I2D I2D leung-2011-1 A novel interplay between oncogenic PFTK1 protein kinase and tumor suppressor TAGLN2 in the control of liver cancer cell motility. human-hep_3b Homo sapiens hepatocellular carcinoma (HCC) cells (p53-null). hep_3b human hepatocellular carcinoma (HCC) cells (p53-null). anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Leung W.K., Ching A.K., Chan A.W., Poon T.C., Mian H., Wong A.S., To K.F., Wong N. Cancer - Interactions investigated in the context of cancer natwong@cuhk.edu.hk Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database leung-2011-2 A novel interplay between oncogenic PFTK1 protein kinase and tumor suppressor TAGLN2 in the control of liver cancer cell motility. human-hkcl-4 Human hepatocellular carcinoma hkcl-4 Human hepatocellular carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Leung W.K., Ching A.K., Chan A.W., Poon T.C., Mian H., Wong A.S., To K.F., Wong N. Cancer - Interactions investigated in the context of cancer natwong@cuhk.edu.hk Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database leung-2011-3 A novel interplay between oncogenic PFTK1 protein kinase and tumor suppressor TAGLN2 in the control of liver cancer cell motility. human-l02 Immortal human liver cell line l02 Immortal human liver cell line Cell Bank No. GNHu6, China anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Leung W.K., Ching A.K., Chan A.W., Poon T.C., Mian H., Wong A.S., To K.F., Wong N. Cancer - Interactions investigated in the context of cancer natwong@cuhk.edu.hk Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database actb_human Actin, cytoplasmic 1 ACTB Beta-actin protein protein human Homo sapiens Human MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF tagl2_human Transgelin-2 TAGLN2 KIAA0120 SM22-alpha homolog CDABP0035 Epididymis tissue protein Li 7e protein protein human Homo sapiens Human MANRGPAYGLSREVQQKIEKQYDADLEQILIQWITTQCRKDVGRPQPGRENFQNWLKDGTVLCELINALYPEGQAPVKKIQASTMAFKQMEQISQFLQAAERYGINTTDIFQTVDLWEGKNMACVQRTLMNLGGLAVARDDGLFSGDPNWFPKKSKENPRNFSDNQLQEGKNVIGLQMGTNRGASQAGMTGYGMPRQIL tagln2-actb-4 1239453 n/a 1239456 unspecified role unspecified role prey prey n/a 1239457 unspecified role unspecified role bait bait P37802:p.Ser163Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false P37802:p.Ser83Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false P37802:p.Ser83Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P37802:p.Ser163Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4ci, 7ai, 7bi Knockdown of PFTK1 with sh-RNA results in increased association of TAGLN2 with ACTB. Phospho-defective mutants S83A and S163A mutants abrogated the inhibitory effect of PFTK1. Phospho-mimetic mutants S83D and S163D mutants decreased Actin binding in PFTK-suppressed Hep3B cells. tagln2-actb-1 1239454 n/a 1239457 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1239456 unspecified role unspecified role prey prey physical association physical association false false false 4cii Knockdown of PFTK1 results in increased association of TAGLN2 with ACTB. tagln2-actb-2 1239455 n/a 1239456 unspecified role unspecified role prey prey n/a 1239457 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 4ciii PFTK1 expression results in decreased association of TAGLN2 with ACTB.