I2D I2D lara-2011-1 An siRNA screen identifies RSK1 as a key modulator of lung cancer metastasis. human-a_549 Homo sapiens Caucasian lung carcinoma cell line a_549 Human Caucasian lung carcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lara R., Mauri F.A., Taylor H., Derua R., Shia A., Gray C., Nicols A., Shiner R.J., Schofield E., Bates P.A., Waelkens E., Dallman M., Lamb J., Zicha D., Downward J., Seckl M.J., Pardo O.E. Cancer - Interactions investigated in the context of cancer m.seckl@imperial.ac.uk Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database lara-2011-2 An siRNA screen identifies RSK1 as a key modulator of lung cancer metastasis. in vitro In vitro protein kinase assay protein kinase assay autoradiography autoradiography Lara R., Mauri F.A., Taylor H., Derua R., Shia A., Gray C., Nicols A., Shiner R.J., Schofield E., Bates P.A., Waelkens E., Dallman M., Lamb J., Zicha D., Downward J., Seckl M.J., Pardo O.E. Cancer - Interactions investigated in the context of cancer Author confirms that all proteins used are human. The recombinant ribosomal S6 protein (P62753) used in the kinase assay is a C-term GST fusion recombinant S6 protein (amino acid 218 to 249). m.seckl@imperial.ac.uk Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database lara-2011-3 An siRNA screen identifies RSK1 as a key modulator of lung cancer metastasis. in vitro In vitro protein kinase assay protein kinase assay de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Lara R., Mauri F.A., Taylor H., Derua R., Shia A., Gray C., Nicols A., Shiner R.J., Schofield E., Bates P.A., Waelkens E., Dallman M., Lamb J., Zicha D., Downward J., Seckl M.J., Pardo O.E. Cancer - Interactions investigated in the context of cancer Author confirms that all proteins used are human. m.seckl@imperial.ac.uk Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database vasp_human Vasodilator-stimulated phosphoprotein VASP protein protein human Homo sapiens Human MSETVICSSRATVMLYDDGNKRWLPAGTGPQAFSRVQIYHNPTANSFRVVGRKMQPDQQVVINCAIVRGVKYNQATPNFHQWRDARQVWGLNFGSKEDAAQFAAGMASALEALEGGGPPPPPALPTWSVPNGPSPEEVEQQKRQQPGPSEHIERRVSNAGGPPAPPAGGPPPPPGPPPPPGPPPPPGLPPSGVPAAAHGAGGGPPPAPPLPAAQGPGGGGAGAPGLAAAIAGAKLRKVSKQEEASGGPTAPKAESGRSGGGGLMEEMNAMLARRRKATQVGEKTPKDESANQEEPEARVPAQSESVRRPWEKNSTTLPRMKSSSSVTTSETQPCTPSSSDYSDLQRVKQELLEEVKKELQKVKEEIIEAFVQELRKRGSP ks6a1_human Ribosomal protein S6 kinase alpha-1 RPS6KA1 RSK1 90 kDa ribosomal protein S6 kinase 1 MAPKAPK1A Ribosomal S6 kinase 1 MAP kinase-activated protein kinase 1a protein protein human Homo sapiens Human MPLAQLKEPWPLMELVPLDPENGQTSGEEAGLQPSKDEGVLKEISITHHVKAGSEKADPSHFELLKVLGQGSFGKVFLVRKVTRPDSGHLYAMKVLKKATLKVRDRVRTKMERDILADVNHPFVVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEVMFTEEDVKFYLAELALGLDHLHSLGIIYRDLKPENILLDEEGHIKLTDFGLSKEAIDHEKKAYSFCGTVEYMAPEVVNRQGHSHSADWWSYGVLMFEMLTGSLPFQGKDRKETMTLILKAKLGMPQFLSTEAQSLLRALFKRNPANRLGSGPDGAEEIKRHVFYSTIDWNKLYRREIKPPFKPAVAQPDDTFYFDTEFTSRTPKDSPGIPPSAGAHQLFRGFSFVATGLMEDDGKPRAPQAPLHSVVQQLHGKNLVFSDGYVVKETIGVGSYSECKRCVHKATNMEYAVKVIDKSKRDPSEEIEILLRYGQHPNIITLKDVYDDGKHVYLVTELMRGGELLDKILRQKFFSEREASFVLHTIGKTVEYLHSQGVVHRDLKPSNILYVDESGNPECLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQGYDEGCDIWSLGILLYTMLAGYTPFANGPSDTPEEILTRIGSGKFTLSGGNWNTVSETAKDLVSKMLHVDPHQRLTAKQVLQHPWVTQKDKLPQSQLSHQDLQLVKGAMAATYSALNSSKPTPQLKPIESSILAQRRVRKLPSTTL enah_human Protein enabled homolog ENAH MENA protein protein human Homo sapiens Human MSEQSICQARAAVMVYDDANKKWVPAGGSTGFSRVHIYHHTGNNTFRVVGRKIQDHQVVINCAIPKGLKYNQATQTFHQWRDARQVYGLNFGSKEDANVFASAMMHALEVLNSQETGPTLPRQNSQLPAQVQNGPSQEELEIQRRQLQEQQRQKELERERLERERMERERLERERLERERLERERLEQEQLERERQERERQERLERQERLERQERLERQERLDRERQERQERERLERLERERQERERQEQLEREQLEWERERRISSAAAPASVETPLNSVLGDSSASEPGLQAASQPAETPSQQGIVLGPLAPPPPPPLPPGPAQASVALPPPPGPPPPPPLPSTGPPPPPPPPPLPNQVPPPPPPPPAPPLPASGFFLASMSEDNRPLTGLAAAIAGAKLRKVSRMEDTSFPSGGNAIGVNSASSKTDTGRGNGPLPLGGSGLMEEMSALLARRRRIAEKGSTIETEQKEDKGEDSEPVTSKASSTSTPEPTRKPWERTNTMNGSKSPVISRRDSPRKNQIVFDNRSYDSLHRPKSTPLSQPSANGVQTEGLDYDRLKQDILDEMRKELTKLKEELIDAIRQELSKSNTA rs6_human 40S ribosomal protein S6 RPS6 Phosphoprotein NP33 Small ribosomal subunit protein eS6 OK/SW-cl.2 protein protein human Homo sapiens Human MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKGHSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRKPLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRASTSKSESSQK rps6ka1-vasp 1175117 n/a 1175120 unspecified role unspecified role prey prey n/a 1175121 unspecified role unspecified role bait bait physical association physical association false false false 3c vasp-rps6ka1 1175117 n/a 1175121 unspecified role unspecified role prey prey n/a 1175120 unspecified role unspecified role bait bait physical association physical association false false false 3c rps6ka1-enah 1175117 n/a 1175121 unspecified role unspecified role bait bait n/a 1175122 unspecified role unspecified role prey prey physical association physical association false false false 3d rps6ka1-vasp-1 1175118 n/a 1175120 enzyme target enzyme target substrate substrate unspecified role unspecified role c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. n/a 1175121 enzyme enzyme unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false phosphorylation phosphorylation reaction phosphorylation false false false 3e rps6-rps6ka1 1175118 n/a 1175123 enzyme target enzyme target substrate substrate unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1175121 enzyme enzyme unspecified role unspecified role phosphorylation phosphorylation reaction phosphorylation false false false 3e Author confirms protein identity. rps6ka1-vasp-2 1175119 n/a 1175120 enzyme target enzyme target substrate substrate unspecified role unspecified role n/a 1175121 enzyme enzyme unspecified role unspecified role phosphorylation phosphorylation reaction phosphorylation false false false S5 VASP is phosphorylated on Thr278.