IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ proietti-2011-2 Novel role of signal transducer and activator of transcription 3 as a progesterone receptor coactivator in breast cancer. human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Proietti C.J., Beguelin W., Flaque M.C., Cayrol F., Rivas M.A., Tkach M., Charreau E.H., Schillaci R., Elizalde P.V. Cancer - Interactions investigated in the context of cancer RE-CH-IP experiment patriciaelizalde@ibyme.conicet.uba.ar, cproietti@ibyme.conicet.uba.ar Steroids (0039-128X) 2011 Only protein-protein interactions imex curation proietti-2011-4 Novel role of signal transducer and activator of transcription 3 as a progesterone receptor coactivator in breast cancer. human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Proietti C.J., Beguelin W., Flaque M.C., Cayrol F., Rivas M.A., Tkach M., Charreau E.H., Schillaci R., Elizalde P.V. Cancer - Interactions investigated in the context of cancer RE-CH-IP experiment patriciaelizalde@ibyme.conicet.uba.ar, cproietti@ibyme.conicet.uba.ar Steroids (0039-128X) 2011 Only protein-protein interactions imex curation proietti-2011-1 Novel role of signal transducer and activator of transcription 3 as a progesterone receptor coactivator in breast cancer. mouse-c4hd Mouse C4HD - ER+and PR+ progestin (MPA) induced mammary adenocarcinoma cell line c4hd C4HD Mouse ER+and PR+ progestin (MPA) induced mammary adenocarcinoma cell line ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Proietti C.J., Beguelin W., Flaque M.C., Cayrol F., Rivas M.A., Tkach M., Charreau E.H., Schillaci R., Elizalde P.V. Cancer - Interactions investigated in the context of cancer RE-CH-IP experiment patriciaelizalde@ibyme.conicet.uba.ar, cproietti@ibyme.conicet.uba.ar Steroids (0039-128X) 2011 Only protein-protein interactions imex curation proietti-2011-5 Novel role of signal transducer and activator of transcription 3 as a progesterone receptor coactivator in breast cancer. human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Proietti C.J., Beguelin W., Flaque M.C., Cayrol F., Rivas M.A., Tkach M., Charreau E.H., Schillaci R., Elizalde P.V. Cancer - Interactions investigated in the context of cancer RE-CH-IP experiment patriciaelizalde@ibyme.conicet.uba.ar, cproietti@ibyme.conicet.uba.ar Steroids (0039-128X) 2011 Only protein-protein interactions imex curation proietti-2011-3 Novel role of signal transducer and activator of transcription 3 as a progesterone receptor coactivator in breast cancer. mouse-c4hd Mouse C4HD - ER+and PR+ progestin (MPA) induced mammary adenocarcinoma cell line c4hd C4HD Mouse ER+and PR+ progestin (MPA) induced mammary adenocarcinoma cell line ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Proietti C.J., Beguelin W., Flaque M.C., Cayrol F., Rivas M.A., Tkach M., Charreau E.H., Schillaci R., Elizalde P.V. Cancer - Interactions investigated in the context of cancer RE-CH-IP experiment patriciaelizalde@ibyme.conicet.uba.ar, cproietti@ibyme.conicet.uba.ar Steroids (0039-128X) 2011 Only protein-protein interactions imex curation proietti-2011-6 Novel role of signal transducer and activator of transcription 3 as a progesterone receptor coactivator in breast cancer. mouse-c4hd Mouse C4HD - ER+and PR+ progestin (MPA) induced mammary adenocarcinoma cell line c4hd C4HD Mouse ER+and PR+ progestin (MPA) induced mammary adenocarcinoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Proietti C.J., Beguelin W., Flaque M.C., Cayrol F., Rivas M.A., Tkach M., Charreau E.H., Schillaci R., Elizalde P.V. Cancer - Interactions investigated in the context of cancer RE-CH-IP experiment patriciaelizalde@ibyme.conicet.uba.ar, cproietti@ibyme.conicet.uba.ar Steroids (0039-128X) 2011 Only protein-protein interactions imex curation proietti-2011-7 Novel role of signal transducer and activator of transcription 3 as a progesterone receptor coactivator in breast cancer. mouse-c4hd Mouse C4HD - ER+and PR+ progestin (MPA) induced mammary adenocarcinoma cell line c4hd C4HD Mouse ER+and PR+ progestin (MPA) induced mammary adenocarcinoma cell line confocal microscopy confocal microscopy immunostaining immunostaining Proietti C.J., Beguelin W., Flaque M.C., Cayrol F., Rivas M.A., Tkach M., Charreau E.H., Schillaci R., Elizalde P.V. Cancer - Interactions investigated in the context of cancer RE-CH-IP experiment patriciaelizalde@ibyme.conicet.uba.ar, cproietti@ibyme.conicet.uba.ar Steroids (0039-128X) 2011 Only protein-protein interactions imex curation mmtv_promoter MMTV- Mouse Mammary Tumour Virus Promoter dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid mmtvc Mouse mammary tumor virus (strain C3H) prgr_human Progesterone receptor PGR NR3C3 Nuclear receptor subfamily 3 group C member 3 protein protein human Homo sapiens Human MTELKAKGPRAPHVAGGPPSPEVGSPLLCRPAAGPFPGSQTSDTLPEVSAIPISLDGLLFPRPCQGQDPSDEKTQDQQSLSDVEGAYSRAEATRGAGGSSSSPPEKDSGLLDSVLDTLLAPSGPGQSQPSPPACEVTSSWCLFGPELPEDPPAAPATQRVLSPLMSRSGCKVGDSSGTAAAHKVLPRGLSPARQLLLPASESPHWSGAPVKPSPQAAAVEVEEEDGSESEESAGPLLKGKPRALGGAAAGGGAAAVPPGAAAGGVALVPKEDSRFSAPRVALVEQDAPMAPGRSPLATTVMDFIHVPILPLNHALLAARTRQLLEDESYDGGAGAASAFAPPRSSPCASSTPVAVGDFPDCAYPPDAEPKDDAYPLYSDFQPPALKIKEEEEGAEASARSPRSYLVAGANPAAFPDFPLGPPPPLPPRATPSRPGEAAVTAAPASASVSSASSSGSTLECILYKAEGAPPQQGPFAPPPCKAPGASGCLLPRDGLPSTSASAAAAGAAPALYPALGLNGLPQLGYQAAVLKEGLPQVYPPYLNYLRPDSEASQSPQYSFESLPQKICLICGDEASGCHYGVLTCGSCKVFFKRAMEGQHNYLCAGRNDCIVDKIRRKNCPACRLRKCCQAGMVLGGRKFKKFNKVRVVRALDAVALPQPVGVPNESQALSQRFTFSPGQDIQLIPPLINLLMSIEPDVIYAGHDNTKPDTSSSLLTSLNQLGERQLLSVVKWSKSLPGFRNLHIDDQITLIQYSWMSLMVFGLGWRSYKHVSGQMLYFAPDLILNEQRMKESSFYSLCLTMWQIPQEFVKLQVSQEEFLCMKVLLLLNTIPLEGLRSQTQFEEMRSSYIRELIKAIGLRQKGVVSSSQRFYQLTKLLDNLHDLVKQLHLYCLNTFIQSRALSVEFPEMMSEVIAAQLPKILAGMVKPLLFHKK stat3_human Signal transducer and activator of transcription 3 Acute-phase response factor STAT3 APRF protein protein human Homo sapiens Human MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM 6C00632211C8012D cdkn1a_human_gene Human CDKN1A gene gene gene human Homo sapiens Human sp1_human Transcription factor Sp1 SP1 TSFP1 protein protein human Homo sapiens Human MSDQDHSMDEMTAVVKIEKGVGGNNGGNGNGGGAFSQARSSSTGSSSSTGGGGQESQPSPLALLAATCSRIESPNENSNNSQGPSQSGGTGELDLTATQLSQGANGWQIISSSSGATPTSKEQSGSSTNGSNGSESSKNRTVSGGQYVVAAAPNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQDGSGQIQIIPGANQQIITNRGSGGNIIAAMPNLLQQAVPLQGLANNVLSGQTQYVTNVPVALNGNITLLPVNSVSAATLTPSSQAVTISSSGSQESGSQPVTSGTTISSASLVSSQASSSSFFTNANSYSTTTTTSNMGIMNFTTSGSSGTNSQGQTPQRVSGLQGSDALNIQQNQTSGGSLQAGQQKEGEQNQQTQQQQILIQPQLVQGGQALQALQAAPLSGQTFTTQAISQETLQNLQLQAVPNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQTSSSNTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHPIQGLPLAIANAPGDHGAQLGLHGAGGDGIHDDTAGGEEGENSPDAQPQAGRRTRREACTCPYCKDSEGRGSGDPGKKKQHICHIQGCGKVYGKTSHLRAHLRWHTGERPFMCTWSYCGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLSKHIKTHQNKKGGPGVALSVGTLPLDSGAGSEGSGTATPSALITTNMVAMEAICPEGIARLANSGINVMQVADLQSINISGNGF b2cl1_mouse_gene Mouse Bcl2l1/BCL-X gene gene gene mouse Mus musculus Mouse prgr_mouse Progesterone receptor Pgr Nr3c3 Pr Nuclear receptor subfamily 3 group C member 3 protein protein mouse Mus musculus Mouse MTELQAKDPQVLHTSGASPSPPHIGSPLLARLDSGPFQGSQHSDVSSVVSPIPISLDGLLFPRSCRGPELPDGKTGDQQSLSDVEGAFSGVEATHREGGRNSRAPEKDSRLLDSVLDSLLTPSGTEQSHASPPACEAITSWCLFGPELPEDPRSVPATKGLLSPLMSRPEIKAGDSSGTGAGQKVLPKGLSPPRQLLLPTSGSAHWPGAGVKPSPQPAAGEVEEDSGLETEGSAAPLLKSKPRALEGTGSGGGVAANAASAAPGGVTLVPKEDSRFSAPRVSLEQDSPIAPGRSPLATTVVDFIHVPILPLNHALLAARTRQLLEGDSYDGGATAQGPFAPPRGSPSAPSPPVPCGDFPDCTYPLEGDPKEDVFPLYGDFQTPGLKIKEEEEGADAAVRSPRPYLSAGASSSTFPDFPLAPAPQRAPSSRPGEAAVAGGPSSAAVSPASSSGSALECILYKAEGAPPTQGSFAPLPCKPPAAGSCLLPRDSLPAAPATAAAPAIYQPLGLNGLPQLGYQAAVLKDSLPQVYPPYLNYLRPDSEASQSPQYGFDSLPQKICLICGDEASGCHYGVLTCGSCKVFFKRAMEGQHNYLCAGRNDCIVDKIRRKNCPACRLRKCCQAGMVLGGRKFKKFNKVRVMRTLDGVALPQSVGLPNESQALGQRITFSPNQEIQLVPPLINLLMSIEPDVVYAGHDNTKPDTSSSLLTSLNQLGERQLLSVVKWSKSLPGFRNLHIDDQITLIQYSWMSLMVFGLGWRSYKHVSGQMLYFAPDLILNEQRMKELSFYSLCLTMWQIPQEFVKLQVTHEEFLCMKVLLLLNTIPLEGLRSQSQFEEMRSSYIRELIKAIGLRQKGVVPSSQRFYQLTKLLDSLHDLVKQLHLYCLNTFIQSRTLAVEFPEMMSEVIAAQLPKILAGMVKPLLFHKK stat3_mouse Signal transducer and activator of transcription 3 Acute-phase response factor Stat3 Aprf protein protein mouse Mus musculus Mouse MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMDLTSECATSPM pgr-b2cl1-1 1308930 n/a 1308937 unspecified role unspecified role prey prey hormone response elements binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1308938 unspecified role unspecified role bait bait association association false false false Fig 1 C/D MPA - Medroxyprogesterone acetate - a synthetic variant of human progesterone. pgr-mmtv 1308930 n/a 1308937 unspecified role unspecified role prey prey hormone response elements binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1308939 unspecified role unspecified role bait bait association association false false false Fig 1 C/D MPA - Medroxyprogesterone acetate - a synthetic variant of human progesterone. pgr-cdkn1a 1308930 n/a 1308938 unspecified role unspecified role bait bait n/a 1308940 unspecified role unspecified role prey prey sp1_response_element binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 6B MPA sp1-cdkn1a-1 1308930 n/a 1308940 unspecified role unspecified role prey prey sp1_response_element binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1308941 unspecified role unspecified role bait bait association association false false false Fig 6B MPA sp1-cdkn1a-2 1308930 n/a 1308940 unspecified role unspecified role prey prey sp1_response_element binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1308939 unspecified role unspecified role bait bait association association false false false Fig 6B MPA stat3-mmtv 1308931 n/a 1308939 unspecified role unspecified role bait bait n/a 1308938 unspecified role unspecified role prey prey n/a 1308937 unspecified role unspecified role prey prey hormone response elements binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 1 G/H MPA stat3-cdkn1a 1308931 n/a 1308940 unspecified role unspecified role prey prey sp1_response_element binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1308941 unspecified role unspecified role prey prey n/a 1308939 unspecified role unspecified role bait bait association association false false false Fig 6D-1 MPA stat3-cdkn1a-1 1308931 n/a 1308940 unspecified role unspecified role prey prey sp1_response_element binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1308941 unspecified role unspecified role prey prey n/a 1308938 unspecified role unspecified role bait bait association association false false false Fig 6D-2 MPA pgr-b2cl1 1308932 n/a 1308942 unspecified role unspecified role prey prey promoter_xp4_region (with 2 progesterone response elements) binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1308943 unspecified role unspecified role bait bait association association false false false Fig 1 A/B MPA - Medroxyprogesterone acetate - a synthetic variant of human progesterone. pgr-stat3 1308932 n/a 1308944 unspecified role unspecified role bait bait n/a 1308942 unspecified role unspecified role prey prey promoter_xp4_region (with 2 progesterone response elements) binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 1 A/B MPA - Medroxyprogesterone acetate - a synthetic variant of human progesterone. stat3-pgr 1308933 n/a 1308938 unspecified role unspecified role prey prey p_ser294 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false n/a 1308939 unspecified role unspecified role bait bait p_tyr705 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 2A MPA sp1-pgr 1308933 n/a 1308941 unspecified role unspecified role bait bait n/a 1308939 unspecified role unspecified role prey prey n/a 1308938 unspecified role unspecified role prey prey association association false false false Fig 6A MPA - Medroxyprogesterone acetate - a synthetic variant of human progesterone. RU486 (Mifepristone) -a progesterone receptor antagonist. stat3-b2cl1 1308934 n/a 1308944 unspecified role unspecified role bait bait n/a 1308943 unspecified role unspecified role prey prey n/a 1308942 unspecified role unspecified role prey prey promoter_xp4_region (with 2 progesterone response elements) binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 1 E/F MPA - Medroxyprogesterone acetate - a synthetic variant of human progesterone. stat3-b2cl1-1 1308935 n/a 1308943 unspecified role unspecified role prey prey p_294ser opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain false n/a 1308944 unspecified role unspecified role bait bait p_tyr705 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 2A MPA pgr-stat3-1 1308936 n/a 1308944 unspecified role unspecified role neutral component neutral component n/a 1308943 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 2B MPA