IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ lu-2012-6 CREPT Accelerates Tumorigenesis by Regulating the Transcription of Cell-Cycle-Related Genes. human-mgc-803 Homo sapiens mgc-803 Human-poorly differentiated human gastric adenocarcinoma MGC803 Original paper: - no pmid available. K.H. Wang The establishment and biological characteristics of a poorly differentiated human gastric adenocarcinoma MGC803 cell line. Acta Biol. Exp. Sin., 16 (1983), pp. 257–267 ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Lu D., Wu Y., Wang Y., Ren F., Wang D., Su F., Zhang Y., Yang X., Jin G., Hao X., He D., Zhai Y., Irwin D.M., Hu J., Sung J.J., Yu J., Jia B., Chang Z. Cancer - Interactions investigated in the context of cancer The authors confirm 293T was the cell line used. zhijiec@tsinghua.edu.cn, baoqingjia@126.com Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation lu-2012-8 CREPT Accelerates Tumorigenesis by Regulating the Transcription of Cell-Cycle-Related Genes. human-colon_cancer human colonic cancer tissue Human colon_cancer Colon cancer tisuue ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Lu D., Wu Y., Wang Y., Ren F., Wang D., Su F., Zhang Y., Yang X., Jin G., Hao X., He D., Zhai Y., Irwin D.M., Hu J., Sung J.J., Yu J., Jia B., Chang Z. Cancer - Interactions investigated in the context of cancer The authors confirm 293T was the cell line used. zhijiec@tsinghua.edu.cn, baoqingjia@126.com Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation lu-2012-1 CREPT Accelerates Tumorigenesis by Regulating the Transcription of Cell-Cycle-Related Genes. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Lu D., Wu Y., Wang Y., Ren F., Wang D., Su F., Zhang Y., Yang X., Jin G., Hao X., He D., Zhai Y., Irwin D.M., Hu J., Sung J.J., Yu J., Jia B., Chang Z. Cancer - Interactions investigated in the context of cancer The authors confirm 293T was the cell line used. zhijiec@tsinghua.edu.cn, baoqingjia@126.com Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation lu-2012-2 CREPT Accelerates Tumorigenesis by Regulating the Transcription of Cell-Cycle-Related Genes. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lu D., Wu Y., Wang Y., Ren F., Wang D., Su F., Zhang Y., Yang X., Jin G., Hao X., He D., Zhai Y., Irwin D.M., Hu J., Sung J.J., Yu J., Jia B., Chang Z. Cancer - Interactions investigated in the context of cancer The authors confirm 293T was the cell line used. zhijiec@tsinghua.edu.cn, baoqingjia@126.com Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation lu-2012-3 CREPT Accelerates Tumorigenesis by Regulating the Transcription of Cell-Cycle-Related Genes. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu D., Wu Y., Wang Y., Ren F., Wang D., Su F., Zhang Y., Yang X., Jin G., Hao X., He D., Zhai Y., Irwin D.M., Hu J., Sung J.J., Yu J., Jia B., Chang Z. Cancer - Interactions investigated in the context of cancer The authors confirm 293T was the cell line used. zhijiec@tsinghua.edu.cn, baoqingjia@126.com Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation lu-2012-4 CREPT Accelerates Tumorigenesis by Regulating the Transcription of Cell-Cycle-Related Genes. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Lu D., Wu Y., Wang Y., Ren F., Wang D., Su F., Zhang Y., Yang X., Jin G., Hao X., He D., Zhai Y., Irwin D.M., Hu J., Sung J.J., Yu J., Jia B., Chang Z. Cancer - Interactions investigated in the context of cancer The authors confirm 293T was the cell line used. After formaldehyde fixation an IP was carried out. zhijiec@tsinghua.edu.cn, baoqingjia@126.com Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation lu-2012-5 CREPT Accelerates Tumorigenesis by Regulating the Transcription of Cell-Cycle-Related Genes. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Lu D., Wu Y., Wang Y., Ren F., Wang D., Su F., Zhang Y., Yang X., Jin G., Hao X., He D., Zhai Y., Irwin D.M., Hu J., Sung J.J., Yu J., Jia B., Chang Z. Cancer - Interactions investigated in the context of cancer The authors confirm 293T was the cell line used. zhijiec@tsinghua.edu.cn, baoqingjia@126.com Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation lu-2012-7 CREPT Accelerates Tumorigenesis by Regulating the Transcription of Cell-Cycle-Related Genes. human-mgc-803 Homo sapiens mgc-803 Human-poorly differentiated human gastric adenocarcinoma MGC803 Original paper: - no pmid available. K.H. Wang The establishment and biological characteristics of a poorly differentiated human gastric adenocarcinoma MGC803 cell line. Acta Biol. Exp. Sin., 16 (1983), pp. 257–267 ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Lu D., Wu Y., Wang Y., Ren F., Wang D., Su F., Zhang Y., Yang X., Jin G., Hao X., He D., Zhai Y., Irwin D.M., Hu J., Sung J.J., Yu J., Jia B., Chang Z. Cancer - Interactions investigated in the context of cancer The authors confirm 293T was the cell line used. ChIP-3C (Chromosome Conformation Capture Assay) zhijiec@tsinghua.edu.cn, baoqingjia@126.com Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation lu-2012-9 CREPT Accelerates Tumorigenesis by Regulating the Transcription of Cell-Cycle-Related Genes. human-colon_cancer human colonic cancer tissue Human colon_cancer Colon cancer tisuue ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Lu D., Wu Y., Wang Y., Ren F., Wang D., Su F., Zhang Y., Yang X., Jin G., Hao X., He D., Zhai Y., Irwin D.M., Hu J., Sung J.J., Yu J., Jia B., Chang Z. Cancer - Interactions investigated in the context of cancer The authors confirm 293T was the cell line used. ChIP-3C (Chromosome Conformation Capture Assay) zhijiec@tsinghua.edu.cn, baoqingjia@126.com Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation ccnd1_human_gene Human Cyclin D1 gene gene gene human Homo sapiens Human rpr1b_human Regulation of nuclear pre-mRNA domain-containing protein 1B RPRD1B C20orf77 CREPT Cell cycle-related and expression-elevated protein in tumor protein protein human Homo sapiens Human MSSFSESALEKKLSELSNSQQSVQTLSLWLIHHRKHAGPIVSVWHRELRKAKSNRKLTFLYLANDVIQNSKRKGPEFTREFESVLVDAFSHVAREADEGCKKPLERLLNIWQERSVYGGEFIQQLKLSMEDSKSPPPKATEEKKSLKRTFQQIQEEEDDDYPGSYSPQDPSAGPLLTEELIKALQDLENAASGDATVRQKIASLPQEVQDVSLLEKITDKEAAERLSKTVDEACLLLAEYNGRLAAELEDRRQLARMLVEYTQNQKDVLSEKEKKLEEYKQKLARVTQVRKELKSHIQSLPDLSLLPNVTGGLAPLPSAGDLFSTD rpb1_human DNA-directed RNA polymerase II subunit RPB1 POLR2A DNA-directed RNA polymerase II subunit A DNA-directed RNA polymerase III largest subunit POLR2 RNA-directed RNA polymerase II subunit RPB1 protein protein human Homo sapiens Human MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMEIPTNIPGLGAAGPTGMFFGSAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMTPGAAGFSPSAASDASGFSPGYSPAWSPTPGSPGSPGPSSPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPNYTPTSPSYSPTSPSYSPTSPNYTPTSPNYSPTSPSYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPASPKYTPTSPSYSPSSPEYTPTSPKYSPTSPKYSPTSPKYSPTSPTYSPTTPKYSPTSPTYSPTSPVYTPTSPKYSPTSPTYSPTSPKYSPTSPTYSPTSPKGSTYSPTSPGYSPTSPTYSLTSPAISPDDSDEEN rprd1b-ccnd1 1266486 n/a 1266495 unspecified role unspecified role prey prey pre-poly(a) cleavage site binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false promoter_p2 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1266496 unspecified role unspecified role bait bait association association false false false 6B/C CREPT crosslinks strongly to a CYCLIN D1 promoter region (P2) , and also to a region related to transcription termination at the 3′ end of the gene. polr2a-ccnd1 1266486 n/a 1266495 unspecified role unspecified role prey prey promoter binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1266497 unspecified role unspecified role bait bait association association false false false 6D/E CREPT expression reduced RNAPII binding to region G - after the poly(A) site. CREPT rprd1b-ccnd1-3 1266487 n/a 1266496 unspecified role unspecified role bait bait n/a 1266495 unspecified role unspecified role prey prey promoter_p2 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false pre-poly(a) cleavage site binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 7C CREPT crosslinks strongly to a CYCLIN D1 promoter region (P2) , and also to a region related to transcription termination at the 3′ end of the gene. polr2a-ccnd1-3 1266487 n/a 1266497 unspecified role unspecified role bait bait n/a 1266495 unspecified role unspecified role prey prey promoter_p2 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false pre-poly(a) cleavage site binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 7D CREPT expression reduced RNAPII binding to region G - after the poly(A) site. CREPT rprd1b-polr2a 1266488 n/a 1266497 unspecified role unspecified role prey prey n/a 1266496 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 5A rprd1b-polr2a-1 1266489 n/a 1266497 unspecified role unspecified role prey prey n/a 1266496 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rpr_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 5B myc-CREPT rather than flag-CREPT is stated to have been used in the figure legend. rprd1b-polr2a-2 1266490 n/a 1266497 unspecified role unspecified role prey prey n/a 1266496 unspecified role unspecified role bait bait physical association physical association false false false Fig 5C polr2a-rprd1b 1266491 n/a 1266496 unspecified role unspecified role prey prey n/a 1266497 unspecified role unspecified role bait bait physical association physical association false false false Fig 5D rprd1b-polr2a-3 1266492 n/a 1266496 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1266497 unspecified role unspecified role prey prey human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false Fig 5E rprd1b-ccnd1-2 1266493 n/a 1266495 unspecified role unspecified role prey prey b1_promoter_and_f1_pre-poly(a)- cleavage site_regions binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1266496 unspecified role unspecified role bait bait association association false false false 6I The anti-RNAPII antibody precipitated down a strong cycled band (chromatin loop) when CREPT was overexpressed. CREPT rprd1b-ccnd1-4 1266494 n/a 1266495 unspecified role unspecified role prey prey b1_promoter_and_f1_pre-poly(a)- cleavage site_regions binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1266496 unspecified role unspecified role bait bait association association false false false 6I The anti-RNAPII antibody precipitated down a strong cycled band (chromatin loop) when CREPT was overexpressed. CREPT