IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ beguelin-2010-5 Progesterone receptor induces ErbB-2 nuclear translocation to promote breast cancer growth via a novel transcriptional effect: ErbB-2 function as a coactivator of Stat3. human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Beguelin W., Diaz Flaque M.C., Proietti C.J., Cayrol F., Rivas M.A., Tkach M., Rosemblit C., Tocci J.M., Charreau E.H., Schillaci R., Elizalde P.V. Cancer - Interactions investigated in the context of cancer Interactions involving H3 have not been added as the identity of the histone molecule is not known. Interactions involving H3 have not been included due to non-specificity of the antibody used for ch-IP. Elizalde@dna.uba.ar Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation beguelin-2010-7 Progesterone receptor induces ErbB-2 nuclear translocation to promote breast cancer growth via a novel transcriptional effect: ErbB-2 function as a coactivator of Stat3. human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Beguelin W., Diaz Flaque M.C., Proietti C.J., Cayrol F., Rivas M.A., Tkach M., Rosemblit C., Tocci J.M., Charreau E.H., Schillaci R., Elizalde P.V. Cancer - Interactions investigated in the context of cancer RE-ch-Ip experiment Interactions involving H3 have not been included due to non-specificity of the antibody used for ch-IP. Elizalde@dna.uba.ar Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation beguelin-2010-4 Progesterone receptor induces ErbB-2 nuclear translocation to promote breast cancer growth via a novel transcriptional effect: ErbB-2 function as a coactivator of Stat3. mouse-c4hd Mouse C4HD - ER+and PR+ progestin (MPA) induced mammary adenocarcinoma cell line c4hd C4HD Mouse ER+and PR+ progestin (MPA) induced mammary adenocarcinoma cell line ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Beguelin W., Diaz Flaque M.C., Proietti C.J., Cayrol F., Rivas M.A., Tkach M., Rosemblit C., Tocci J.M., Charreau E.H., Schillaci R., Elizalde P.V. Cancer - Interactions investigated in the context of cancer Interactions involving H3 have not been included due to non-specificity of the antibody used for ch-IP. Elizalde@dna.uba.ar Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation beguelin-2010-3 Progesterone receptor induces ErbB-2 nuclear translocation to promote breast cancer growth via a novel transcriptional effect: ErbB-2 function as a coactivator of Stat3. mouse-c4hd Mouse C4HD - ER+and PR+ progestin (MPA) induced mammary adenocarcinoma cell line c4hd C4HD Mouse ER+and PR+ progestin (MPA) induced mammary adenocarcinoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Beguelin W., Diaz Flaque M.C., Proietti C.J., Cayrol F., Rivas M.A., Tkach M., Rosemblit C., Tocci J.M., Charreau E.H., Schillaci R., Elizalde P.V. Cancer - Interactions investigated in the context of cancer Interactions involving H3 have not been included due to non-specificity of the antibody used for ch-IP. Elizalde@dna.uba.ar Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation beguelin-2010-6 Progesterone receptor induces ErbB-2 nuclear translocation to promote breast cancer growth via a novel transcriptional effect: ErbB-2 function as a coactivator of Stat3. mouse-c4hd Mouse C4HD - ER+and PR+ progestin (MPA) induced mammary adenocarcinoma cell line c4hd C4HD Mouse ER+and PR+ progestin (MPA) induced mammary adenocarcinoma cell line ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Beguelin W., Diaz Flaque M.C., Proietti C.J., Cayrol F., Rivas M.A., Tkach M., Rosemblit C., Tocci J.M., Charreau E.H., Schillaci R., Elizalde P.V. Cancer - Interactions investigated in the context of cancer RE-ChIp experiment Interactions involving H3 have not been included due to non-specificity of the antibody used for ch-IP. Elizalde@dna.uba.ar Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation beguelin-2010-1 Progesterone receptor induces ErbB-2 nuclear translocation to promote breast cancer growth via a novel transcriptional effect: ErbB-2 function as a coactivator of Stat3. mouse-c4hd Mouse C4HD - ER+and PR+ progestin (MPA) induced mammary adenocarcinoma cell line c4hd C4HD Mouse ER+and PR+ progestin (MPA) induced mammary adenocarcinoma cell line confocal microscopy confocal microscopy immunostaining immunostaining Beguelin W., Diaz Flaque M.C., Proietti C.J., Cayrol F., Rivas M.A., Tkach M., Rosemblit C., Tocci J.M., Charreau E.H., Schillaci R., Elizalde P.V. Cancer - Interactions investigated in the context of cancer Interactions involving H3 have not been included due to non-specificity of the antibody used for ch-IP. Elizalde@dna.uba.ar Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation beguelin-2010-2 Progesterone receptor induces ErbB-2 nuclear translocation to promote breast cancer growth via a novel transcriptional effect: ErbB-2 function as a coactivator of Stat3. human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour confocal microscopy confocal microscopy immunostaining immunostaining Beguelin W., Diaz Flaque M.C., Proietti C.J., Cayrol F., Rivas M.A., Tkach M., Rosemblit C., Tocci J.M., Charreau E.H., Schillaci R., Elizalde P.V. Cancer - Interactions investigated in the context of cancer Interactions involving H3 have not been included due to non-specificity of the antibody used for ch-IP. Elizalde@dna.uba.ar Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation beguelin-2010-8 Progesterone receptor induces ErbB-2 nuclear translocation to promote breast cancer growth via a novel transcriptional effect: ErbB-2 function as a coactivator of Stat3. human-t47d-y Human T47D-Y (T47DY) -PR negative variant of T47D cell line t47d-y T47D-Y (T47DY) -PR negative variant of T47D cell line ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Beguelin W., Diaz Flaque M.C., Proietti C.J., Cayrol F., Rivas M.A., Tkach M., Rosemblit C., Tocci J.M., Charreau E.H., Schillaci R., Elizalde P.V. Cancer - Interactions investigated in the context of cancer T47D-Y cells (PR-null T47D cells) were transfected with mutant PR - C587A Interactions involving H3 have not been included due to non-specificity of the antibody used for ch-IP. Elizalde@dna.uba.ar Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation stat3_human Signal transducer and activator of transcription 3 Acute-phase response factor STAT3 APRF protein protein human Homo sapiens Human MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM 6C00632211C8012D ccnd1_human_gene Human Cyclin D1 gene gene gene human Homo sapiens Human erbb2_human Receptor tyrosine-protein kinase erbB-2 p185erbB2 MLN19 Proto-oncogene c-ErbB-2 Tyrosine kinase-type cell surface receptor HER2 Proto-oncogene Neu ERBB2 HER2 NEU NGL Metastatic lymph node gene 19 protein protein protein human Homo sapiens Human MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLREVRAVTSANIQEFAGCKKIFGSLAFLPESFDGDPASNTAPLQPEQLQVFETLEEITGYLYISAWPDSLPDLSVFQNLQVIRGRILHNGAYSLTLQGLGISWLGLRSLRELGSGLALIHHNTHLCFVHTVPWDQLFRNPHQALLHTANRPEDECVGEGLACHQLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVLQGLPREYVNARHCLPCHPECQPQNGSVTCFGPEADQCVACAHYKDPPFCVARCPSGVKPDLSYMPIWKFPDEEGACQPCPINCTHSCVDLDDKGCPAEQRASPLTSIISAVVGILLVVVLGVVFGILIKRRQQKIRKYTMRRLLQETELVEPLTPSGAMPNQAQMRILKETELRKVKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVRENRGRLGSQDLLNWCMQIAKGMSYLEDVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPASPLDSTFYRSLLEDDDMGDLVDAEEYLVPQQGFFCPDPAPGAGGMVHHRHRSSSTRSGGGDLTLGLEPSEEEAPRSPLAPSEGAGSDVFDGDLGMGAAKGLQSLPTHDPSPLQRYSEDPTVPLPSETDGYVAPLTCSPQPEYVNQPDVRPQPPSPREGPLPAARPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLTPQGGAAPQPHPPPAFSPAFDNLYYWDQDPPERGAPPSTFKGTPTAENPEYLGLDVPV prgr_human Progesterone receptor PGR NR3C3 Nuclear receptor subfamily 3 group C member 3 protein protein human Homo sapiens Human MTELKAKGPRAPHVAGGPPSPEVGSPLLCRPAAGPFPGSQTSDTLPEVSAIPISLDGLLFPRPCQGQDPSDEKTQDQQSLSDVEGAYSRAEATRGAGGSSSSPPEKDSGLLDSVLDTLLAPSGPGQSQPSPPACEVTSSWCLFGPELPEDPPAAPATQRVLSPLMSRSGCKVGDSSGTAAAHKVLPRGLSPARQLLLPASESPHWSGAPVKPSPQAAAVEVEEEDGSESEESAGPLLKGKPRALGGAAAGGGAAAVPPGAAAGGVALVPKEDSRFSAPRVALVEQDAPMAPGRSPLATTVMDFIHVPILPLNHALLAARTRQLLEDESYDGGAGAASAFAPPRSSPCASSTPVAVGDFPDCAYPPDAEPKDDAYPLYSDFQPPALKIKEEEEGAEASARSPRSYLVAGANPAAFPDFPLGPPPPLPPRATPSRPGEAAVTAAPASASVSSASSSGSTLECILYKAEGAPPQQGPFAPPPCKAPGASGCLLPRDGLPSTSASAAAAGAAPALYPALGLNGLPQLGYQAAVLKEGLPQVYPPYLNYLRPDSEASQSPQYSFESLPQKICLICGDEASGCHYGVLTCGSCKVFFKRAMEGQHNYLCAGRNDCIVDKIRRKNCPACRLRKCCQAGMVLGGRKFKKFNKVRVVRALDAVALPQPVGVPNESQALSQRFTFSPGQDIQLIPPLINLLMSIEPDVIYAGHDNTKPDTSSSLLTSLNQLGERQLLSVVKWSKSLPGFRNLHIDDQITLIQYSWMSLMVFGLGWRSYKHVSGQMLYFAPDLILNEQRMKESSFYSLCLTMWQIPQEFVKLQVSQEEFLCMKVLLLLNTIPLEGLRSQTQFEEMRSSYIRELIKAIGLRQKGVVSSSQRFYQLTKLLDNLHDLVKQLHLYCLNTFIQSRALSVEFPEMMSEVIAAQLPKILAGMVKPLLFHKK cbp_human CREB-binding protein CREBBP CBP Histone lysine acetyltransferase CREBBP Protein-lysine acetyltransferase CREBBP protein protein human Homo sapiens Human MAENLLDGPPNPKRAKLSSPGFSANDSTDFGSLFDLENDLPDELIPNGGELGLLNSGNLVPDAASKHKQLSELLRGGSGSSINPGIGNVSASSPVQQGLGGQAQGQPNSANMASLSAMGKSPLSQGDSSAPSLPKQAASTSGPTPAASQALNPQAQKQVGLATSSPATSQTGPGICMNANFNQTHPGLLNSNSGHSLINQASQGQAQVMNGSLGAAGRGRGAGMPYPTPAMQGASSSVLAETLTQVSPQMTGHAGLNTAQAGGMAKMGITGNTSPFGQPFSQAGGQPMGATGVNPQLASKQSMVNSLPTFPTDIKNTSVTNVPNMSQMQTSVGIVPTQAIATGPTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRACSLPHCRTMKNVLNHMTHCQAGKACQVAHCASSRQIISHWKNCTRHDCPVCLPLKNASDKRNQQTILGSPASGIQNTIGSVGTGQQNATSLSNPNPIDPSSMQRAYAALGLPYMNQPQTQLQPQVPGQQPAQPQTHQQMRTLNPLGNNPMNIPAGGITTDQQPPNLISESALPTSLGATNPLMNDGSNSGNIGTLSTIPTAAPPSSTGVRKGWHEHVTQDLRSHLVHKLVQAIFPTPDPAALKDRRMENLVAYAKKVEGDMYESANSRDEYYHLLAEKIYKIQKELEEKRRSRLHKQGILGNQPALPAPGAQPPVIPQAQPVRPPNGPLSLPVNRMQVSQGMNSFNPMSLGNVQLPQAPMGPRAASPMNHSVQMNSMGSVPGMAISPSRMPQPPNMMGAHTNNMMAQAPAQSQFLPQNQFPSSSGAMSVGMGQPPAQTGVSQGQVPGAALPNPLNMLGPQASQLPCPPVTQSPLHPTPPPASTAAGMPSLQHTTPPGMTPPQPAAPTQPSTPVSSSGQTPTPTPGSVPSATQTQSTPTVQAAAQAQVTPQPQTPVQPPSVATPQSSQQQPTPVHAQPPGTPLSQAAASIDNRVPTPSSVASAETNSQQPGPDVPVLEMKTETQAEDTEPDPGESKGEPRSEMMEEDLQGASQVKEETDIAEQKSEPMEVDEKKPEVKVEVKEEEESSSNGTASQSTSPSQPRKKIFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDIVKNPMDLSTIKRKLDTGQYQEPWQYVDDVWLMFNNAWLYNRKTSRVYKFCSKLAEVFEQEIDPVMQSLGYCCGRKYEFSPQTLCCYGKQLCTIPRDAAYYSYQNRYHFCEKCFTEIQGENVTLGDDPSQPQTTISKDQFEKKKNDTLDPEPFVDCKECGRKMHQICVLHYDIIWPSGFVCDNCLKKTGRPRKENKFSAKRLQTTRLGNHLEDRVNKFLRRQNHPEAGEVFVRVVASSDKTVEVKPGMKSRFVDSGEMSESFPYRTKALFAFEEIDGVDVCFFGMHVQEYGSDCPPPNTRRVYISYLDSIHFFRPRCLRTAVYHEILIGYLEYVKKLGYVTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQEWYKKMLDKAFAERIIHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLEESIKELEQEEEERKKEESTAASETTEGSQGDSKNAKKKNNKKTNKNKSSISRANKKKPSMPNVSNDLSQKLYATMEKHKEVFFVIHLHAGPVINTLPPIVDPDPLLSCDLMDGRDAFLTLARDKHWEFSSLRRSKWSTLCMLVELHTQGQDRFVYTCNECKHHVETRWHCTVCEDYDLCINCYNTKSHAHKMVKWGLGLDDEGSSQGEPQSKSPQESRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRVVQHTKGCKRKTNGGCPVCKQLIALCCYHAKHCQENKCPVPFCLNIKHKLRQQQIQHRLQQAQLMRRRMATMNTRNVPQQSLPSPTSAPPGTPTQQPSTPQTPQPPAQPQPSPVSMSPAGFPSVARTQPPTTVSTGKPTSQVPAPPPPAQPPPAAVEAARQIEREAQQQQHLYRVNINNSMPPGRTGMGTPGSQMAPVSLNVPRPNQVSGPVMPSMPPGQWQQAPLPQQQPMPGLPRPVISMQAQAAVAGPRMPSVQPPRSISPSALQDLLRTLKSPSSPQQQQQVLNILKSNPQLMAAFIKQRTAKYVANQPGMQPQPGLQSQPGMQPQPGMHQQPSLQNLNAMQAGVPRPGVPPQQQAMGGLNPQGQALNIMNPGHNPNMASMNPQYREMLRRQLLQQQQQQQQQQQQQQQQQQGSAGMAGGMAGHGQFQQPQGPGGYPPAMQQQQRMQQHLPLQGSSMGQMAAQMGQLGQMGQPGLGADSTPNIQQALQQRILQQQQMKQQIGSPGQPNPMSPQQHMLSGQPQASHLPGQQIATSLSNQVRSPAPVQSPRPQSQPPHSSPSPRIQPQPSPHHVSPQTGSPHPGLAVTMASSIDQGHLGNPEQSAMLPQLNTPSRSALSSELSLVGDTTGDTLEKFVEGL ep300_human Histone acetyltransferase p300 Histone butyryltransferase p300 Histone crotonyltransferase p300 Protein propionyltransferase p300 EP300 P300 E1A-associated protein p300 Protein 2-hydroxyisobutyryltransferase p300 Protein lactyltransferas p300 protein protein human Homo sapiens Human MAENVVEPGPPSAKRPKLSSPALSASASDGTDFGSLFDLEHDLPDELINSTELGLTNGGDINQLQTSLGMVQDAASKHKQLSELLRSGSSPNLNMGVGGPGQVMASQAQQSSPGLGLINSMVKSPMTQAGLTSPNMGMGTSGPNQGPTQSTGMMNSPVNQPAMGMNTGMNAGMNPGMLAAGNGQGIMPNQVMNGSIGAGRGRQNMQYPNPGMGSAGNLLTEPLQQGSPQMGGQTGLRGPQPLKMGMMNNPNPYGSPYTQNPGQQIGASGLGLQIQTKTVLSNNLSPFAMDKKAVPGGGMPNMGQQPAPQVQQPGLVTPVAQGMGSGAHTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRQCNLPHCRTMKNVLNHMTHCQSGKSCQVAHCASSRQIISHWKNCTRHDCPVCLPLKNAGDKRNQQPILTGAPVGLGNPSSLGVGQQSAPNLSTVSQIDPSSIERAYAALGLPYQVNQMPTQPQVQAKNQQNQQPGQSPQGMRPMSNMSASPMGVNGGVGVQTPSLLSDSMLHSAINSQNPMMSENASVPSLGPMPTAAQPSTTGIRKQWHEDITQDLRNHLVHKLVQAIFPTPDPAALKDRRMENLVAYARKVEGDMYESANNRAEYYHLLAEKIYKIQKELEEKRRTRLQKQNMLPNAAGMVPVSMNPGPNMGQPQPGMTSNGPLPDPSMIRGSVPNQMMPRITPQSGLNQFGQMSMAQPPIVPRQTPPLQHHGQLAQPGALNPPMGYGPRMQQPSNQGQFLPQTQFPSQGMNVTNIPLAPSSGQAPVSQAQMSSSSCPVNSPIMPPGSQGSHIHCPQLPQPALHQNSPSPVPSRTPTPHHTPPSIGAQQPPATTIPAPVPTPPAMPPGPQSQALHPPPRQTPTPPTTQLPQQVQPSLPAAPSADQPQQQPRSQQSTAASVPTPTAPLLPPQPATPLSQPAVSIEGQVSNPPSTSSTEVNSQAIAEKQPSQEVKMEAKMEVDQPEPADTQPEDISESKVEDCKMESTETEERSTELKTEIKEEEDQPSTSATQSSPAPGQSKKKIFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDIVKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQEIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQGESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAGFVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHASDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPPPNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLSRGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDLMDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQDRFVYTCNECKHHVETRWHCTVCEDYDLCITCYNTKNHDHKMEKLGLGLDDESNNQQAAATQSPGDSRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRVVQHTKGCKRKTNGGCPICKQLIALCCYHAKHCQENKCPVPFCLNIKQKLRQQQLQHRLQQAQMLRRRMASMQRTGVVGQQQGLPSPTPATPTTPTGQQPTTPQTPQPTSQPQPTPPNSMPPYLPRTQAAGPVSQGKAAGQVTPPTPPQTAQPPLPGPPPAAVEMAMQIQRAAETQRQMAHVQIFQRPIQHQMPPMTPMAPMGMNPPPMTRGPSGHLEPGMGPTGMQQQPPWSQGGLPQPQQLQSGMPRPAMMSVAQHGQPLNMAPQPGLGQVGISPLKPGTVSQQALQNLLRTLRSPSSPLQQQQVLSILHANPQLLAAFIKQRAAKYANSNPQPIPGQPGMPQGQPGLQPPTMPGQQGVHSNPAMQNMNPMQAGVQRAGLPQQQPQQQLQPPMGGMSPQAQQMNMNHNTMPSQFRDILRRQQMMQQQQQQGAGPGIGPGMANHNQFQQPQGVGYPPQQQQRMQHHMQQMQQGNMGQIGQLPQALGAEAGASLQAYQQRLLQQQMGSPVQPNPMSPQQHMLPNQAQSPHLQGQQIPNSLSNQVRSPQPVPSPRPQSQPPHSSPSPRMQPQPSPHHVSPQTSSPHPGLVAAQANPMEQGHFASPDQNSMLSQLASNPGMANLHGASATDLGLSTDNSDLNSNLSQSTLDIH h4_human Histone H4 H4-16 H4C1 H4C11 H4C12 H4C13 H4C14 H4C15 H4C2 H4C3 H4C4 H4C5 H4C6 H4C8 H4C9 H4/A H4/B H4/C H4/D H4/E H4/G H4/H H4/I H4/J H4/K H4/M H4/N H4/O H4F2 H4FA H4FB H4FC H4FD H4FE H4FG H4FH H4FI H4FJ H4FK H4FM H4FN H4FO HIST1H4A HIST1H4B HIST1H4C HIST1H4D HIST1H4E HIST1H4F HIST1H4H HIST1H4I HIST1H4J HIST1H4K HIST1H4L HIST2H4 HIST2H4A HIST2H4B HIST4H4 protein protein human Homo sapiens Human MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG mouse_ccnd1_gene Ccnd1/G1/S-specific cyclin-D1 Mouse Gene gene gene mouse Mus musculus Mouse stat3_mouse Signal transducer and activator of transcription 3 Acute-phase response factor Stat3 Aprf protein protein mouse Mus musculus Mouse MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMDLTSECATSPM erbb2_mouse Receptor tyrosine-protein kinase erbB-2 Erbb2 Kiaa3023 Neu Proto-oncogene Neu Proto-oncogene c-ErbB-2 p185erbB2 protein protein mouse Mus musculus Mouse MELAAWCRWGFLLALLSPGAAGTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPANASLSFLQDIQEVQGYMLIAHNRVKHVPLQRLRIVRGTQLFEDKYALAVLDNRDPLDNVTTAAPGRTPEGLRELQLRSLTEILKGGVLIRGNPQLCYQDMVLWKDVLRKNNQLAPVDMDTNRSRACPPCAPTCKDNHCWGESPEDCQILTGTICTSGCARCKGRLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALITYNTDTFESMLNPEGRYTFGASCVTTCPYNYLSTEVGSCTLVCPPNNQEVTAEDGTQRCEKCSKPCAGVCYGLGMEHLRGARAITSDNIQEFAGCKKIFGSLAFLPESFDGNPSSGVAPLKPEHLQVFETLEEITGYLYISAWPESFQDLSVFQNLRVIRGRILHDGAYSLTLQGLGIHSLGLRSLRELGSGLALIHRNTHLCFVNTVPWDQLFRNPHQALLHSGNRPEEACGLEGLVCNSLCARGHCWGPGPTQCVNCSQFLRGQECVEECRVWKGLPREYVRGKHCLPCHPECQPQNSSETCYGSEADQCEACAHYKDSSSCVARCPSGVKPDLSYMPIWKYPDEEGICQPCPINCTHSCVDLDERGCPAEQRASPVTFIIATVVGVLLFLIIVVVIGILIKRRRQKIRKYTMRRLLQETELVEPLTPSGAVPNQAQMRILKETELRKLKVLGSGAFGTVYKGIWIPDGENVKIPVAIKVLRENTSPKANKEILDEAYVMAGVGSPYVSRLLGICLTSTVQLVTQLMPYGCLLDHVREHRGRLGSQDLLNWCVQIAKGMSYLEEVRLVHRDLAARNVLVKSPNHVKITDFGLARLLDIDETEYHADGGKVPIKWMALESILRRRFTHQSDVWSYGVTVWELMTFGAKPYDGIPAREIPDLLEKGERLPQPPICTIDVYMIMVKCWMIDSECRPRFRELVSEFSRMARDPQRFVVIQNEDLGPSSPMDSTFYRSLLEDDDMGELVDAEEYLVPQQGFFSPDPALGTGSTAHRRHRSSSARSGGGELTLGLEPSEEEPPRSPLAPSEGAGSDVFDGDLAVGVTKGLQSLSPHDLSPLQRYSEDPTLPLPPETDGYVAPLACSPQPEYVNQPEVRPQSPLTPEGPPPPIRPAGATLERPKTLSPGKNGVVKDVFAFGGAVENPEYLAPRAGTASQPHPSPAFSPAFDNLYYWDQNSSEQGPPPSTFEGTPTAENPEYLGLDVPV prgr_mouse Progesterone receptor Pgr Nr3c3 Pr Nuclear receptor subfamily 3 group C member 3 protein protein mouse Mus musculus Mouse MTELQAKDPQVLHTSGASPSPPHIGSPLLARLDSGPFQGSQHSDVSSVVSPIPISLDGLLFPRSCRGPELPDGKTGDQQSLSDVEGAFSGVEATHREGGRNSRAPEKDSRLLDSVLDSLLTPSGTEQSHASPPACEAITSWCLFGPELPEDPRSVPATKGLLSPLMSRPEIKAGDSSGTGAGQKVLPKGLSPPRQLLLPTSGSAHWPGAGVKPSPQPAAGEVEEDSGLETEGSAAPLLKSKPRALEGTGSGGGVAANAASAAPGGVTLVPKEDSRFSAPRVSLEQDSPIAPGRSPLATTVVDFIHVPILPLNHALLAARTRQLLEGDSYDGGATAQGPFAPPRGSPSAPSPPVPCGDFPDCTYPLEGDPKEDVFPLYGDFQTPGLKIKEEEEGADAAVRSPRPYLSAGASSSTFPDFPLAPAPQRAPSSRPGEAAVAGGPSSAAVSPASSSGSALECILYKAEGAPPTQGSFAPLPCKPPAAGSCLLPRDSLPAAPATAAAPAIYQPLGLNGLPQLGYQAAVLKDSLPQVYPPYLNYLRPDSEASQSPQYGFDSLPQKICLICGDEASGCHYGVLTCGSCKVFFKRAMEGQHNYLCAGRNDCIVDKIRRKNCPACRLRKCCQAGMVLGGRKFKKFNKVRVMRTLDGVALPQSVGLPNESQALGQRITFSPNQEIQLVPPLINLLMSIEPDVVYAGHDNTKPDTSSSLLTSLNQLGERQLLSVVKWSKSLPGFRNLHIDDQITLIQYSWMSLMVFGLGWRSYKHVSGQMLYFAPDLILNEQRMKELSFYSLCLTMWQIPQEFVKLQVTHEEFLCMKVLLLLNTIPLEGLRSQSQFEEMRSSYIRELIKAIGLRQKGVVPSSQRFYQLTKLLDSLHDLVKQLHLYCLNTFIQSRTLAVEFPEMMSEVIAAQLPKILAGMVKPLLFHKK stat3-ccnd1 1309164 n/a 1309172 unspecified role unspecified role bait bait n/a 1309173 unspecified role unspecified role prey prey promoter_region containing_x1_gamma interferon-activated sequence binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 5A-1, 5C-bottom, 5C-middle. MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. In the absence of ErbB-2 recruitment (by use of a ERBB2 NLS deletion mutant), PR was not loaded at the GAS site at the cyclin D1 promoter. MPA-induced Stat3 binding at this site remained unaffected. erbb2-ccnd1 1309164 n/a 1309173 unspecified role unspecified role prey prey promoter_region containing_x1_gamma interferon-activated sequence binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1309174 unspecified role unspecified role bait bait association association false false false Fig 5A-2, 5C-bottom. MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. pgr-ccnd1 1309164 n/a 1309175 unspecified role unspecified role bait bait n/a 1309173 unspecified role unspecified role prey prey promoter_region containing_x1_gamma interferon-activated sequence binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 5A-3, 5C-bottom. MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. crebbp-ccnd1 1309164 n/a 1309176 unspecified role unspecified role bait bait n/a 1309173 unspecified role unspecified role prey prey promoter_region containing_x1_gamma interferon-activated sequence binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 5D-top and bottom-1. MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. In the absence of ErbB-2 recruitment (by use of a ERBB2 NLS deletion mutant), CBP, H4 and p300 were not loaded at the GAS site at the cyclin D1 promoter. ep300-ccnd1 1309164 n/a 1309177 unspecified role unspecified role bait bait n/a 1309173 unspecified role unspecified role prey prey promoter_region containing_x1_gamma interferon-activated sequence binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 5D-top and bottom.-2 MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. In the absence of ErbB-2 recruitment (by use of a ERBB2 NLS deletion mutant), CBP, H4 and p300 were not loaded at the GAS site at the cyclin D1 promoter. hist1h4a-ccnd1 1309164 n/a 1309178 unspecified role unspecified role bait bait region naclys N-acetylated L-lysine NAcLys undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1309173 unspecified role unspecified role prey prey promoter_region containing_x1_gamma interferon-activated sequence binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 5D-top and bottom-3. MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. In the absence of ErbB-2 recruitment (by use of a ERBB2 NLS deletion mutant), CBP, H4 and p300 were not loaded at the GAS site at the cyclin D1 promoter. erbb2-cdkn1a-2 1309165 n/a 1309172 unspecified role unspecified role prey prey n/a 1309175 unspecified role unspecified role bait bait n/a 1309173 unspecified role unspecified role prey prey promoter_region containing_x1_gamma interferon-activated sequence binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 5B-2 MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. erbb2-cdkn1a-3 1309165 n/a 1309173 unspecified role unspecified role prey prey promoter_region containing_x1_gamma interferon-activated sequence binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1309174 unspecified role unspecified role bait bait n/a 1309172 unspecified role unspecified role prey prey association association false false false Fig 5B-1 MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. erbb2-cdkn1a-4 1309165 n/a 1309173 unspecified role unspecified role prey prey promoter_region containing_x1_gamma interferon-activated sequence binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1309175 unspecified role unspecified role prey prey n/a 1309174 unspecified role unspecified role bait bait association association false false false Fig 5B-3 MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. pgr-cdkn1a-3 1309165 n/a 1309174 unspecified role unspecified role prey prey n/a 1309173 unspecified role unspecified role prey prey promoter_region containing_x1_gamma interferon-activated sequence binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1309175 unspecified role unspecified role bait bait association association false false false Fig 5B-4 MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. stat3-cdkn1a-2 1309166 n/a 1309179 unspecified role unspecified role prey prey promoter_region containing_x2_gamma interferon-activated sequences binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1309180 unspecified role unspecified role bait bait association association false false false Fig 5A-2, 7E. MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. The transfection of hErbB-2ΔNLS (Human ERBB2-NLS deletion mutant) in mouse C4HD cells resulted in no erbb2 binding at the cyclin D1 promoter , yet MPA-induced Stat3 binding at this site remained unaffected. erbb2-cdkn1a 1309166 n/a 1309179 unspecified role unspecified role prey prey promoter_region containing_x2_gamma interferon-activated sequences binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1309181 unspecified role unspecified role bait bait association association false false false Fig 5a-1, 7E. MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. The transfection of hErbB-2ΔNLS (Human ERBB2-NLS deletion mutant) in mouse C4HD cells resulted in no erbb2 binding at the cyclin D1 promoter , yet MPA-induced Stat3 binding at this site remained unaffected. pgr-cdkn1a-1 1309166 n/a 1309182 unspecified role unspecified role bait bait n/a 1309179 unspecified role unspecified role prey prey promoter_region containing_x2_gamma interferon-activated sequences binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig 5A-3 MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. erbb2-stat3-3 1309167 n/a 1309180 unspecified role unspecified role prey prey n/a 1309181 unspecified role unspecified role bait bait physical association physical association false false false Fig 2B MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. stat3-erbb2-2 1309167 n/a 1309180 unspecified role unspecified role bait bait n/a 1309181 unspecified role unspecified role prey prey physical association physical association false false false Fig 2B MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. erbb2-cdkn1a-1 1309168 n/a 1309179 unspecified role unspecified role prey prey promoter_region containing_x2_gamma interferon-activated sequences binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1309180 unspecified role unspecified role prey prey n/a 1309181 unspecified role unspecified role bait bait association association false false false Fig 5B-1 MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. pgr-cdkn1a-2 1309168 n/a 1309182 unspecified role unspecified role bait bait n/a 1309179 unspecified role unspecified role prey prey promoter_region containing_x2_gamma interferon-activated sequences binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1309180 unspecified role unspecified role prey prey association association false false false Fig 5B-2 MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. erbb2-stat3 1309169 n/a 1309180 unspecified role unspecified role neutral component neutral component n/a 1309181 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 2A MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. MPA treatment of C4HD and T47D cells resulted in a strong nuclear colocalization of ErbB-2 and Stat3. In the absence of MPA stimulation, the vast majority of ErbB-2 was localized in the cytoplasmic membrane. RU486-Mifepristone, a progesterone receptor antagonist. AG825-Tyrphostin, a selective inhibitor of ERBB2 autophosphorylation. erbb2-stat3-1 1309170 n/a 1309172 unspecified role unspecified role neutral component neutral component n/a 1309174 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig 2A MPA -Medroxyprogesterone acetate - a synthetic variant of human progesterone. MPA treatment of C4HD and T47D cells resulted in a strong nuclear colocalization of ErbB-2 and Stat3. In the absence of MPA stimulation, the vast majority of ErbB-2 was localized in the cytoplasmic membrane. RU486-Mifepristone, a progesterone receptor antagonist. AG825-Tyrphostin, a selective inhibitor of ERBB2 autophosphorylation. stat3-cdkn1a-3 1309171 n/a 1309172 unspecified role unspecified role bait bait n/a 1309173 unspecified role unspecified role prey prey association association false false false Fig 5C- top MPA while a clear Stat3 recruitment was observed upon the stimulation of T47D-Y-C587A-PR cells by MPA, C587A-PR (lacking a functional DNA binding domain) did not bind to this promoter, nor did ErbB-2.