouse_gene CEP95_MOUSE_GENE/Ccdc45 gene gene mouse Mus musculus Mouse pten_mouse_gene PTEN_MOUSE_GENE gene gene mouse Mus musculus Mouse ddb2_mouse_gene DDB2_MOUSE GENE gene gene mouse Mus musculus Mouse id2_mouse_gene Mouse Id2 gene gene gene mouse Mus musculus Mouse egr2_mouse_gene EGR2 MOUSE GENE gene gene mouse Mus musculus Mouse jun_mouse_gene JUN MOUSE GENE gene gene mouse Mus musculus Mouse vdr_mouse_gene VDR MOUSE GENE gene gene mouse Mus musculus Mouse p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD gfi1_human Zinc finger protein Gfi-1 Growth factor independent protein 1 Zinc finger protein 163 GFI1 ZNF163 protein protein human Homo sapiens Human MPRSFLVKSKKAHSYHQPRSPGPDYSLRLENVPAPSRADSTSNAGGAKAEPRDRLSPESQLTEAPDRASASPDSCEGSVCERSSEFEDFWRPPSPSASPASEKSMCPSLDEAQPFPLPFKPYSWSGLAGSDLRHLVQSYRPCGALERGAGLGLFCEPAPEPGHPAALYGPKRAAGGAGAGAPGSCSAGAGATAGPGLGLYGDFGSAAAGLYERPTAAAGLLYPERGHGLHADKGAGVKVESELLCTRLLLGGGSYKCIKCSKVFSTPHGLEVHVRRSHSGTRPFACEMCGKTFGHAVSLEQHKAVHSQERSFDCKICGKSFKRSSTLSTHLLIHSDTRPYPCQYCGKRFHQKSDMKKHTFIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFGCDLCGKGFQRKVDLRRHRETQHGLK gfi1-mouse-6 1371082 n/a 1371087 unspecified role unspecified role bait bait n/a 1371088 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false S4 L/M Co-occupation by Gfi1 and p53 is maintained over time, but p53 occupation clearly dominates at 120 min after the initial DNA damage signal over Gfi1. gfi1-mouse-6 1371082 n/a 1371087 unspecified role unspecified role bait bait n/a 1371089 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false S4 L/M Co-occupation by Gfi1 and p53 is maintained over time, but p53 occupation clearly dominates at 120 min after the initial DNA damage signal over Gfi1. gfi1-bbc3-2 1371082 n/a 1371090 unspecified role unspecified role prey prey n/a 1371087 unspecified role unspecified role bait bait association association false false false S4 L/M Co-occupation by Gfi1 and p53 is maintained over time, but p53 occupation clearly dominates at 120 min after the initial DNA damage signal over Gfi1. gfi1-mouse-7 1371082 n/a 1371091 unspecified role unspecified role bait bait n/a 1371088 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false S4 K/M Co-occupation by Gfi1 and p53 is maintained over time, but p53 occupation clearly dominates at 120 min after the initial DNA damage signal over Gfi1. gfi1-mouse-8 1371082 n/a 1371091 unspecified role unspecified role bait bait n/a 1371089 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false S4 K/M Co-occupation by Gfi1 and p53 is maintained over time, but p53 occupation clearly dominates at 120 min after the initial DNA damage signal over Gfi1. gfi1-bbc3-3 1371082 n/a 1371091 unspecified role unspecified role bait bait n/a 1371090 unspecified role unspecified role prey prey association association false false false S4 K/M Co-occupation by Gfi1 and p53 is maintained over time, but p53 occupation clearly dominates at 120 min after the initial DNA damage signal over Gfi1. gfi1-bbc3-4 1371082 n/a 1371091 unspecified role unspecified role bait bait n/a 1371092 unspecified role unspecified role prey prey association association false false false S4 K/M Co-occupation by Gfi1 and p53 is maintained over time, but p53 occupation clearly dominates at 120 min after the initial DNA damage signal over Gfi1. tp53-bbc3-6 1371082 n/a 1371092 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1371087 unspecified role unspecified role bait bait association association false false false S4 L/M Co-occupation by Gfi1 and p53 is maintained over time, but p53 occupation clearly dominates at 120 min after the initial DNA damage signal over Gfi1. gfi1-mouse-9 1371082 n/a 1371091 unspecified role unspecified role bait bait n/a 1371093 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false S4 K gfi1-bbc3-5 1371082 n/a 1371091 unspecified role unspecified role bait bait n/a 1371094 unspecified role unspecified role prey prey association association false false false S4 K gfi1-bbc3-6 1371082 n/a 1371091 unspecified role unspecified role bait bait n/a 1371095 unspecified role unspecified role prey prey association association false false false S4 K tp53-bbc3-8 1371082 n/a 1371095 unspecified role unspecified role prey prey n/a 1371087 unspecified role unspecified role bait bait association association false false false S4 L gfi1-bbc3-7 1371082 n/a 1371091 unspecified role unspecified role bait bait n/a 1371096 unspecified role unspecified role prey prey association association false false false S4 K tp53-bbc3-9 1371082 n/a 1371096 unspecified role unspecified role prey prey n/a 1371087 unspecified role unspecified role bait bait association association false false false S4 L gfi1-bbc3-8 1371082 n/a 1371091 unspecified role unspecified role bait bait n/a 1371097 unspecified role unspecified role prey prey association association false false false S4 K tp53-ddb2 1371082 n/a 1371097 unspecified role unspecified role prey prey n/a 1371087 unspecified role unspecified role bait bait association association false false false S4 L gfi1-bbc3-9 1371082 n/a 1371091 unspecified role unspecified role bait bait n/a 1371098 unspecified role unspecified role prey prey association association false false false S4 K gfi1-mouse-11 1371082 n/a 1371091 unspecified role unspecified role bait bait n/a 1371099 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false S4 K gfi1-mouse 1371083 n/a 1371091 unspecified role unspecified role bait bait n/a 1371088 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5Dii gfi1-mouse-1 1371083 n/a 1371091 unspecified role unspecified role bait bait n/a 1371089 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5D gfi1-mouse-2 1371083 n/a 1371091 unspecified role unspecified role bait bait n/a 1371090 unspecified role unspecified role prey prey association association false false false 5Diii gfi1-mouse-5 1371084 n/a 1371088 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1371087 unspecified role unspecified role bait bait association association false false false 5Eii 6Gy gamma irradiation gfi1-mouse-5 1371084 n/a 1371087 unspecified role unspecified role bait bait n/a 1371089 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5E 6Gy gamma irradiation gfi1-bbc3-1 1371084 n/a 1371090 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1371087 unspecified role unspecified role bait bait association association false false false 5Eiii 6Gy gamma irradiation gfi1-tp53-1 1371085 n/a 1371100 unspecified role unspecified role prey prey possible_phospho-ser15 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false n/a 1371101 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 5H gamma-ray irradiation (6Gy) increased levels of S15 phosphorylated- p53 detected. gfi1-tp53-2 1371086 n/a 1371091 unspecified role unspecified role bait bait n/a 1371087 unspecified role unspecified role prey prey possible_phosph-ser15 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5I