IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ wang-2010d-1 Targeting mitochondrial glutaminase activity inhibits oncogenic transformation. in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Wang JB., Erickson JW., Fuji R., Ramachandran S., Gao P., Dinavahi R., Wilson KF., Ambrosio AL., Dias SM., Dang CV., Cerione RA. Cancer - Interactions investigated in the context of cancer Glutaminase activity was determined by incubating the reaction mixture (Recombinant GAC, varying dilutions of 968 diluted in DMSO, glutamine solution) for 1hr at 37 degrees C, stopping the reaction, and adding glutamate dehydrogenase to convert the glutamine produced to a-KGA. After incubation at room temperature for 45 min the absorbance at 340 nm was recorded for each sample. Insufficient information has been provided to include other interactions in this paper. rac1@cornell.edu Cancer Cell (1535-6108) 2010 wang-2010d-2 Targeting mitochondrial glutaminase activity inhibits oncogenic transformation. mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells pull down pull down anti tag western anti tag western blot anti tag western Wang JB., Erickson JW., Fuji R., Ramachandran S., Gao P., Dinavahi R., Wilson KF., Ambrosio AL., Dias SM., Dang CV., Cerione RA. Cancer - Interactions investigated in the context of cancer Glutaminase activity was determined by incubating the reaction mixture (Recombinant GAC, varying dilutions of 968 diluted in DMSO, glutamine solution) for 1hr at 37 degrees C, stopping the reaction, and adding glutamate dehydrogenase to convert the glutamine produced to a-KGA. After incubation at room temperature for 45 min the absorbance at 340 nm was recorded for each sample. Insufficient information has been provided to include other interactions in this paper. rac1@cornell.edu Cancer Cell (1535-6108) 2010 wang-2010d-3 Targeting mitochondrial glutaminase activity inhibits oncogenic transformation. mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells pull down pull down ms participant Identification by mass spectrometry ms participant Wang JB., Erickson JW., Fuji R., Ramachandran S., Gao P., Dinavahi R., Wilson KF., Ambrosio AL., Dias SM., Dang CV., Cerione RA. Cancer - Interactions investigated in the context of cancer Glutaminase activity was determined by incubating the reaction mixture (Recombinant GAC, varying dilutions of 968 diluted in DMSO, glutamine solution) for 1hr at 37 degrees C, stopping the reaction, and adding glutamate dehydrogenase to convert the glutamine produced to a-KGA. After incubation at room temperature for 45 min the absorbance at 340 nm was recorded for each sample. Insufficient information has been provided to include other interactions in this paper. rac1@cornell.edu Cancer Cell (1535-6108) 2010 968 5-[3-bromo-4-(dimethylamino)phenyl]-2,2-dimethyl-2,3,5,6-tetrahydrobenzo[a]phenanthridin-4(1H)-one small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) (5-[3-bromo-4-(dimethylamino)phenyl]-2,2-dimethyl-2,3,5,6-tetrahydrobenzo[a])is the name given in PMID: 20832749. glutamine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) glsk_mouse Glutaminase kidney isoform, mitochondrial Gls Gls1 Kiaa0838 protein protein mouse Mus musculus Mouse MMRLRGSAMLRELLLRPPAAVGAVLRRAQPLGTLCRRPRGGSRPTAGLVAAARLHPWWGGGGRAKGPGAGGLSSSPSEILQELGKGGTPPQQQQQQQQQPGASPPAAPGPKDSPGETDAFGNSEGKEMVAAGDNKIKQGLLPSLEDLLFYTIAEGQEKIPVHKFITALKSTGLRTSDPRLKECMDMLRLTLQTTSDGVMLDKDLFKKCVQSNIVLLTQAFRRKFVIPDFMSFTSHIDELYESAKKQSGGKVADYIPQLAKFSPDLWGVSVCTVDGQRHSIGDTKVPFCLQSCVKPLKYAIAVNDLGTEYVHRYVGKEPSGLRFNKLFLNEDDKPHNPMVNAGAIVVTSLIKQGVNNAEKFDYVMQFLNKMAGNEYVGFSNATFQSERESGDRNFAIGYYLKEKKCFPEGTDMVGILDFYFQLCSIEVTCESASVMAATLANGGFCPITGERVLSPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSGVAGGILLVVPNVMGMMCWSPPLDKMGNSVKGIHFCHDLVSLCNFHNYDNLRHFAKKLDPRREGGDQRVKSVINLLFAAYTGDVSALRRFALSAMDMEQRDYDSRTALHVAAAEGHVEVVKFLLEACKVNPFPKDRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDDGKGNQTVHKNLDGLL active_968_biotin 3-bromo-4-(dimethylamino) benzaldehyde_biotin small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) gac_glu-968 986314 n/a 986317 inhibitor inhibitor neutral component neutral component n/a 986318 enzyme target enzyme target substrate substrate neutral component neutral component n/a 986319 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig 5A. V max = 620 mol of glutamine hydrolyzed per minute per mol of enzyme. gac_glu-969 986315 n/a 986320 unspecified role unspecified role bait bait n/a 986319 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 5A. gac_glu-970 986316 n/a 986319 unspecified role unspecified role prey prey region VSPESSDDTSTTVVYR identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain false n/a 986320 unspecified role unspecified role bait bait physical association physical association false false false Fig S2A.